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Note: Certain fluorographic agent
manufacturers specify tempera-
tures higher than those reached
in the Hoefer Easy Breeze (e.g.
Autofluor
™
[National Diag-
nostics] and Fluoro-Hance
[Research Products Interna-
tional], which require up to
80 °C to produce an optimum
signal). This dryer is not recom-
mended for such agents.
5. Visualization Notes
Direct Autoradiography
Prepare the gel in the usual manner and expose
to film as directed with the instructions accom-
panying the film. Exposure times, especially for
medium energy beta-emitters such as
can be quite long. For maximum sensitivity of
these types of labels, use fluorography.
Fluorography
Fluorography intensifies a weak beta emission
signal by impregnating the gel with a suitable
fluor that converts beta emission from
and
does not quench this signal, but for
weak beta-emitter, up to 20% of the signal may
be lost.
If using EN
fluorography agents, include 0.5% to 2% glyc-
erol in the cold water treatment prior to drying.
Dehydration steps are not required for thinner,
lower percentage gels, which are far less prone
™
to cracking. If exposed to the maximum drying
temperature (~54 °C), these gels may form longer
fluor crystals, resulting in higher background on
X-ray exposures.
3
H into light. Drying the gel in cellophane
3
HANCE or sodium salicylate as
14
35
C and
S
35
14
S,
C,
3
H, a very
•
p15
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