Meiji Techno TC5000 Series Instruction Manual
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Meiji Techno TC5000 Series Instruction Manual

Inverted biological microscope
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  • Page 1 TC5000 Series Inverted Microscope Instruction Manual...

  • Page 2: Table Of Contents

    Table of Contents 1.0 Introduction 1.1 Microscope Features 1.2 General Safety Guidelines 1.3 Warning/Caution symbols used in this manual 1.4 Intended Product Use Statement 1.5 Product Safety Information - Handling the microscope 1.6 Warranty Notes 2.0 The Microscope and its Components 2.1 Installation Site 2.2 Unpacking 2.3 Microscope Set Up...

  • Page 3: Introduction

    Contrast methods available include: Brightfield, Phase Contrast and Epi-Fluorescence. Meiji Techno supplies a variety of standard specimen holders for the TC to accommodate many types of slides, cell culture glassware and well plates.

  • Page 4: Microscope Features

    1.2 General Safety Guidelines This manual contains important safety instructions and information concerning the installation, operation and maintenance of the Meiji Techno TC5000 Series inverted biological microscopes. This manual should be read carefully before any attempt is made to operate this equipment.

  • Page 5: Warning/Caution Symbols Used In This Manual

    In no event shall Meiji Techno be liable to any person for any incidental, indirect or consequential damages, arising out of or in connection with the use or...

  • Page 6: Product Safety Information- Handling The Microscope

    1.5 Product Safety Information- Handling the Microscope This microscope is not intended to be used in the immediate vicinity of water or a water outlet or placed in any location where water may penetrate the instrument. Water penetration may result in electrical shock or death. Choose only a suitable environment for your microscope.

  • Page 7: Warranty Notes

    No charge will be made for labor or materials with respect to defects covered by this warranty, provided all repair work is done by Meiji Techno. This warranty does not cover expenses incurred in the removal or reinstallation of any instrument or instruments, whether or not proven defective.
  • Page 8 Meiji Techno’s convenience. In some cases, requests for warranty service are received which are not justified. In these cases, Meiji Techno will provide an explanation for non- warranty action. WARRANTY TERMS The terms of this warranty may not be varied by any person, whether or not purporting to represent or act on behalf of Meiji Techno.

  • Page 9: The Microscope And Its Components

    2.0 The Microscope and its Components The image below designates the main components of the TC5100 Series Brightfield Inverted Microscope. 1. Binocular Head, 30° Inclination 2. Eyepieces, 10, 15, 20X available 3. Beamsplitter for Front Camera Port 4. Aperture Diaphragm 5.
  • Page 10 The image below designates the main components of the TC5300 Series Phase Contrast Inverted Microscope. Binocular Head, 30° Inclination 2. Eyepieces, 10, 15, 20X available 3. Phase Slider 4. Aperture Diaphragm 5. Extended Working Distance Condenser 6. Specimen stage 7. Quadruple Nosepiece & Phase Objectives 8.
  • Page 11 The image below designates the main components of the TC5500 Epi- Fluorescence Inverted Microscope. Binocular Head, 30° Inclination 2. Eyepieces, 10, 15, 20X available 3. Filter Slider 4. Aperture Diaphragm 5. Extended Working Distance Condenser 6. Specimen stage 7. Quadruple Nosepiece & Phase Objectives 8.

  • Page 12: Installation Site

    2.1 Installation Site The microscope should be operated in a room with as little dust as practically possible. Keep your instrument away from solvents, chemical fumes and excessive humidity. Also try to avoid big swings in ambient temperature, direct sunlight and vibration as they can affect measurements and instrument performance.

  • Page 13: Microscope Set Up

    2.3 Microscope Set Up • As a first step, remove all components from the shipping container and remove the packing materials. Save the container and packing in a dry location. • Place the microscope frame on a stable work surface. •...
  • Page 14 • If purchased, un-wrap the mechanical stage (MA380/05). Locate the two holes on the bottom side of the plain stage on the right hand side. The two thumbscrews will attach the mechanical stage to the plain stage as shown in Figure 6. Tighten the thumbscrews snugly.
  • Page 15 2.4 Installing the Phase Contrast Slider These instructions are only to be followed if you currently have a Model TC-5100, TC-5200, TC-5500 and TC-5600 and you wish to upgrade your microscope to perform Phase Contrast Microscopy. In addition to installing Phase Contrast objectives, the Phase Contrast Slider will also have to be installed.

  • Page 16: Installing Fluorescence Option

    2.5 Installing Fluorescence Option The following assembly steps are not required if fluorescence accessories have NOT been purchased. Prior to installing the fluorescence module into the microscope body; one will need to install the filters and mirrors into their slide carriage or holder. Installing the Filters and the Fluorescence Module The filter block slide can accommodate two different filter sets.
  • Page 17 3. Next, remove the fluorescence access cover on the back of the microscope as shown in Figure 2a using a #2 Phillips screwdriver removing all seven screws. Store the four smaller screws in a safe place along with the access cover as they will not be used with this configuration.
  • Page 18 12. When installed correctly, the fluorescence filter carriage will slide easily and click and lock in all three positions. When using the microscope, be careful when switching filter positions when the light source is on. There is a risk of light escaping while switching between filters, so be careful not to directly look at the escaping light.

  • Page 19: Fluorescence Filters Information

    2.6 Fluorescence Filter Information The epi-fluorescence equipped TC-5500 and TC-5600 include the following filter sets: Excitation Type Exciter Filter Dichroic Mirror Emitter Filter Applications 25mm diameter 25.7mm X 25mm diameter 36mm Blue D470/40x 495DCLP E515LPv2 FITC Chroma P/N: Acridine Orange Auramine 11001v 2 EGFP, S65T,...
  • Page 20 3.0 Operation Once the microscope has been setup in its working location with all of the components correctly installed, it is ready for use. Your TC-5000 is a precision instrument designed to last a lifetime. Always handle your microscope with care and avoid abrupt motion, vibration and shock. Do not install any bulb in your instrument other than the ones designated by Meiji Techno: MA326 6V 30W Halogen...
  • Page 21 3.1 Basic Set Up For Transmitted Light – Brightfield 1. First, set your interpupillary distance on the microscope eyetubes by pulling them apart or pushing them closer together to fit your eyes. When set correctly, one will see one uniform round field of view. Make note of the distance setting when adjusted to your liking so you can later repeat the setting.
  • Page 22 Objectives Information Depending on which objective is being used, before turning the nosepiece to change magnifications, the nosepiece will need to be lowered with the course focus knob otherwise the objective may crash into the stage if caution is not used. Possible Brightfield Mode Operational Problems If normal adjustments are not getting the results you expect, check to see if these conditions exist:...

  • Page 23: Transmitted Light Operation - Phase Contrast

    Phase contrast is a very useful technique for high-contrast images of unstained or transparent specimens in petri dishes, cell and tissue culture flasks and multi-well plates. Meiji Techno’s TC5000 Series Phase Contrast Model features a centerable phase contrast slider with 4X annulus, 10X-20X-40X annulus and an empty slot for brightfield.
  • Page 24 Possible Phase Contrast Mode Operational Problems If normal adjustments are not getting the results you expect, check these: • Wrong phase annulus being used. • Phase annulus not centered correctly. • Wrong condenser installed. • Aperture diaphragm is completely closed. •...

  • Page 25: Incident Light Operation: Epi-Fluorescence

    3.3 Incident Light Operation: Epi-Fluorescence For microscopes with fluorescence option installed only. For viewing of transparent objects using incident-light fluorescence, we recommend to first make your initial adjustments using brightfield with transmitted light. 1. First, switch on the power supply to the Nobska lamphouse and allow the lamp to achieve operating temperature.
  • Page 26 Possible Incident Light Fluorescence Operational Problems If normal adjustments are not getting the results you expect, check these: • Weak fluorescence or image intensity: specimens improperly stored or too old • Rapid fading of specimens i.e.: photobleached • Unspecific or erroneous filter combination •...

  • Page 27: Uv Light Safety Considerations

    3.4 UV Light Safety Considerations Mercury arc lamps have UV light content in their output. The U.S. National Institute for Occupational Safety and Health (NIOSH) recommends that exposure to UV energy be controlled and limited as much as practically possible. Exposure to UV radiation even for very brief periods of time can be hazardous.

  • Page 28: Photomicrography With 35Mm Slr And Digital Slr Cameras

    3.5 Photomicrography with 35mm SLR and Digital SLR Cameras The TC Series of microscopes have a camera port directly in front of the instrument. In order to secure a 35mm SLR camera body to this microscope, an optional camera attachment part number MA869 will need to be attached to the front camera port of the TC with the corresponding T2 Adapter Ring that matches the camera to be used.

  • Page 29: Photomicrography With Digital Still Cameras

    3.6 Photomicrography with Digital Still Cameras In order to mount a consumer grade digital camera to this microscope, an optional camera attachment MA868 plus a camera adapter will be needed to attach to the front camera port of the TC. The table below shows the different cameras that can be used and their corresponding DC Series adapter: Camera Nikon...

  • Page 30: Maintenance And Cleaning

    4.0 Maintenance and Cleaning • Disconnect the power cord on your equipment prior to performing cleaning, maintenance or repair. • Keep electrical components away from moisture or humidity. • In warm humid climates, take special care to prevent your equipment from exposure to fungal growth.
  • Page 31 Cleaning of Glass Surfaces Use a soft brush or lint-free cotton cloth to removed dust and loose particles. For tough dirt, use a soft lint-free cotton cloth moistened with distilled water. If that fails, try using medical or reagent grade isopropyl alcohol. Cleaning the Objectives Objectives should NEVER be disassembled for cleaning or for any other reason! We do not advise cleaning the inside surfaces of objectives or eyepieces.

  • Page 32: Troubleshooting

    5.0 Troubleshooting Meiji Techno products are manufactured in Japan under ISO9000 manufacturing standards. However, if you ever have any difficulty with any Meiji product, feel free to contact us at: Meiji Techno America 2186 Bering Drive San Jose, CA 95131 800.832.0060...

  • Page 33: Replacing The Mains Fuse On The Microscope

    5.1 Replacing the mains fuse on the microscope ALWAYS DISCONNECT YOUR EQUIPMENT BEFORE DOING ANY REPAIR. Location of Mains Fuse The mains fuse of the TC Series Inverted Microscopes is located on the back panel of the microscope as shown. Instructions to replace the mains fuse: •...

  • Page 34: Replacing The 6V 30W Halogen Light

    5.3 Replacing the 6V 30W Halogen Light ALWAYS DISCONNECT YOUR EQUIPMENT BEFORE DOING ANY REPAIR. Do not touch the glass envelope of the lamp during installation. Keep the protective sleeve or bag of the lamp during installation and remove it right after installation. LAMP AND LAMP HOUSING MAY BE HOT TO THE TOUCH.

  • Page 35: Fluorescence Lamp Does Not Work

    5.4 Fluorescence Lamp does not work LAMP HOUSE MAY BE HOT TO THE TOUCH. Do not touch the glass envelope of the lamp at any time. Keep the protective sleeve or bag of the lamp during installation and remove it right after installation. •...

  • Page 36: Storage

    6.0 Storage Protect your microscope from dust after each use by covering your instrument with the protective dust cover that came with your microscope. Store your microscope in a cabinet that has a stable temperature and low humidity. If you live in an area that has high humidity, consider storing your microscope in a sealed container along with a desiccant such as silica gel.

  • Page 37: Accessories And Replacements Parts

    Microscopes can be found listed below. Accessories and replacement parts for all Meiji Techno products are available through our dealer network. Feel free to contact us a call so we may direct you to the closest authorized Meiji Techno Dealer in your area.
  • Page 38 Epi-Fluorescent Objectives - Planachromat - Infinity Corrected - F = 200mm TC Planachromat F4X objective ∞/1.0, NA: 0.13, MA849 WD = 17.5mm, F.L.= 50.0 (optional) TC Planachromat F10X objective ∞/1.0, NA: 0.30, MA850 WD = 7.5mm, F.L.= 20.0 (included) TC Planachromat F20X objective ∞/1.0, NA: 0.40, MA851 WD = 7.5mm, F.L.= 10.0 (included) TC Planachromat F40X objective ∞/1.0, NA: 0.60,...
  • Page 39 MA860 ND25 Neutral density filter for epi-illuminator (included with TC5500, TC5600)

  • Page 40: Technical Descriptions

    9.0 Technical Descriptions Filters and Applications Filter Application ND25 Neutral Filter or ND Filter. Grey filters or neutral density filters are used to attenuate all frequencies of light equally resulting in preservation of color temperature. The ND25 filter would indicate a reduction of light transmission by 75 percent or a passage of light of 25 percent.

  • Page 41: Physical Dimensions

    10.0 Physical Dimensions...
  • Page 42 Meiji Techno Co. Ltd. Toll free: (800) 832-0060 MEIJI TECHNO AMERICA Phone: (408) 428-9654 2186 Bering Drive Fax: (408) 428-0472 San Jose, CA 95131 sales@meijitechno.com Email: Web: http://www.meijitechno.com MEIJI TECHNO CO., LTD. Phone: 049-259-0111 322-1, Chikumazawa, Fax: 049-259-0113 Miyoshi machi, Iruma-gun E-mail: meiji@meijitechno.co.jp...

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