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ELITechGroup AEROSPRAY GRAM 7322 Applications Manual

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Applications
Manual
AEROSPRAY
GRAM
MODEL 7322
SLIDE STAINER/ CYTOCENTRIFUGE
®

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Summary of Contents for ELITechGroup AEROSPRAY GRAM 7322

  • Page 1 ® AEROSPRAY GRAM MODEL 7322 Applications Manual SLIDE STAINER/ CYTOCENTRIFUGE...
  • Page 2 ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 800 453 2725 +1 435 752 6011 WWW.ELITECHGROUP.COM...
  • Page 3 ® AEROSPRAY GRAM SLIDE STAINER/CYTOCENTRIFUGE Model 7322 Applications Manual 57-2002-01D...
  • Page 4 ©2015 ELITechGroup Inc. All rights reserved. Printed in the United States of America. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means whatsoever, without the prior express written permission of ELITechGroup Inc.

  • Page 5: Table Of Contents

    Table of Contents Section 1 Introduction 1.1 Aerospray Gram Stainer Overview ..........6 Using this Manual ..............6 Functional Description ............7 Key Features ................. 8 Intended Use ................ 8 Table 1: General Specifications..........9 Table 2: Performance Specifications........10 Table 3: Run Time Sequence ..........
  • Page 6 Section 3 Controlling and Customizing Stainer Functions (continued) Creating User Accounts ..........34 Managing User Access ........... 35 User Login/Logout ............35 Using Reagent Information Tracking ........36 Modifying Level Detect Functions ........37 Changing User Language ............37 Setting the Date and Time ............ 37 System Log ................
  • Page 7 Section 5 Preventive Maintenance and Safety (continued) 5.4 Cleaning the Stainer and Carousels ..........57 Cleaning Liquid Spills ............57 Cleaning Broken Slides ............57 5.5 Decontaminating the Stainer and Carousels ........58 Solutions for Decontaminating the Instrument ....58 Figure 13: Lid Latch and Locking Pin Hole Locations ..

  • Page 8: Aerospray Gram Stainer Overview

    SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Using this Manual This manual provides instructions to install, operate, and maintain the Aerospray Gram Stainer/Cytocentrifuge. The manual is an important part of the product. Read it carefully and completely before setup and first use of the instrument. If additional accident prevention and environmental protection requirements exist in the country of operation, this manual must be supplemented by appropriate instructions to ensure compliance.

  • Page 9: Functional Description

    To avoid serious instrument damage, never use reagents other than those supplied by Elitech Group. Except for deionized or distilled water and approved alcohol, using reagents not supplied by ElitechGroup may void the warranty. CAUTION: Only spare parts supplied or specified by Elitech Group should be used. Using non- approved parts may affect the performance and safety features of the instrument.

  • Page 10: Key Features

    SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Key Features The key features of the Aerospray Gram stainer are: • Minimized reagent consumption • Rapid staining • Barcode scanner for tracking specimens and reagents • Reagent and specimen traceability • User traceability •...
  • Page 11 SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Table 1: General Specifications Category Characteristics Slide Carousel Capacity 1 to 12 or 1 to 30, depending on carousel Carousel Rotation Speed Reagent application 20 rpm ( 5%) Slide Drying 950 rpm ( 5%) Cytocentrifuge Rotor Speed 100 to 2000 rpm (...
  • Page 12 Each reagent has a separate spray nozzle to dispense the correct amount of reagent. Reagents A - Decolorizer NOTE: Use only ElitechGroup reagents, with diluents as B - Iodine specified for ElitechGroup concentrated reagents. REF numbers for this stainer start with one of the C - Crystal violet following: SS-041 or SS-141.
  • Page 13 SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Table 3: Run Time Sequence NOTE: Table 3 represents a typical timing sequence for this instrument, presented for general reference only. Actual cycle times may vary. DECOLORIZER-3 EVENT SEQUENCE (SECONDS) (SECONDS) 12–Slide 30-Slide Start-Up Fixation (Pump E) (optional) - Normal...
  • Page 14 SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Table 5: Carousel and Rotor Information Only the following slide staining carousels or cytocentrifuge rotor can be used in this instrument. Each should be used following the instructions in this manual or the Cytopro Applications Manual (RP-452).
  • Page 15 SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Table 6: Explanation of Symbols Alternating Current (AC) Authorized Representative in the European Community Batch Code Biological Hazards (Biological Risks) Catalog Number (Model Number) Caution, Consult Accompanying Documents (Attention, see instructions for use) CE Mark, product meets the essential requirements designated in Annex I of the In Vitro Device Directive (IVDD) 98/79/EC.
  • Page 16 SECTION 1 INTRODUCTION 1.1 Aerospray Gram Stainer Overview Table 6: Explanation of Symbols (continued) “On” (Power) “Off” (Power) Environment Friendly Use Period Serial Number Temperature Limitation – indicates high and low limits (Normal room temperatures are specified for all Elitech Group Reagents.) Use By Volume Control General Warning, Caution, Risk of Danger...

  • Page 17: Instrument Description

    SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 1: Front and Right Side Panels 1 – 30-slide Carousel 2 – 12-slide Carousel 3 – Lid with Safety Lock 4 – Bowl 5 – Front Panel with Touch Screen Display 6 – Right Side Panel with Label Indicating Reagent Positions: •...
  • Page 18 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 2: Front Panel and Touchscreen 1 – Standby/Ready Button 2 – Touch Screen The front panel features an interactive touchscreen display. Refer to Touchscreen and User Interface (Section 1.3, Table 8) for more information. Figure 3: Rear Panel 2 2 3 11 10 9...
  • Page 19 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 4: Stainer Bowl Components 1 – Nozzle D (Deionized or Distilled Water, front) 2 – Nozzle A (Decolorizer) 3 – Nozzle B (Iodine) 4 – Nozzle C (Crystal Violet) 5 – Nozzle E (Approved Alcohol) 6 –...
  • Page 20 SECTION 1 INTRODUCTION 1.2 Instrument Description Table 7: Preventive Maintenance Kit Component Name Description Manual Priming Tool Primes air-locked pumps Silicon Grease Lubricates the nozzle threads for easy assembly Nozzle Wire Cleans nozzle housing orifices Nozzle Cleaning Strainer Strains the nozzle parts to prevent them from going down a drain Nozzle Tool Unscrews nozzles from the stainer...

  • Page 21: Barcode Reader

    SECTION 1 INTRODUCTION 1.2 Instrument Description Table 7: Preventive Maintenance Kit (continued) Component Name Description Volume Test Collection Collects reagents while performing Tubes (small tube) the Volume Test Nozzle Maintenance Tube Holds Nozzle Cleaning Tubes (large Stand tube) and Volume Test Tubes (small tube) Nozzle Cleaning Tubes For soaking nozzles in the Nozzle...

  • Page 22: Touchscreen And User Interface

    SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Users control all instrument functions from the interactive touchscreen display. Table 8: Front Panel/Main Screen Function Keys Button Name Description Standby/Ready With instrument power ON: Blue = Ready Amber = Standby Pressing Standby runs a Clean cycle and places instrument into standby mode The Standby/Ready button also accesses the touchscreen calibration function.
  • Page 23 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 8: Front Panel/Main Screen Function Keys (continued) Button Name Description Back Returns to the previous menu Stop Aborts any operation Indicates completion of current task System Setup Allows users to modify the software settings. See System Setup menu, (Section 3.1) Table 9: System Setup Keys Button...
  • Page 24 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 9: System Setup Keys (continued) Button Name Description Set Date/Time Allows users to set the date and time Restore Defaults Restore programming to default settings Unselected Shows an unselected option Selected Shows a selected or enabled option Table 10: Maintenance Function Keys Button...

  • Page 25: Setup And Preparation For Operation

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Unpacking and Installing the Stainer Follow this sequence if you are using this instrument for the first time. Details about these operations are given in the next three sections. • Install the drain tube •...

  • Page 26: Connecting The Drain Tube And Waste Container

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Connecting the Drain Tube and Waste Container Insert the waste tube connector into the rear panel receptacle until you hear a click. Adjust the tube length to less than 1.8 m (72 in.). NOTE: Ensure the waste tube has no loops or kinks, and is as straight and as short as possible.

  • Page 27: Installing Standard 500 Ml Reagent Bottles

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Installing Standard 500 mL Reagent Bottles WARNING! Reagents used in the instrument contain moderately hazardous chemicals that require care in handling. Always handle reagents using appropriate safety measures, including gloves and eye protection. NOTE: Reagents should be stored according to the conditions specified on their label.

  • Page 28: Installing The 5 Liter Reagent Bottle

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Installing the 5 Liter Reagent Bottle NOTE: A 5 Liter (L) bottle for reagent D is available. If using the 5 L bottle, install it near the side or back of the instrument not in the instrument tray. •...

  • Page 29: Preparing The Stainer For Operation

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures NOTE: The instrument is shipped with alcohol in the reagent lines. For proper performance, this alcohol must be replaced with the correct reagent for each reagent line prior to use.
  • Page 30 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures (continued) Repeat the previous steps for each nozzle, (B, C, D, and E). When the D prime button is pressed, most of the reagent will come out of the D position.

  • Page 31: The Clean Cycle

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation The Clean Cycle NOTE: The Clean cycle uses 40 mL of alcohol divided equally among nozzles A, B, C and E to clean the carousel and stainer bowl after staining. Pressing Standby/Ready performs the same function.

  • Page 32: Reagent Level Monitoring

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Reagent Level Monitoring Reagent Level Detect monitors reagent levels and alerts you when the reagent is running low, or when the waste container is full (when using the waste container with level detect).

  • Page 33: Zeroing The Reagent Level Sensors

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Zeroing the Reagent Level Sensors The Level Detect function must be zeroed at initial setup, when the stainer is moved, or if the level detect is not reporting correctly. If zeroing does not correct the problem, recalibrate the Level Detect function (Section 7.3).
  • Page 34 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Remove all reagent bottles and press Start. The display shows: NOTE: Vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. After zeroing, press OK.

  • Page 35: Controlling And Customizing Stainer Functions

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Many software settings can be controlled from the System Setup menu, including: • Creating, editing, and deleting stain programs • Creating, editing, and deleting cytocentrifuge programs • Tracking reagent information •...

  • Page 36: Adjusting The Decolorizer Level

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Adjusting the Decolorizer Level: From Adjust Settings menu, select Decolorizer. Select the desired decolorizer level (1-9). • Select the desired fixation level. • Select the desired crystal violet level. •...

  • Page 37: Managing User Access

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Enter a numeric passcode (at least 4 numbers) for the user account. Press Enter. 10 Re-enter the same passcode to confirm. 11 Press Enter. Managing User Access On the Manage Users screen, the Administrator has several options to manage user access to the instrument.

  • Page 38: Using Reagent Information Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Using Reagent Information Tracking You can enter reagent information to help track reagent usage and expiration. Reagent information includes reference number, expiration date, lot number, date and time the reagent was last installed.

  • Page 39: Modifying Level Detect Functions

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Modifying Level Detect Functions The Level Detect function alerts you when the reagent is running low, or when the waste container is almost full. You can turn reagent and waste container monitoring ON and OFF from the System Setup screen.

  • Page 40: System Log

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu System Log The instrument records all login, logout, stain or cytocentrifuge cycles, setting changes, maintenance functions and specimen identification (if enabled). Accessing Logs From System Setup, press System Log. Use navigation arrows to scroll through the log.

  • Page 41: Qc/Maintenance Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu QC/Maintenance Tracking Under system default settings, The following QC/Maintenance Tracking options are disabled: • Enable Stain Slide Tracking • Enable Cyto Slide Tracking • Enable Manual Entry • Enable Preventive Maintenance Tracking •...

  • Page 42: Enable Cyto Slide Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Enable Cyto Slide Tracking Allows slide tracking in cytocentrifuge mode. See the Cytopro Applications Manual for complete information. Enable Manual Entry If selected, allows manual entry of slide information using the keypad (limited to 24 characters).

  • Page 43: Restoring Software Defaults

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Restoring Software Defaults From System Setup, select Restore Defaults. CAUTION: Restoring the system defaults will remove all personal settings. • Restoring System Settings will delete all user names and passwords as well as all stain and cytocentrifuge programs.

  • Page 44: Recording Specimen And Reagent Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Slides with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Stain Slide Tracking. NOTE: Selecting Enable Stain Slide Tracking changes the Start button on the Main menu to ”Load Slides.”...

  • Page 45: Scanning Reagent Bottles With The Barcode Reader

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Reagent Bottles with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Reagent Tracking for each desired reagent (A, B, C, E). Press Back to return to the System Setup menu. Press Reagents to reveal the Reagent Information screen.

  • Page 46: Manually Entering Specimen Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Reagent Bottles with the Barcode Reader (continued) Press Save Repeat steps 3-8 for each reagent bottle that is enabled in QC Maintenance Tracking. NOTE: You can access Reagent Information by pressing the bottle icons on the right side of the Main menu.

  • Page 47: The Help Menu

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.3 The Help Menu The Help menu is a comprehensive onscreen help function that provides detailed information on the following subjects: Basic Operation • Loading the Carousel • Correct Reagents and Locations • Nozzle Volumes •...

  • Page 48: Operating The Aerospray Gram

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Suggested Staining Protocol • Hub Pattern Test (once per day). • If slide tracking is enabled, scan or enter slide information. • Load slides into the carousel. Use blocking slides if needed. •...

  • Page 49: Loading The Carousel

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Loading the Carousel CAUTION: Never load chipped or cracked slides into the instrument. Slides in poor condition may break during the staining cycle. If a slide breaks in the bowl, refer to Cleaning Broken Slides, Section 5.4.
  • Page 50 SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Loading the Carousel (continued) • If using a 12-slide carousel, load slides with the labels toward the outer rim of the carousel. • If using a 30-slide carousel, load slides with the labels toward the center of the carousel.

  • Page 51: Using Blocking Slides

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Using Blocking Slides If the carousel is not full, blank slides should be used as blocking slides. Blocking slides prevent overspray of reagents onto the specimen slides. Overspray can cause slides to become over-decolorized.

  • Page 52: Unloading The Carousel

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Performing a Stain Cycle (continued) If you have created a stain program, and it appears on the display, proceed to Step 4. If the desired program does not appear on the display, select Programs.

  • Page 53: Monitoring Reagent And Waste Levels

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions Monitoring Reagent and Waste Levels If enabled, the stainer displays the approximate reagent and waste container levels and other information. CAUTION: You must monitor the reagent and waste container levels on the display (if enabled) and by direct inspection of the bottles.

  • Page 54: Replacing A Reagent Bottle

    SECTION 4 OPERATING THE AEROSPRAY GRAM 4.1 Operating Instructions NOTE: You can access the Reagent Information menu by pressing the bottle icons on the right side of the Main menu. Press Change to scan or manually enter reagent information. NOTE: Do not put residual reagent from a used bottle into a new bottle. This can lead to an accumulation of residue on the slides and may be a source of contamination.

  • Page 55: Preventive Maintenance And Safety

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance The system provides a Preventive Maintenance Log for tracking the most recent maintenance activities. See Enable Preventive Maintenance Tracking in Section 3 and Using the Preventive Maintenance Log (Section 5.1) . Daily Maintenance/Quality Control (QC) Check reagent levels and expiration dates.

  • Page 56: Monthly Maintenance

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance Monthly Maintenance Disassemble and manually clean all nozzles. Refer to Nozzle Disassembly and Cleaning (Section 6.1). Disinfect the Reagent D bottle (Section 5.5). Ensure the maintenance procedures Preventive Maintenance (PM) Log have been performed, and entered into the PM chart or log.

  • Page 57: Storing The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.2 Storing the Instrument If the instrument is inactive for more than one week, you may want to perform the long-term storage procedure. This will prevent nozzles from clogging when the machine is reactivated. Preparing for Long-Term Storage With the carousel removed, remove and clean the nozzles.

  • Page 58: Replacing Fuses

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.3 Replacing Fuses WARNING! To prevent the risk of fire, the main fuses should only be replaced with fuses of the same type and rating. Recurring fuse failure indicates serious internal problems, if this occurs, contact Elitech Group.

  • Page 59: Cleaning The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.4 Cleaning the Stainer and Carousels WARNING! All cleaning procedures should be performed in a well-ventilated room by authorized trained personnel wearing appropriate protection equipment. Clean the outside of the instrument with alcohol. Clean the carousel and lid with alcohol. NOTE: Freshly prepared (<...

  • Page 60: Decontaminating The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels All parts of the instrument that come into contact with biological specimens, patient specimens, positive control specimens, or hazardous material must be treated as potentially infectious. Before the instrument is returned for service, all outer surfaces must be decontaminated. The operating authority must complete a disinfection declaration, otherwise the instrument may be rejected by the distributor or service center or quarantined by customs authorities.

  • Page 61: Decontaminating The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Figure 13: Lid Latch and Locking Pin Hole Locations 1 – Lid Latch Hole 2 – Locking Pin Hole Decontaminating the Instrument Prepare a suitable container for all disposables. Cover the lid latch and locking-pin holes with waterproof tape to protect the interior (Figure 13).

  • Page 62: Decontaminating The Reagent D Bottle

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Decontaminating the Reagent D Bottle Fill the reagent D (deionized or distilled water) bottle with a fresh (< 24 hours old) 10% bleach solution. Allow the bleach solution to react in the bottle for 10 minutes. Rinse the bottle thoroughly with tap water.

  • Page 63: Shipping Or Disposing Of The Stainer Or Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.6 Shipping or Disposing of the Stainer or Carousels Shipping the Instrument WARNING! You must disinfect the carousel before returning it to Elitech Group. The operating authority must complete a disinfection declaration, otherwise the distributor or service center may not accept the instrument;...

  • Page 64: Nozzle Maintenance And Performance

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.1 Nozzle Disassembly and Cleaning Nozzle maintenance requires the Nozzle Maintenance Kit and prepared Nozzle Cleaning Solution (diluted SS-029C). WARNING! Always wear protective clothing and eye protection when using Nozzle Cleaning Solution (diluted SS-029C). Dispose of used solution properly. NOTE: If the compression screw cannot be easily loosened, use light penetrating oil and a 5/8-in.

  • Page 65: Nozzle Reassembly

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.2 Nozzle Reassembly Hold your thumb or a strainer over the end of the tube to keep the nozzle parts in the tube. Pour out the liquid used to clean the nozzle parts. Swirl Cone Inspect the nozzle parts.

  • Page 66: Manual Priming

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.3 Manual Priming Remove the carousel from the bowl. Remove the nozzle connected to the line to be manually primed. Insert the priming tool nozzle adapter (included in the Nozzle Maintenance Kit) into the nozzle holder. Turn the nozzle adapter clockwise to install the adapter into the holder.

  • Page 67: Performing The Volume Test

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.4 Performing the Volume Test The Volume Test requires the Nozzle Maintenance Kit. NOTE: The Volume Test must be performed weekly. From the Maintenance menu, select Volume Test. Hold a Volume Test tube (small tube) in place so that selected nozzle is covered.
  • Page 68 SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.4 Performing the Volume Test Prepare the Maintenance Kit for future use: • Empty the contents of the tubes into the stainer bowl. • Rinse the tubes with water. • Put the tubes back into their original place in the Maintenance Kit or tube stand.

  • Page 69: Flushing The B-Line

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.5 Flushing the B-Line This procedure may be performed when troubleshooting a staining issue. This procedure requires the Nozzle Maintenance kit. NOTE: This procedure requires a minimum of 1 hour. NOTE: A carousel must be in place during the procedure or the instrument will generate an error and abort the procedure.

  • Page 70: Performing The Slide Pattern Test

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.6 Performing the Slide Pattern Test This test can differentiate poor staining results from sample preparation problems, or nozzle obstructions. Perform the Slide Pattern test when a Hub Pattern test produces a normal result, but staining is still inadequate.

  • Page 71: Solving Problems

    SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting This section helps you identify and solve routine problems with the stainer. More difficult problems may require technical service. Contact your Elitech Group representative for assistance. WARNING! Due to the electrical shock hazard, do not open this instrument or attempt internal repairs. Refer servicing to qualified service personnel.
  • Page 72 S E C T I O N 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 13: General Troubleshooting and Diagnosis (continued) Problem Solution Stain is leaking onto the counter. Check all external reagent lines for visible signs of cracks or loose fittings. Make sure the drain outlet is not blocked.
  • Page 73 S E C T I O N 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 13: General Troubleshooting and Diagnosis (continued) Problem Solution The stainer fails to spray reagent during a staining cycle To allow programmed staining of partial loads, the and/or continues to run after the cycle should be stainer monitors the position of the carousel as it rotates complete.
  • Page 74 S E C T I O N 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 13: General Troubleshooting and Diagnosis (continued) Problem Solution Abnormal staining on entire surface of all slides When staining a full carousel (7 or more slides for the 12- (continued).
  • Page 75 S E C T I O N 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 13: General Troubleshooting and Diagnosis (continued) Problem Solution Specimens are washing off slides (continued). If using Normal fixation, switch to High to apply more alcohol. Check the alcohol (Reagent E) level in the bottle or on the display.

  • Page 76: Abnormal Staining Results

    S E C T I O N 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Smear Separation or Cell Loss Possible causes for losing smears from the slide surface during a staining cycle: • Improper fixation • Wet smears • Thick smears •...

  • Page 77: Instrument Malfunction

    S E C T I O N 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Air or Reagent Leaks Repriming the instrument is usually unnecessary unless a reagent bottle runs completely dry. An air leak is usually to blame if a smooth and continuous liquid spray fails to come from the nozzles.

  • Page 78: Reagent Level Detect System Errors

    S E C T I O N 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Reagent Level Detect System Errors Reagent A-E Not Calibrated During the second part of calibration, if no bottles are detected, the display shows an error message. Calibrate again, making sure that the reagent bottles are inserted in those tray positions that have been enabled in the level detection system.

  • Page 79: Calibrating The Reagent Level Detect System

    SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Calibrating the Reagent Level Detect System If the Reagent Level Detect System is reporting incorrectly and zeroing (Section 2.2) does not correct the problem, calibrate the system as follows: Press System Information from the Main menu. Press System Setup.
  • Page 80 SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Calibrating the Reagent Level Detect System (continued) Remove all reagent bottles and press Start. The display shows: NOTE: Any vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. NOTE: Calibration requires full, unopened (caps and seals in place) 500 mL bottles of reagent, placed in the correct tray positions (due to different densities of each reagent type).

  • Page 81: Calibrating The Touchscreen

    S E C T I O N 7 SOLVING PROBLEMS 7.4 Calibrating the Touchscreen Select and hold Standby/Ready for 5 seconds. A calibration screen with a target appears. Select the center of the target with a finger, stylus, or similar tool. Another target will appear in a different location.

  • Page 82: Customer Service

    Elitech Group’s Service Department will help you resolve any questions about the operation or performance of your Aerospray Stainer/Cytocentrifuge. Customers in the United States should contact us by telephone. Outside the U.S., our authorized dealers offer full local service and support. ELITECHGROUP INC. 370 West 1700 South Logan, Utah 84321-8212 USA Telephone: 800 453 2725 (United States &...

  • Page 83: Cytopro® Cytocentrifuge

    S E C T I O N 8 CYTOPRO® CYTOCENTRIFUGE 8.1 Cytopro Cytocentrifuge Information Functional Description The Cytopro Cytocentrifuge rotor allows rapid sedimentation of specimen cells onto microscope slides for staining or other purposes. Up to eight disposable/reusable sample chamber assemblies with absorbent pads and glass microscope slides can be loaded into the Cytocentrifuge rotor.

  • Page 84: Appendix A: Critical Reagent Components

    APPENDIX Critical Reagent Components The following information identifies the critical chemicals of each reagent used in this instrument. Reagent(s) Critical Components SS-041A and SS-041A-EU, Gram Reagent A Decolorizer 55-65% Isopropyl Alcohol with Safranine contains: 35-45% Methyl Alcohol <1% Safranine SS-041AF and SS-041AF-EU, Gram Reagent A Decolorizer 55-65% Isopropyl Alcohol with Fuchsin contains: 35-45% Methyl Alcohol...
  • Page 85 APPENDIX Critical Reagent Components Reagent(s) Critical Components SS-141B Gram Iodine Concentrate contains: 60-70% Deionized Water 5-10% Potassium Iodide 2.5-5% Iodine SS-041C and SS-041C-EU, Gram Reagent C, Crystal Violet 95-99% Deionized water contains: 0.1-0.2% Crystal Violet SS-141C Gram Crystal Violet Concentrate when diluted as 95-99% Deionized water directed contains: 0.1-0.2% Crystal Violet...

  • Page 86: Appendix B: Risk And Safety Phrases

    APPENDIX Risk and Safety Phrases Reagents SS-041AA and SS-041AA-EU, Gram Reagent A Decolorizer with Acetone and Safranine and SS-141A Gram Reagent A Safranine Concentrate when diluted with isopropanol and acetone as directed are associated with the following Risk and Safety Phrases. European Symbols of Danger are: F and Xi. R 11: Highly flammable R 36:...
  • Page 87 APPENDIX Risk and Safety Phrases SS-041AF and SS-041AF-EU, Gram Reagent A Decolorizer with Fuchsin and SS-141AF Gram Reagent A Fuchsin Concentrate when diluted with isopropanol and methanol as directed are associated with the following Risk and Safety Phrases. European Symbols of danger are: F and T. R 11: Highly Flammable R 36:...
  • Page 88 APPENDIX Risk and Safety Phrases Reagents SS-141AF Gram Reagent A Fuchsin Concentrate is associated with the following Risk and Safety Phrases. European Symbol of Danger is: T. R 45: May cause cancer S 45 In case of accident or if you feel unwell seek medical advice immediately (show the label where possible) S 53 In case of accident or if you feel unwell seek medical advice immediately (show the...
  • Page 89 APPENDIX Risk and Safety Phrases SS-133 Decontamination Solution Concentrate is associated with the following Risk and Safety Phrases. European Symbols of Danger is Xi. R 36/38 Irritating to eyes and skin S 60 This material and its container must be disposed of as hazardous waste SS-MeOH Aerospray Methanol is associated with the following Risk and Safety Phrases.

  • Page 90: Appendix C: Accessories And Supplies

    APPENDIX Accessories and Supplies Only replacement parts supplied by Elitech Group should be used in this instrument. Use of non- approved parts may affect the performance and safety features of this product. ACCESSORIES REFERENCE NUMBER Slide Carousel (12-Slide Capacity) ............AC-188 Slide Carousel (30-Slide Capacity) ............AC-189 Nozzle Tool ....................AC-034 Nozzle Hex Wrench ..................AC-035...
  • Page 91 APPENDIX Accessories and Supplies SUPPLIES (continued) REFERENCE NUMBER Preventive Maintenance Chart, pad of 24 sheets ........SS-125 Reagent A (Safranine) Concentrate, 210 mL (Dilutes to 4.7 L) ....SS-141A Reagent A (Fuchsin) Concentrate, 135 mL (Dilutes to 4.6 L) ....SS-141AF Reagent B (Iodine) Concentrate, 500 mL (Dilutes to 5.0 L) ......SS-141B Reagent C (Crystal Violet) Concentrate, 135 mL (Dilutes to 4.6 L) ...SS-141C Contact Elitech Group for a complete list of replacement parts.

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