Axon AutoMate Scientific MultiClamp 700B Manuals

Axon AutoMate Scientific MultiClamp 700B Manuals

Manuals and User Guides for Axon AutoMate Scientific MultiClamp 700B. We have 1 Axon AutoMate Scientific MultiClamp 700B manual available for free PDF download: Manual

Axon AutoMate Scientific MultiClamp 700B Manual

Axon AutoMate Scientific MultiClamp 700B Manual (174 pages)

Computer-Controlled Micrpelectrode Amplifier

Brand: Axon | Category: Amplifier | Size: 1.73 MB

Table of Contents

Table of Contents
5
Chapter 1 Introduction

11
Chapter 2 Installation and Basic Operation

13
Chapter 3 Tutorials

25
Chapter 4 Guide to Electrophysiological Recording

53
- General Advice
  54
- Chamber Design
  54
- Perfusion
  54
- Mechanical Stability
  54
- Optics
  55
- Bath Electrode
  55
- Interfacing a Computer
  56
- Computer Noise
  57
- Patch Clamping
  57
- Headstage and Holder Considerations
  57
- Forming a Gigaseal
  59
- Whole-Cell Voltage Clamp Recording
  61
- Perforated-Patch Recording
  64
- Low Noise Techniques
  65
- Sharp Microelectrode Recording
  69
- Sharp Microelectrode or Patch Electrode
  70
- Microelectrode Properties
  70
- Filling Solutions
  74
- Impaling Cells
  74
Chapter 5 Reference Section

77
- Audio Monitor
  78
- Bath Headstage and Electrodes
  82
- Minimization
  82
- Use of a Bath Headstage
  84
- Bridge Balance
  85
- Bridge Balance in the Bath
  86
- Bridge Balance in the Cell
  87
- Buzz
  88
- Capacitance Compensation
  88
- Electrode Capacitance Compensation
  89
- Whole-Cell Capacitance Compensation
  90
- Auto Button
  93
- Manual Adjustment of Capacitance Compensation
  93
- Filtering the Command Stimulus
  94
- Capacitance Neutralization
  94
- Input Capacitance
  94
- Adjusting Capacitance Neutralization
  96
- Limitations of Capacitance Neutralization
  96
- Clear
  97
- Electrochemistry
  97
- External Command Inputs
  99
- External Command Sensitivity
  99
- Additivity of Commands
  100
- Command Filter Frequency
  100
- Feedback Resistor
  101
- V-Clamp Mode
  101
- I-Clamp Mode
  103
- Filters
  103
- Db Frequency
  104
- Types of Filters
  105
- Bessel Filter
  105
- Butterworth Filter
  105
- Choosing the Cutoff Frequency
  105
- High-Pass Filter
  107
- Command Filter Frequency
  107
- Grounding and Hum
  107
- Headstage
  108
- Voltage Clamp Circuit
  109
- Current Clamp Circuit
  111
- Mounting the Headstage
  112
- Bath Connection
  112
- Cleaning
  112
- Static Precautions
  112
- Acoustic Pick-Up
  112
- Help
  113
- Holders
  113
- Holder Design
  113
- Optional Ag/Agcl Pellets
  115
- Holder Use
  115
- Holder Maintenance
  117
- Adapters
  117
- Input/Output Connections
  117
- Front Panel
  117
- Rear Panel
  119
- Leak Subtraction
  120
- Mode
  121
- Model Cell
  122
- Noise
  124
- Measurement of Noise
  124
- Sources of Noise
  124
- Oscilloscope Triggering
  126
- Output Zero
  127
- Overload
  128
- Polarity Conventions
  128
- Biological Polarity Conventions
  128
- Multiclamp Polarity Conventions
  129
- Polarity Summary for Different Recording Configurations
  130
- Power Supply
  130
- Supply Voltage Selection
  131
- Changing the Fuse
  131
- Glitches
  131
- Select Device
  132
- Series Resistance Compensation
  132
- Introduction to R Compensation
  132
- Is R Compensation Necessary
  134
- Adjusting R Compensation
  135
- Theory of R Compensation
  139
- The 'Prediction' Control
  141
- Saturation Effects
  142
- Readjustment of Whole Cell Compensation with 'Prediction
  143
- The 'Correction' Control
  144
- Readjustment of Whole Cell Compensation with 'Correction
  145
- Setting 'Prediction' and 'Correction' Values
  145
- Oscillations
  145
- Limitations of R Compensation
  146
- Softpanel Configuration
  147
- Status
  148
- Zap
  148
Chapter 6 Troubleshooting

149
Chapter 7 Specifications

151
- References
  157
- Technical Assistance
  159
- Warranty and Repair Service
  161
- Circuit Diagrams Request Form
  165
- Declaration of Conformity
  167
- Important Safety Information
  169
- Index
  173

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