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Axon MultiClamp 700A Manuals
Manuals and User Guides for Axon MultiClamp 700A. We have
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Axon MultiClamp 700A manual available for free PDF download: Theory And Operation
Axon MultiClamp 700A Theory And Operation (159 pages)
COMPUTER-CONTROLLED MICROELECTRODE AMPLIFIER
Brand:
Axon
| Category:
Amplifier
| Size: 2.47 MB
Table of Contents
Declaration of Conformity
4
Table of Contents
9
Chapter 1: Introduction
13
Chapter 2: Installation and Basic Operation
15
Installation
15
Check List
15
Installing Hardware
16
Installing the Multiclamp Commander
16
Functional Checkout
18
Communication with the Multiclamp 700A
18
Setting Parameters in the Multiclamp Commander
18
Toolbar Buttons in the Multiclamp Commander
20
Test the Noise
21
Calibration
22
Getting Help in the Multiclamp Commander
24
Chapter 3: Using the Multiclamp 700A - Tutorials
25
Check List
25
Tutorial 1 - Electrode in the Bath: Voltage Clamp
26
Tutorial 2 - Electrode in the Bath: Current Clamp
29
Tutorial 3 - Giga Seal Configuration
30
Tutorial 4 - Whole-Cell Configuration: Voltage Clamp
35
Tutorial 5 - Whole-Cell Configuration: Current Clamp
39
Chapter 4: A Practical Guide to Electrophysiological Recording Using the Multiclamp 700A
43
General Advice
44
Chamber Design
44
Interfacing a Computer
46
Patch Clamping
47
Headstage and Holder Considerations
47
Forming a Gigaseal
48
Whole-Cell Voltage Clamp Recording
51
Perforated-Patch Recording
54
Low Noise Techniques
55
Sharp Microelectrode Recording
59
Sharp Microelectrode or Patch Electrode
60
Microelectrode Properties
60
Filling Solutions
64
Impaling Cells
64
Chapter 5: Reference Section
67
Audio Monitor
68
Bath Headstage and Electrodes
72
Bridge Balance
75
Buzz
78
Capacitance Compensation
79
Capacitance Neutralization
85
Clear
88
Electrochemistry
89
External Command Inputs
91
Feedback Resistor
93
Filters
95
Grounding and Hum
99
Headstage
101
Help
106
Holders
107
Input/Output Connections
111
Leak Subtraction
114
Mode
116
Model Cell
117
Noise
119
Oscilloscope Triggering
122
Output Zero
123
Overload
124
Polarity Conventions
125
Power Supply
127
Select Device
128
Series Resistance Compensation
129
Softpanel Configuration
142
Status
143
Zap
144
Chapter 6: Troubleshooting
145
Chapter 7: Specifications
147
References
153
Warranty and Repair Service
155
Shipping
157
Index
158
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