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Axopatch-1D
Axon Axopatch-1D Manuals
Manuals and User Guides for Axon Axopatch-1D. We have
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Axon Axopatch-1D manual available for free PDF download: Theory And Operation
Axon Axopatch-1D Theory And Operation (94 pages)
Brand:
Axon
| Category:
Amplifier
| Size: 0.51 MB
Table of Contents
Table of Contents
11
List of Diagrams
16
Introduction
19
Features
21
Functional Checkout
25
Getting up and Running - a Tutorial
27
Reference Guide
37
Audio Monitor
37
Suggested Use
37
Bath Headstage
37
Non-Use
38
Capacitance Compensation
38
Pipette Capacitance Compensation
38
Suggested Use
38
Membrane Capacitance Compensation
39
Ss = 0.1 for Large Cells
40
Suggested Use
40
Limitations
41
Command Potentials
41
Oscillator
41
Step Command
41
Holding Potential
41
Test
41
Ext. Command Sensitivity
42
Speed Test
42
Command Summing
42
Current and Voltage Conventions
42
Positive Current
43
Inward Current
43
Outward Current
43
Positive Potential
43
Transmembrane Potential
43
Depolarizing / Hyperpolarizing
43
Whole-Cell Voltage and Current Clamp
43
Patch Clamp
44
Summary
45
Current Clamp
46
Membrane Potential
46
Whole-Cell Current Clamp
46
Series Resistance
47
Capacitance Compensation
47
Limitations
48
Current Range
48
Suggested Use
48
Grounding and Hum
49
Headstages
50
Principles of Operation
50
If the Probe Output Is Slow, How Can Voltage Clamping be Fast
50
What Is Clamped
51
Gain Selection
51
Which Headstage Should You Use
51
Tuning CV-4 Headstages
52
Offset Adjustment for CV-4
52
Headstage Gain Adjustment
53
Case Ground Connector
54
Mounting the Headstage
54
Cleaning
54
Static Precautions
54
Optical Pick-Up
54
Where to Place the CV-4 Boost Box
54
Other Precautions
55
Pipette Holder
55
Features
55
Parts
55
Use
56
Glass Dimensions
57
Adapters
57
Junction Null
57
Implementation
59
Errors Due to Nonzero Headstage Error Current
59
Suggested Use
59
Main Meter
60
Mode
60
Model Cell
60
Description
60
Use
61
Noise
62
Oscilloscope Triggering
63
Suggested Use
63
Outputs Section
63
Filter
63
Gain (Α)
64
Frequency and Gain Setting Outputs
64
Output Selection
64
Leak Subtraction
64
Auto Output Zero
65
Distortion-Free AC Coupling
65
Principle of Operation
65
Output Calibration
65
Internal
66
External
66
Suggested Use
66
Pipettes
66
Glasses
66
Pulling
67
Coating
67
Filling
67
Power Supply Glitches
68
Power Supply Voltage and Fuse Changing
68
Supply Voltage
68
Changing the Fuse
69
RMS Noise Display
69
Purpose
69
RMS Meter
69
Bandwidth
69
Expected Values
70
Seal Formation
70
Series Resistance
71
Absolute Value
71
Compensation
71
Saturation
72
Oscillations
72
Using Lag to Prevent Oscillations
72
Suggested Use
73
Ss = 0.1 for Large Cells
73
Limitations
73
Ten-Turn Potentiometers
74
Trouble Shooting
74
Zap
74
Suggested Use
74
Specifications
75
Service
81
General
81
Maintenance
85
Adjustment Procedure
86
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