Related Manuals for PreSens pH-10 mini
Summary of Contents for PreSens pH-10 mini
- Page 1 PreSens Precision Sensing GmbH Instruction Manual pH-10 mini 10-Channel Minisensor pH-Meter...
- Page 2 PreSens Precision Sensing GmbH Instruction Manual pH-10 mini 10-Channel Minisensor pH-Meter Manual version 3 PC software version pH10-10_v6 PreSens Precision Sensing GmbH Josef-Engert-Strasse 11 D-93053 Regensburg Germany Phone +49 941 942 72 0 +49 941 942 72 27 Email info@PreSens.de Internet www.PreSens.de...
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Page 3: Table Of Contents
Safety Guidelines ....................4 Description of the pH-10 mini Device................6 Instrument....................... 6 Front Panel of the pH-10 mini Device..............6 Rear Panel of the pH-10 mini Device ..............7 Required Basic Equipment..................8 pH-Sensitive Minisensors ..................... 9 General Description ....................9 Dynamic range...................... - Page 4 Content Some Advice for Correct Measurement..............29 Signal drift due to photo-decomposition ............29 Performance proof ....................29 General Instructions: ....................30 Warm-Up Time ...................... 30 Maintenance......................30 Service........................30 Technical Data General Data..................31 Technical Notes....................33 Operation Notes ....................33 Appendix........................
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Page 5: Preface
Congratulations! You have chosen a new, innovative technology for measuring pH! The pH-10 mini is a compact, easy to transport and completely PC-controlled fiber optic pH meter. The data evaluation is PC supported as well. The pH-10 mini was specially developed for small fiber optic pH sensors, flow-through cells and integrated sensor systems. -
Page 6: Safety Guidelines
• has been stored under adverse conditions for a lengthy period of time • has been damaged in transport. If you are in doubt, the instrument should be sent back to the manufacturer PreSens for repair and maintenance. The operator of this measuring instrument must ensure that the following laws and guidelines are observed when using dangerous substances: •... - Page 7 We explicitly draw your attention to the fact that any damage of the manufactural seal will render all guarantee warranties invalid. Any internal operations on the unit must be carried out by personnel explicitly authorized by PreSens, and under antistatic conditions.
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Page 8: Description Of The Ph-10 Mini Device
Description of the pH-10 mini Device 2 Description of the pH-10 mini Device 2.1 Instrument The pH-10 mini is a precision pH meter, designed for fiber-optic pH minisensors. For operation, a PC/Notebook with RS- 232 interface is required. The pH-10 mini... -
Page 9: Rear Panel Of The Ph-10 Mini Device
Line Blue Control off: instrument off 2.3 Rear Panel of the pH-10 mini Device The electrical specifications of all rear panel connectors are given in the technical specification sheet. Please read also the technical notes to avoid mistakes. ELEMENT DESCRIPTION... -
Page 10: Required Basic Equipment
Required Basic Equipment 2.4 Required Basic Equipment • pH meter pH-10 mini* • Software for pH-10 mini* • PC / Notebook (System requirements: Windows XP; Pentium processor, at least 166 MHz, 64 MB RAM, A RS 232 port or a USB port and a USB-serial adapter is needed) •... -
Page 11: Ph-Sensitive Minisensors
3 pH-Sensitive Minisensors 3.1 General Description Optical pH Sensors designed for pH-10 mini device are based on the new Dual Luminophor Reference method (see also appendix). They consist of an inert long decay time reference dye and a short decay time indicator dye, which changes its fluorescence intensity due to the pH-value. -
Page 12: Characteristics Of A Typical Ph Sensor
pH-Sensitive Minisensors 3.3 Characteristics of a typical pH sensor The characteristic of a typical sensor are listed below: Dynamic range: pH 5.5 to 9 Resolution: 0.01 pH Drift due to bleaching: 0.003 pH per 1000 measuring points Response time: less then 30 sec Please note that the given values may differ from your special device and sensoric application. -
Page 13: Sensor Drift
pH-Sensitive Minisensors 3.5 Sensor drift Like all optical chemo sensors, optical pH-sensors are subject to photobleaching. Due to this process the measured intensity (amplitude) is decreasing while the phase of the sensor is increasing. This process is quite slow and depends on the light intensity used and the duration of illumination. -
Page 14: Limitations Of Optical Ph Sensors
Minisensors 3.7 Limitations of optical pH sensors The pH sensors offered for pH-10 mini were specially designed for physiological samples and media. Samples with extremely low ionic strength and low buffer content may be not measured. To test whether the sample can be measured accurately, try to perform the calibration procedure with a buffer system which is as similar to the sample as possible. -
Page 15: Planar Analyte-Sensitive Foils
Sterilizable by gamma irradiation • Insensible towards electrical interference and magnetic fields • Many disposables with integrated pH Sensors are available Planar pH sensor spots can be used in combination with the pH meter pH-1 mini, pH-4 mini and pH-10 mini... -
Page 16: Flow-Through Cell - Ph Minisensor
T- shape flow-through cell. The FTC is connected to the PreSens pH meter pH- 10 mini, pH-4 mini or pH-1 mini by a polymer optical fiber (POF) with 2 mm diameter as a light guide. The volume for liquid inside the FTC is about 300 microliters. - Page 17 Insensible towards electrical interferences and magnetic fields • This Flow-Through Cell minisensor can be used in combination with the pH meter pH-1 mini, pH-4 mini and pH-10 mini. Order code for pH Flow-Trough cells The order code key of the Flow-Through cell is shown below.
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Page 18: Software
General Instructions 4 Software 4.1 Software Installation This software is compatible with Windows XP. 1. Insert the supplied disc/CD into the respective drive. Start the file setup.exe 2. Follow the instructions of the installation wizard 3. Choose the folder for installing the software... - Page 19 General Instructions Now the Software is properly installed and you will find a link in your Start menu.
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Page 20: Starting Ph-10 Mini And Its Software
General Instructions 4.2 Starting pH-10 mini and its Software 1. Connect the pH-10 mini via the supplied RS-232 serial cable to a serial port of your computer. Tighten the cable with the screws to your computer. 2. Connect the power supply. -
Page 21: Function And Description Of The Ph-10 Mini Software
General Instructions 4.3 Function and Description of the pH-10 mini Software The window shown below is displayed after starting the pH-10 mini software: 4.3.1 Menu Bar: Clear Graph This function clears the graph window from all channels as well as the individual channel. It is not possible to clear the graph window while a measurement is running. -
Page 22: Status Bar
General Instructions Calibration Here you can enter the calibration data of your pH sensors – either individual sets of calibration data for each sensor or the same set of calibration data for all channels. You can find the detailed description of calibration of pH sensors in chapter 6 “Calibration”. Exit: Here you can close the software 4.3.2 Status Bar... - Page 23 General Instructions You can log the measurement of all channels by selecting the “create all” button. You will be asked to chose a filename. Each Channel will be stored in a separate file. The software will create files with the ending ***-01.txt to ***-10.txt Alternatively you can log each channel individually by activating the respective channel at the square on the right side...
- Page 24 General Instructions All Channels Here you get a graphical display of all channels. You can add or remove graphs by activating them at the selection bar. You can zoom in the graph by moving the mouse with pressed left buttom from the upper left corner to the lower right corner and un-zoom vice versa.
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Page 25: Channel Selection
General Instructions 4.3.4 Channel selection Here you can select which channel is shown in the Status bar. The Graph of the respective channel also is shown. You can zoom in the graph by moving the mouse with pressed left button from the upper left corner to the lower right corner and un-zoom vice versa. In Report you can find information about the channel and the raw data. -
Page 26: Data Handling
Remove the protective cap from the male fiber plugs of the delivered fiber cable and connect it to the SMA-plugs of the pH-10 mini device. The safety nut must be carefully attached while turning slightly clockwise. Fix the other end of the fiber to the outer wall of your vessel, facing the sensor spot on the inner side of the vessel. -
Page 27: Measurement Of A Ph Minisensors
General Instructions plugs of the pH-10 mini device. The safety nut must be carefully attached while turning slightly clockwise. Connect the brass hull of the optical fiber to the FTC: Brass hull of the fiber Flow through cell Connected FTC Please be careful not to scratch the bare fiber of the Flow through cell. -
Page 28: Calibration
General Instructions 6 Calibration 6.1 Calibration You can enter the calibration data of your pH sensors – either individual sets of calibration data for each sensor or the same set of calibration data for all channels by choosing “Calibration” in the menu bar. You can find the calibration data of your sensor on the data sheet of your sensor. -
Page 29: Determination Of Calibration Constants
Connect the pH-10 mini via the RS232 cable to your computer. Switch on the pH-10 mini and connect the sensor as shown before depending on the sensor in use. Start the pH-10 mini software on your computer and start the measurement. - Page 30 General Instructions Type in pH and phase values Press the "Least Square Fit” button. The calibration results φ , φ , dpH, pH are shown and the calibration curve is displayed. Save your set of calibration values by pressing “Transfer Data”. Enter the new calibration constants and the temperature in the calbriation window.
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Page 31: Some Advice For Correct Measurement
General Instructions 7 Some Advice for Correct Measurement 7.1 Signal drift due to photo-decomposition pH – sensors are subject to photobleaching which results in a drift of the sensor to lower pH. Photobleaching depends at the amount of light. Therefore you should reduce the amount of measurement points by choosing longer intervals for long term measurements Bleaching always pretends lower pH. -
Page 32: General Instructions
General Instructions 8 General Instructions: 8.1 Warm-Up Time The warm up time of the electronic and opto-electronic components of the pH-10 mini is 5 min. Afterwards stable measurement values are obtained. 8.2 Maintenance The instrument is maintenance-free. The housing should be cleaned only with a moist cloth. Avoid any moisture entering the housing! Never use benzine, acetone, alcohol or other organic solvents. -
Page 33: Technical Data General Data
Concluding Remarks 9 Technical Data General Data MODES range: 5.5 – 9 resolution: 0.01 pH units OPTICAL OUTPUT / INPUT Optical connector Channels Wavelength 470 nm AC-Range : AC INPUT 220 V DIGITAL OUTPUT communication protocol serial interface RS232 38400 Baud, Databits 8, Stoppbits 1, Parity none, Handshake none on RJ11 4/4 plug instrument output:... - Page 34 Concluding Remarks ENVIRONMENTAL CONDITIONS Operating temperature 0 to +50ºC -10 to +65ºC Storage temperature up to 95% Relative humidity: OPERATION CONTROL LED at the front panel: DIMENSIONS length: 300 mm width: 250 mm height: 140 mm weight: 3600 g...
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Page 35: Technical Notes
Always use the rubber cap to close the output when not in use. 9.2 Operation Notes Measurement To achieve the highest accuracy the pH-10 mini should be warmed-up for 5min before starting the measurement. Please see the details of measurement process described in pH- 10 mini manual. -
Page 36: Appendix
PreSens uses new and general logic to reference fluorescence intensity signals by decay time measurement. In contrast to the most common ratiometric method, where luminescence excitation or emission is measured at two wavelengths, this scheme uses a couple of luminophores with different decay times and similar excitation spectra. - Page 37 Appendix overall signal overall signal reference reference indicator indicator (=LED) (=LED frequency) Φ Φ Φ Φ Φ Φ Φ Φ [°] [°] Phase shift of the overall luminescence (Φm), the reference (Φref) and the indicator (Φind). Fluorescence of the indicator in (A) absence and (B) presence of the analyte. Equations 5 and 6 show the superposition of the phase signals of the reference dye, which possess a constant decay time and luminescence intensity, and the indicator: ⋅...
- Page 38 Appendix Dividing equation 11 by 12 results in a correlation of the phase angle (Φ ) and the intensity ratio of the indicator dye (A ) and reference luminophore (A ⋅ Φ ⋅ Φ Φ Φ ⋅ (13) ⋅ Φ ⋅...
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