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DOT eDNA Sampler Operation And Maintenance Manual

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DOT eDNA Sampler
Operation and Maintenance Manual
Document No: DOT-OM-0002-12
Prepared by:
Dartmouth Ocean Technologies Inc.
www.DartmouthOcean.com
|
Aftersales@DartmouthOcean.com
|
(902) 406-0700

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  • Page 1 DOT eDNA Sampler Operation and Maintenance Manual Document No: DOT-OM-0002-12 Prepared by: Dartmouth Ocean Technologies Inc. www.DartmouthOcean.com Aftersales@DartmouthOcean.com (902) 406-0700...

  • Page 2: Revision History

    Revision History Rev. Release Date Prepared Approved Revision Description 2024 Feb. Updated with Firmware Version 3.12 2024 Oct. Updated for Firmware Version 3.16 Hardware Version 3.05 Graphical User Interface 3.01 Includes Deep-Rated features Minor Text Updates Shallow and Deep Energy Calculations...

  • Page 3: Table Of Contents

    Table of Contents Safety & Specifications ....................5 Safety Information ......................5 Identification ........................6 Specifications ........................7 Parts & Equipment ......................9 Physical Setup ....................... 11 System Description ......................11 Assembly Instructions ....................... 15 3.2.1 Installation and Removal of Reagent Reservoirs ..............15 3.2.2 Removing and Installing Filter Membranes ................
  • Page 4 Figure 7: Loosening shoulder bolts for removal of Reagent Case. (Right) Necessary gap shown....19 Figure 8: Reagent Case rotation direction relative to eDNA Sampler Body to disengage slots....20 Figure 9: eDNA Sampler with Reagent Case removed to expose reagent face plate ......... 20 Figure 10: Fluid reservoir installation using the IDEX Fitting Wrench and Luer valve.
  • Page 5 List of Tables Table 1: Mechanical specifications ....................... 7 Table 2: Electrical specifications ........................7 Table 3: Fluids specifications (in field) ......................7 Table 4: Standard filtration specifications ....................8 Table 5: Manufacturer-supplied equipment....................9 Table 6: Required user-supplied equipment ....................10 Table 7: Miscellaneous equipment (sold-separately) .................

  • Page 6: Safety & Specifications

    Spills or leaks should be immediately cleaned with a wipe while wearing the required PPE. There are four (4) different fluids contained in the reagent case of the DOT eDNA Sampler: 1) RNAlater Preservative – labelled R1 2) Milli-Q Water (high purity deionized water) – labelled R2 3) Cleaning Solution (5% Hydrochloric acid, HCl) –...

  • Page 7: Identification

    Refer to these numbers when communicating with DOT concerning warranty, servicing, or technical issues. Figure 1: Model, Serial Number (S/N), and Part Number (P/N) shown on side of Sampler.

  • Page 8: Specifications

    Sampler Operation Manual (0002-12) Safety & Specifications Specifications Table 1: Mechanical specifications Parameter Specification Maximum Depth Shallow-Rated Up to 20 m Deep-Rated Up to 3000 m Operating Temperature Range 4 °C – 35 °C Weight (Shallow-Rated) In Air (full reagent reservoirs) 12.7 kg (27.9 lbs)

  • Page 9: Table 4: Standard Filtration Specifications

    Filters that abort due to clogging will still be preserved with RNAlater. This version of the eDNA Sampler uses a Filter Cassette loaded with reusable Filter Holders. Filter Holders support filters of 25 mm diameter. The thickness / material type of the Filter Membrane, however, must properly fit in the Filter Holder such that the Filter Holder may properly seal.

  • Page 10: Parts & Equipment

    Sampler Operation Manual (0002-12) Parts & Equipment 2 Parts & Equipment The following table lists the components supplied with each DOT eDNA Sampler Kit (Part No. 1196): Table 5: Manufacturer-supplied equipment Part no. Description DOT-1123 eDNA Sampler (Shallow-Rated) DOT-1548...

  • Page 11: Table 6: Required User-Supplied Equipment

    [Recommended] Large lint-free wipes (e.g Kimwipes™) PPE – Lab Goggles, Safety Glasses, Long-sleeved clothing [Recommended] IPA (Isopropyl Alcohol) The following table lists the additional components that can be supplied by DOT at Aftersales@dartmouthocean.com: Table 7: Miscellaneous equipment (sold-separately) Part no.

  • Page 12: Physical Setup

    Filter Membranes. Filters loaded with eDNA are automatically preserved in situ for later analysis. To operate the eDNA Sampler, a Graphical User Interface (GUI) is supplied with the instrument. The sampler has been designed for easy use by a single operator: •...

  • Page 13: Figure 2: Edna Sampler With Labelled Sections (Green Boxes) And Components (Black Boxes)

    Sampler Operation Manual (0002-12) Physical Setup Figure 2: eDNA Sampler with labelled sections (green boxes) and components (black boxes) Table 8: List of major system sections and components in Figure 2 Section / Component Description Sampler Body Performs major sampler functions (electronics, logging, automation).
  • Page 14 The protocol outlined above requires three (3) fluids; 5% HCl solution, RNAlater, and Milli-Q water. Each fluid is contained in a flexible bag located in the eDNA Sampler Reagent Case. The spent RNAlater is collected in an onboard waste reservoir, also found in the Reagent Case.
  • Page 15 Sampler Operation Manual (0002-12) Physical Setup b. A pre-programmed sampling condition occurs, telling the Sampler to acquire an environmental DNA sample. This occurs automatically when automated sampling is enabled, but requires the instrument to be powered. In the GUI, the Sampler will respond “SLEEP” to user commands when in sleep mode. To exit sleep mode:...

  • Page 16: Assembly Instructions

    The DOT Reagent Kit contains the fluid reservoirs needed for Sampler operation. Each kit contains four (4) fluid reservoirs that must be connected to the eDNA Sampler before use. The kit includes three (3) flexible plastic reservoirs filled with chemical reagent and one (1) reservoir used to collect the sample preservation waste.

  • Page 17: Table 9: Fluid Port Connections And Fluid Volumes

    Physical Setup There is a corresponding connection fitting for each of the four (4) reservoirs on the eDNA Sampler End Cap, referred to as “fluid ports”. Each port on the end cap is engraved with a label that matches the reservoir label, R1, R2, R3, and R4.

  • Page 18: Figure 4: Fluid Bags With Shutoff Valve & Cap. Never Remove Black Cap Or Purple Luer Shutoff Valve

    Sampler Operation Manual (0002-12) Physical Setup Figure 4: Fluid bags with Shutoff Valve & Cap. Never remove black cap or purple Luer Shutoff Valve. WARNING Always wear the required PPE when removing or installing reagent reservoirs. Wipe up all fluid leaks and spills immediately.

  • Page 19: Figure 5: Tools Required For A Fluid Bag Change

    Sampler Operation Manual (0002-12) Physical Setup Installation of New Fluid Reservoirs Required Tools / Equipment • (PPE) Protective gloves / clothing / eye and face protection • eDNA Reagent Kit with four (4) new fluid reservoirs • IDEX Fitting Wrench •...

  • Page 20: Figure 6: Edna Sampler On Cradles, With Tools

    Sampler Operation Manual (0002-12) Physical Setup Figure 6: eDNA Sampler on cradles, with Tools. 3) Loosen the three (3) shoulder bolts that secure the Reagent Case to the Sampler body using the 4 mm -handle Allen Key. The bolts should be rotated a minimum of six (6) full turns to ensure that the head of the bolt is clear of the counterbore in Reagent Case.

  • Page 21: Figure 8: Reagent Case Rotation Direction Relative To Edna Sampler Body To Disengage Slots

    Reagent Case. Figure 8: Reagent Case rotation direction relative to eDNA Sampler Body to disengage slots. 5) Pull Reagent Case and Cradle back from the Sampler to expose the four (4) fluid connections and the cable connections on the Sampler reagent face plate.

  • Page 22: Figure 10: Fluid Reservoir Installation Using The Idex Fitting Wrench And Luer Valve

    Sampler Operation Manual (0002-12) Physical Setup 7) If Y-Splitter is not previously installed: Unscrew the locking collar from the 8-pin female dummy plug on the Sampler reagent face plate and unplug dummy plug from the bulkhead connector. Store dummy plug and locking collar with Tool / Spares Kit.

  • Page 23: Figure 11: Reagent Case And Sampler Body After Installation Of Y-Splitter And Reagent Reservoirs

    Sampler Operation Manual (0002-12) Physical Setup Figure 11: Reagent Case and Sampler Body after installation of Y-Splitter and Reagent reservoirs 13) Re-engage Reagent Case into Sampler Body. The Reagent Case is indexed to the Sampler Body and will engage in one (1) orientation only. There are alignment marks on the Reagent Case and Sampler Body to facilitate correct alignment.

  • Page 24: Figure 12: Reagent Case With End Cap Removed

    Sampler Operation Manual (0002-12) Physical Setup Figure 12: Reagent Case with End Cap removed Dartmouth Ocean Technologies Inc.
  • Page 25 Sampler Operation Manual (0002-12) Physical Setup Removal of Depleted Reagents / Filled Waste Reservoirs Required Tools / Equipment • (PPE) Protective gloves / clothing / eye and face protection • Four (4) Luer fluid Port Caps (disconnecting fluids for long term sampler storage) •...

  • Page 26: Figure 13: Luer Caps Installed In All Four (4) Fluid Ports For Long Term Storage

    Sampler Operation Manual (0002-12) Physical Setup 11) Install red sealing caps found in Tools / Spares Kit on fluid ports in reagent face plate for each of the four (4) fluid ports. Figure 13: Luer Caps installed in all four (4) Fluid Ports for long term storage.

  • Page 27: Removing And Installing Filter Membranes

    Sampler Operation Manual (0002-12) Physical Setup 3.2.2 Removing and Installing Filter Membranes CAUTION Always wear clean gloves when handling the Filter Cassette, Filter Holders, and Filter Membranes. Use sterile tools to remove Filter Membranes; DO NOT handle filters by hand.

  • Page 28: Figure 15: Filter Holder Disassembly With Labelled Components

    Sampler Operation Manual (0002-12) Physical Setup Installation of Filter Membranes in a Clean Filter Holder Required Tools and Equipment • Cleaned filter handling tools (i.e. tweezers wiped with 70% ethanol, disposable gloves, clean working surface) • Up to nine (9) clean Filter Holders •...

  • Page 29: Figure 16: Flow Direction Of Sample Water Through Filter Holders

    Sampler Operation Manual (0002-12) Physical Setup 3) Using sterilized equipment, place Filter Membrane in recessed portion of Filter Holder base. NOTE: If using Filter Membranes that require a specific flow direction through the membrane, refer to Figure 16 for direction of flow.
  • Page 30 Sampler Operation Manual (0002-12) Physical Setup Removal of Filter Membrane from Filter Holder Required Tools and Equipment • Sterile filter handling tools (i.e. tweezers wiped with 70% ethanol, disposable gloves, clean working surface) • Lint-free wipes • Nine (9) Filter Membrane storage containers to catalogue samples To remove the Filter Membrane sample from the Filter Holder, perform the following procedure: 1) Wear required PPE when interacting with filters and filter holders: disposable lab gloves.

  • Page 31: Figure 17: Assembling The Cassette Holder

    Dirty Filter Holders without Filter Membranes may be installed on a Filter Cassette for automated cleaning with the eDNA Sampler. Cleaned Filter Holders with NEW Filter Membranes may be installed on a cleaned Filter Cassette prior to eDNA Sampler deployment.

  • Page 32: Figure 18: Filter Cassette Installed In Cassette Holder

    Sampler Operation Manual (0002-12) Physical Setup Figure 18: Filter Cassette installed in Cassette Holder Figure 19: Filter Holder partially engaged in Cassette. 7) Screw Filter Holder into Filter Cassette using the black Actuation Knob on the opposite side of the Cassette.

  • Page 33: Figure 20: Filter Holder Fully Engaged In Filter Cassette; Filter Holder Is Bottomed Out On O-Ring

    Sampler Operation Manual (0002-12) Physical Setup Figure 20: Filter Holder fully engaged in Filter Cassette; Filter Holder is bottomed out on O-ring. 8) When installation of all nine (9) Filter Holders is complete, remove Filter Cassette from the Cassette Holder. Orient Cassette such that the side containing the Filter Holders is clearly visible to technician.
  • Page 34 Sampler Operation Manual (0002-12) Physical Setup Removal of Filter Holders from Filter Cassette Required Tools and Equipment • Clean working surface • Stubby 3/4”wrench • Cassette Holder • PPE – Disposable Gloves, Safety Glasses The following steps detail the procedure required to remove Filter Holders from the Filter Cassette: 1) Assemble Cassette Holder and place on working surface.

  • Page 35: Installing, Replacing, & Storing Filter Cassette

    The eDNA Filter Cassette provides a quick and easy way to change out and store the nine (9) filter samples processed by the DOT eDNA Sampler. The DOT eDNA Sampler Kit is sold with one (1) Filter Cassette that comes installed on the Sampler. Additional Filter Cassette Shipping Kits can be purchased to facilitate the collection of additional samples in the field.

  • Page 36: Figure 22: Edna Sampler With Removed Filter Cassette

    • Sampler Body Cradle (labelled in storage case) NOTE: The various fluid pathways of the eDNA Sampler must be cleaned before installing a fresh Filter Cassette with Membrane Filters to prevent cross-contamination from previous use of the eDNA Sampler. Refer to Section 5.3 Internal Cleaning...

  • Page 37: Figure 23: Edna Sampler With Attached Filter Cassette

    It is important to follow Procedures 9) and 10) carefully. These steps ensure that the Filter Cassette is fully engaged onto the Sampler and that all eighteen (18) O-ring seals are properly mated. Figure 23: eDNA Sampler with attached Filter Cassette Dartmouth Ocean Technologies Inc.

  • Page 38: Figure 24: Removing Filter Cassette From Sampler Body

    • Filter Cassette Kit (for storage of processed Filter Cassette) To remove a Filter Cassette from the eDNA Sampler, perform the following procedure: 1) Put on PPE (safety glasses and fresh disposable lab gloves). 2) Locate a clean horizontal surface and position Sampler into the Sampler Body Cradle and the Reagent Case Cradle.

  • Page 39: Figure 25: Filter Cassette On Cassette Sealing Plate

    Sampler Operation Manual (0002-12) Physical Setup 5) Immediately turn the Filter Cassette face down, in order to prevent possible sample contamination through fluid dripping onto the Filter Holders. Place Filter Cassette face down on large, clean lint free wipe.

  • Page 40: Maintenance Of Inlet And Outlet Ports

    Sampler Operation Manual (0002-12) Physical Setup 3.2.4 Maintenance of Inlet and Outlet Ports The Sampler Inlet Port and the Outlet Port are two identical assemblies – see Figure 26. They each employ two (2) filters: a coarse mesh copper screen and a finer mesh PEEK filter. The copper screen requires periodic cleaning, and the PEEK filter requires periodic replacement.

  • Page 41: Figure 26: Installed Inlet Filter

    Sampler Operation Manual (0002-12) Physical Setup Replacement & Cleaning of Inlet or Outlet Port Filters Required Tools / Equipment • Clean working surface & lab gloves • Inlet Filter Pin Wrench • T-handle Allen Key • 3/4” Stubby Wrench •...

  • Page 42: Figure 28: Removing Inlet Filter Housing Using Pin Wrench And ¾" Stubby Wrench

    Sampler Operation Manual (0002-12) Physical Setup Figure 28: Removing Inlet Filter Housing using Pin Wrench and ¾” Stubby Wrench. 2) Clean Filter Housing thoroughly to remove marine growth prior to disassembly. Exercise care in cleaning around captured O-ring. A soft (plastic) bristled brush is recommended to prevent damage to O-ring.

  • Page 43: Figure 30: Membrane Plate Removal, Exposing Peek Filter

    Sampler Operation Manual (0002-12) Physical Setup 4) Clean the Membrane Plate thoroughly with IPA using a soft bristled brush. Visually inspect captured O-ring for damage after cleaning. Replace O-ring as required when the O-ring is visibly deformed or damaged. Apply O-ring grease to O-rings before reassembly.

  • Page 44: Figure 32: Inlet Filter Assembly Steps 1 & 2 Shown From Left To Right

    Sampler Operation Manual (0002-12) Physical Setup To assemble the Inlet Filter or Outlet Filter Housing, perform the following procedure: 1) Install Copper Screen, Retaining Ring, and new PEEK Filter into metallic half of Inlet Housing. 2) Install Membrane Plate into Housing with O-ring facing PEEK filter.

  • Page 45: Figure 34: Filter Final Assembly (Step 3) With T-Handle Allen Key

    Sampler Operation Manual (0002-12) Physical Setup Figure 34: Filter final assembly (Step 3) with T-handle Allen Key WARNING DO NOT overtighten the four (4) Inlet Port Housing screws. Excessive tightness can result in damage to holes in plastic Cover Plate.

  • Page 46: Electrical & Software

    There are two (2) electrical connections on the sampler: an 8-Pin male bulkhead connector, used for power and communications; and a 6-pin male bulkhead connector, used for programming by DOT. The 6-pin male bulkhead connector is plugged with a dummy plug and not intended for customer use.
  • Page 47 Pull plug and locking sleeve from 6-Pin (M) cable to expose pins. c. Store dummy plug and locking sleeve. 4) Connect the 6-Pin (F) of the DOT eDNA 24V Power Adapter to the 6-Pin (M) power cable of the Y- splitter.
  • Page 48 • Y-splitter The DOT Battery Pack can be used to power the eDNA Sampler when an external power supply is not available. Battery Packs can be used on the bench use or in field deployments. Refer to Appendix C: Energy Calculations to estimate battery lifetime.
  • Page 49 4) Plug USB cable from DOT Control Pendant into an available PC USB port. 5) Open DOT GUI application to communicate with Sampler. Sampler might be in ‘sleep mode’ on initial connection; if this is the case power cycle the sampler by disconnecting from power, waiting 5 seconds, and reconnecting to power.

  • Page 50: Sampler Programming

    Warnings or error messages (if applicable) As described previously, an application is provided by DOT to operate the eDNA Sampler using a Graphical User Interface (GUI). For users that wish to operate the eDNA Sampler through an external controller without use of the GUI, contact DOT through email at Aftersales@DartmouthOcean.com...

  • Page 51: Graphical User Interface (Gui) Installation

    4.2.2 Graphical User Interface (GUI) Installation The DOT eDNA Sampler Graphical User Interface (GUI) is an application provided by DOT to operate the eDNA Sampler. Upon installation of the application, the user must restart their PC before use. Be sure to save &...

  • Page 52: Sampler Operation

    5 Sampler Operation This Section describes the various information and steps needed to operate the eDNA Sampler. A Graphical User Interface (GUI) application is provided to configure the eDNA Sampler – refer to Section Electrical & Software for download & installation instructions. For further information on operating the GUI, refer to the eDNA Sampler GUI Operation Manual.

  • Page 53: Sleep Mode

    Sampler Operation Manual (0002-12) Sampler Operation Sleep Mode For Shallow-Rated eDNA Samplers, your instrument will enter a low power state referred to as “sleep mode” after five (5) minutes of inactivity. In sleep mode, the sampler will not respond to user commands, and will only “wake up”...

  • Page 54: Consumables

    Sampler Operation Manual (0002-12) Sampler Operation Consumables It is critical that the various consumables used during Sampler Operation are monitored and replaced when necessary. Using the GUI, a check is performed upon instrument connection on current reservoir fluid volumes and sample inlet usage levels. A warning may be issued to the user is displayed when any of these consumables are too low to sample one full cassette with a cleaning cycle.

  • Page 55: Reagents

    The final row also displays the total fluid volume consumption for three full cassettes with necessary cleaning and priming. These values should be compared to the current fluid levels of the eDNA Sampler to determine when a new set of fluids is needed.

  • Page 56: Updating Consumables In Graphical User Interface (Gui)

    GUI when levels are low. It is up to the user to update consumables levels. When consumables are removed and/or installed, the user must update the eDNA Sampler via the GUI to reflect these changes; otherwise, fluid tracking will not be accurate and sample captures may be compromised.

  • Page 57: Internal Cleaning & Priming Instrument

    Sampler Operation Manual (0002-12) Sampler Operation Internal Cleaning & Priming Instrument A full system (internal) clean is needed when installing new Filter Cassettes to prevent contamination from previous use. A system clean is also recommended for instruments that have not been used for an extended period.
  • Page 58 Required Tools / Equipment • DOT Control Pendant • Communications cable • Laptop / PC with DOT GUI program installed • Inlet / Outlet port hose fittings, with attached tubing • Waste Container • PPE: Disposable gloves, lab coat, safety glasses...
  • Page 59 Communications cable • DOT Control Pendant • Inlet / Outlet port hose fittings, with attached tubing • Powered eDNA Sampler with Cassette and sufficient fluids for cleaning • Waste Container • Fluid Collection Tray • PPE: Disposable gloves, lab coat, safety glasses This Section describes the procedure to clean the internal lines of the Sampler Body without a Filter Cassette.

  • Page 60: Figure 37: Edna Sampler (Right) With Fluid Collection Tray (Left)

    Sampler Operation Manual (0002-12) Sampler Operation Figure 37: eDNA Sampler (right) with Fluid Collection Tray (left) Cleaning Sampler with Sampler held vertically a. Locate fluid collection tray. Place on a flat surface with plenty of room. b. Mount Sampler vertically such that the three threaded posts coming from the exposed cassette face slide into the three holes of the collection tray.
  • Page 61 Sampler Operation Manual (0002-12) Sampler Operation 4) If applicable, remove Sampler from fluid collection tray and place on a flat horizontal surface using cradles. Install fresh Filter Cassette loaded with clean Filter Holders and new Filter Membranes. Click “New Cassette”...
  • Page 62 Sampler Operation Manual (0002-12) Sampler Operation Clean Filter Holders and Filter Cassette in Lab Required Tools / Equipment • Watertight plastic container with cover, large enough to hold Cassette and Filter Holders • Supply of 5% HCl • Milli-Q water •...

  • Page 63: Configure Automated Sampling

    Sampler Operation Manual (0002-12) Sampler Operation Configure Automated Sampling The Automated Sampling tab is used to configure automated sample scheduling. There are two main modes of automated sampling: Trigger by Time, and Trigger by Depth (Deep-Rated units only). There are three important variables used to govern Automated Sampling: •...

  • Page 64: Trigger By Time

    Trigger by Time Users can schedule a date-time “trigger” for each of the nine (9) filters to define a full sampling schedule as shown in Figure 39. Figure 39: Automated Sampling tab in DOT GUI [Trigger by Time] Dartmouth Ocean Technologies Inc.

  • Page 65: Trigger By Depth (Deep-Rated Units Only)

    Filter 2 is active. Filter 2 will then begin when the Sampler is pulled back up, as it will pass through the applicable region. Users must therefore exercise caution when setting depth triggers. Figure 40: Automated Sampling tab in DOT GUI [Trigger by Depth] Dartmouth Ocean Technologies Inc.

  • Page 66: Scheduling Automated Sample Captures

    NOTE: If a sample is underway and the next date-time condition is met, that second sample will be skipped to finish the current sample. DOT recommends a minimum interval of 1 hour between samples to avoid skipped samples; however, this is dependent on the targeted Sampling volume.

  • Page 67: Manual Sampling

    Sampler Operation Manual Sampling Sample captures can be immediately activated using the Manual Sampling tab of the DOT GUI, shown in Figure 41. Manual Sampling uses the same sample capture protocol as Automated Sampling and can be reviewed under Status – Protocols. An...

  • Page 68: Sampler Deployment

    Review consumable levels under Status – Instruments in the DOT GUI. The eDNA Sampler does not have to be retrieved immediately after completion of the sampling operation. However, the following limitations should be considered during longer-term deployments: 1) Preservative: the manufacturer of the RNAlater preservative guarantees the following •...
  • Page 69 Sampler Operation Manual (0002-12) Sampler Deployment • Samples can be stored at +4 °C for one month, • Samples can be stored at +25 °C for one week, 2) Biofouling and clogging of inlet and outlet ports; dependant on environmental factors.

  • Page 70: Sampler Retrieval

    Reservoirs. Clean sampler end cap and internal cavity of reagent case thoroughly. Rinse with fresh water. 4) Connect to Sampler through the DOT GUI application. Check status of consumables, download data, and update consumables levels consumables have been serviced. 5) Remove processed Filter Cassette from Sampler Body and install in Cassette Sealing Plate. Refer to Section 3.2.3 Installing, Replacing, &...
  • Page 71 Up to nine (9) new Filter Membranes • PPE: Disposable gloves To redeploy the eDNA Sampler, perform the following procedures after the steps detailed in Initial Retrieval from the Environment: 6) Upon completion of the automated cleaning operation, remove Cassette from Sampler and install fresh Filter membranes in each Filter Holder.
  • Page 72 The sampler is now ready for long term storage. WARNING The eDNA sampler is stored with Milli-Q in all the fluid transfer lines. Freezing temperatures will cause serious damage. Always ensure that instrument is stored in a heated environment above +10°C.

  • Page 73: Maintenance & Care

    6) Use a silicone-based lubricant on the pins and in the sockets. Apply lubricant to the sockets by putting a small quantity on your finger and pushing as much as possible into each of the sockets. DOT recommended lubricant, supplied in Tool/Spares Kit: •...

  • Page 74: Anode Replacement

    Between deployments, inspect the Sampler Body for scratches / corrosion. The eDNA Sampler body employs two sacrificial anodes, each located on opposite sides. These anodes are made of Zinc and will corrode more readily than aluminum. As they corrode, they will shrink in size from loss of mass.

  • Page 75: Warranty

    Warranty Any personalized warranty agreement issued between DOT and the client overrules the following general agreement. The contents of this section apply only if no other warranty agreement between DOT and the customer has been issued. Dartmouth Ocean Technologies Inc. warrants that the DOT eDNA Sampler will be free from defects of workmanship and materials for a period of one (1) year.

  • Page 76: Appendices

    Sampler Operation Manual (0002-12) Appendix A: Mechanical Drawings 8 Appendices Appendix A: Mechanical Drawings Dartmouth Ocean Technologies Inc.
  • Page 77 Sampler Operation Manual (0002-12) Appendix A: Mechanical Drawings Dartmouth Ocean Technologies Inc.
  • Page 78 Sampler Operation Manual (0002-12) Appendix A: Mechanical Drawings Dartmouth Ocean Technologies Inc.
  • Page 79 Sampler Operation Manual (0002-12) Appendix A: Mechanical Drawings Dartmouth Ocean Technologies Inc.
  • Page 80 Sampler Operation Manual (0002-12) Appendix A: Mechanical Drawings Dartmouth Ocean Technologies Inc.

  • Page 81: Appendix B: Electrical Pinouts

    Sampler Operation Manual (0002-12) Appendix B: Electrical Pinouts Appendix B: Electrical Pinouts Table 12: Sampler power and comms pinout: 8-pin male (From Sampler) Function Face View (Male) Ground Ground 9 – 24 VDC 9 – 24 VDC Ground RS232 RX IN...

  • Page 82: Appendix C: Energy Calculations [Shallow-Rated Units]

    ���������� 0.030 �� One Cassette can be sampled over the course of a 519-day period with a fully charged DOT Battery Pack. CASE III: Max deployment length to sample three (3) Cassettes powered by one (1) DOT Battery Pack (1.5 hours per sample): 561.6 Wh −...
  • Page 83 = 373 ℎ�� = ���� �������� ���������� 1 �� One Cassette can be sampled over the course of a 19-day period with a fully charged DOT Battery Pack. CASE III: DOT Battery Pack: Max Number of Samples Without Delay (1.5 hours per sample): 561.6 Wh = ( 0.8 ) ∗...

  • Page 84: Appendix D: Refilling Reagent Reservoirs

    Sampler model. • To avoid contamination, DOT recommends use of a peristaltic pump with a separate set of tubing for each fluid. If a peristaltic pump is not available, a large syringe will work but is much more time consuming, and additional care must be taken to avoid contamination.
  • Page 85 Sampler Operation Manual (0002-12) Appendix E: Refilling Reagent Reservoirs Refilling 5% Hydrochloric Acid Bag 1) Empty remaining acid into waste container 2) Inject approximately 30 mL of 5% hydrochloric acid into the bag using the port with the purple Luer lock valve.

This manual is also suitable for:

Dot-1123Dot-1548Dot-1549

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