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DNAPAC PA200
User Manuals: Dionex DNAPAC PA200 HPLC Columns
Manuals and User Guides for Dionex DNAPAC PA200 HPLC Columns. We have
1
Dionex DNAPAC PA200 HPLC Columns manual available for free PDF download: Product Manual
Dionex DNAPAC PA200 Product Manual (25 pages)
ANALYTICAL COLUMN, GUARD COLUMN
Brand:
Dionex
| Category:
Analytical Instruments
| Size: 0 MB
Table of Contents
Table of Contents
2
Section 1 - Introduction
4
Dnapac PA200
4
Biolc System (Without Columns)
4
Selectivity in Sodium Perchlorate (Naclo 4 ) Gradients
4
Guard Column Use
5
Dnapac Pa200 Anion Exchange Columns
5
Dnapac Column Family
5
Section 2 - Operation and System Requirements
6
System Requirements
6
System Operation Requirements
6
Dnapac Pa200 Column Operational Parameters
7
Section 3 - Purity Requirements for Chemicals
8
Deionized Water
8
Inorganic Chemicals
8
Solvents
8
Section 4 - Quality Assurance
9
Certificate of Performance - Resin Batch Testing
9
Production Test Chromatograms
10
Section 5 - Methods Development
11
Sample Cleanup
11
Elution Order
11
Effect of Salt Type on Oligonucleotide Elution
11
Eluent Strength
11
Loading Capacity
11
Gradient Slope
12
Effect of P Hand Solvent on Oligonucleotide Chromatography
12
Effect of Ph on Hydrogen Bond Interactions
12
Effect of Ph on Retention
13
Effect of Solvent on Retention
13
Effect of Ph on Selectivity
14
Effect of Temperature on Oligonucleotide Retention
15
Effect of Terminal Base on Selectivity
16
Selectivity in Sodium Chloride (Nacl) Gradients
16
Application-Specific Mobile Phase Recommendations
18
For Synthetic Odns Where the Goal Is to Evaluate Purity
18
When Multiple Possible Odns of Similar Length in the same Solution Must be Resolved
18
Section 6 - Applications
19
Denaturing Conditions for Control of Secondary Structure
19
Effect of High Temperature and High P Hon Column Lifetime
19
Phosphodiester Analysis
20
Sodium Perchlorate Eluent Systems
20
Sodium Chloride Eluent Systems
21
SECTION 7 - Dnapac™ PA200 RESOURCES
22
Document no
23
Esolution
24
No Peaks, Poor Peak Area Reproducibility or Unexpectedly Small Peak Area
25
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