Shodex F6110002 Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex IEC CM-825 is a weak cation exchange column filled
with hydrophilic polymer-based gel modified with carboxymethyl group. It is an ideal column for analyzing
various proteins, peptides, and components of nucleic acids as it has a low adsorption tendency towards
hydrophobic compounds. The polymer-based gel allows its use at a wide pH range condition.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
IEC CM-825
F6110002
Base Material : Spherical porous particles of polyhydroxymethacrylate modified with carboxymethyl group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : 50 mM Sodium Sulfate aq. Solution
4. Usable Conditions
Product Name
IEC CM-825
Usable solvents are listed below.
(1) The standard eluents are buffers and aqueous solutions of salt such as sodium chloride, potassium chloride,
sodium sulfate, and potassium sulfate. Salts may also be added to the buffers. The recommended total salt
concentration ranges are 20 mM to 1 M. Select a buffer which has negative charge ion and has a large
buffering capacity at the desired pH. A list of usable buffers is given below.
Operation Manual
Shodex IEC CM-825
Column Size (mm)
I.D. Length
8.0
Flow Rate (mL/min)
Recommended Maximum
1.0
Resonac Corporation (https://www.shodex.com/)
Particle Theoretical Plate
Size
(μm)
75 8
Maximum
Pressure
(MPa/column)
1.5 2.0
ver.20221202E
Ion Exchange
Number Capacity
(Per Column) (meq/g)
≥ 2,000
Temperature
pH Range
Range (°C)
2 - 12 10 - 50
0.40
1