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Summary of Contents for GE RPN2512E
- Page 1 GE Healthcare Amersham Hybridization oven/shaker Product User Manual Codes: RPN2510E RPN2511E RPN2512E...
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Page 2: Table Of Contents
Page finder 1. Legal 2. Introduction 3. Safety warnings and precautions 3.1. Electrical Installation 4. Specification 5. Setting up the Hybridization Oven/Shaker 5.1. Setting the oven temperature 5.2. Setting up the rotisserie 5.3. Setting up the platform shaker 6. Hybridization using the platform shaker 7. -
Page 3: Legal
1. Legal GE, imagination at work and GE monogram are trademarks of General Electric Company. Amersham, Alkphos Direct, ECF, ECL, ECL Plus, ECL Advance, Hybond, Hypercassette, Hyperfilm, Hyperscreen, Hypertorch, Megaprime, Nick, Rapid- hyb, Ready-To-Go, Rediprime, Sensitive and Trackertape are trademarks of GE Healthcare companies. -
Page 4: Introduction
Using the GE Healthcare Hybridization Oven/Shaker ensures that the temperature and shaking/rotation frequency, and hence the stringency of hybridization and washing steps are rigidly controlled. This enables rapid and reproducible probing of nucleic acids, and proteins immobilized on nylon and nitrocellulose membranes. -
Page 5: Safety Warnings And Precautions
IMPORTANT: The Hybridization Oven/Shaker has been supplied with a cordset and plug. Check that the appropriate plug for your electrical supply has been included. If there is any doubt consult a qualified electrician and inform your local GE Healthcare sales office. -
Page 6: Specification
Product code 230 V / 50 Hz / 3.1 A RPN2510E 110/120 V / 60 Hz / 4 A RPN2511E 100 V / 50/60 Hz / 4 A RPN2512E Each instrument is supplied with 1 rotisserie (RPN2514), 6 hybridization bottles (RPN2516) and an instruction manual. Accessories Rotisserie, holds 7 × 40 mm hybridization bottles RPN2514 Rotisserie, holds 2 × 75 mm and 2 × 40 mm hybridization bottles RPN2515 Hybridization bottle, 260 ×... -
Page 7: Setting Up The Hybridization Oven/Shaker
Hybridization Oven/Shaker is found on the product information label on the rear of the instrument. 5. Turn the Mains ON/OFF switch (1 on Fig 1) to the ON position. 6. The GE Healthcare Hybridization Oven/Shaker is now ready for use. Rotisserie Temperatur e (... -
Page 8: Setting Up The Platform Shaker
4. Place the rotisserie into the oven onto the rotation mechanism, ensuring that the serrated bands at either end of the rotisserie locate onto the steel cogs of the rotation mechanism at the rear of the oven. (B on figure 2). The plastic flanges of the rotisserie locate on to the small wheels on the oven floor. -
Page 9: Hybridization Using The Platform Shaker
The Hybridization Oven/Shaker is compatible with the hybridization technologies available from GE Healthcare. These include radioactive hybridizations using Rapid hyb buffer and the range of non-radioactive systems for the labelling and detection of proteins and nucleic acids (see appendix 1). -
Page 10: Hybridization Using The Rotisserie
However, studies at GE Healthcare laboratories using a wide range of hybridization recommended. mesh technologies demonstrate a resulting loss of sensitivity due to partial absorption of the probe into the mesh. -
Page 11: Membrane Washing Procedures
4. Prehybridize the membrane for the specified length of time at a rotisserie speed of 4 rpm. 5. Following prehybridization, turn off the rotisserie, remove the rotisserie from the oven. Add the labeled probe to the buffer, either by removing an aliquot of the buffer, adding the probe and returning the aliquot to the bottle;... -
Page 12: Maintenance/Care/Cleaning Of The Oven/Shaker
4. Never clean unit with abrasive pads or cleaners. 5. Never clean unit with acetone or chloroform. Only spare parts supplied or specified by GE Healthcare or its agent should be used. Fitting of non-approved parts may affect the performance of the safety features designed into the instrument. -
Page 13: Troubleshooting Guide
9. Troubleshooting guide This section briefly summarizes some of the potential problems encountered during membrane hybridizations. More complete troubleshooting guides are supplied in the pack leaflet that accompany each product. Symptom Cause Remedy 1. Membrane curling up in 1.1. Incorrect orientation of bottle in 1.1. -
Page 14: Appendix 1. Products For Electrophoresis
Appendix 1. Products for electrophoresis DNA Markers Precise Sizing Digest of Natural DNAs 50 Base-pair 100 Base-pair KiloBase DRigest III ladder ladder DNA marker 27-4060-01 27-4005-01 27-4007-01 27-4004-01 2000 10000 23130 1900 8000 9416 1800 6000 6557 1700 5000 4361 1600 4000 2322... -
Page 15: Appendix 2. Hybond Membranes For Nucleic Acid Applications
Appendix 2. Hybond membranes for nucleic acid applications Size Pack size Hybond N+ Hybond XL Hybond N Hybond NX Hybond ECL Hybond C Extra Hybond P 82 mm 50 discs RPN82B RPN82S RPN82N RPN82T RPN82D RPN82E 87 mm 50 discs RPN87B RPN87S RPN87N... -
Page 16: Appendix 3. Radioactive Labeling Systems
Appendix 3. Radioactive labeling systems Labelling Technology Nucleotide Amount of Labelling Probe specific Recommended Product system template time activity application code (dpm/µg) Rediprime™ II Random-prime dCTP only 25 ng 10 minutes 2 × 10 membrane RPN1633 hybridization RPN1634 Ready-To-Go™ Random-prime dCTP only 10 ng–1 µg 5 minutes... -
Page 17: Appendix 4. Non-Radioactive Labeling And Detection Systems
Appendix 4. Non-radioactive labeling and detection systems Labelling and detection Sensitivity Time from Duration of Strip before Quantification Recommended application system hybridization light output re-probing to detection AlkPhos Direct (RPN3690) 0.06 pg 1 hour 5 days Single copy Southern and Northerns ECL Direct (RPN3000) 0.5 pg... -
Page 18: Appendix 5. Products For Autoradiography And Chemiluminscent Detection
Appendix 5. Products for autoradiography and chemiluminscent detection Hyperfilm – high performance autoradiography films Product description size number sheets code Hyperfilm ECL 5 × 7 inches 28-9068-35 Hyperfilm ECL 18 × 24 cm 28-9068-36 Hyperfilm ECL 18 × 24 cm 28-9068-37 Hyperfilm ECL 8 ×... -
Page 19: Appendix 6. Hybridization Buffer
Appendix 6. Hybridization buffer Product name Pack size Code Rapid-Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM Appendix 6 Rev C 06/2007... -
Page 20: Appendix 7. Radiation Safety Products
Appendix 7: Radiation safety products Product Description Product Dimensions Quantity Code Number CDC storage box 240 × 140 × 130 mm 1 box RPN2032 Pipette guards Beta pipette guard, Gilson P20/100 1 guard RPN1544 Beta pipette guard, Gilson P200 1 guard RPN1545 Beta pipette guard, Gilson P1000 1 guard... - Page 22 GE Healthcare GE Healthcare offices: GE Healthcare Bio-Sciences AB Björkgatan 30, 751 84 Uppsala, Sweden GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freiburg, Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, GE Healthcare Bio-Sciences KK Sanken Bldg.
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