Related Manuals for ELITechGroup CYTOPRO AC-160
Summary of Contents for ELITechGroup CYTOPRO AC-160
- Page 1 CYTOPRO ® CYTOCENTRIFUGE ROTOR Model AC-160 Applications Manual For Aerospray® Models 7xx2 57-2007-01C...
- Page 2 ©2013 ELITechGroup Inc. All rights reserved. Printed in the United States of America. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means whatsoever, without the prior express written permission of ELITechGroup Inc.
-
Page 3: Table Of Contents
Table of Contents Section 1 Introduction 1.1 Cytopro Cytocentrifuge Rotor Overview ........5 Using this Manual ..............5 Functional Description ............6 Key Features ................. 6 Intended Use ................ 6 Table 1: Cytopro Rotor Specifications ........7 Table 2: Sample Treatment Options ........8 Table 3: Explanation of Symbols ........... - Page 4 Table of Contents Section 3 Operating the Cytopro Cytocentrifuge (continued) Single or Dual Chambers..........23 Cytopro Magnum Chambers......... 23 Loading Samples ..............24 Performing a Cytocentrifuge Cycle ........24 Unloading the Rotor ............. 25 Separating Chambers from Cytopads ........26 Attaching Chambers to Cytopads .........
-
Page 5: Introduction
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Using this Manual This manual provides instructions to install, operate, and maintain the Cytopro Cytocentrifuge Rotor. The manual is an important part of the product. Read it carefully and completely before setup and first use of the instrument. If additional accident prevention and environmental protection requirements exist in the country of operation, this manual must be supplemented by appropriate instructions to ensure compliance. -
Page 6: Functional Description
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview WARNING! If power is lost during cytocentrifugation, the lid remains locked until power is restored. Do not attempt to open the lid while power is off. CAUTION: Use only spare parts supplied or specified by Elitech Group. Using non-approved parts may affect the performance and safety features of the instrument. - Page 7 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Table 1: Cytopro Rotor Specifications Category Characteristics Sample Well Capacity* Single: 0.5 mL max Dual: 2 x 0.3 mL Cytopro Magnum: 6 mL Cell Deposit Area Single = 38.5 mm (7 mm diameter) Dual = 77 mm (2 x 7 mm diameter) Cytopro Magnum = 315 mm...
- Page 8 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Table 2: Sample Treatment Options The chart below suggests procedures for various fluids. Refer to the Methods Manual for more detailed information. Methods currently used in other cytocentrifuges will often work in the Cytopro, if the maximum volume of fluid and the run time is adjusted appropriately (see chart).
- Page 9 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Table 3: Explanation of Symbols Biological Hazards (Biological Risks) Alternating Current (AC) Power ON Power OFF Caution, Consult Accompanying Documents (Attention, see instructions for use) Biological Hazard Symbol Manufacturer’s Representative in Europe Manufactured by Waste Electrical and Electronic Equipment (WEEE) CE Mark, Product meets the essential requirements designated in Annex I of the...
-
Page 10: The Cytopro Rotor
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview The Cytopro Rotor The Cytopro rotor holds up to eight sample chamber assemblies, and microscope slides. The rotor operates on the drive hub of the stainer. The self-sealing, autoclavable rotor is easy to load in a biological safety cabinet. -
Page 11: Single Sample Chamber
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Figure 2: Front Panel and Touchscreen (Gram Stainer shown) 1 – Standby/Ready Button 2 – Touch Screen The front panel features an interactive touchscreen display. Refer to Touchscreen and User Interface (Section 1.2, Table 4) for more information. Single Sample Chamber The reusable single chamber features a dual-port sample loading port system that places a 38.5 mm... -
Page 12: Dual Sample Chambers
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Figure 3: Single Chamber Assembly 1 – Chamber Frame 2 – Cytopad 3 – Chamber Base 4 – Tunnel Port Cap Vent 5 – Sample Well Cap Vent 6 – Cap 7 – Sample Well 8 –... -
Page 13: Cytopro Magnum Sample Chambers
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Figure 4: Dual Sample Chamber 1 – Chamber Frame 2 – Cytopad 3 – Chamber Base 4 – Cap Vents 5 – Cap 6 – Sample Wells 7 – Chamber Pressure Rings 8 –... -
Page 14: Cytopro Absorption Pads
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Figure 5: Cytopro Magnum Chamber 1 – Cytopro Magnum Chamber 2 – Chamber Cap 3 – Suspension Fluid Absorption Chambers (2) 4 – Sealing Gasket Cytopad Absorption Pads Cytopads (standard chambers only) absorb suspension fluid and allow sample cells to sediment onto the microscope slide. -
Page 15: Chamber Frame
SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Rotor Overview Chamber Frame Chamber frames accept either single or dual replacement pads and have a cutaway to prevent cells from being smeared as the chamber assembly is removed. Indexing pins on the frame ensure correct Cytopad alignment. -
Page 16: Touchscreen And User Interface
SECTION 1 INTRODUCTION 1.2 Touchscreen and User Interface Users control all instrument functions from the interactive touchscreen display. Only those functions that pertain to cytocentrifugation are discussed in this manual. See the Aerospray Stainer User’s manual for more information. Table 4: Front Panel/Main Screen Function Keys Button Name Description... - Page 17 SECTION 1 INTRODUCTION 1.2 Touchscreen and User Interface Table 4: Front Panel/Main Screen Function Keys (continued) Button Name Description Cyto Programs Allows users to create, edit, and delete cytocentrifuge programs QC/Maintenance Enables slide tracking, preventive maintenance Tracking tracking, and reagent tracking System Log Allows users to control logging functions Save...
-
Page 18: Controlling And Customizing Cytocentrifuge Functions
SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Many software settings can be controlled from the System Setup menu. Only functions related to cytocentrifugation are explained in this manual. Refer to the Aerospray Stainer User manual for complete information. -
Page 19: Editing A Cytocentrifuge Program
SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Editing a Cytocentrifuge Program From System Setup, press Cyto Programs. Select the program to be modified. Press Edit. Adjust the settings as desired. Press Save. Changing the Program Name: From Adjust Settings menu, select Program Name. -
Page 20: Recording Specimen And Slide Information
SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.2 Recording Specimen and Slide Information Scanning Slides with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Cyto Slide Tracking. NOTE: Selecting Enable Cyto Slide Tracking changes the Start button on the Main menu to ”Load Slides.”... -
Page 21: Manually Entering Specimen Information
SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.2 Recording Specimen and Slide Information Manually Entering Specimen Information With Stain Slide Tracking and Manual Entry enabled in the QC Maintenance menu: Press Load Slides on the Main Menu. Press Add to reveal the keypad. Enter slide information (maximum of 24 characters) and/or sample information and press Return. -
Page 22: Operating The Cytopro Cytocentrifuge
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Suggested Cytocentrifugation Protocol If enabled, scan or enter cyto slide and/or sample information. • Prepare and load slides into the rotor. • • Load chambers into the rotor. Load samples into the chambers. •... -
Page 23: Loading Chambers Into The Rotor
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Preparing and Loading Slides (continued) If Slide Tracking is enabled, press Load Slides. • If using the barcode reader, scan each specimen slide and sample barcode before loading it into the carousel. Slide tracking must be enabled from the System Setup menu. -
Page 24: Loading Samples
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Loading Samples Load sample and prewetting fluids through cap vents, or directly into the ports before the cap is placed on the chamber. Use the chamber caps to minimize contamination and accidents. Chamber caps are mandatory for the Cytopro Magnum. -
Page 25: Unloading The Rotor
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle The display shows the progress of the program, and a signal tone (if enabled) indicates the end of the cycle. NOTE: Use the emergency Stop button when required, for example, if there is abnormal vibration or noise. -
Page 26: Separating Chambers From Cytopads
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Unloading the Rotor (continued) Cytopro Magnum chambers must remain in the rotor and in contact with the slide for 45 seconds after cytocentrifugation has stopped. This allows the fluid to be completely absorbed by the absorbent media. -
Page 27: Attaching Chambers To Cytopads
SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Attaching Cytopads to Chambers To reuse a chamber, attach a new Cytopad after the chamber is cleaned, disinfected, and thoroughly dried. To assemble Cytopads with sample chambers: Place a Cytopad inside a chamber frame, using the indexing pins for correct positioning. -
Page 28: Preventive Maintenance And Safety
SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.1 Routine and Preventive Maintenance The Cytopro is designed to be simple to use and care for with few user-serviceable components. Maintenance is primarily keeping the instrument and rotor clean (see Section 4.2). Other preventive measures are listed below: •... -
Page 29: Cleaning And Decontamination Procedures
SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures The rotor and chambers are designed to reduce the risk of fluid escaping into the rotor interior during cytocentrifugation. However, the chambers do not fully eliminate biohazard contamination risks. Introducing samples into the chambers, insecurely placed caps, improper placement of chambers, and/or exceeding the maximum volume can lead to contamination of the rotor interior. -
Page 30: Chemically Disinfecting The Rotor
SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures Chemically Disinfecting the Rotor To chemically disinfect for Human Immunodeficiency Virus (HIV) or Mycobacterium tuberculosis: Spray with diluted (1/256 x 30 mL/gallon of water) Vesphene II SE* or some other intermediate level disinfecting detergent and soak for at least 20 minutes. -
Page 31: Chemically Disinfecting Single Or Dual Chambers
SECTION 4 CLEANING AND MAINTAINING CYTOPRO 4.2 Cleaning and Decontamination Procedures Chemically Disinfecting Single or Dual Chambers Remove used Cytopads as described in Section 3.1. Submerge chambers and frames in diluted (1/10) household bleach. Make dilution fresh each day. Soak for at least one hour. Scrub interior chamber surfaces with detergent-soaked cotton-tipped swab to remove residual cells. -
Page 32: Shipping Or Disposing Of The Rotor
SECTION 4 CLEANING AND MAINTAINING CYTOPRO 4.3 Shipping or Disposing of the Rotor Shipping the Rotor WARNING! You must disinfect the rotor before returning it to Elitech Group. The operating authority must complete a Hazard Free Certification Form (see below), otherwise the distributor or service center may not accept the instrument;... -
Page 33: Customer Service
Elitech Group’s Service Department will help you resolve any questions about the operation or performance of your Aerospray stainer/cytocentrifuge. Customers in the United States should contact us by telephone. Outside the U.S., our authorized dealers offer full local service and support. ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 USA Telephone: 800 453 2725 (United States &... -
Page 34: Appendix A: Critical Reagent Components
APPENDIX Critical Reagent Components The following information is to identify the critical chemicals of each reagent used in this instrument. SS-133 Decontamination Solution Concentrate contains: <30% Germicidal Detergent >70% Deionized Water SS-133 Decontamination Solution Concentrate is associated with the following Hazard and Safety Precautionary Statements. -
Page 35: Appendix B: Accessories And Supplies
APPENDIX Accessories and Supplies Only replacement parts supplied by Elitech Group should be used with the Cyopro Cytocentrifuge. Use of non- approved parts may affect the performance and safety features of this product. ACCESSORIES REFERENCE NUMBER Cytopro Cytocentrifuge Rotor ....................AC-160 SUPPLIES REFERENCE NUMBER Cytopro Rotor Maintenance Kit ....................
Need help?
Do you have a question about the CYTOPRO AC-160 and is the answer not in the manual?
Questions and answers