Olympus IX3-DSU Instructions Manual
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Olympus IX3-DSU Instructions Manual

Olympus IX3-DSU Instructions Manual

Disk scanning unit for ix3
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INSTRUCTIONS
IX3-DSU
DISK SCANNING UNIT FOR IX3
This instruction manual is for the Olympus Disk scanning unit for IX3 model IX3-DSU.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of the
disk scanning unit for IX3, we recommend that you study this manual thoroughly before operating
the microscope. In addition, be also sure to read the instruction manual for the microscope on which
the disk scanning unit for IX3 is installed.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
- Research use only -
A X 8 3 0 5

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Summary of Contents for Olympus IX3-DSU

  • Page 1 IX3-DSU DISK SCANNING UNIT FOR IX3 This instruction manual is for the Olympus Disk scanning unit for IX3 model IX3-DSU. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of the disk scanning unit for IX3, we recommend that you study this manual thoroughly before operating the microscope.
  • Page 2 Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in EU for return and/or collection systems available in your country. For Korea only A급...

  • Page 3: Table Of Contents

    CONTENTS IMPORTANT -- Be sure to read this section for safe use of the equipment. -- CAUTIONS ....................................1 Releasing the Transport Lock ............................1 SAFETY PRECAUTIONS ............................2 Symbol for safety considerations .............................3 Warning labels ......................................3 When using the system ..............................4 Cleanup and storage ................................5 Caution ......................................5 Manual conventions ................................5 1 SYSTEM OUTLINE ...........................
  • Page 4 3-11 Disk IN/OUT ..................................22 3-12 Power shutdown ...............................22 4 VARIETIES OF OBSERVATION METHODS ..............Fluorescent Observation ..................................23 Transmitted Bright Field Observation and Transmitted Phase Contrast Observation ....23 4-1 Visual observation ..............................23 4-2 Observation with Camera ..........................23 5 TROUBLESHOOTING GUIDE ....................6 SPECTRAL CHARACTERISTICS OF FILTERS ............
  • Page 5 9-11 Disk installation and change .........................42 9-12 Attaching the Anti-Glare Plate ........................43 9-13 Attaching the Shutter (IX2-SHA) ........................43 9-14 Attaching the spacer .............................43 9-15 Field iris diaphragm IN/OUT ..........................44 9-16 Attaching the camera adapter ........................44 9-17 Attaching the Camera ............................45 9-18 Attaching the Motor Drive Board ........................46 10 LAMP HOUSING INSPECTION SHEET ..............

  • Page 7: Cautions

    IX3-DSU IMPORTANT CAUTIONS This manual – part or whole, shall not be used or reproduced without permission. Releasing the Transport Lock Be sure to work on the following tasks first after finishing the unpacking. When powering up the control box without removing the CAUTION transportation lock knob, the disk unit may be damaged.

  • Page 8: Safety Precautions

    · This device complies with the emission and immunity requirements described in IEC61326 series. · Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER CORD” at the end of this instruction manual. If the proper power cord is not used, the safety and EMC performance of the device can not be assured.

  • Page 9: Symbol For Safety Considerations

    IX3-DSU Symbol for safety considerations This system comes with the following symbols. Understand the meaning of the symbols and handle it safely. Symbol Meaning Be careful so as not to entangle your finger with disk or mirror unit for camera observation.

  • Page 10: When Using The System

    · Do not use the instruments where they are subjected to direct sunlight, high temperature and humidity, dust or vibrations. · The fluorescent observation by vision is not available with IX3-DSU, If you perform the observation by vision, use the IX3 system for fluorescent observation separately.

  • Page 11: Cleanup And Storage

    IX3-DSU Cleanup and storage · Never put dirt or fingerprint on the surface of lens and filter, etc. In case of contamination, use gauze and wipe out lightly. In case that the dirt or fingerprint does not come off with gauze, then, use mixed liquid (alcohol 3: ether 7) to put in gauze and wipe out gently.

  • Page 12: System Outline

    I. System Outline...

  • Page 13: Principle

    IX3-DSU SYSTEM OUTLINE Attaching this system to a microscope, it comprises a confocal microscope system without laser, but with use of a white light source. It acquires a high contrast image through resolution enhanced in light axis direction very rapidly.

  • Page 14: Features

    1-2 Features · Confocal disk employed enables to acquire image of confocal effect. · As while light source such as mercury or xenon is used as a light source instead of laser, excited light of various wavelengths can be used by combination of excitation filter and cube.

  • Page 15: System Configuration

    Z drift compensator IX3-ZDC Shutter Control box IX2-SHA IX3-CBH Cooled CCD Control box Light source camera IX2-UCB U-HGLGPS Filter wheel (with IX3-DSU) Touch panel controller Controller U-MCZ Shutter interface IX2-IFSHA DSU hand switch (with IX3-DSU) C-mount adapter Hand switch DSU-CAD U-HSTR2...
  • Page 16 Configuration of Instruction Manuals Instruction manual title Module name Research inverted microscope IX83 Control box IX2-UCB Hand switch U-HSTR2 Shutter interface IX2-IFSHA Reflected fluorescence system IX3-RFACA, IX3-RFACS, IX3-RFA etc. Instruction manual supplied with your Cooled CCD camera Cooled CCD camera Instruction manual supplied with your computer Computer/monitor...
  • Page 17 II. DSU...

  • Page 18: Summary Of Observation Procedures

    SUMMARY OF OBSERVATION PROCEDURES This section describes the procedures for performing the fluorescent observation with vision by using your reflected fluorescent system and the observation with camera and the confocal observation by using the DSU system continuously. For the fluorescent observation with vision, refer to the instruction manual of [Reflected Fluorescent System] provided separately. Also, for operations of the microscope IX83, refer to the instruction manual of [IX83] together with the above.
  • Page 19 IX3-DSU Adjust the camera settings to match with the Control of Camera — observation. If necessary, insert/remove the Filter selection button ND filter into/from the light path and adjust the light intensity. Field stop lever 3-10 Adjust the field stop.

  • Page 20: Details Of Each Operation

    DETAILS OF EACH OPERATION 3-1 General Precaution for observation 1. Verify that the input voltage/frequency to be used is within the range of that described on the identification plate of the system. 2. Verify that the power cord as well as the connecting cord is securely connected.

  • Page 21: Selection Of Fluorescent Mirror Unit

    IX3-DSU 3-2 Selection of fluorescent mirror unit Exclusively for Fluorescent Proteins Excitation Mirror Unit Dichroic Mirror Excitation Filter Barrier Filter Fluorochrome Method U-MCFPHQ DM450HQ BP425-445HQ BA460-510HQ ECFP use DSU-MGFP* DM485HQ BP460-480HQ BA495-540HQ EGFP use U-MYFPHQ DM505HQ BP490-500HQ BA515-560HQ EYFP use...

  • Page 22: Table Of Combination - Objective Lens To Each Observation And Applicable Disk

    3-3 Table of Combination – Objective lens to each observation and applicable disk Reflected Fluorescence Differential Objective Applicable disk interference DSU-D1 ○ ○ ○ ○ ○ ○ ○ － UPLSAPO DSU-D2 DSU-D1 ○ ○ ○ ○ ○ ○ ○ ○ 10x2 DSU-D2 DSU-D1...
  • Page 23 IX3-DSU Reflected Fluorescence Differential Objective Applicable disk interference DSU-D1 △ ○ ○ ○ ○ ○ ○ ○ PLAPON 1.25x DSU-D3 DSU-D1 △ ○ ○ ○ ○ ○ ○ ○ DSU-D2 DSU-D1 △ ○ ○ ○ ○ ○ ○ ○ 60xO...
  • Page 24 Reflected Fluorescence Differential Objective Applicable disk interference DSU-D4 ○ ○ ○ ○ ○ ○ ○ ○ UAPON 100xOTIRF DSU-D4 DSU-D4 ○ ○ ○ ○ ○ ○ ○ ○ 150xOTIRF DSU-D4 DSU-D3 － ○ ○ ○ ○ ○ ○ ○ LUMFLN 60xW DSU-D4 DSU-D1...

  • Page 25: Powering Up

    IX3-DSU 3-4 Powering up 1. Switch on the control box. 2. Switch on the lamp. 3. Switch on CCD camera. } For detail procedures for turning on the powers of other systems, refer to the instruction manual of IX83 provided separately.

  • Page 26: Selecting Mirror Unit Built-In Dsu

    3-7 Selecting Mirror Unit built-in DSU Press the button of the hand switch to rotate the mirror unit, and engage the desired mirror unit into the light path. In the factory default setting, the button to rotate the mirror unit is assigned to the buttons (1) and (2) shown in the picture on the left.

  • Page 27: Shutter Open/Close

    IX3-DSU 3-9 Shutter OPEN/CLOSE The shutter can be opened or closed from U-HSTR2 by either of procedures described below. } Press the button (1) of U-HSTR2 to open or close the shutter IX2- SHA. } Press the button (2) of U-HSTR2 to set to the No.6 filter wheel.

  • Page 28: Disk In/Out

    3-11 Disk IN/OUT Press the button on the left side (IN) (1) of the DSU hand switch to engage the disk into the light path. And press the button on the right side (OUT) (2) of the DSU hand switch to remove the disk from the light path.

  • Page 29: Varieties Of Observation Methods

    IX3-DSU VARIETIES OF OBSERVATION METHODS 4-1 Visual observation } For detail procedures of the visual observation, refer to the instruction manual of [IX83] or [Reflected Fluorescent System] provided separately. When changing the light path by setting the tube side to 50% and the camera side to 50%, the extremely CAUTION bright light may enter your eyes.

  • Page 30: Troubleshooting Guide

    Depending upon how the system is used, it may not exhibit adequate performance of the system, though it may not be a failure. When trouble occurs, exercise adequate countermeasure with reference to the following table. In case that trouble has not been improved, please contact local Olympus representative for assistance. Trouble...
  • Page 31 IX3-DSU Trouble Cause Countermeasures Ref. Flare is remarkable. Ambient light (such as Turn off fluorescent lamp or cut ambient light with fluorescent lamp) is entering use of specimen cover. – light path. Exposure time of camera is not Adjust to adequate time.

  • Page 32: Spectral Characteristics Of Filters

    SPECTRAL CHARACTERISTICS OF FILTERS 2. MGFP 1. CFPHQ DSU-MGFP U-MCFPHQ BP460-480GFP DM490GFP DN450 BP425-445HQ BA495-540GFP BA460-510HQ 3. YFPHQ 4. RFPHQ U-MYFPHQ U-MRFPHQ BP490-500HQ DM505 BP535-555HQ DM565HQ BA515-560HQ BA570-625HQ } DSU-MGFP and U-MRFPHQ come with this system as standard accessories.
  • Page 33 IX3-DSU Major Emission Spectra from Ultrahigh-Vacuum Mercury Burner Wavelength (nm) For fluorochrome emission, a light beam having a specific wavelength is selected from a wide range of spectra. The five major peaks of luminance are at wavelengths of 365/366, 404.7, 435, 546.1 and 577.0/579.1 nm. In addition, light beams having wavelengths of 334.2 and 490 nm (at rather low luminance) are also applicable to fluorochrome...

  • Page 34: Specifications

    · Equipped with the height adjustment mechanism · 2 units are provided. Attaching Camera C mount (1-32UNF) Software cellSens (Operates and controls IX3-DSU, IX83F and motorized parts of other motorized units) *For details, refer to the instruction manual (cellSens) provided separately. Switch Operation · Disk rotation The hand switch is provided.
  • Page 35 IX3-DSU Operating environment · Indoor use. · Altitude: Max. 2000 meters · Ambient temperature: 10 ° to 35 °C (50 ° to 95 °F) · Maximum relative humidity: 80% for temperatures up to 31 °C (88 °F) (without condensation) In case of over 31 °C (88 °F), the relative humidity is decreased linearly through 70% at 34 °C (93 °F), 60% at 37 °C (99 °F), and to 50% at 40 °C (104 °F).

  • Page 36: Adjustment

    ADJUSTMENT 8-1 Centering the Field Stop } Use the Allen screwdriver provided with the microscope to operate the centering screws. Close the shutter of the manual shutter. (See (1) in the picture.) Engage the fluorescent mirror unit of B excitation into the light path. Open the shutter of the mirror unit cassette.

  • Page 37: Centering Of The Lamp

    IX3-DSU 8-2 Centering of the Lamp } It is not necessary to perform the centering of the lamp in each observation, but perform the centering when replacing the lamp or if you recognize the uneven brightness in the acquired image.
  • Page 38 } If it is difficult to operate the collector lens focus knob (1) of the lamp house, insert the extension handle U-CLA (option) into the knob before use. } Lamp centering is not required until next lamp change. Stringent mirror centering } Position of mirror is fixed after adjustment at ex-factory.

  • Page 39: Camera Focus Alignment

    IX3-DSU 8-3 Camera focus alignment Show the live image on the monitor, engage the 10X objective lens into the light path and move the specimen out of the view field. Press the button (1) on the left side (IN) of the DSU hand switch and engage the disk into the light path.

  • Page 40: Assembly

    IX2-UCB-2 Power cord RS232C cable DSU hand switch DU-HSW Hand switch U-HSTR2 *Three ferrite core contains in the IX3-DSU. **Two ferrite core contains in the IX2-IFSHA. For detail procedures of the mounting position, refer to the instruction manual of [IX2-IFSHA].

  • Page 41: Attaching The Dsu Adapter

    IX3-DSU 9-2 Attaching the DSU adapter Loosen the clamping screw (1) of the DSU adapter by using the Allen screwdriver provided with the microscope frame, and detach the DSU adapter (2) from the disk box. Loosen the side port clamping screw (3) placed on the left side surface of the microscope frame by using the Allen screwdriver provided with the microscope frame.

  • Page 42: Attaching The Filter Wheel

    9-4 Attaching the Filter Wheel Screw the support leg (1) into the filter wheel and attach it. • Make the support leg shortest before attaching it to the disk CAUTION box. • Make sure to attach the support leg to prevent from falling. Loosen the clamping screw (3) (2 positions) of the disk box by using the Allen screwdriver provided with the microscope frame.

  • Page 43: Attaching The Lamp

    IX3-DSU 9-6 Attaching the Lamp Light guide << Applicable lamp >> DC130W SHI Lamp (USHIO INC. SHI-130 OL) Before replacing the lamp, be sure to turn \ (OFF) the power CAUTION switch and unplug the power cord from the outlet, and wait (more than 40 minutes as a guide) until the lamp replacement cover and the lamp are cooled down sufficiently.

  • Page 44: Marcury Lamp

    Marcury lamp } If your system uses the xenon burner, attach the burner, set the hour counter and connect the system by referring to the instruction manuals for the lamp housing and power supply unit. Loosen the socket clamping screw using the Allen screwdriver. Hold the upper section of the lamp housing and pull it upward to remove the socket section.

  • Page 45: Lamp Power Supply Setting

    ” (OFF) before connect- ing the lamp housing cable and power cord. • Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by refer- ring to the section “PROPER SELECTION OF THE POWER SUP- PLY CORD”...

  • Page 46: Attaching The Mirror Unit Cassette

    Insert mirror unit (3) to be used into mount dovetail (4) of the mirror unit and push it until it hits to dead end. Tighten the fixing screw (1), using the Allen screwdriver. } The mirror units used with IX3-DSU cannot be used with the inverted fluorescent system of IX83.

  • Page 47: Method To Make Optional Fluorescent Mirror Unit

    IX3-DSU 9-10 Method to make optional fluorescent mirror unit } You can make optional fluorescent mirror unit by attaching a mirror unit frame U-MF2 to commercially available barrier filter, excitation filter or dichroic mirror. Dimensional conditions for optical parts · Barrier filter Max.

  • Page 48: Disk Installation And Change

    9-11 Disk installation and change To replace the disk, turn off the mainswitch of the control box, CAUTION and wait 1 minute until the disk stops rotating. Loosen screws (2) (2 places) at front side of access panel (1) located on top of the disk box, using the Allen screwdriver and remove the access panel.

  • Page 49: Attaching The Anti-Glare Plate

    9-14 Attaching the spacer ◎ For IR observation (when using GFP cubes), be sure to attach the spacer provided with IX3-DSU in order to secure the optical perfor- mance. Loosen the fixing screws (1) (2 places) of the filter wheel by using the Allen screwdriver provided with the microscope.

  • Page 50: Field Iris Diaphragm In/Out

    9-15 Field iris diaphragm IN/OUT (Removal). Loosen fixing screw (1) located at the bottom of aperture unit, using the Allen screwdriver. Hold aperture unit (2) and pull it horizontally. (Installation). Reverse procedure When you tighten fixing screw (1), tighten it lightly. CAUTION Do not tighten it too hard.

  • Page 51: Attaching The Camera

    IX3-DSU 9-17 Attaching the Camera Loosen fixing screw (1) of port, using the Allen screwdriver that comes as accessory for microscope frame and remove C-mount adapter (2) inside the port. Screw in and fix the C-mount adapter (2) that was removed to C-mount section (3) of camera firmly.

  • Page 52: Attaching The Motor Drive Board

    9-18 Attaching the Motor Drive Board You must install motor drive board after you turn power of CAUTION control box to OFF. Loosen the four fixing knobs for the two option slot covers at the left side of the back panel of the control box (1) and remove the covers and knobs.

  • Page 53: Lamp Housing Inspection Sheet

    · If there is any ( ) mark noted, immediately stop use of the product, and contact Olympus for detailed inspections or replace the lamp housing. · If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and contact Olympus for detailed inspections.

  • Page 54: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 55 IX3-DSU Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on of color section in mm) Approval Organization jacket or insulation of internal...
  • Page 56 MEMO...
  • Page 60 Manufactured by Distributed by AX8305 01...

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