ELITechGroup AEROSPRAY TB Applications Manual
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ELITechGroup AEROSPRAY TB Applications Manual

Slide stainer/cytocentrifuge
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Applications
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AEROSPRAY
TB
MODEL 7722
SLIDE STAINER/CYTOCENTRIFUGE
®

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  • Page 1 ® AEROSPRAY MODEL 7722 Applications Manual SLIDE STAINER/CYTOCENTRIFUGE...
  • Page 2 ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 800 453 2725 +1 435 752 6011 WWW.ELITECHGROUP.COM...
  • Page 3 ® AEROSPRAY SLIDE STAINER/CYTOCENTRIFUGE Model 7722 Applications Manual 57-2004-01E...
  • Page 4 ©2015 ELITechGroup Inc. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means whatsoever, without the prior express written permission of ELITechGroup Inc.

  • Page 5: Table Of Contents

    Table of Contents Section 1 Introduction 1.1 Aerospray TB Stainer Overview ............7 Using this Manual ..............7 Functional Description ............8 Key Features ................. 9 Intended Use ................ 9 Table 1: General Specifications..........10 Table 2: Performance Specifications........11 Table 3: Carousel and Rotor Information ......
  • Page 6 Section 3 Controlling and Customizing Stainer Functions (continued) Using Reagent Information Tracking ........38 Modifying Level Detect Functions ........39 Changing User Language ............39 Setting the Date and Time ............ 39 System Log ................40 Accessing Logs ..............40 Exporting Logs ..............
  • Page 7 Section 5 Preventive Maintenance and Safety (continued) 5.5 Decontaminating the Stainer and Carousels ........60 Solutions for Decontaminating the Instrument ....60 Figure 13: Lid Latch and Locking Pin Hole Locations .... 61 Decontaminating the Instrument ......... 61 Decontaminating the Reagent D Bottle........ 62 5.6 Shipping or Disposing of the Stainer or Carousels......
  • Page 8 Appendix D Programming Options (Continued) Primary Wash Setting ........... 97 Decolorizer Setting ............97 Decolorizer Wash Setting ..........98 Counterstain Setting ............. 98 Counterstain Wash Setting ........... 98 Dry Time Options ............98 Table A: 12-Slide Carousel Timing ........ 98 Table B: 30-Slide Carousel Timing .........

  • Page 9: Aerospray Tb Stainer Overview

    INTRODUCTION 1.1 Aerospray TB Stainer Overview Using this Manual This manual provides instructions to install, operate, and maintain the Aerospray TB Stainer/Cytocentrifuge. The manual is an important part of the product. Read it carefully and completely before setup and first use of the instrument.

  • Page 10: Functional Description

    The TB stainer is capable of either fluorescence or carbol fuchsin staining, depending on the reagents loaded on the instrument. For best results when using the Aerospray TB stainer, body fluid specimens should be digested and spread as thin as possible on the...

  • Page 11: Key Features

    The use of Aerospray TB reagents is required for successful use of this instrument. Adding the optional Cytopro rotor allows...
  • Page 12 SECTION 1 INTRODUCTION 1.1 Aerospray TB Stainer Overview Table 1: General Specifications Category Characteristics Slide Carousel Capacity 1 to 12 or 1 to 30, depending on carousel Carousel Rotation Speed Reagent application 10 to 20 rpm ( 5%) max. Slide drying 200 to 950 rpm ( 5%) max.
  • Page 13 SECTION 1 INTRODUCTION 1.1 Aerospray TB Stainer Overview Table 1: General Specifications (continued) Category Characteristics Pollution Degree Heat Dissipation Maximum 512 Btu/hour Average During Staining 102 Btu/hour Average While Idle 41 Btu/hour Maximum Sound Emission 72 dB Table 2: Performance Specifications...
  • Page 14 SECTION 1 INTRODUCTION 1.1 Aerospray TB Stainer Overview Table 3: Carousel and Rotor Information Only the following slide staining carousels or cytocentrifuge rotor can be used in this instrument. Each should be used following the instructions in this manual or the Cytopro Applications Manual (RP-517).
  • Page 15 SECTION 1 INTRODUCTION 1.1 Aerospray TB Stainer Overview Table 4: Explanation of Symbols Alternating Current (AC) Authorized Representative in the European Community Batch Code Biological Hazards (Biological Risks) Catalog Number (Model Number) Caution, Consult accompanying documents (Attention, see Instructions For Use) CE Mark, product meets the essential requirements designated in Annex I of the In Vitro Device Directive (IVDD) 98/79/EC.
  • Page 16 SECTION 1 INTRODUCTION 1.1 Aerospray TB Stainer Overview Table 4: Explanation of Symbols (continued) “On” (Power) “Off” (Power) Environment Friendly Use Period Serial Number Temperature Limitation – indicates high and low limits (Normal room temperatures are specified for all Elitech Group Reagents.)

  • Page 17: Instrument Description

    SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 1: Front and Right Side Panels 1 – Optional 30-slide Carousel 2 – Front Panel with Touch Screen Display 3 – Bowl 4 – 12-slide Carousel 5 – Lid with Safety Lock 6 – Right Side Panel with Label Indicating Reagent Positions: •...
  • Page 18 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 2: Front Panel and Touchscreen 1 – Standby/Ready Button 2 – Touchscreen The front panel features an interactive touchscreen display. Refer to Touchscreen and User Interface (Section 1.3) for more information. Figure 3: Rear Panel 2 2 3 11 10 9 1 –...
  • Page 19 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 4: Stainer Bowl Components 1 – Nozzle D (Deionized or Distilled Water, front) 2 – Nozzle A (Decolorizer) 3 – Nozzle B (Counterstain) 4 – Nozzle C (Primary Stain) 5 – Nozzle E (Approved Alcohol) 6 –...
  • Page 20 SECTION 1 INTRODUCTION 1.2 Instrument Description Table 5: Preventive Maintenance Kit Component Name Description Manual Priming Tool Primes air-locked pumps Silicon Grease Lubricates the nozzle threads for easy assembly Nozzle Wire Cleans nozzle housing orifices Nozzle Cleaning Strainer Holds nozzle parts to prevent loss during cleaning Nozzle Tool Unscrews nozzles from the stainer...

  • Page 21: Barcode Reader

    For soaking nozzles in the Nozzle (large tube) Cleaning Solution Barcode Reader An optional barcode reader is available for use with the Aerospray TB Stainer/Cytocentrifuge (Model 7722). Figure 6: Barcode Reader Other Needed Items The following parts are not available worldwide from Elitech Group, but they can be obtained locally: •...

  • Page 22: Touchscreen And User Interface

    SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Users control all instrument functions from the interactive touchscreen display. Table 6: Front Panel/Main Screen Function Keys Button Name Description Standby/Ready With instrument power ON: Blue = Ready Amber = Standby Pressing Standby runs a Clean cycle and places instrument in standby mode The Standby/Ready button also accesses the touchscreen calibration function (Section 7.4).
  • Page 23 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 6: Front Panel/Main Screen Function Keys (continued) Button Name Description Back Returns to the previous menu Stop Aborts any operation Indicates completion of current task System Setup Allows users to modify the software settings. See System Setup menu, (Section 3.1) Table 7: System Setup Keys Button...
  • Page 24 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 7: System Setup Keys (continued) Button Name Description Set Date/Time Allows users to set the date and time Restore Defaults Restores programming to default settings Login Enters Login sequence for authorized users Logout Logs authorized users out.
  • Page 25 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 8: Maintenance Function Keys Button Name Description Prime A, B, C, D, E Primes the selected line Prime ABCDE Primes all lines simultaneously Pattern Test Performs Slide Pattern or Hub Pattern Test to ensure nozzles are clear of debris and spraying properly Volume Test...

  • Page 26: Setup And Preparation For Operation

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Unpacking and Installing the Stainer Follow this sequence if you are using this instrument for the first time. Details about these operations are given in the next three sections. • Install the drain tube •...

  • Page 27: Connecting The Drain Tube And Waste Container

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Connecting the Drain Tube and Waste Container Insert the waste tube connector into the rear panel receptacle until you hear a click. Adjust the tube length to less than 1.8 m (72 in.). CAUTION: Keep the drain tube straight and as short as possible.

  • Page 28: Installing Standard 500 Ml Reagent Bottles

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Installing Standard 500 mL Reagent Bottles WARNING! Reagents used in the instrument contain moderately hazardous chemicals that require care in handling. Always handle reagents using appropriate safety measures, including gloves and eye protection. NOTE: Reagents should be stored according to the conditions specified on their label.
  • Page 29 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup NOTE: See Appendix A and Appendix D for complete identification of all reagents used in this stainer. CAUTION: To avoid severe damage, never use reagents containing organic solvents in this instrument, unless supplied by Elitech Group, or specified in official Elitech Group formulation instructions.

  • Page 30: Installing The 5 Liter Reagent Bottle

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Installing the 5 Liter Reagent Bottle NOTE: A 5 Liter (L) bottle for reagent D is available. If using the 5 L bottle, install it near the side or back of the instrument not in the instrument tray. NOTE: If using the 5 L bottle, you must replace the standard dip tube with the longer dip tube that comes with the 5 L bottle.

  • Page 31: Preparing The Stainer For Operation

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures NOTE: The instrument is shipped with alcohol in the reagent lines. For proper performance, this alcohol must be replaced with the correct reagent for each reagent line prior to use.
  • Page 32 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures (continued) Press 60-Sec Prime to prime each reagent line with at least 200 mL of reagent to remove all of the alcohol from the reagent lines and pumps. Choose one of the following: •...

  • Page 33: The Clean Cycle

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation The Clean Cycle NOTE: The Clean cycle uses 20 mL of alcohol divided equally among nozzles C and E to clean the carousel and stainer bowl after staining. Water is sprayed from the D and B nozzles and Reagent A is sprayed from the A nozzle.

  • Page 34: Reagent Level Monitoring

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Reagent Level Monitoring Reagent Level Detect monitors reagent levels and alerts you when the reagent is running low, or when the waste container is full (when using the waste container with level detect).

  • Page 35: Zeroing The Reagent Level Sensors

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Zeroing the Reagent Level Sensors The Level Detect function must be zeroed at initial setup, when the stainer is moved, or if the level detect is not reporting correctly. If zeroing does not correct the problem, recalibrate the Level Detect function (Section 7.3).
  • Page 36 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Remove all reagent bottles and press Start. The display shows: NOTE: Vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. After zeroing, press OK.

  • Page 37: Controlling And Customizing Stainer Functions

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Many software settings can be controlled from the System Setup menu, including: • Creating, editing, and deleting stain programs • Creating, editing, and deleting cytocentrifuge programs • Tracking reagent information •...

  • Page 38: Editing A Stain Program Or Adjusting Stain Settings

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Editing a Stain Program or Adjusting Stain Settings From System Setup, press Stain Programs. Select the program to be modified. Select the Primary/Counterstain or Counterstain/Dry tab. Modify the desired staining parameters using the sliding tabs. Press Save.

  • Page 39: Administrator And User Accounts

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Administrator and User Accounts You can create one Administrator account and multiple (up to 50) user accounts. The administrator controls access to the system by adding and editing user accounts. Users cannot edit System Settings unless permitted by the administrator.

  • Page 40: User Login/Logout

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Managing User Access (continued) • Enable Run Login requires the current user to enter a password to run a Stain or Cytocentrifuge cycle. Global Login must be enabled to use this option.

  • Page 41: Modifying Level Detect Functions

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Using Reagent Information Tracking (continued) Select Change next to the appropriate reagent. Scan the reagent bottle barcodes (Section 3.2) or manually enter the reagent information in the correct fields. Select Save for each reagent.

  • Page 42: System Log

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu System Log The instrument records all login, logout, stain or cytocentrifuge cycles, setting changes, maintenance functions and specimen identification (if enabled). Accessing Logs From System Setup, press System Log. Use navigation arrows to scroll through the log.

  • Page 43: Qc/Maintenance Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu QC/Maintenance Tracking Under system default settings, The following QC/Maintenance Tracking options are disabled: • Enable Stain Slide Tracking • Enable Cyto Slide Tracking • Manual Entry • Enable Preventive Maintenance Tracking •...

  • Page 44: Enable Cyto Slide Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Enable Cyto Slide Tracking Allows slide tracking in cytocentrifuge mode. See the Cytopro Applications Manual for complete information. Enable Manual Entry If selected, allows manual entry of slide information using the keypad (limited to 24 characters).

  • Page 45: Restoring Software Defaults

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Restoring Software Defaults From System Setup, select Restore Defaults. CAUTION: Restoring the system defaults will remove all personal settings. • Restoring System Settings will delete all user names and passwords as well as all stain and cytocentrifuge programs.

  • Page 46: Recording Specimen And Reagent Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Slides with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Stain Slide Tracking. NOTE: Selecting Enable Stain Slide Tracking changes the Start button on the Main menu to “Load Slides.”...

  • Page 47: Scanning Reagent Bottles With The Barcode Reader

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Reagent Bottles with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Reagent Tracking for each desired reagent (A, B, C, D, E). Press Back to return to the System Setup menu. Press Reagents to access the Reagent Information screen.

  • Page 48: Manually Entering Specimen Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Reagent Bottles with the Barcode Reader (continued) Press Save. Repeat steps 3-8 for each reagent bottle that is enabled in QC Maintenance Tracking. NOTE: You can access Reagent Information by pressing the bottle icons on the right side of the Main menu.

  • Page 49: The Help Menu

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.3 The Help Menu The Help menu is a comprehensive onscreen help function that provides detailed information on the following subjects: Basic Operation • Loading the Carousel • Correct Reagents and Locations • Nozzle Volumes •...

  • Page 50: Operating The Stainer

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Suggested Staining Protocol NOTE: Samples and slides should be prepared and fixed according to recommendations in Appendix E or equivalent. • Hub Pattern Test (once per day). • If slide tracking is enabled, scan or enter slide information. •...

  • Page 51: Loading The Carousel

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Loading the Carousel CAUTION: Never load chipped or cracked slides into the carousel. Slides in poor condition may break during the staining cycle. If a slide breaks in the bowl, refer to Cleaning Broken Slides, Section 5.4.
  • Page 52 SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Loading the Carousel (continued) • If using a 12-slide carousel, load slides with the labels toward the outer rim of the carousel. • If using a 30-slide carousel, load slides with the labels toward the center of the carousel.

  • Page 53: Using Blocking Slides

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Using Blocking Slides If the carousel is not full, blank slides should be used as blocking slides. Blocking slides prevent overspray of reagents onto the specimen slides. Overspray can cause slides to become over-decolorized.

  • Page 54: Unloading The Carousel

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Performing a Stain Cycle (continued) If you have created a stain program, and it appears on the display, proceed to Step 4. If the desired program does not appear on the display, select Programs.

  • Page 55: Monitoring Reagent And Waste Levels

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Monitoring Reagent and Waste Levels If enabled, the stainer displays the approximate reagent and waste container levels and other information. CAUTION: You must monitor the reagent and waste container levels on the display (if enabled) and by direct inspection of the bottles.

  • Page 56: Replacing A Reagent Bottle

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions NOTE: You can access the Reagent Information menu by pressing the bottle icons on the right side of the Main menu. Press Change to scan or manually enter reagent information. NOTE: Do not put residual reagent from a used bottle into a new bottle. This can lead to an accumulation of residue on the slides and may be a source of contamination.

  • Page 57: Preventive Maintenance And Safety

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance The system provides a Preventive Maintenance Log for tracking the most recent maintenance activities. See Enable Preventive Maintenance Tracking in Section 3 and Using the Preventive Maintenance Log in this section. Daily Maintenance/Quality Control (QC) Check reagent levels and expiration dates.

  • Page 58: Monthly Maintenance

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance Monthly Maintenance Disassemble and manually clean all nozzles. Refer to Nozzle Disassembly and Cleaning (Section 6.1). Disinfect the Reagent D bottle (Section 5.5). If using potassium permanganate, flush the B-line (Section 6.5). Ensure the maintenance procedures on the Preventive Maintenance (PM) Log have been performed, and entered into the PM chart or log.

  • Page 59: Storing The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.2 Storing the Instrument If the instrument is inactive for more than one week, you may want to perform the long-term storage procedure. This will prevent nozzles and internal valves from clogging when the machine is reactivated.

  • Page 60: Replacing Fuses

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.3 Replacing Fuses WARNING! To prevent the risk of fire, the main fuses should only be replaced with fuses of the same type and rating. Recurring fuse failure indicates serious internal problems, if this occurs, contact Elitech Group.

  • Page 61: Cleaning The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.4 Cleaning the Stainer and Carousels WARNING! All cleaning procedures should be performed in a well-ventilated room by authorized trained personnel wearing appropriate protection equipment. Clean the outside of the instrument with 70 to 100% alcohol. Clean the carousel and lid with 70 to 100% alcohol.

  • Page 62: Decontaminating The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels All parts of the instrument that come into contact with biological specimens, patient specimens, positive control specimens, or hazardous material must be treated as potentially infectious. Before the instrument is returned for service, all outer surfaces must be decontaminated. The operating authority must complete a disinfection declaration, otherwise the instrument may be rejected by the distributor or service center or quarantined by customs authorities.

  • Page 63: Decontaminating The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Figure 13: Lid Latch and Locking Pin Hole Locations 1 – Lid Latch Hole 2 – Locking Pin Hole Decontaminating the Instrument Prepare a suitable container for all disposables. Cover the lid latch and locking-pin holes with waterproof tape to protect the interior (Figure 13).

  • Page 64: Decontaminating The Reagent D Bottle

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Decontaminating the Reagent D Bottle Fill the reagent D (deionized or distilled water) bottle with a fresh (< 24 hours old) 10% dilution of household bleach solution (5.25% sodium hypochlorite).

  • Page 65: Shipping Or Disposing Of The Stainer Or Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.6 Shipping or Disposing of the Stainer or Carousels Shipping the Instrument WARNING! You must disinfect the carousel before returning it to Elitech Group. The operating authority must complete a disinfection declaration, otherwise the distributor or service center may not accept the instrument;...

  • Page 66: Nozzle Maintenance And Performance

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.1 Nozzle Disassembly and Cleaning Nozzle maintenance requires the Nozzle Maintenance Kit and Cleaning Solution (diluted SS-029C or SS-266). WARNING! Always wear protective clothing and eye protection when using Cleaning Solution (diluted SS- 029C or SS-266). Dispose of used solution properly. Nozzle Disassembly Remove the nozzle using the nozzle tool from the Nozzle Maintenance Kit.

  • Page 67: Nozzle Reassembly

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.2 Nozzle Reassembly Hold your thumb or a strainer over the end of the tube to keep the nozzle parts in the tube. Pour out the liquid used to clean the nozzle parts. Swirl Cone Inspect the nozzle parts.

  • Page 68: Manual Priming

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.3 Manual Priming Remove the carousel from the bowl. Remove the nozzle connected to the reagent line to be manually primed. Insert the priming tool nozzle adapter (included in the Nozzle Maintenance Kit) into the nozzle holder. Turn the nozzle adapter clockwise to install the adapter into the holder.

  • Page 69: Performing The Volume Test

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.4 Performing the Volume Test The Volume Test requires the Nozzle Maintenance Kit. NOTE: The Volume Test must be performed weekly. From the Maintenance menu, select Volume Test. Hold a Volume Test tube (small tube) to cover the selected nozzle. Press the corresponding reagent button to collect the reagent.
  • Page 70 NOZZLE MAINTENANCE AND PERFORMANCE 6.4 Performing the Volume Test b. If necessary, remove the nozzle and perform the Nozzle Cleaning procedure (Section 6.1). If the problem persists, replace the nozzle. NOTE: If the problem persists after you have replaced the nozzle, contact Elitech Group.

  • Page 71: Using The Line Flush

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.5 Using the Line Flush This procedure may be performed when troubleshooting a staining issue. It is also required when switching from carbol fuchsin to fluorescence staining reagents. This procedure requires the Nozzle Maintenance kit. You can flush the B or C line separately or both lines at the same time. The B-line must be flushed at least once per month when using potassium permanganate.
  • Page 72 SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.5 Using the Line Flush NOTE: You can still use the Cytocentrifuge mode during this time by selecting Cyto. 11 Load 500 mL of deionized or distilled water into the line(s). 12 Press Start. The instrument will pump approximately 400 mL of water through the line(s).

  • Page 73: Performing The Slide Pattern Test

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.6 Performing the Slide Pattern Test This test can differentiate poor staining results from sample preparation problems, or nozzle obstructions. Perform the Slide Pattern test when a Hub Pattern test produces a normal result, but staining is still inadequate.

  • Page 74: Solving Problems

    SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting This section helps you identify and solve routine problems with the stainer. More difficult problems may require technical service. Contact your Elitech Group representative for assistance. WARNING! Due to the electrical shock hazard, do not open this instrument or attempt internal repairs. Refer servicing to qualified service personnel.
  • Page 75 SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 12: General Troubleshooting and Diagnosis (continued) Problem Solution Stain is leaking onto the counter. Check all external reagent lines for visible signs of cracks or loose fittings. Make sure the drain outlet is not blocked. Make sure the drain tube is securely attached to the drain port and that the tubing is not cracked or deformed.
  • Page 76 SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 12: General Troubleshooting and Diagnosis (continued) Problem Solution The stainer fails to spray reagent during a staining cycle To allow programmed staining of partial loads, the and/or continues to run after the cycle should be stainer monitors the position of the carousel as it rotates complete.
  • Page 77 SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 12: General Troubleshooting and Diagnosis (continued) Problem Solution Abnormal staining on entire surface of all slides When staining a full carousel (7 or more slides for the 12- (continued). slide carousel or 17 or more for the 30-slide carousel), make certain you have not programmed the stainer for fewer slides.
  • Page 78 SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 12: General Troubleshooting and Diagnosis (continued) Problem Solution Specimens are washing off slides. Try to make your smears as thin as possible for a given specimen, to minimize fixation problems. Always use clean, premium quality slides. Review sample fixation procedures in Appendix E.

  • Page 79: Abnormal Staining Results

    SECTION 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Smear Separation or Cell Loss Possible causes for losing smears from the slide surface during a staining cycle: • Improper fixation • Reagent application force-poor nozzle spray • Wet smears • Thick smears •...
  • Page 80 SECTION 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Dilution: 150 mL household bleach + 850 mL deionized water = 1000 mL NOTE: Do not remove nozzles. Pump 150 mL of prepared solution through each affected reagent line (B, C and D). Wait 20 minutes. NOTE: It is unlikely for the A line to have precipitate build up or bacterial contamination due to the chemical makeup of Reagent A.

  • Page 81: Instrument Malfunction

    SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Air or Reagent Leaks Repriming the instrument is usually unnecessary unless a reagent bottle runs completely dry. An air leak is usually to blame if a smooth and continuous liquid spray fails to come from the nozzles.

  • Page 82: Reagent Level Detect System Errors

    SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Reagent Level Detect System Errors Reagent A-E Not Calibrated During the second part of calibration, if no bottles are detected, the display shows an error message. Calibrate again, making sure that the correct reagent bottles are placed in the enabled tray positions.

  • Page 83: Calibrating The Reagent Level Detect System

    SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Calibrating the Reagent Level Detect System If the Reagent Level Detect System is reporting incorrectly and zeroing (Section 2.2) does not correct the problem, calibrate the system as follows: Press System Information from the Main menu. Press System Setup.
  • Page 84 SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Calibrating the Reagent Level Detect System (continued) NOTE: Any vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. NOTE: Calibration requires full, unopened (caps and seals in place) 500 mL bottles of reagent, placed in the correct tray positions (due to different densities of each reagent type).

  • Page 85: Calibrating The Touchscreen

    SECTION 7 SOLVING PROBLEMS 7.4 Calibrating the Touchscreen Select and hold Standby/Ready for 5 seconds. A calibration screen with a target appears. Select the center of the target with a finger, stylus, or similar tool. Another target will appear in a different location. Continue to press the center of the targets until you have pressed all the targets (five total).

  • Page 86: Customer Service

    Elitech Group’s Service Department will help you resolve any questions about the operation or performance of your Aerospray Stainer/Cytocentrifuge. Customers in the United States should contact us by telephone. Outside the U.S., our authorized dealers offer full local service and support. ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 USA Telephone: 800 453 2725 (United States &...

  • Page 87: Cytopro® Cytocentrifuge

    SECTION 8 CYTOPRO® CYTOCENTRIFUGE 8.1 Cytopro Cytocentrifuge Information Functional Description The Cytopro Cytocentrifuge rotor allows rapid sedimentation of specimen cells onto microscope slides for staining or other purposes. Up to eight disposable/reusable sample chamber assemblies with absorbent pads and glass microscope slides can be loaded into the Cytocentrifuge rotor.

  • Page 88: Appendix A: Critical Reagent Components

    Critical Reagent Components The following information identifies the critical chemicals of each reagent used in this instrument. Reagent(s) Critical Components SS-061A or SS-061A-EU Aerospray TB Reagent A Carbol >90% Methyl Alcohol Fuchsin Decolorizer; <2% Nitric Acid SS-161A Aerospray TB Reagent A Carbol Fuchsin...
  • Page 89 APPENDIX Critical Reagent Components Reagent(s) Critical Components SS-061M or SS-061M-EU Aerospray TB Mycohold Cell <1% Sodium Azide Adhesive; SS-161M Aerospray TB Mycohold Cell Adhesive Concentrate when diluted properly contains: SS-161A Aerospray TB Reagent Carbol Fuchsin <30% Nitric Acid Decolorizer Concentrate contains: SS-161AF Aerospray TB Reagent Fluorescence <10% Nitric Acid...

  • Page 90: Appendix B: Risk And Safety Phrases

    APPENDIX Risk and Safety Phrases Reagents SS-061A and SS-061A-EU – Aerospray TB Reagent A Carbol Fuchsin Decolorizer and SS- 161A - Aerospray TB Reagent A Carbol Fuchsin Decolorizer concentrate when properly diluted are associated with the following Risk and Safety Phrases. The associated EU Symbols of Danger are: F and T.
  • Page 91 APPENDIX Risk and Safety Phrases Reagent SS-061BRT-EU – Aerospray TB Reagent B Thiazin Red is associated with the following Risk and Safety Phrases. The associated EU Symbol of Danger is: none None None Reagent SS-061BCS-EU – Aerospray TB Reagent B Carbol Solution is associated with the following Risk and Safety Phrases.
  • Page 92 Safety Phrases. The associated EU Symbol of Danger is: none. None None SS-161M – Aerospray TB Mycohold Cell Adhesive Concentrate is associated with the following Risk and Safety Phrases. The associated EU Symbol of Danger is Xn. R 22...
  • Page 93 APPENDIX Risk and Safety Phrases SS-161BBG – Aerospray TB Reagent Brilliant Green Concentrate is associated with the following Risk and Safety Phrases. The associated EU Symbol of Danger is none. R 10: Flammable None SS-161BMB – Aerospray TB Reagent Methylene Blue Concentrate is associated with the following Risk and Safety Phrases.
  • Page 94 APPENDIX Risk and Safety Phrases SS-MeOH Aerospray Methanol is associated with the following Risk and Safety Phrases. European Symbols of Danger are: F and T. R 11: Highly Flammable R 23/24/25: Toxic by inhalation, in contact with skin and if swallowed Toxic: danger of very serious irreversible effects through inhalation, in contact with 39/23/24/2 skin and if swallowed...

  • Page 95: Appendix C: Accessories And Supplies

    APPENDIX Accessories and Supplies Only replacement parts supplied by Elitech Group should be used in this instrument. Use of non-approved parts may affect the performance and safety features of this product. ACCESSORIES REFERENCE NUMBER Slide Carousel (12-Slide Capacity) ............AC-188 Slide Carousel (30-Slide Capacity) ............AC-189 Nozzle Tool ....................AC-034 Nozzle Hex Wrench ..................AC-035 5 L Space-Saver Container w/cap (For Concentrate Reagents) ....AC-038...
  • Page 96 Preventive Maintenance Chart, pad of 24 sheets ........SS-267 PM Cleaning Solution, 500 mL ..............SS-266 or SS-266-EU Aerospray TB (Model 7722) Applications Manual ........RP-464 Cytopro Cytocentrifuge Rotor (Model AC-160) Applications Manual ..RP-517 Contact Elitech Group for a complete list of replacement parts.

  • Page 97: Appendix D Programming Options

    Programming Options General Programming Information • The Aerospray TB stainer uses two acid-fast staining options: carbol fuchsin or fluorescence. Each option can be modified by the choice of reagents and program settings. • The stainer comes with two default programs (one fluorescence and one carbol fuchsin) that have been designed to meet the needs of most users without adjustment.
  • Page 98 APPENDIX Programming Options CAUTION: The stainer is not capable of detecting which reagents are loaded on the instrument. The user must take care to load the correct reagents in the correct positions, based upon the options given here and according to user preference. Reagent Options for Carbol Fuchsin Staining (by position) (A) Reagent: A Carbol Fuchsin Decolorizer: •...

  • Page 99: Stain Program Options

    APPENDIX Programming Options Potassium Permanganate Stains: • Ready to use: SS-061BP or SS-061BP-EU • Concentrate: SS-161BP Thiazin Red Stain: • Ready to use: SS-061BRT-EU and SS-061BCS-EU (Reagents are mixed 50: 50 before use. Mixture is stable for 2 days after mixing.) NOTE: Thiazin Red is available in Europe as a replacement for Potassium Permanganate.

  • Page 100: Decolorizer Wash Setting

    APPENDIX Programming Options Decolorizer Wash Setting Increase setting to increase washing, a higher setting cleans the slides better and helps even decolorization across the slide. Counterstain Setting Increase setting to increase intensity of the background staining counterstain. Counterstain Wash Setting Increase setting to increase washing, a higher setting cleans the slides better after counterstain step.
  • Page 101 APPENDIX Programming Options Decolorizer Setting Decolorizer, total number of applications per slide Decolorizer, total wait time (sec.) Approximate reagent usage for a full carousel (mL)* 10.5 12 13.5 15 16.5 18 Decolorizer Wash Setting Decolorizer Wash, total number of applications per slide Approximate water (D-line) usage for a full carousel (mL)*...
  • Page 102 APPENDIX Programming Options Primary Wash Setting Primary Wash, total number of applications per slide Approximate water (D-line) usage for a full carousel (mL)* Decolorizer Setting Decolorizer, total number of applications per slide Decolorizer, total wait time (sec.) Approximate reagent usage for a full carousel (mL)* Decolorizer Wash Setting Decolorizer Wash, total number of applications per slide...

  • Page 103: Appendix E Sample/Slide Preparation

    Hydroxide Method has been reported to be an appropriate liquefying and decontamination method for sputum samples stained with the TB stainer. Use this technique or an equivalent on specimens intended for staining in the Aerospray TB stainer. The N-acetyl-L-cysteine-sodium Hydroxide Method is summarized as follows Principle: Sodium hydroxide (NaOH), a decontaminating agent, also acts as an emulsifier.
  • Page 104 APPENDIX Sample/Slide Preparation Work within a biological safety cabinet and wear protective clothing, gloves, and mask. Transfer a maximum of 10 mL of sputum, urine, or other fluid to be processed to a sterile, disposable plastic 50-mL conical centrifuge tube with a leak-proof and aerosol-free plastic screw cap. Tubes with easily visible volume indicator marks are best.

  • Page 105: Sample Fixation

    Elitech Group recommends using Mycohold™. Mycohold was developed by Elitech Group to stabilize specimen stained in the Aerospray TB stainer. This proprietary protein solution (REF: SS-061M) is also available as a 10X concentrate (REF: SS-161M). Suggested directions for use are given below.

  • Page 106: Staining Slides

    APPENDIX Sample/Slide Preparation Using Mycohold Cell Adhesive (continued) Procedure B Add 1 part SS-161M and 9 parts of liquid specimen and mix well. Apply the specimen containing the Mycohold to a microscope slide and allow to dry. Heat dried specimen at 65 to 70 °C for at least 15 minutes. Process the slides as usual.

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