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Turner Designs C3 User Manual

Submersible fluorometer
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User's Manual
September 19, 2011
P/N: 998-2300
Revision H
TURNER DESIGNS
845 W. Maude Ave.
Sunnyvale, CA 94085
Phone: (408) 749-0994
FAX: (408) 749-0998

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  • Page 1 User’s Manual September 19, 2011 P/N: 998-2300 Revision H TURNER DESIGNS 845 W. Maude Ave. Sunnyvale, CA 94085 Phone: (408) 749-0994 FAX: (408) 749-0998...

  • Page 2: Table Of Contents

    Warranty Service Out of Warranty Service Appendices C3 Submersible Fluorometer Specifications C3 Submersible Fluorometer Recommended Practices C3 Submersible Fluorometer Wiring and Cable Guide Linear Range, Quenching and Temperature Considerations C-FINS Installation Guide Installing the Battery Bracket Connecting the Submersible Battery Pack...
  • Page 3 C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste...

  • Page 4: Introduction

    C3 Submersible Fluorometer 1. Introduction 1.1 The C3 Submersible Fluorometer is manufactured according to users’ optical specifications with one, two, or three optical sensors ranging in wavelengths from deep ultraviolet to infrared. Each optical sensor is designed with fixed excitation and emission filters. The C3 Submersible Fluorometer comes with a...

  • Page 5: Important Note Regarding The Use Of The Fluorescein Sensor

    Chlorophyll and/or Rhodamine sensors or it will produce unreliable results. For C3’s manufactured after 2/15/2011 this interference issue was corrected. If you would like to send your C3 back to Turner Designs to resolve this interference issue, please contact sales@turnerdesigns.com 998-2300 Rev.

  • Page 6: Inspection And Setup

    C3 Submersible Fluorometer 2. Inspection and Setup 2.1 Instrument Checklist The C3 Submersible Fluorometer package (P/N: 2300-000) includes: C3 Submersible Fluorometer Factory-installed temperature sensor Interface cable for communicating with your PC and supplying power to the instrument CD including C-Soft software, user’s manual, and quick start guide...

  • Page 7: Optional Accessories

    2.2 Optional Accessories Pressure sensor (P/N: 2300-360) Factory-installed (Note: Can only be installed during the manufacturing of the unit.) C3 Mechanical Wiper (P/N: 2300-450) Factory-installed (Note: Can only be installed during the manufacturing of the unit.) Replacement Wiper Brushes (P/N: 2300-460)
  • Page 8 C3 Submersible Fluorometer Computer Integration Cable Computer Integration Cable C-ray Towed Deployment Body C-ray Towed Deployment Body yellow heat shrink yellow heat shrink Flow Cap Flow Cap Battery Bracket Battery Bracket Shade Cap Shade Cap Air Purge Slots Air Purge Slots...

  • Page 9: Identification

    Sensors are marked with numbers 1, 2, or 3 located adjacent to the optics on the face of the C3 Submersible Fluorometer. These numbers correspond to channels 1, 2, and 3 in C-Soft (C3 software). Note: Channel names can be modified using C-Soft.

  • Page 10: C-Soft Windows™ User Interface

    USB to serial adapter cable. http://www.belkin.com/ search for Part Number: F5U409v1 2. Connect the 8-pin female end of the interface cable to the C3 Submersible Fluorometer’s 8-pin male connector. 3. Connect the interface cable’s 12V pin to the 12V power supply.

  • Page 11: Software Operation

    C3 Submersible Fluorometer to the PC date and time. Each channel (1-3) in the Settings Screen has a location associated with it on the C3 Submersible Fluorometer that is numerically labeled (1-3), respectively. Channels 4-6 are designated for the C6 Multi- Sensor Platform and are disabled for the C3.

  • Page 12: Current Data And Calibration Screens

    C3 Submersible Fluorometer 3.2.2 Current Data and Calibration Screen The Current Data Screen allows users to view real-time data acquisition, in both tabular or waveform display, as well as conduct calibrations for each channel. Current Data Channel names are displayed but cannot be changed in the Current Data screen.
  • Page 13 C3 Submersible Fluorometer Waveform Screen Users can graphically view real-time data for each sensor by clicking the “Display Waveform” box. Channel names and units will be displayed on the Y-axis. Time and date will be displayed on the X-axis. Clicking the “Autoscale” box will automatically scale graphs. Users may also rescale graphs by unchecking the “Autoscale”...
  • Page 14 C3 Submersible Fluorometer Calibration Screen Users can calibrate sensors in either the direct concentration mode, raw fluorescence mode, or blank subtracted raw fluorescence mode. Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted. The equation used...

  • Page 15: Direct Concentration Calibration

    Wait for the blank value to be measured and set for all 3 gains. Step 2: Standard Value and Units Place the C3 Submersible Fluorometer in a standard solution or use a secondary solid standard (see Appendix D for more information on solid standards).

  • Page 16: Raw Fluorescence Calibration - Blank Subtracted

    Relative Fluorescence Units (RFUB). See Appendix B for recommended practices. Step 1: Blanking Place the C3 Submersible Fluorometer in a blank solution. Note: A blank solution is a solution without the fluorophore of interest (i.e. de-ionized water, artificial water, or filtered water).

  • Page 17: Raw Fluorescence Calibration

    C3 Submersible Fluorometer 3.3.3 Raw Fluorescence Calibration In the Current Data screen select the “calibrate” button next to a specific channel. At the bottom of the Calibration Screen select, “click to use Uncalibrated Raw Fluorescence Mode”. Data for that channel will be uncalibrated and reported as “Relative Fluorescence Units”...

  • Page 18: Log Setup Screen

    C3 Submersible Fluorometer Atmospheric and Gravity Offset Step 1 (Method 2): Select the “Calibrate” button for depth in the “Current Data” screen. Pressure in PSI will be displayed in the “Current Pressure” window. After “Current Pressure” readings are stable, at desired location, select “Set Zero”.

  • Page 19: Enabling Data Output Ready State

    WARNING! As soon as data logging is enabled users will be unable to access C- Soft settings. This should be the last step before the C3 Submersible Fluorometer is deployed. 3.5 Enabling Data Output Ready State...

  • Page 20: Downloading And File Management

    (i.e. disconnected and reconnected) please allow 100 seconds for the data stream to begin. 3.6 Downloading and File Management Users can download new and existing files saved in the C3 Submersible Fluorometer memory. Select Download Data. Note: This step may take up to 10 minutes depending on file size.

  • Page 21: Maintenance And Warranty

    4.2 Warranty Terms Turner Designs warrants the C3 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following...

  • Page 22: Warranty Service

    To obtain service during the warranty period, the owner shall take the following steps: 1. Write, email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem. Phone: 1 (877) 316-8049 Email: support@turnerdesigns.com...
  • Page 23 C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste 998-2300 Rev. H Page 23...

  • Page 24: A C3 Submersible Fluorometer Specifications

    Appendix A: C3 Submersible Fluorometer Specifications C3 Submersible Fluorometer Weight in Air 1.64 kg (3.6 lbs) Length 23 cm (9.1 in.) Diameter 10 cm (3.9 in.) Material Delrin Temperature -2 to 50 ° C Temperature Accuracy 0.5 °C Temperature Resolution...

  • Page 25: B C3 Submersible Fluorometer Recommended Practices

    Identification Sensors are labeled 1, 2 or 3 adjacent to the optics on the face of the instrument. Each channel is factory set in the C3 Windows Software. Note: The channel labels can be modified in the C-Soft software. Recommended Measurement Practices...

  • Page 26: C C3 Submersible Fluorometer Wiring And Cable Guide

    The C3 Submersible Fluorometer outputs digital data in three formats: 1) Digital data that can only be read by C3 Submersible Fluorometer’s software, 2) Digital data in ASCII format that can be read by larger multi parameter systems such as CTD’s, and 3) Digital data that can be read and converted to analog data by the C-...
  • Page 27 The cabled configuration allows for real-time data transmission. The C3 measurements are sent from the RS-232 cable to a PC where the fluorescence data can be viewed and recorded on a computer or a data logger that can record digital ASCII data.

  • Page 28: D Linear Range, Quenching And Temperature Considerations

    Appendix D: Linear Range, Quenching and Temperature Considerations The linear range is the concentration range in which the C3 sensor output is directly proportional to the concentration of the signal. The linear range begins with the smallest detectable concentration and spans to an upper limit (concentration) that is dependent upon the properties of the material, filters used, and path length.

  • Page 29: E C-Fins Installation Guide

    ArcMap C3 Extension Steps: Prepare C3 Fluorometer Run C-Soft and set up C3 for log sampling interval of 1 second (see Log Setup Section 3.4 of C3 User’s Manual) Initiate datalog session as per instructions in Section 3.4 of manual. (C-Soft will...
  • Page 30 Connect to GPS on GPS toolbar (See ArcMap help for information on this) Set up C3 Connection using C3 Toolbar (Setup C3) Choose Com Port and Channel Settings then hit connect button. Data should start streaming in as shown below: Choose Shapefile to log to using Set Log File Button.
  • Page 31 Open Show Live C3 Data form which shows larger version of C3 data along with GPS data and Port/Logging Status. Start/stop logging to file using toolbar buttons Button Name Function Log Data To File Starts Logging to file based on Log interval...
  • Page 32 Flag value (0 = no flag ; -1 = Flagged) Flag_Notes Start Transect 1 Flag Notes Channel names and units are logged as per the C3 setup form and not C-soft. These can be changed during a logging session but it is not recommended. 998-2300 Rev. H...

  • Page 33: F Installing The Battery Bracket

    Allen Hex Key provided to tighten the Hex Screw located on the bracket’s locking ring around the battery. 2) Pull the base cap off of the C3 Submersible Fluorometer. 3) Position the Battery Bracket with the C3 so the alignment strips line up with one another and the bracket’s alignment pin fits into the...

  • Page 34: G Connecting The Submersible Battery Pack

    (P/N 2200-601) contains a open the battery housing. Please contact rechargeable lithium-ion battery and Turner Designs if you have any problems or battery pack charger. The battery questions related to the battery pack. pack is used together with the Internal...

  • Page 35: H Installing The Mechanical Wiper

    Tightening nut readings by reducing bio-fouling Replacement brushes available (P/N: 2300-460) Installation 1) Connect the C3 to a computer that has C-Soft software and supply 8) Click Test Wiper. power. 9) If the wiper does not come to rest 2) Start the C-Soft software.

  • Page 36: I Installing The Flow Cap

    2) protects sensors from damage during deployment or transport and 3) helps limit biofouling. The Flow Cap can be installed on C3 Submersible Fluorometers with or without the mechanical wiper. 2) Turning clockwise, hand tighten...
  • Page 37 3) Position the unit vertically with optical head facing upward (see diagram to the right). In this position the Flow Cap Housing can be used to hold standard solutions for calibrating the C3 sensors. Specifications Max. Pressure 100 psi Length 10.1 cm (3.98 in.)

  • Page 38: J Using The Secondary Solid Standard

    Match a specific sample Create a calibration correlation 2) Dry off the C3 Submersible Fluorometer’s optics, attach the Once a correlation is established Solid Standard Cap. between the Solid Standard and a...
  • Page 39 Screw Solid standards are not available for Turbidity or Ultraviolet sensors To change the signal level, insert Installation (C3 with wiper) the green screwdriver through the hole in the locking nut. Rotate it until it engages with the Remove the tightening nut and wiper adjustment screw that is beneath arm from the C3.

  • Page 40: K C-Series Analog Adapter

    C3 Submersible Fluorometer to a 0 - 5 VDC analog signal. The Adapter can convert the signal from 1, 2, or 3 channels installed on a C3. The voltage output is proportional to the fluorescence intensity measured by the sensors.
  • Page 41 Once calibration is complete, click the Log Setup tab to set the logging start time & logging interval. Click enable datalog, wait for C-Soft to automatically shutdown, and disconnect power. As soon as power is re-supplied to the C3 via the C-Series Analog Adapter it will automatically begin data output after 90 seconds, or pre- configured start time, at the set interval.
  • Page 42 Wiring Guide The wiring guide below indicates the function of each pin for the Analog Adapter’s 6- pin male end. To avoid damaging the Analog Adapter, please ensure the pins are only used for their intended purpose. Analog Adapter’s 6-pin male end Wire Color Function VBatt + (Positive)

  • Page 43: L How To Set Up Hyperterminal For Digital (Ascii) Data Capture

    5. Next, the HyperTerminal window will appear. You are now ready to connect the C3 Submersible Fluorometer to a power source. Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time.
  • Page 44 Most applications will integrate streaming data. Below are instructions on how to save streaming data on a PC. To save data: 1. On the HyperTerminal window toolbar, choose Transfer and then Capture Text. 2. When you click on Capture Text, a window will appear asking you to name and save the .txt file to the location of interest.

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