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Allsheng AMR-100A Operation Manual

Microplate reader
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版本修改记录
版本号
修改说明
修改日期
修改人
V4.0
2020.05.15
更改名称，更改性能指标，更新图片
周亮

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  • Page 1 版本修改记录 版本号 修改说明 修改日期 修改人 V4.0 2020.05.15 更改名称，更改性能指标，更新图片 周亮...
  • Page 2 Operation Manual Version 4.0 AMR-100 Microplate Reader Hangzhou Allsheng Instruments Co.Ltd.
  • Page 3 Please check the instruments as well as all accessories with packing list when you first open it. If you find any wrong or missing, please contact distributor or manufacturer. HANGZHOU ALLSHENG INSTRUMENTS CO., LTD. Address: Building 1 & 2, Zheheng Science Park, Zhuantang Town, Xihu District,...

  • Page 4: Safety Warning And Guidelines

    Safety warning and guidelines 1. Important information for safe use Users should have a clear main idea on how to use this instrument before operate, do read this user manual carefully. Any improper operation without reading manual is forbidden, otherwise there will be risks in cause accidental injury or electrical shock.
  • Page 5 The operator should not open or repair the Instrument by himself, which will result in losing the qualification of repair guarantee or occur accident. If there is some wrong with the Instrument, please contact manufacturer for repair. Before power on, guarantee the voltage used should be accordant to the voltage needed, and the rated load of electrical outlet should not lower than the demand.

  • Page 6: Table Of Contents

    Contents Foreword..............................I Safety warning and guidelines.......................II 1. Important information for safe use................... II 2. Safety Tips............................II Contents..............................IV Chapter 1 Brief introduction........................1 Chapter 2 Features..........................2 Chapter 3 Instrument structure......................3 Chapter 4 Installation..........................5 1. Opening check..........................5 2. Installation.............................5 3. Installation steps..........................5 Chapter 5 Operation guide........................6 Section 1 Instrument self checking....................6 Section 2 protocol interface......................
  • Page 7 2.3 Kinetics measurement Curve..................32 Section 4 Settings..........................34 1. Language setting.......................34 2. Filter setting........................35 3. Maintenance........................36 4. Print setting........................37 5. Time and Date setting...................... 37 6. Help............................. 39 Chapter 6 Maintenance, storage, transportation................40 1. Maintenance........................40 2. Storage and transportation....................42 Chapter 7 Trouble shooting.........................

  • Page 8: Chapter 1 Brief Introduction

    AMR-100 Microplate Reader Operation Manual Chapter 1 Brief introduction Chapter 1 Brief introduction This microplate reader AMR-100 is professional instrument for ELISA test , measuring concentration, absorbance, positive or negative of the antibody and antigen in the sample by testing the color of the Enzyme - Linked Immunosorbent Assay ( ELISA ). This reader is widely used in clinical test, biology agriculture, food and environment research, especially benefit from ELISA kits increasingly wide utilization.

  • Page 9: Chapter 2 Features

    AMR-100 Microplate Reader Operation Manual Chapter 2 Features Chapter 2 Features Working conditions： Ambient temperature: 4C~45C The relative humidity: 10%80% Power: AC100-240V 50-60Hz 2A The basic parameters and characteristics: Model AMR-100 Parameter Light source 6V 10W Quartz-Halogen lamp Wavelength 340～750nm Optical filter Four standard filter 405/450/492/630nm, max load eight filters.

  • Page 10: Chapter 3 Instrument Structure

    AMR-100 Microplate Reader Operation Manual Chapter 3 Instrument structure Chapter 3 Instrument structure Before first use of this instrument, please read this chapter carefully to make a better preparation. Structure: Rear cover Touch screen Roof cover Decoration bar Rear cover Front door Lower cover Plate holder...
  • Page 11 AMR-100 Microplate Reader Operation Manual Chapter 3 Instrument structure External button: “Start”, “Stop” and “Plate in/out”. Plate holder: Hold microplate. Print Port: The port is only used to connect the printer. USB Port: the port can be used to connect U disk and mouse. COM Port: connect PC.

  • Page 12: Chapter 4 Installation

    AMR-100 Microplate Reader Operation Manual Chapter 4 Installation Chapter 4 Installation 1. Opening check Each AMR-100 is completely checked before package, please check again when you receive instrument after transportation, contact your local distributor or manufacturer in case of: The outer package inverted or deformation. The outer package has an obvious stains of water.

  • Page 13: Chapter 5 Operation Guide

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide Chapter 5 Operation guide Section 1 Instrument self checking This chapter introduces default protocol operation, it begins to self checking after turned on. Refer to the picture below fig 1 There are 4 parts in the software: protocol, Report, Settings, Help. You can swift these 4parts by clicking icon area upside.

  • Page 14: Section 2 Protocol Interface

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide Section 2 protocol interface After self-check complete, enter the software main “protocol” interface. File management Parameter setting Function Key fig 2 1. File management, Function Key and Operation 1.1. File management ...
  • Page 15 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 3 Save the current protocol  Click “Save” button to save the setting protocol and view it in “File List”. Note: The new protocol will not be set after detecting sample and display in gray font; on the contrary, it still can be set.
  • Page 16 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 4 Click “Batch OP” to enter the interface(Fig 5): fig 5 You can batch deletion, import and export the protocol files. :Delete the protocol file(Fig 7). ： Click “USB Drive” button to exchange the protocol files between hard disk drive and external USB drive(Fig 6,8).
  • Page 17 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide ：Export the hard disk drive data to external USB drive. ：Import the external USB drive data to hard disk drive. ： Select the whole protocol files in the hard disk drive (external USB drive) and then the button will be change to “...

  • Page 18: Function Button

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 7 fig 8 1.2. Function button ：Click to detect the sample. ：Click to stop the detection. : Click to control the Elisa plate in and out. Note: It will create a new file when click “Run” button and then input the name of protocol file.

  • Page 19: Procedure

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide 1.3. Procedure Sample detecting procedure:  Open the procedure file (or New) and set the parameter.  Click the“Plate in/out” button on the screen or the front panel to put the Elisa plate into the instrument as Fig 9.

  • Page 20: Protocol Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 11 2. Protocol setting This part introduces how to create protocol, set parameters(wavelength, shaking mode), layout, shaking setting, data calculate, qualitative setting, quality control setting and protocol save. 2.1. Mode setting（Measure） Wavelength and mode can be set in measurement mode.
  • Page 21 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  Filter1 Setting Click right part near “Filter1” to choose wavelength(Fig 9). fig 13 There are 4 standard filter equipped: 405nm, 450nm, 492nm, 630nm, choose one as Filter1’s wavelength.  Filter2 Setting This instruments can measure with single wavelength as well as double wavelength.

  • Page 22: Layout

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  Measurement speed choice: Two mode available : Fast, Normal. Click right part near ”Mode” to choose , see picture below: fig 15 Note: “Normal” mode is recommended .  Press “OK” to finish setting and return back to main interface. 2.2.
  • Page 23 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  How to set layout: Firstly choose a type needed (the one be selected will be marked with white frame, like picture above UD(unknown sample) Type, then select well positions on plate layout (all positions are correspondent between plate layout and microplate) , the selected positions on layout will show the color of the type.
  • Page 24 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 18 You can set 9 QA control positions, and can set several wells with same number. : Unknown sample position. Set several position as unknown position. Instrument default setting all positions on plate as unknown sample. : Without sample position, the correspondent positions on microplate are without sample, the measurement result showing EP rather than data.

  • Page 25: Standard Sample Concentration Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide 2.2.2. Standard sample concentration setting On interface “Plate layout”, press “Concentration” at the right side, you will come to concentration setting interface as below(Fig 19): fig 19 It will show all 12 standard samples on this interface, only set the concentration for position ...

  • Page 26: Shake Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide curve fitting and measurement result.  “Unit” can set/select by pressing button of ”Unit” at right side(Fig 21). fig 21  Press “OK” to confirm and return back to main interface. 2.3.
  • Page 27 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 23 ” to set speed,  Click button right beside “Speed Slow, Normal, Fast(Fig 24). fig 24  Click button right beside “Time” to set time, input numbers to set hour/minute/ second(Fig 25).

  • Page 28: Calculate

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 25  There are two options “First” and “Each” in the “Mode” setting, “First” means only shake in the first testing, “Each” means every test will shake before testing. Click "Pause" button to set the waiting time after every shaking which is ahead of testing the ...
  • Page 29 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  Click button right beside “Readings”, a little keyboard for inputting measurement times.  Click button right beside “Interval” to set interval of each reading, time format: hour/minute/second.  At right side of interface , click “Parameters” to set(Fig 27). fig 27 Type: There are different Results in report indicating Rate of change, time to change, absorbance rate, due to different types you choose.

  • Page 30: Pre-Process Setting (Double Wavelength)

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 28 2.4.2. Pre-process setting (double wavelength) In the interface of “Calculate parameters”, click button right beside “Preprocess”, interface comes out as below(Fig 29): fig 29 You can choose the preprocess method when testing by double wavelength as needed. There are : M1-M2,M1/M2,M1+M2,M1*M2,M2-M1,M2/M1 (M1,M2 means absorbance value for two wavelength).

  • Page 31: Curves Fitting Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide 2.4.3. Curves fitting setting In the interface “Calculate parameters” , click button right beside “Curves”, interface comes out as below(Fig 30): fig 30 Here, you can set curving fitting by different options: linear, logistic, CubicSpline, PtoP, Factor. Choose one type needed, after measurement, software will make curve fitting by sample absorbance value data and input concentration.

  • Page 32: Quality Controls Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  Click button right beside “source”, choose one from ”ABS” and con.(for concentration) as     ， the “ a,b,c” are reagent needed. Formula for threshold value “cutoff”: control coefficient, can be set as needed, “NC” is negative control average value, “PC” is positive control average value.
  • Page 33 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 33  Click button “On” to set quality controls function by choosing Open or Close.  Click “Target” column date to set Quality controls concentration or ABS value, click SD column date to set the standard deviation value for QC concentration or ABS.

  • Page 34: Section 3 Report

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide Section 3 Report Report interface is for showing you measurement result as well as the history data of latest measurement. After measurement in interface protocol, software will go to “Report” automatically, showing the current protocol measurement result.

  • Page 35: Interpret Analysis Data

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 35 End point method export interface fig 36 Dynamics data export interface Note: Check the USB flash disk has been inserted in the instrument before export data. Click “Print” button to print the data when connect the thermal printer(Optional). 1.2 Interpret analysis data Click to show the current interpret measurement result.

  • Page 36: Analysis

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 37 Symbol above each ABS means positive, : positive, : weak positive. “ ” “ ” “ ” “ ” Right side shows threshold value “Cutoff”, refer part 2.5 for details(Fig 37).、 Note: The print result “P”...

  • Page 37: Curves

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 38 1.4 Curves Standard curve fitting result with formula, you can preprocess the ABS and input standard concentration and then make curve fitting if the previous fitting is not good enough(Fig 39). How to preprocess: Linear/Linear ( Linear the ABS and corresponding concentration), Linear/Log(Linear the ABS and corresponding concentration log value), Log/Linear, Log/Log, Log/Linear.

  • Page 38: Kinetics Report Interface

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 40 2. Kinetics Report interface 2.1 Kinetics measurement Raw data fig 41 The picture above(Fig 41) showing the ABS value under the current measurement cycle, click to shift the cycles. indicates the cycle no., click this button and input cycle no.

  • Page 39: Kinetics Measurement Analysis

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide 2.2 Kinetics measurement Analysis fig 42 indicating the data process method of kinetics measurement(Fig 42). (See section 2, 2.4.1 Kinetics setting) 2.3 Kinetics measurement Curve fig 43 It indicates change curve of each sample position ABS detection according to different cycles under kinetics measurement(Fig 43).
  • Page 40 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide Press to shift sample position on microplate, when at A1 and click to see absorbance change curve all of sample positions(Fig 44). fig 44 ─ ─...

  • Page 41: Section 4 Settings

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide Section 4 Settings This part mainly introduces settings of Language, filter, maintenance, print, quality control, screen calibration, date and time. Click Setting at the upper are of the main interface: fig 45 1.

  • Page 42: Filter Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 47 Select one language needed and press OK to complete. Now only English and Chinese are available. 2. Filter setting This instrument is equipped with four standard filters which have positions on the filter wheel from 1-4.

  • Page 43: Maintenance

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide  lick the blank position No., input the wavelength, default unit is nm(Fig 49). fig 49 In case of wrong input, double click to input again. Standard equipped with 1-4 filter, and the wavelength are as picture above.

  • Page 44: Print Setting

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide control microplate holder position in or out of the instrument. Click “Keep  in” button to choose “Keep in” or “Keep out” . “Keep in” will make microplate holder in side after measurement, while “Keep out”...
  • Page 45 AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide fig 52 In case that you want to change date and time, click the area to set(Fig 53), fig 53 Click “Enter” to complete setting. ─ ─...

  • Page 46: Help

    AMR-100 Microplate Reader Operation Manual Chapter 5 Operation guide 6. Help Brief introduction every function button as fig 54 below. fig 54 Click “Protocol” enter the function introduce interface. Click “Results” enter the result introduce interface. Click “Settings” enter the set introduce interface. Click “...

  • Page 47: Chapter 6 Maintenance, Storage, Transportation

    AMR-100 Microplate Reader Operation Manual Chapter 6 Maintenance, storage, transportation Chapter 6 Maintenance, storage, transportation 1. Maintenance Keep storage environment dry and clean to prevent moisture, corrosion, away from strong  electromagnetic interference sources. Instrument already calibrated before leave factory. User is not allowed to disassembly and ...
  • Page 48 AMR-100 Microplate Reader Operation Manual Chapter 6 Maintenance, storage, transportation fig 56 2) Install optical filter Instrument is equipped with four standard filter inside, in case of need other wavelength, please operate as following steps, turn off the instrument before changing filter. Loose the knob to open the back door, take out the optical filter wheel by the equipped allen wrench(Fig 57).

  • Page 49: Storage And Transportation

    AMR-100 Microplate Reader Operation Manual Chapter 6 Maintenance, storage, transportation fig 58 Note: Instrument is equipped with four standard filter inside, please ignore this step if you don’t need  other filters. Other filters besides standard ones need to be purchased separately. ...

  • Page 50: Chapter 7 Trouble Shooting

    AMR-100 Microplate Reader Operation Manual Chapter 7 Trouble shooting Chapter 7 Trouble shooting Fault phenomenon Possible Causes Solutions Check power supply, Check cable plug status, Instrument can not start Power defective Check fuse, Check voltage Power to lamp failure, Check power before replace, Lamp not on Lamp defective Replace a lamp...

  • Page 51: Chapter 8 Accessories

    AMR-100 Microplate Reader Operation Manual Chapter 8 Accessories Chapter 8 Accessories Item Specs Unit Remark Power cord Touch pen Mouse Logitech Cross screwdriver SATA 62307 Install or remove filter Install or remove filter Allen wrench 2.5mm wheel Certification Operation Manual Performance test statement Packing List...
  • Page 52 Memo ─ ─...

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