Appendix 4. Non-radioactive
labeling and detection systems
Labelling and detection
system
AlkPhos Direct (RPN3690)
ECL Direct (RPN3000)
RPN2510EUM Appendix 4 Rev C 06/2007
Sensitivity
Time from
hybridization
to detection
0.06 pg
1 hour
0.5 pg
1 hour
Duration of
Strip before
light output
re-probing
5 days
yes
1–2 hours
no
17
Quantification Recommended application
no
Single copy Southern and
Northerns
no
High target applications
eg. colony/plaques