Table of Contents
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Table of Contents
Related Manuals for Biotage Flash 150
Summarization of Contents
Biotage Flash 75/150 User Manual
User Manual
A guide for operating the Biotage Flash 75/150 system.
System Components
Prepacked Biotage® Flash Cartridge
Details about the prepacked flash cartridges offered by Biotage.
Radial Compression Module
Description of the radial compression module, a key system component.
Head Assemblies
Describes the top and bottom head assemblies for sealing the cartridge.
AM-190 Manifold
Explains the function and features of the AM-190 manifold.
Solvent Reservoir
Details the solvent reservoir options and features.
Sample Injection Module (Biotage® SIM) Kit
Information on the SIM kit for sample loading.
Sanitary Gaskets
Discusses different types of sanitary gaskets and their compatibility.
Grounding Kit
Explains the grounding kit for static discharge prevention.
Start-Up Kit
Lists the components included in the start-up kit.
Cartridge Extraction Tool
Describes the tool used for removing cartridges.
Chemical Resistance
Lists chemicals compatible with system wetted parts.
Safety
Intended Use
Defines the proper usage and operational environment for the system.
Education, Training, and Competence
Outlines personnel requirements for safe operation and maintenance.
Warranty and Liability
Refers to terms and conditions for warranty and liability.
Service
Information regarding system service and maintenance procedures.
Labels
Details the various labels found on the system.
Safety Requirements
Essential safety precautions for installation and operation.
Installation
Overview of the installation process.
Operation
Guidelines for safe operation of the system.
Installation
Set Up the AM-190 Manifold
Step-by-step guide for setting up the AM-190 manifold.
Set Up the Radial Compression Module
Instructions for setting up the radial compression module.
Set Up the Sample Injection Module (Biotage® SIM)
Procedures for setting up the optional Sample Injection Module (SIM).
Set Up the Solvent Reservoir
Steps for setting up the solvent reservoir.
Attach the Inert Gas Supply and Solvent Lines
Guidance on connecting gas and solvent lines.
How to Use the System
Fill the Solvent Reservoir
Instructions on how to fill the solvent reservoir.
Insert a Flash Cartridge
Steps for inserting a flash cartridge into the module.
Pressurize the Radial Compression Module and the Solvent Reservoir
Procedures for pressurizing the system components.
Equilibrate the Cartridge
How to equilibrate the chromatography cartridge.
Load Sample and Collect Fractions
Guidance on loading samples and collecting fractions.
Refill the Solvent Reservoir
Steps to refill the solvent reservoir during operation.
Empty the Solvent Reservoir
Procedure for emptying the solvent reservoir.
Depressurize the Radial Compression Module and Remove the Used Cartridge
Steps to depressurize and remove a used cartridge.
Clean the Sample Injection Module (Biotage® SIM)
Instructions for cleaning the SIM module.
Troubleshooting
AM-190 Manifold
Troubleshooting common issues with the AM-190 manifold.
Biotage® Flash 75/150 Radial Compression Module
Troubleshooting issues related to the radial compression module.
Operation
Troubleshooting common operational problems.
Sample Injection Module (Biotage® SIM)
Troubleshooting SIM solvent compatibility issues.
Solvent Reservoir
Troubleshooting common problems with the solvent reservoir.
Optimizing Flash Separations
Column Separations are Governed by ACV, Not ARf
Explains how column separation is governed by ACV, not ARf.
Vary Solvent Strength to Maintain Rf Values Between 0.15 and 0.40
How to adjust solvent strength for optimal Rf values.
Vary Solvent Selectivity to Maintain Rf Values Between 0.15 and 0.40
How to adjust solvent selectivity for optimal Rf values.
The Relationship Between the Size of a Column and the Sample Load is Dependent on ACV
How column size and sample load relate to ACV.
General Information
Price List and Ordering Information
Information on obtaining prices and ordering.
Download User Documentation
How to download additional documentation.
Manufacturer
Information about the manufacturer.
Contact Us
Contact details for Biotage.
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