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Dionex Manuals
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DNAPAC PA200
Product manual
Dionex DNAPAC PA200 Product Manual
Analytical column, guard column
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Table of Contents
Table of Contents
Section 1 - Introduction
Dnapac PA200
Biolc System (Without Columns)
Selectivity in Sodium Perchlorate (Naclo 4 ) Gradients
Guard Column Use
Dnapac Pa200 Anion Exchange Columns
Dnapac Column Family
Section 2 - Operation and System Requirements
System Requirements
System Operation Requirements
Dnapac Pa200 Column Operational Parameters
Section 3 - Purity Requirements for Chemicals
Deionized Water
Inorganic Chemicals
Solvents
Section 4 - Quality Assurance
Certificate of Performance - Resin Batch Testing
Production Test Chromatograms
Section 5 - Methods Development
Sample Cleanup
Elution Order
Effect of Salt Type on Oligonucleotide Elution
Eluent Strength
Loading Capacity
Gradient Slope
Effect of P Hand Solvent on Oligonucleotide Chromatography
Effect of Ph on Hydrogen Bond Interactions
Effect of Ph on Retention
Effect of Solvent on Retention
Effect of Ph on Selectivity
Effect of Temperature on Oligonucleotide Retention
Effect of Terminal Base on Selectivity
Selectivity in Sodium Chloride (Nacl) Gradients
Application-Specific Mobile Phase Recommendations
For Synthetic Odns Where the Goal Is to Evaluate Purity
When Multiple Possible Odns of Similar Length in the same Solution Must be Resolved
Section 6 - Applications
Denaturing Conditions for Control of Secondary Structure
Effect of High Temperature and High P Hon Column Lifetime
Phosphodiester Analysis
Sodium Perchlorate Eluent Systems
Sodium Chloride Eluent Systems
SECTION 7 - Dnapac™ PA200 RESOURCES
Document no
Esolution
No Peaks, Poor Peak Area Reproducibility or Unexpectedly Small Peak Area
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Dnapac Pa200 Anion Exchange Columns
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No Peaks, Poor Peak Area Reproducibility or Unexpectedly Small Peak Area
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DNAPac PA200
Document No. 065036
Page 1 of 25
PRODUCT MANUAL
DNAPAC PA200 ANALYTICAL COLUMN
(4 x 250mm, P/N 063000)
DNAPAC PA200 GUARD COLUMN
(4 x 50mm, P/N 062998)
©DIONEX Corporation
Document No. 065036
Revision 01
20 August 2004
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