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Summary of Contents for Microtek ScanWizard Bio
- Page 1 Reference Manual...
- Page 2 Important Documents that you scan may be protected under copyright law. The unauthorized use of such documents could be a violation of the rights of the copyright holder. Microtek bears no responsibility for the unauthorized use of copyrighted materials. To obtain optimal results from the Microtek scanning software and user's manual, you should be familiar with such Windows concepts as pointing, clicking, dragging, and selecting from menus and dialog boxed.
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Page 3: Table Of Contents
Contents Introduction ................... 1 The ScanWizard Bio Interface ..............2 Launching the ScanWizard Bio ..............3 Exiting ScanWizard Bio ................3 The Preview Window ..............4 Elements of the Preview Window ............... 4 Preview Area ..................... 6 Scan Material ..................... 7 Unit of Measurement/Rulers ..............8... - Page 4 The Preferences Menu ................19 Scan Material ................... 19 Calibration Setup ................20 White/Black Points Setup ..............22 Cursor Auxiliary Lines ..............23 Overview Setup ................24 Prescan Setup ................... 26 Monitor Gamma Setup ..............27 Invert ....................27 More... command ................28 The Correction Menu ................
- Page 5 White/Black Points ................. 49 Tone Curve ..................... 52 Brightness and Contrast ................56 Exposure Time (for Transmissive mode only) ........... 57 Automatic Color Restoration ..............58 The Information Window ............59 Elements of the Information Window ............59 Zoom Level Display ................60 Mouse Cursor Position ................
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Page 7: Introduction
Introduction The ScanWizard Bio is a scanning software which is specifically designed for the use of the electrophoresis gel scanning. This manual covers the various commands and features found in the ScanWizard Bio scanning software for the Windows system. The manual information is divided into four major sections, corresponding to the four major windows of the program: •... -
Page 8: The Scanwizard Bio Interface
ScanWizard Bio consists of four major windows: Preview, Settings, Information, and Scan Job Queue. All four windows appear automatically after ScanWizard Bio is started up the first time. You may hide or show the Scan Job Queue and Information windows by clicking on the Hide/Show toggle commands in the View menu of the Preview window. -
Page 9: Launching The Scanwizard Bio
From Plug-in mode: Choose the Import or Acquire command from the File menu of your image-editing software, then select Microtek ScanWizard Bio. Exiting ScanWizard Bio To exit ScanWizard Bio, double click on the Close box on the upper left side of the Preview window. Click here to exit... -
Page 10: The Preview Window
The Preview Window The Preview window is the most prominent window of the four major windows, and it includes the various commands and tools for controlling the scanner. Elements of the Preview window Microtek ScanWizard Bio Reference Manual... - Page 11 Minimize button: Click it to minimize ScanWizard Bio. H. Exit button: Click it to close ScanWizard Bio. Take note that when you exit, you quit from ScanWizard Bio scanning software. Preview area: The overview image is displayed in this area after you perform a scan.
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Page 12: Preview Area
(or maximum scan area) . For example, if the scanner's maximum scan area is 14" x 17", the maximum size of the preview area will be limited under this dimension. Preview area To increase or decrease the preview window, drag the window to resize Microtek ScanWizard Bio Reference Manual... -
Page 13: Scan Material
If you are scanning positive films (such as electrophoresis gels or slides), this is the appearance of the Scan Material icon. When you click the icon and hold down the mouse, you will see the Transmissive option checked. Microtek ScanWizard Bio Reference Manual... -
Page 14: Unit Of Measurement/Rulers
To select the unit of measurement for the rulers: Click the unit box in the Settings window, or click the ruler unit button at the 0,0 point of the rulers in the Preview window. When the submenu appears, select the unit of measurement. Microtek ScanWizard Bio Reference Manual... -
Page 15: Overview, Prescan, And Scan To
Overview, Prescan, Scan to The Scan to button is one of ScanWizard Bio’ s great features, which functions the productivity tools under it. Click and hold the mouse button to activate Scan to options menu, you can select Scan to, Copy, or E-mail from the options provided. -
Page 16: Copy
Make sure that the check box “Send image to application after saving” has been checked. Then choose your preferred E-mail application from the options, and click Save. The saved file can now be attached to your E-mail message automatically. Microtek ScanWizard Bio Reference Manual... -
Page 17: Toolbar
You can use the Move tool for scrolling through zoomed-in images that were enlarged through the Zoom tool, or for scrolling through parts of an image not included completely within the frame of the preview window. Microtek ScanWizard Bio Reference Manual... -
Page 18: White/Black Points Pickers
As you pass over a point in the image, observe the Info window — the K values will be displayed in the Color Output Meter. These values are in turn based on the sample size you selected. Microtek ScanWizard Bio Reference Manual... -
Page 19: Auto Focus
Auto Focus is applied to. In this case, the software system automatically detects, calculates and selects the area applied with the Auto Focus function. The target sign marks the area applied with the Auto Focus function, which is selected by the software system. Microtek ScanWizard Bio Reference Manual... - Page 20 Preference menu and to select the More command, and then check the Auto Focus to activate this option. The Auto Focus function The Auto Focus is not activated. function is activated. Microtek ScanWizard Bio Reference Manual...
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Page 21: The Scanner Menu
The recommended replacement point is when the numbers on two sides of the slash symbol are equal. Press the Reset button to start recounting the scans for the roller after replacing a new one. Microtek ScanWizard Bio Reference Manual... -
Page 22: Scanner Control (Power Saving Control)
If your scanner does not show in the Scanner List dialog box, make sure your scanner is properly installed, connected, and turned on, and then click the Probe button. For details on connecting your scanner, refer to your scanner hardware installation guide. Microtek ScanWizard Bio Reference Manual... -
Page 23: The View Menu
Overview image and then clicking the Prescan button in the Preview window. Click the Prescan button. The Prescan image will then be available for viewing in the View menu, and a " " thumbnail will appear in the Scan Job Queue window. Microtek ScanWizard Bio Reference Manual... -
Page 24: Resize Window To Fit
Queue window, Information window, and Status bar on your screen. To use this feature, choose the correct command from the View menu for viewing a window. When the window appears, you can hide it by choosing the particular Hide command for it. Microtek ScanWizard Bio Reference Manual... -
Page 25: The Preferences Menu
The default scan material is Positive Film no matter what scanner model you are using. If you are scanning reflective materials, make sure that you switch to Reflective mode, or you will get inaccurate scanning results. Microtek ScanWizard Bio Reference Manual... -
Page 26: Calibration Setup
• Alternatively, you can click the Scan Material button and then choose the correct scan material from the drop-down menu that appears. Calibration Setup This command allows you to specify the calibration mode and reset the calibration data if needed. Microtek ScanWizard Bio Reference Manual... - Page 27 Calibration Modes • Smart: If this mode is checked, when you use the ScanWizard Bio for the first time, you will be asked to calibrate your scanner and the system will generate and store a set of calibration data with different values of scan types and resolutions.
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Page 28: White/Black Points Setup
The higher the percentage value, the lower the contrast will be. The Maximum Output Level lets you set the output level of the white point. The lower the percentage value, the lower the contrast will be. Microtek ScanWizard Bio Reference Manual... -
Page 29: Cursor Auxiliary Lines
As you draw the scan frame, cursor lines will appear to help you draw the scan frame precisely. When you release the mouse, your scan frame will be aligned with the cursor lines. Microtek ScanWizard Bio Reference Manual... -
Page 30: Overview Setup
For scanning reflectives By default, ScanWizard Bio overviews the maximum scan area as determined by your scanner model’ s bed size. You can, however, customize the overview area so that the scanner consistently overviews only the specific dimensions you have in mind. -
Page 31: Fast Overview
X-ray film (positive film) scanning. Preview button Click this button to get a scanning preview, or to do a new overview. This is helpful if you have specified custom settings or changed the dimensions. Microtek ScanWizard Bio Reference Manual... -
Page 32: Prescan Setup
Full screen, 75% screen, 50% screen, and Fit Preview Window. The size of the Prescan image does not necessarily fit into the specified option, as it is based only on the height/width ratio to get the maximum covered area. Microtek ScanWizard Bio Reference Manual... -
Page 33: Monitor Gamma Setup
In the case of 12-bit image, for example, a pixel in a positive image with a value of 255 is changed to 0, and a pixel with a value of 5 is changed to 250. Microtek ScanWizard Bio Reference Manual... -
Page 34: More
If checked, the image that is scanned in the Overview command remains on the screen until the next image is acquisition is performed. If unchecked, the overview image is deleted when you exit ScanWizard Bio. Keep All Prescan Images If checked, the image that is scanned in the Prescan command remains on the screen until the next image is acquisition is performed. -
Page 35: Scan Quality
(i.e., 10-, 12-bit, or higher), and then converted to the desired output depth. This special operation results in the best image quality possible. Microtek ScanWizard Bio Reference Manual... -
Page 36: Working Directory
This lets you specify a place to store temporary working files (e.g., scan job files) during a scanning session. If the directory you specify is not found or does not exist, a warning message appears, and ScanWizard Bio will create one automatically for you. -
Page 37: The Correction Menu
For more information, see the section of the manual on Advanced Image Correction The Help Menu The Help menu lets you access on-line help for ScanWizard Bio, and gives you information on the ScanWizard Bio scanning software. About This command displays the ScanWizard Bio splash screen and shows the program’... -
Page 38: The Settings Window
The Settings window contains the parameters for outputting your scanned image for the current scan job and includes the advanced image correction tools of the program. Elements of the Settings window Clicking the Window Expansion button to expand the Settings window. Microtek ScanWizard Bio Reference Manual... - Page 39 Unit of measurement: This lets you chose your desired unit of measurement, which will then be reflected in the rulers along side the Preview window. Choose from inch, cm, mm, point, pica, or pixel. Microtek ScanWizard Bio Reference Manual...
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Page 40: Scan Job
Scan Job Queue window can show you which is your currently selected scan job. Correspondence between the Scan Job box in the Settings window and the current scan job in the Preview and Scan Job Queue windows Microtek ScanWizard Bio Reference Manual... -
Page 41: Image Type
The Type menu determines what your resulting scan will be. It does not refer to the original image type. ScanWizard Bio allows a direct scanning in either of RGB Colors, RGB Colors (48-bit), Gray Scale, or Gray Scale (16-bit) color spaces. -
Page 42: Resolution Settings
To choose your resolution, click the arrow button next to the Resolution box, and choose your setting from the list box. Microtek ScanWizard Bio Reference Manual... -
Page 43: Resolution Unit
Conversely, if the resolution is too high, the file size becomes unwieldy, and y our file ends up containing more information than the printer needs, slowing down the file transfer and the printing process. Microtek ScanWizard Bio Reference Manual... -
Page 44: Scan Frame And Related Settings
9” x 7” original to be output to 4.5” x 3.5”). Increase the scaling if outputting at a larger size. To choose the scaling percentage, click the arrow button next to the Scaling box, and choose your setting from the list box. Microtek ScanWizard Bio Reference Manual... -
Page 45: Output Settings
To choose the ruler unit, click the arrow button next to the box, and choose your setting from the list box. Important: Make sure you select the correct unit of measurement before entering any of the values for width or height in the Scan Frame Settings or Output Settings. Microtek ScanWizard Bio Reference Manual... -
Page 46: Multiple Sampling
Select the image area in the preview image where multiple sampling will be applied. In the Settings window, click the “M. Sampling” drop-down menu, and choose an option. Click the “Scan” button to start scanning. Multiple sampling is applied, producing a higher-quality image. Microtek ScanWizard Bio Reference Manual... -
Page 47: Transform
The Reset button in the Settings window brings up a dialog box where you can specify the AIC settings to be reset to their default values. To reset any or a combination of settings, check the boxes next to the targeted settings. Microtek ScanWizard Bio Reference Manual... -
Page 48: Advanced Image Correction Tools
Bio. With the AIC tools, you can adjust or enhance images right from within ScanWizard Bio and preview the changes to your image even before you scan. The various AIC tools available for use will be shown depending on the Scan Material chosen in the Preview window. -
Page 49: Accessing The Aic Tools
To access an AIC tool, click a particular tool in the Settings window or choose its counterpart in the Correction menu in the Preview window. After clicking a tool, the AIC dialog box appears. The elements of the AIC dialog box are explained in the next section. Microtek ScanWizard Bio Reference Manual... -
Page 50: Elements Of Aic Dialog Box
“up” arrow at the left side of the dialog box. E. Preview option If checked, changes are applied immediately to the Preview window image. If unchecked, changes are seen only after you close the AIC dialog box. Microtek ScanWizard Bio Reference Manual... - Page 51 J. Arrow button: This allows you hide/show the thumbnails. K. Core of the AIC screen This is the heart of the AIC screen, and the content here changes to reflect your selected AIC tool. Microtek ScanWizard Bio Reference Manual...
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Page 52: The Action Buttons
This button to cancel out the changes that were made with the current image correction tool. This means that if you used several AIC tools, Revert cancels the effect of only the last used (or current) tool, and preserves the effects of the other preceding tools. Microtek ScanWizard Bio Reference Manual... -
Page 53: Custom Settings
Add To Menu ... button in that tool's dialog box. When a dialog box comes up, enter the name of the new custom setting to be added. For instance, the added setting can be called “test 1” as shown below. Microtek ScanWizard Bio Reference Manual... -
Page 54: Removing Custom Settings
To load or retrieve a custom setting that you have previously defined for an AIC tool, go to that AIC tool’ s pop-up menu, and choose the setting you wish to be loaded. Custom setting to be retrieved or loaded Microtek ScanWizard Bio Reference Manual... -
Page 55: White/Black Points
B. Sliders The sliders are used to adjust the Black and White points of the image. The values of the black and white points are reflected in the edit boxes below. Microtek ScanWizard Bio Reference Manual... - Page 56 To set the White point: Click the Picker on the right, then click on the preview image to define the reference point. • To set the Black point: Click the Picker on the left, then click on the preview image to define the reference point. Microtek ScanWizard Bio Reference Manual...
- Page 57 Another alternative is to simply click the Auto button, so that the shadow and highlight for the image is determined automatically. When the changes are done, click OK. For more details on the effects of the other action buttons, see the section “The Action Buttons”. Microtek ScanWizard Bio Reference Manual...
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Page 58: Tone Curve
Input and Output values are the same. When the curve is moved up or down, the relationship between input value and output value changes accordingly. Microtek ScanWizard Bio Reference Manual... - Page 59 The Method sets the kind of curve you wish to have. Select from Line, Curve, or Gamma. C. Channel The Channel allows you to choose the gray channel only in which the gamma will be affected. Microtek ScanWizard Bio Reference Manual...
- Page 60 G. The Load button The Load button lets you load a previously saved tone curve for use with another image. Photoshop-saved curves can also be loaded. Microtek ScanWizard Bio Reference Manual...
- Page 61 For more details on the effects of the other action buttons, see the section “The Action Buttons”. To save a curve, click the Save As button. To load a previously saved curve, click the Load button and specify the curve setting to be loaded. Microtek ScanWizard Bio Reference Manual...
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Page 62: Brightness And Contrast
Contrast: The Contrast control lets you change the contrast setting. Too much contrast will make an image look like a photocopy of a picture, with little or no gray shades left. Too little contrast will make the colors in the image look dull and flat. Microtek ScanWizard Bio Reference Manual... -
Page 63: Exposure Time (For Transmissive Mode Only)
A high exposure value will extend scanning processing time, for it requires extra time to produce a better visible image. Note: The Exposure Time function is available only in the Settings window when the connected scanner is supported by this function (e.g., Bio-5000). Microtek ScanWizard Bio Reference Manual... -
Page 64: Automatic Color Restoration
In the Settings window, check the “Automatic Color Restoration” option. Click the “Scan To” button to perform scanning. Automatic Color Restoration is applied, and faded colors in the photo are restored. Microtek ScanWizard Bio Reference Manual... -
Page 65: The Information Window
Preview window (or the thumbnail in the Advanced Image Correction dialog box if it is open). You can choose to show or hide the Information window in ScanWizard Bio. To do this, click the Show/Hide Info window toggle command under the View menu in the Preview window. -
Page 66: Zoom Level Display
Knowing this, you can modify the shadow and highlight points of an image, then come back to the same point in the image, and verify through the Color Output Meter that the K values have indeed changed. Microtek ScanWizard Bio Reference Manual... -
Page 67: Sample Display Area
5-pixel by 5-pixel area. If you choose 1 x 1 (the default), the color information pertains to a single pixel — the pixel in the middle of the sample area. Microtek ScanWizard Bio Reference Manual... -
Page 68: The Scan Job Queue Window
Scan Job Queue window. You can choose to show or hide the Scan Job Queue window in ScanWizard Bio. To do this, click the Show/Hide Scan Job Queue window toggle command under the View menu in the Preview window. - Page 69 ) appears for the selected scan job after you click the Prescan button in the Preview window. This means a prescan image is available for that scan job. This function is available only in the Reflective mode. Microtek ScanWizard Bio Reference Manual...
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Page 70: One-Pass Scan For Multiple Scan Jobs
If the screen filter is applied, all the scan jobs should use the same descreen lpi value. If ScanWizard Bio has detected that your image original does not meet the above conditions, the “One-pass Scan for Multiple Jobs” option is dimmed and will not be available for selection. -
Page 71: Multiple Auto-Crop For Ez-Lock Film Holder
Scan Job Queue window, numbered sequentially and all marked by a “Check” that indicates the jobs are ready to be scanned. Note: After performing multiple auto-crop scanning, the previously created jobs will be removed from the Scan Job Queue window. Microtek ScanWizard Bio Reference Manual... -
Page 72: Function Buttons
Editing Multiple Scan Jobs ScanWizard Bio lets you edit multiple scan jobs at the same time, increasing your efficiency in using the software. To edit multiple scan jobs, simply select all the scan jobs to be edited in the Scan Job Queue window. -
Page 73: Adding A New Scan Job
Removing a Scan Job The Delete button allows you to remove a scan job from the list of scan jobs available. To delete a scan job, highlight the scan job to be removed, then click the Delete button. Microtek ScanWizard Bio Reference Manual... -
Page 74: Checking A Scan Job
When you start scanning, the scan jobs will be processed and scanned in the order that they appear in the Scan Job Queue window. Microtek ScanWizard Bio Reference Manual... -
Page 75: The Load/Save Button
Before loading a new scan job, you may remove your existing scan jobs from the right column Click the Add>> button in the window to load the scan jobs from a chosen folder into the Scan Job Queue window. Microtek ScanWizard Bio Reference Manual... - Page 76 Suppose the scan jobs already exist, if you check this check box, the number suffix is automatically appended as a new scan job. If unchecked, the existing scan jobs are overwritten. Microtek ScanWizard Bio Reference Manual...
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Page 77: Appendix
• The scanner's serial number. This can be found on the back panel of the scanner. • Your computer name and model. • The version number of ScanWizard Bio. This is indicated on the ScanWizard Bio scanning software. • Your system components, or the devices on your system, such as an external hard drive, CD-ROM, etc.