Axon Axopatch-1D Patch Clamp Amplifier Manuals

Manuals and User Guides for Axon Axopatch-1D Patch Clamp Amplifier. We have 1 Axon Axopatch-1D Patch Clamp Amplifier manual available for free PDF download: Theory And Operation

Axon Axopatch-1D Theory And Operation

Axon Axopatch-1D Theory And Operation (94 pages)

Brand: Axon | Category: Amplifier | Size: 0 MB

Table of Contents

Table of Contents
11
List of Diagrams
16
Introduction
19
Features
21
Functional Checkout
25
Getting up and Running - a Tutorial
27
Reference Guide
37
- Audio Monitor
  37
  - Suggested Use
    37
- Bath Headstage
  37
  - Non-Use
    38
- Capacitance Compensation
  38
  - Pipette Capacitance Compensation
    38
  - Suggested Use
    38
  - Membrane Capacitance Compensation
    39
  - Ss = 0.1 for Large Cells
    40
  - Suggested Use
    40
  - Limitations
    41
- Command Potentials
  41
  - Oscillator
    41
  - Step Command
    41
  - Holding Potential
    41
  - Test
    41
  - Ext. Command Sensitivity
    42
  - Speed Test
    42
  - Command Summing
    42
- Current and Voltage Conventions
  42
  - Positive Current
    43
  - Inward Current
    43
  - Outward Current
    43
- Positive Potential
  43
- Transmembrane Potential
  43
- Depolarizing / Hyperpolarizing
  43
- Whole-Cell Voltage and Current Clamp
  43
- Patch Clamp
  44
- Summary
  45
Current Clamp
46
- Membrane Potential
  46
- Whole-Cell Current Clamp
  46
- Series Resistance
  47
- Capacitance Compensation
  47
- Limitations
  48
- Current Range
  48
- Suggested Use
  48
Grounding and Hum
49
Headstages
50
- Principles of Operation
  50
- If the Probe Output Is Slow, How Can Voltage Clamping be Fast
  50
- What Is Clamped
  51
- Gain Selection
  51
- Which Headstage Should You Use
  51
- Tuning CV-4 Headstages
  52
- Offset Adjustment for CV-4
  52
- Headstage Gain Adjustment
  53
- Case Ground Connector
  54
- Mounting the Headstage
  54
- Cleaning
  54
- Static Precautions
  54
- Optical Pick-Up
  54
- Where to Place the CV-4 Boost Box
  54
  - Other Precautions
    55
- Pipette Holder
  55
  - Features
    55
  - Parts
    55
  - Use
    56
  - Glass Dimensions
    57
  - Adapters
    57
- Junction Null
  57
  - Implementation
    59
  - Errors Due to Nonzero Headstage Error Current
    59
  - Suggested Use
    59
- Main Meter
  60
- Mode
  60
- Model Cell
  60
  - Description
    60
  - Use
    61
- Noise
  62
- Oscilloscope Triggering
  63
  - Suggested Use
    63
- Outputs Section
  63
  - Filter
    63
  - Gain (Α)
    64
  - Frequency and Gain Setting Outputs
    64
  - Output Selection
    64
  - Leak Subtraction
    64
  - Auto Output Zero
    65
  - Distortion-Free AC Coupling
    65
  - Principle of Operation
    65
- Output Calibration
  65
  - Internal
    66
  - External
    66
  - Suggested Use
    66
- Pipettes
  66
  - Glasses
    66
  - Pulling
    67
  - Coating
    67
  - Filling
    67
- Power Supply Glitches
  68
- Power Supply Voltage and Fuse Changing
  68
  - Supply Voltage
    68
  - Changing the Fuse
    69
- RMS Noise Display
  69
  - Purpose
    69
  - RMS Meter
    69
  - Bandwidth
    69
  - Expected Values
    70
- Seal Formation
  70
- Series Resistance
  71
  - Absolute Value
    71
  - Compensation
    71
  - Saturation
    72
  - Oscillations
    72
  - Using Lag to Prevent Oscillations
    72
  - Suggested Use
    73
  - Ss = 0.1 for Large Cells
    73
  - Limitations
    73
- Ten-Turn Potentiometers
  74
- Trouble Shooting
  74
- Zap
  74
  - Suggested Use
    74
Specifications
75
Service
81
- General
  81
- Maintenance
  85
- Adjustment Procedure
  86

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