Page 1 228-90075D Jul. 2005 COLUMN OVEN FOR SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH CTO-20A/20AC INSTRUCTION MANUAL Read the instruction manual thoroughly before you use the product. Keep this instruction manual for future reference.
Page 2 This page is intentionally left blank.
Page 3 • To ensure safe operation, read the Safety Instructions before using the instrument. COPYRIGHT • Copyright (C) 2004-2005 Shimadzu Corporation. All rights reserved. This publication may not be reproduced in whole or in part without written permission from Shimadzu Corporation. Since Shimadzu products are frequently upgraded and improved, information in this publication is subject to change without notice.
Page 4: Warranty And After-Sales Service
Introduction Warranty and After-Sales Service Warranty Validity Please consult your Shimadzu representative for information about the extent of the warranty. Term The manufacturer will provide free replacement parts for, or repair free of charge, any instrument that fails during the warranty period, if the cause can be attributed to a defect in manufacturing.
Page 5 "7 Hardware Validation" P. 7-1 Hardware Validation This is a contract under which a qualified Shimadzu-approved engineer performs periodic component and system validation, and provides reports of Contract the results. Details of the contract can be obtained from your Shimadzu representative. CTO-20A/20AC...
Page 6: Safety Instructions
Application Precautions WARNING This instrument is a column oven for use with a high performance liquid chromatography system. Use this instrument ONLY for the intended purpose. Using this instrument for any other purpose could cause accidents. CTO-20A/20AC...
Page 7: Installation Site Precautions
Otherwise, the instrument could tip over or fall off the table. At least 600mm • Avoid installation sites that are exposed to corrosive gases or excessive dust. These adverse conditions may be detrimental to maintaining the instrument performance and may shorten its service life. CTO-20A/20AC...
Page 8: Installation Precautions
The cord could be damaged, resulting in fire, electrical shock or malfunction. If the cord becomes damaged, contact your Shimadzu representative immediately. • Do not modify the cord in any way. Do not bend it excessively or pull on it.
Page 9: Operation Precautions
It could break the reservoir bottles and cause injury. • Do not use flammable sprays (hair sprays, insecticide sprays, etc.) near the instrument. They could ignite and cause a fire. CTO-20A/20AC...
Page 10: Precautions For Instrument Inspection, Maintenance, Adjustment And Care
• Never remove the main cover. This may cause injury or malfunction of the instrument. The main cover does not need to be removed for routine maintenance, inspection and adjustment. Have your Shimadzu representative perform any repairs requiring removal of the main cover.
Page 11: Static Electricity Precautions
Air bubbles in liquid facilitate generation of static electricity. Insulated container of spark polyethylene, etc. Dry air Flammable gas present in container. spark Flammable organic solvent with large electrostatic charge. Floor covered with rubber, etc., cannot conduct electricity away. CTO-20A/20AC...
Page 12: Preventing Static Electricity Accidents
Connect clip to metal parts. Connect to ground terminal or other grounding point of the Reduce the opening instrument to ground metal with a cap. container. 18 Liter metal can (preferably plated) Liquid's static will be conducted through container to ground. CTO-20A/20AC...
Page 13 Use tubing with an inner diameter of at least 2mm for drain lines with high flow rates. CAUTION • Periodically check the tubing connections for leaks. Air bubbles in liquid can multiply the electrostatic charge by a factor of 20, 30 or more. CTO-20A/20AC...
Page 14: For Reference
• Use as small a container as possible to minimize damage in the event of fire. • Keep the room at a proper humidity. Ambient humidity exceeding 65% will prevent static. For Reference Anti-static equipment (anti-static clothing, shoes and matting) and charge measurement equipment (potentiometer) are sold by specialty manufacturers. CTO-20A/20AC...
Page 15: Precautions For Mobile Phase Selection And Use
• Always degas the mobile phase, as air bubbles may tend to form during solvent mixing or during temperature or pressure changes. Air bubbles may cause pump malfunctions and detector signal noise. • For boiling points, viscosities and other data relating to the mobile phases used, "9.5 Mobile Phase Characteristics" P. 9-40 CTO-20A/20AC XIII...
Page 16: Warning Labels
Introduction Warning Labels For safety operation, warning labels are affixed to where special attention is required. Should any of these labels peel off or be damaged, obtain replacements from Shimadzu Corporation. Warning label (Part No. 228-28122) CTO-20A/20AC...
Page 17: Table Of Contents
Opening and Closing the Left Door ..............2-3 2.2.1 Opening the Left Door ..................2-3 2.2.2 Closing the Left Door ..................2-4 Interior ......................2-5 Back .........................2-6 Names and Functions of Displays and Keypad ..........2-7 2.5.1 Display Panel ....................2-7 2.5.2 Keypad ......................2-8 CTO-20A/20AC Contents...
Page 18 [SET TEMP] .........................5-13 [T.MAX] ........................5-13 [EVENT] ........................5-13 [RV LEFT, RV RIGHT] ....................5-14 5.2.4 Monitor Settings Group ................. 5-15 [AMBIENT] ........................5-15 [LS LEVEL] ........................5-15 [PROG TIME] .......................5-15 [MONITOR RV] ......................5-16 5.2.5 System Settings Group ................. 5-17 [LOCAL] ........................5-17 [LINK ADRS] ........................5-17 CTO-20A/20AC Contents...
Page 19 [OP MODE] ......................... 5-32 [INITIALIZE PARAM] ....................5-32 [CHANGE PASSWORD] ..................... 5-33 Creating Time Programs ................5-34 5.4.1 Time Program Command ................5-34 5.4.2 Basics of Programming ................. 5-34 5.4.3 Time Program Flow ..................5-35 Setting a New Program ....................5-35 CTO-20A/20AC Contents...
Page 20 Troubleshooting and Corrective Action ............6-2 Error Messages ....................6-3 Chapter 7 Hardware Validation Overview of Hardware Validation ..............7-2 7.1.1 Hardware Validation ..................7-2 7.1.2 Types of Hardware Validation ................. 7-2 Implementation of Hardware Validation ............7-3 7.2.1 Periodic Validation ..................7-3 CTO-20A/20AC Contents...
Page 21 Leak Sensor Test ..................7-15 Objective ........................7-15 Items Required for Check .................... 7-15 Check Procedure ......................7-15 Temperature accuracy calibration ..............7-17 System Validation ...................7-19 7.7.1 Validation of Isocratic LC system ..............7-19 Objective ........................7-19 Items Required for Validation ..................7-19 CTO-20A/20AC Contents...
Page 22 Installation ........................9-4 Stacking Brackets ......................9-4 9.1.3 Power Supply Connection ................9-5 Connection to Power Outlet ...................9-6 Grounding ........................9-6 9.1.4 Prior to Plumbing ..................... 9-7 Types of Tubings and Connectors .................9-7 Cutting Tubings ......................9-7 Connecting Tubings .......................9-8 Protective Plugs ......................9-10 CTO-20A/20AC Contents...
Page 23 Function of Components ..................... 9-37 9.4.2 Example of Autosampler System (1) ............. 9-38 Solvent Flow ........................ 9-38 Function of Components ..................... 9-38 9.4.3 Example of Autosampler System (2) ............. 9-39 Solvent Flow ........................ 9-39 Function of Components ..................... 9-39 CTO-20A/20AC Contents...
Page 24 Contents Mobile Phase Characteristics .................9-40 Index CTO-20A/20AC Contents...
Page 25 Configuration Contents Overview ......................1-2 Features ......................1-2 Component Parts ..................... 1-3 Optional Parts ....................1-4...
Page 26: Overview
The oven is an air-circulated thermostatic chamber for the Shimadzu High Performance Liquid Chromatography system. The CTO-20A model column oven regulates temperatures with a heater only, while the CTO-20AC model is additionally equipped with cooling mechanisms that enable it to regulate the oven and flow lines to temperatures below room temperature.
Page 27: Component Parts
Column clamps (small) 228-15617-91 Column clamps (large) 228-15617-92 Drain tubing kit 228-43969-91 Ambient temperature sensor 228-34620-92 Event cable 228-28253-91 Signal cable 228-39306-91 Terminals 071-03511-01 Clamp DKN-10GSP 072-60319-01 Note CTO-20A/20AC 228-35779 Listed of auxiliary functions.Put it on Label, CTO-20A/20AC 228-46424 conspicuous place. CTO-20A/20AC...
Page 28: Optional Parts
Valve that automatically switches between up to 6 columns. FCV-14AH 228-45014-91 Two are required: one at column inlet, one at outlet. Unit for upgrading from the CTO-20A (without cooler) to the CTO-20AC Cooler addition unit 228-45700-91 (with cooler). Column clamps 228-28637-91 One column clamp can hold two 6.4-12.7mm outer diameter columns.
Page 29 Parts Identification and Function Contents Front Cover ...................... 2-2 Opening and Closing the Left Door..............2-3 Interior ......................2-5 Back ......................... 2-6 Names and Functions of Displays and Keypad ..........2-7...
Page 30: Front Cover
Drainage hole for liquid leaked inside the oven. Press in Press again (Power ON) (Power OFF) NOTE When the doors are opened, temperature regulation stops and the "oven" indicator blinks. Temperature regulation resumes as soon as the doors are closed. CTO-20A/20AC...
Page 31: Opening And Closing The Left Door
Then, push the left door in slightly while moving the lever up slowly. 2.2.1 Opening the Left Door Move the lever up, and pull the left door open. Lever Fig. 2.1 CTO-20A/20AC...
Page 32: Closing The Left Door
Move the lever back down after closing the door. This locks the left door. Down Fig. 2.3 NOTE The lock can be disabled if not required. Hand-tighten the screw shown in the figure to keep the lever in the raised position. Screw Fig. 2.4 CTO-20A/20AC...
Page 33: Interior
Fixes columns. Hold the doors closed. Thermosensor Senses temperature. Leak sensor Senses organic solvent vapors. Door lock Locks the left door. Safety switches Check that doors are properly closed. Column switching valve plates Column switching valves are installed here. CTO-20A/20AC...
Page 34: Back
Connector for ambient temperature sensor. [REC] connector Connector for ambient temperature (oven temperature) sensor output (100°C/mV). NOTE The [REC] connector is for making adjustments and normally does not need to be used. Power cord connector Connect the power cord. CTO-20A/20AC...
Page 35: Names And Functions Of Displays And Keypad
∗ If the door is opened, temperature regulation stops, and the oven operation indicator blinks. Temperature regulation resumes when the door is closed. Time program indicator prog.run On when time program is running. Remote control mode indicator remote On when the system controller is used. CTO-20A/20AC...
Page 36: Keypad
Sleep mode turns off the display screen, but operation is not affected. Scrolls forward through parameters. Each time this is pressed, the display switches to next parameter. Function key Pressing this key after selecting a VP function item switches the VP function setting screen to the next item. CTO-20A/20AC...
Page 37 Deletes individual lines of the current time program (when writing/editing Delete key time programs). Valve key For moving to the automatic column switching valve setting screen. Numeric keypad Sets the numerical values for the display screen. Enter key Registers or executes a command displayed on the screen. CTO-20A/20AC...
Page 38 2. Parts Identification and Function This page is intentionally left blank. 2-10 CTO-20A/20AC...
Page 39: Installation Procedures
Installation Procedures Contents Column Installation................... 3-2...
Page 40: Column Installation
• Attach the upper column clip to the lower row of holes the lower row of holes in the upper bracket. This prevents the column from coming into contact with the top of the cover. Fig. 3.1 Column bracket Fig. 3.2 CTO-20A/20AC...
Page 41 3.1 Column Installation Orient the column with its outlet at the top (as shown on the right), and push it into the column clips. Verify that the column is held securely. Column outlet Column Column inlet Fig. 3.3 CTO-20A/20AC...
Page 42: Installation With Column Clamps (Optional)
Fig. 3.5 Finish tightening the cover screw. Verify that the column is held securely. NOTE • Tighten and loosen the cover screw to install and remove columns. • One column clamp can hold two 6.4-12.7mm outer diameter columns. Fig. 3.6 CTO-20A/20AC...
Page 43 Basic Operation Contents Turning Power ON/OFF ................... 4-2 Setting Operating Temperature [SET TEMP]........... 4-3 Setting Upper Temperature Limit [T.MAX] ............4-4 Operation ......................4-5...
Page 44: Turning Power On/Off
• In the example screen shown here, the set SET ACTUAL T.MAX temperature is 40°C and the upper temperature limit is 90°C. XX.X • If an error is detected, an alarm sounds and an error message is displayed. "6.2 Error Messages" P. 6-3 CTO-20A/20AC...
Page 45: Setting Operating Temperature [Set Temp]
The temperature set here is displayed as the set temperature on the initial screen. This setting remains in memory when the power is turned off. Press And proceed to "4.3 Setting Upper Temperature Limit [T.MAX]". ∗ Press twice to display the initial screen. CTO-20A/20AC...
Page 46: Setting Upper Temperature Limit [T.max]
Press twice to display the initial screen. NOTE If the upper temperature limit is exceeded during temperature regulation, [ERR OVER T.MAX] appears ERR OVER T.MAX and temperature regulation is stopped. CE to Clear For corrective action, P.6-3 CTO-20A/20AC...
Page 47: Operation
The next setting screen (for upper temperature T.MAX limit) appears. Input 5 - 90 ∗ To return to the parameter setting screen, press Use the numeric keypad to set the upper temperature limit to 70°C. T.MAX · · (Press Input 5 - 90 CTO-20A/20AC...
Page 48 The manual injector handle becomes especially hot. NOTE Prevent tubing and other objects from getting through the metal grille inside the column oven. This could ERR FAN damage the fan. If the fan operates abnormally, [ERR FAN] is displayed. For corrective action, P.6-4 CTO-20A/20AC...
Page 49 Application Operation Contents Display Panel ....................5-2 Parameter in Auxiliary Functions ..............5-10 VP Functions....................5-22 Creating Time Programs ................5-34 Control by CBM-20A or CBM-20Alite System Controller ....... 5-48 Control by SCL-10Avp or SCL-10A System Controller ........5-49 Connection to External Input/Output Terminals ..........5-50...
Page 50: Application Operation
Press func or VP VP functions settings • Product Information • Maintenance Information • Validation Support • Calibration Support Time program edit screens P.5-34 Used Left Time programs editing ∗ Press to return to the initial screen from any screen. CTO-20A/20AC...
Page 51: Auxiliary Functions Screens
L E F T 1 2 A H I n p u t 0 - 1 Right automatic column switching valve setting P.5-14 R I G H T 1 4 A H I n p u t 1 - 6 Next page CTO-20A/20AC...
Page 52 L I N K A D R S I n p u t 1 - 1 2 Keypad disabling P.5-18 K E Y C L O S E E n t e r t o C l o s e Next page CTO-20A/20AC...
Page 53 0 ( A U T O ) , 4 0 0 - 1 2 0 0 Operation of Buzzer P.5-21 B E E P M O D E ™ 0 : O n 1 : A l m 2 : O f f ∗ COOLER MODE appears only on CTO-20AC. CTO-20A/20AC...
Page 54: Vp Function Screens
P r e s s f u n c o r V P L 2 0 2 1 X X X X X X X ROM version No. display P.5-25 S / W I D : V * . * * C T O - 2 0 A C Next page CTO-20A/20AC...
Page 55 E n t e r t o D i s p l a y Error log display For verification, press to scroll through entries (up to 10). P.5-27 E R R O R L O G E n t e r t o D i s p l a y Next page CTO-20A/20AC...
Page 56 E n t e r t o S t a r t Leak sensor test Press to run. P.5-30 L E A K S E N S O R T E S T I n j e c t a n d E n t e r Next page CTO-20A/20AC...
Page 57 C H A N G E P A S S W O R D E n t e r t o C h a n g e P R O D U C T I N F O P r e s s f u n c o r V P CTO-20A/20AC...
Page 58: Parameter In Auxiliary Functions
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. 5-10 CTO-20A/20AC...
Page 59: System Settings Groups
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. CTO-20A/20AC 5-11...
Page 60: Showing The Auxiliary Function Screen
Input 4 - 85 Press and select the desired parameter. Follow the directions of each parameter described in the next page. Press to move to other functions. Press to show the group screen. Press to return to the initial screen. 5-12 CTO-20A/20AC...
Page 61: Parameter Settings Group
Relay 1 OFF Relay 2 OFF Relay 1 ON Relay 2 OFF Relay 1 OFF Relay 2 ON Relay 1 ON Relay 2 ON Press to go to the next function. ∗ To return to the initial screen, press twice. CTO-20A/20AC 5-13...
Page 62: [Rv Left, Rv Right]
12AH Input 0 - 1 Enter the values 0, 1, or 1-6 by the numeric keypad, and press LEFT 14AH Input 1 - 6 Press to go to the next function. ∗ To return to the initial screen, press LEFT twice. NONE 5-14 CTO-20A/20AC...
Page 63: Monitor Settings Group
∗ If not using this function, press to go to the next one. Read the setting instructions displayed. PROG TIME after Prog Start Press to go to the next function. ∗ To return to the initial screen, press twice. CTO-20A/20AC 5-15...
Page 64: Monitor Rv
[-] (hyphens) are displayed in place of the settings. ∗ Setting [MONITOR RV] to returns the initial screen to its normal state. Press to go to the next one. ∗ To return to the initial screen, press twice. 5-16 CTO-20A/20AC...
Page 65: System Settings Group
Read the setting instructions displayed. LINK ADRS Input 1 - 12 Enter the values 1-12 by the numeric keypad, and press Press to go to the next function. ∗ To return to the initial screen, press twice. CTO-20A/20AC 5-17...
Page 66: Key Close
Read the setting instructions displayed. BRIGHTNESS Input 1 - 4 Enter the values 1-4 by the numeric keypad, and press Press to go to the next function. ∗ To return to the initial screen, press twice. 5-18 CTO-20A/20AC...
Page 67: Ext-S
If auto operation is set, the cooler will come on automatically whenever the set operating temperature changes to a relatively low value (near room temperature plus 10°C). NOTE Set Manual ON when using the oven for applications where the cooler is turned on during analysis, such as linear temperature regulation. CTO-20A/20AC 5-19...
Page 68: Ready Range
Enter the values 0-10 by the numeric keypad, and press NOTE A setting of [0] means that the oven is [READY] when it is on. Press to go to the next function. ∗ To return to the initial screen, press twice. 5-20 CTO-20A/20AC...
Page 69: Fan Speed
∗ To return to the initial screen, press twice. NOTE A setting of [1200] for the fan speed in the CTO-20A/ 20AC is roughly equivalent to a setting of [21] for the fan speed in Shimadzu's previous CTO-10A series ovens.
Page 70: Vp Functions
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. 5-22 CTO-20A/20AC...
Page 71: Validation Support Group
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. CTO-20A/20AC 5-23...
Page 72: Displaying The Vp Functions
To move to a different VP Function Group, press Press func or VP to return to the group title screen, then select the desired group again. Press repeatedly. CALIBRATION Press func or VP To return to the initial screen, press 5-24 CTO-20A/20AC...
Page 73: Product Information Group
This group provides the information about the instrument. PRODUCT INFO Press func or VP [SERIAL NUMBER] Shows the serial number of this instrument. SERIAL NUMBER L2021XXXXXXX [S/W ID] Shows the name of software (same as the model name) and version. S/W ID:V*.** CTO-20AC CTO-20A/20AC 5-25...
Page 74: Maintenance Information Group
No. 012-34567-89 was replaced on July 1, 2003. LOG 1 03-07-01 P/N:012-34567-89 If less than 10 logs are recorded, the screen displays the message as shown on the right. Press to return to the title screen. No more Logs 5-26 CTO-20A/20AC...
Page 75: Operation Log
July 1, 2003. LOG 1 03-07-01 ERR LEAK DETECT If less than 10 logs are recorded, the screen displays the message as shown on the right. Press to return to the title screen. No more Logs CTO-20A/20AC 5-27...
Page 76: Validation Support Group
Use the numeric keypad to set the month 07 (= July). DATE -MM- Then press 03-07 Use the numeric keypad to set the day 01. Then press DATE Setting is now complete. The date set will be 03-07-01 displayed. to end DATE YY-MM-DD 03-07-01 5-28 CTO-20A/20AC...
Page 77: Time
MEMORY CHECK Enter to Start Results are shown when checking is completed. Result ∗ The ROM/RAM error status will be displayed if an error MEMORY CHECK is found in the ROM or RAM. ROM OK / RAM OK CTO-20A/20AC 5-29...
Page 78: Temperature Test
5. Application Operation [TEMPERATURE TEST] "7.5 Validation : Column Oven" P. 7-7 TEMPERATURE TEST Enter to Start [LEAK SENSOR] "7.5.7 Leak Sensor Test" P. 7-15 LEAK SENSOR TEST Inject and Enter 5-30 CTO-20A/20AC...
Page 79: Calibration Support Group
Password should be registered by the system manager. Input password one digit at a time using the numeric keypad, and press ∗ Ask your Shimadzu service representative for details about passwords. If the password input is correct, [TEMP1 CALIB] (the Next function next function) will be displayed.
Page 80: Leak Thr
When using the SCL-20A, control is possible with any setting. Some functions are restricted in the compatibility modes. Use the numeric keypad to enter the value and press Mode value CTO-20A mode CTO-10Avp compatibility mode (When using the SCL-10Avp) CTO-10A compatibility mode (When using the SCL-10A) [INITIALIZE PARAM] Initializes the parameters (except those of the VP function and [LINK ADRS]), and deletes the time programs.
Page 81: Change Password
To confirm, input the same password again. Input Again When the new password is registered, Valid [PASSWORD CHANGED] appears. CHANGE PASSWORD PASSWORD CHANGED Not valid If not, [PASSWORD WRONG] appears. CHANGE PASSWORD PASSWORD WRONG Press to return to the title screen. CTO-20A/20AC 5-33...
Page 82: Creating Time Programs
Number of steps used/remaining display The display on the right appears. Number of steps already used Used Left Number of steps remaining In this example, 10 steps have been set, and 310 more may be programmed. 5-34 CTO-20A/20AC...
Page 83: Time Program Flow
Press after setting the value using the numeric keypad. Set the next operation in the program. ∗ 1 Press to return to the previous step. ∗ The valid time setting range is 0.1-999.9 minutes. CTO-20A/20AC 5-35...
Page 84: Setting Functions
After setting the time for the step, press [Func] appears. Press until [TMPL] is Time Func Value displayed.Then press to set [TMPL]. 20.0 TMPL Use the numeric keypad to set the desired temperature (4- 85°C). Press to proceed to the next step. 5-36 CTO-20A/20AC...
Page 85: [On (Oven On) / Off (Oven Off)]
[ON/OFF] is displayed. Then press to set [ON/OFF]. Time Func 10.1 ON NOTE Time Func 45.0 OFF [ON] and [OFF] have no values associated with them. Therefore, the display proceeds directly to the next step when is pressed. CTO-20A/20AC 5-37...
Page 86: Adding Steps For Time Program
T i m e F u n c V a l u e 1 2 . 0 E v n t NOTE It is not necessary to enter new steps in chronological order. The unit automatically sets them in the correct order. 5-38 CTO-20A/20AC...
Page 87: Deleting Steps
If there is no [STOP] command, the program will be implemented for the maximum time of 999. After setting the time, press [Func] appears. Press until [STOP] is Time Func Value displayed. Then press to set [STOP]. 50.0 STOP Press . initial screen is displayed, and a time program ends. CTO-20A/20AC 5-39...
Page 88: [Evnt (Event Output On/Off)]
[Func] appears. Press until [RV-L] or [RV- Time Func Value R] is displayed. Then press to set [RV-L] 25.0 RV-L or [RV-R]. Configure settings 0, 1 or 1-6 via the numeric keypad. Press to proceed to the next step. 5-40 CTO-20A/20AC...
Page 89: Loop
• Value of [LOOP] command can be set to 255. When [0] is set, time program will be repeated 256 times. • Steps which follow a [LOOP] command are ignored. The program finishes when execution of the [LOOP] command has been completed. Settings return the values before program execution. CTO-20A/20AC 5-41...
Page 90: Creating A Typical Time Program
The number of steps already created and remaining steps appears. Used Left Press Time setting display The setting screen appears. Time ™ Using the numeric keypad, set 20 (minutes) for Time setting for the 1st step the time. Then press [Func] appears. Time 20.0 5-42 CTO-20A/20AC...
Page 91 Temperature setting for the 2nd step [Value] (temperature). Then press Time setting for 2nd step is complete and Time Func Value proceeds to the next step. 150.0 TEMP [Time] appears on the screen. The 3rd step Time 150.0 CTO-20A/20AC 5-43...
Page 92 [STOP] is Function setting for the 4th step displayed. Then press to set [STOP] as the command.The time program is now complete. Time Func 201.0 STOP Press to return to the initial screen. Initial screen SET ACTUAL T.MAX XX.X 5-44 CTO-20A/20AC...
Page 93: Creating A Typical Time Program2 -Linear Temperature Regulation
Used Left Press Time setting display The setting screen appears. Time ™ Using the numeric keypad, set 60 (minutes) for Time setting for the 1st step the time. Then press . [Func] displays. Time 60.0 CTO-20A/20AC 5-45...
Page 94 120.0 TMPL Using the numeric keypad, set 50 (minutes) for the Temperature setting for the 2nd step time. Then press Time setting for 2nd step is complete and Time Func Value proceeds to the next step. 120.0 TMPL 5-46 CTO-20A/20AC...
Page 95: Starting A Time Program
There are two ways to stop the time program: • Press to stop the running program forcefully. The [prog.run] indicator goes out. • Insert [STOP] command of the program. P.5-39 When the program stops, the [prog.run] indicator goes out. CTO-20A/20AC 5-47...
Page 96: Control By Cbm-20A Or Cbm-20Alite System Controller
The operating temperature, maximum temperature, automatic column switching valves (L, R), column oven ON/OFF, and the time program are set from the system controller. The Column Management Device (CMD) is also supported. "9.1.12 Column Management Device (CMD)" P. 9-33 For details, see the CBM-20A/20Alite Instruction Manual. 5-48 CTO-20A/20AC...
Page 97: Control By Scl-10Avp Or Scl-10A System Controller
CTO-10A/Cvp or the CTO-10A/C and so the following restrictions apply: 1) The maximum possible settings for the operating temperature and maximum temperature are 80°C and 85°C respectively. 2) The Column Management Device (CMD) cannot be used. "9.1.12 Column Management Device (CMD)" P. 9-33 CTO-20A/20AC 5-49...
Page 98: Connection To External Input/Output Terminals
[OVEN OFF] input terminal. [OVEN OFF] signal stops (input) temperature regulation. [EVENT1] [Event1/2] output terminals. Internal relay contacts (input) which are turned ON/OFF according to a time Contact rating: 30VDC/0.1A [EVENT2] program or the [EVENT] parameter value. (input) 5-50 CTO-20A/20AC...
Page 99: Connection Of Event Cable
• Cable with single wire: φ 0.4 to φ 1.2 (AWG26 to 16) (AWG22 to 16), diameter of single wire thicker than φ 0.18. • Cable with stranded wire: 0.3mm to 1.25mm The cable with stranded wire is suitable to prevent disconnection. CTO-20A/20AC 5-51...
Page 100 5. Application Operation NOTE If [EVENT1] or [EVENT2] signal is used, set [EVENT] and [EXT-S] parameters. EVENT "[EVNT (EVENT Output ON/OFF)]" P. 5-40 Input 0,1,2,12 "[EXT-S]" P. 5-19 EXT-S Input 0 - 3 5-52 CTO-20A/20AC...
Page 101: Chapter 6 Troubleshooting
Troubleshooting Contents Troubleshooting and Corrective Action ............6-2 Error Messages....................6-3...
Page 102: Troubleshooting And Corrective Action
For more detailed procedures, refer to the indicated page. If the problem cannot be resolved even after taking the indicated measures, or if there are problems not included in the following tables, contact your Shimadzu representative. Symptom...
Page 103: Error Messages
Error message Type Cause and Action Fatal Cause: ROM error (electronic failure) ROM FAILURE Action: Turn power OFF and contact your Shimadzu (ROM error) representative. Fatal Cause: RAM error (electronic failure) RAM FAILURE Action: Turn power OFF and contact your Shimadzu (RAM error) representative.
Page 104 Fatal Cause: Thermosensor readings are abnormally high. ERR SENSOR H There may be a short circuit in the sensor. (Thermosensor error) Action: Turn power OFF and contact your Shimadzu representative. Fatal Cause: Thermosensor readings are abnormally low. ERR SENSOR L The sensor may be disconnected.
Page 105 Fatal Cause: Rotation error in an automatic column-switching ERR NO RV-L HOME valve. (Left valve home position error) Action: Turn power OFF and contact your Shimadzu representative. ERR NO RV-R HOME (Right valve home position error) Warning An error message is displayed, and an alarm sounds, WARNING: approximately 10 seconds after occurrence of the error.
Page 106 6. Troubleshooting This page is intentionally left blank. CTO-20A/20AC...
Page 107: Chapter 7 Hardware Validation
Hardware Validation This chapter provides instruction on hardware validation, which verifies the performance of individual components and the instrument as a whole. Contents Overview of Hardware Validation..............7-2 Implementation of Hardware Validation ............7-3 Validation Precautions ..................7-4 Equipment Required for Validation ..............7-5 Validation : Column Oven ................
Page 108: Overview Of Hardware Validation
The operational protocol and criteria for this component and the HPLC system are described in this chapter to assist the user in conducting validation. Refer to each the instruction manual for each component for operational protocol of that specific component. CTO-20A/20AC...
Page 109: Implementation Of Hardware Validation
After any maintenace, component performance must be re-validated. The type of validation depends on the actual work done. If the maintenance inspection cannot be performed solely by the specific component validation, system validation is required. NOTE Maintenance information and results of hardware validation must be recorded and kept for future reference. CTO-20A/20AC...
Page 110: Validation Precautions
• Provide adequate installation surface and space The weight of CTO-20A is approximately 20kg, and CTO-20AC approximately 23kg. During installation, consider the entire weight combined with other LC components.
Page 111: Equipment Required For Validation
For inspection of the flow rate accuracy for the solvent delivery module. Measuring flask Obtain a 5mL-measuring flask. For inspection of the injection volume accuracy for the autosampler. Electronic balance The balance must be calibrated and able to perform measurement with a 0.001g precision at the time of inspection. CTO-20A/20AC...
Page 112: Standard Reagents For Validation
For inspection of the wavelength accuracy for the spectrofluorometric PTFE block assembly 228-34319-91 detector. Column Particle size: 5µm Shim-pack VP-ODS Column Dimension: I.D. 4.6mm × length 150mm 228-34937-91 (An equivalent ODS column may also be used.) LUNA C18(2) For the system validation. 00F-4252-E0 CTO-20A/20AC...
Page 113: Validation : Column Oven
Turn the power switch ON. Press three times to display [VALIDATION]. Press three times to display [MEMORY CHECK]. MEMORY CHECK ROM OK / RAM OK Press "[MEMORY CHECK]" P. 5-29 CHECK CRITERIA: [ROM OK / RAM OK] is displayed on the screen. CTO-20A/20AC...
Page 114: Firmware Version Check
Press two times to display the version number. S/W ID: V*.** "[S/W ID]" P. 5-25 CTO-20A(c) CHECK CRITERIA: Version number appears. The number is same as the administrated one. 7.5.4 Display, LED Test Objective To check the operation of display and LEDs.
Page 115: Setting Temperature Accuracy And Regulation Accuracy (40/50°C) Check
Attach the thermosensor (platinum resistor) to the Column bracket column bracket inside the oven, in front of the oven's platinum sensor. Use the thermal insulation material inside the tubing hole. Platinum Latch Thermal sensor insulation disc Column clip Fig. 7.1 CTO-20A/20AC...
Page 116: Check Procedure
50.0 Press repeatedly until the initial screen displayed. When the [ready] indicator illuminates, wait 10 Lights minutes. NOTE When running the [TEMPERATURE TEST], set [WAIT TIME] to 5 minutes and [READY RANGE] to 0.1. How to set P.5-30 7-10 CTO-20A/20AC...
Page 117 Observe the result displayed. Setting CHECK CRITERIA: REF TEMP 50.1 Temperature accuracy at 40/50°C is ± 2.0°C. [ACCURACY GOOD] appears. In case of NG, "7.6 Temperature accuracy calibration" P. 7-17 O.K. ACCURACY GOOD N.G. ACCURACY NG Calibrate CTO CTO-20A/20AC 7-11...
Page 118: Setting Temperature Accuracy And Regulation Accuracy (Room Temperature 5°C) Check
Attach the thermosensor (platinum resistor) to the Column column bracket inside the oven, in front of the bracket oven's platinum sensor. Use the thermal insulation material inside the tubing hole. Platinum Latch Thermal sensor insulation disc Column clip Fig. 7.3 7-12 CTO-20A/20AC...
Page 119: Check Procedure
Press repeatedly until the initial screen 23.0 displayed. When the [ready] indicator illuminates, wait 10 minutes. Lights NOTE When running the [TEMPERATURE TEST], set [WAIT TIME] to 5 minutes and [READY RANGE] to 0.1. How to set P.5-30 CTO-20A/20AC 7-13...
Page 120 Setting CHECK CRITERIA: REF TEMP 50.1 Temperature accuracy at room temperature - 5°C is ± 2.0°C. [ACCURACY GOOD] appears. In case of NG, "7.6 Temperature accuracy calibration" P. 7-17 O.K. ACCURACY GOOD N.G. ACCURACY NG Calibrate CTO 7-14 CTO-20A/20AC...
Page 121: Objective
Use the numeric keypad to set the temperature to 50°C or 40°C and press SET TEMP Input 4 - 85 Press Temperature regulation starts. Press repeatedly until the initial screen SET ACTUAL T.MAX appears. 50.0 When the [ready] indicator illuminates, wait for 10 minutes. Lights CTO-20A/20AC 7-15...
Page 122 20 seconds. LEAK SENSOR TEST SENSOR GOOD CHECK CRITERIA: [SENSOR GOOD] appears. N.G. In case of NG, LEAK SENSOR TEST "8.2 Adjusting the Leak Sensor Sensitivity" P. 8-3 SENSOR NO GOOD Injection (100µL) Methanol 100µL Syringe Fig. 7.5 7-16 CTO-20A/20AC...
Page 123: Temperature Accuracy Calibration
The first calibration temperature must be no greater than 60°C and the second calibration temperature must be at least 20°C higher than the first calibration temperature. Before delivery, the CTO-20A and the CTO- 20AC are calibrated at 40°C and 60°C and at 25°C and 60°C respectively.
Page 124 REF. TEMP2 CALIB 59.8 Enter the temperature read from the thermosensor as the correction parameter in step This completes the procedure for temperature accuracy calibration. This setting result remains in memory even if the power is turned off. 7-18 CTO-20A/20AC...
Page 125: System Validation
Weigh 20mg of anhydrous caffeine, transfer to a 100mL volumetric flask and dilute to volume with water. Transfer 1mL of the solution to a 10mL volumetric flask, and dilute to volume with water. Water HPLC grade, or equivalent 2-propanol HPLC grade, or equivalent CTO-20A/20AC 7-19...
Page 126: Checking And Preparing The Lc System
(methanol, acetonitrile, etc.) > Clean the flow lines with water. Pass water through the flow lines for 10 minutes, at a rate of 2mL/min. 7-20 CTO-20A/20AC...
Page 127: Validation Procedure
"Parameter Settings for Isocratic System Validation" P. 7-22 See the autosampler's instruction manual for setting procedures. Set the data processor parameters. "Parameter Settings for Isocratic System Validation" P. 7-22 See the data processor's instruction manual for setting procedures. CTO-20A/20AC 7-21...
Page 128: Parameter Settings For Isocratic System Validation
: 1000 ATTEN : 10 (1,024mAUFS) SLOPE : 1000 MIN.AREA : 100000 STOP.TM CHECK CRITERIA The RSD (C.V.)'s obtained must satisfy the following criteria: Retention time RSD must not exceed 0.5%. Peak area RSD must not exceed 1.0%. 7-22 CTO-20A/20AC...
Page 129: Validation Of Gradient Lc System
(b) the column outlet and the detector inlet, has an I.D. of less than 0.3mm, and is shorter than 300mm. Keep the liquid volume that is not in the column as low as possible. Fig. 7.9 CTO-20A/20AC 7-23...
Page 130: Validation Procedure
Set the pumping flow rate to 1mL/min, and set the Pump's display screen concentration of mobile phase B parameter to 40%. 1.000mL MAX 20.0 See the pump's instruction manual for setting 0.0MPa MIN procedures. A: 60.0 B:™ 40.0 0.0 D: 7-24 CTO-20A/20AC...
Page 131 : Number of analyses (coefficient of variation [C.V.]) for: retention time X1••Xn : Retention time (or areas) of each peak and peak area ("Fig. 7.11"). : Average : Standard deviation : Relative standard deviation C.V. : Coefficient of variation Fig. 7.11 CTO-20A/20AC 7-25...
Page 132: Parameter Settings For Gradient System Validation
T.DBL : 1000 ATTEN : 10 (1,024mAUFS) SLOPE : 1000 MIN.AREA 100000 STOP.TM CHECK CRITERIA The RSD (C.V.)'s obtained must satisfy the following criteria: Retention time RSD must not exceed 0.5%. Peak area RSD must not exceed 1.0%. 7-26 CTO-20A/20AC...
Page 133: If Validation Fails
For troubleshooting procedures for individual system components, see the applicable instruction manuals. • If a cause cannot be determined, contact your Shimadzu representative: If you are unable to determine the cause of the failure, or if you are unclear about troubleshooting or corrective action procedures, contact your Shimadzu representative.
Page 134 7. Hardware Validation This page is intentionally left blank. 7-28 CTO-20A/20AC...
Page 135: Chapter 8 Maintenance
Maintenance Periodic Inspection and Maintenance .............. 8-2 Adjusting the Leak Sensor Sensitivity .............. 8-3 Exterior Cleaning....................8-7...
Page 136: Periodic Inspection And Maintenance
Periodic Inspection and Maintenance It is necessary to perform periodic inspections of this instrument to ensure its safe use. It is possible to have these periodic inspections performed by Shimadzu service representatives on a contractual basis. For information regarding the maintenance inspection contract, contact your Shimadzu representative.
Page 137: Adjusting The Leak Sensor Sensitivity
Starting from the initial screen, press until [CALIBRATION] is displayed. CALIBRATION Press func or VP Press [Input PASSWORD] will be displayed. Input PASSWORD Input the correct password. [TEMP1 CALIB] (the next function) will be displayed. TEMP1 CALIB 50.0 Input 4.0 - 60.0 CTO-20A/20AC...
Page 138 (Display Only) is displayed. Draw 50µL of methanol into the provided syringe. Injection of methanol (50µL) Inject the 50µL of methanol into the oven through the drain located on the right bottom of the oven. Methanol 50µL syringe Fig. 8.1 CTO-20A/20AC...
Page 139 In the example screen on the right, [13800] has been set. Press to save the value permanently. Run the [LEAK SENSOR TEST] (procedure on "7.5.7 Leak Sensor Test" P. 7-15). If [SENSOR GOOD] appears as the test result, adjustment is complete. CTO-20A/20AC...
Page 140: Corrective Action When Leakage Occurs
Close the right door, and press to start temperature regulation. ERR LEAK DETECT If the [ERR LEAK DETECT] message does not CE to Clear reappear, operation can be resumed. If reappears, clear the error message by pressing repeat steps 1-3 above. CTO-20A/20AC...
Page 141: Exterior Cleaning
Use a dry cloth to remove all moisture from the exterior surface of the instrument. NOTE Do not allow spilled water to remain on the instrument surface, and do not use alcohol or thinner-type solvents to clean the surfaces. These can cause rusting and discoloration. CTO-20A/20AC...
Page 142 8. Maintenance This page is intentionally left blank. CTO-20A/20AC...
Page 143: Technical Information
Technical Information Contents Installation ......................9-2 Specifications ....................9-35 Maintenance Parts ..................9-36 Introduction to HPLC System................. 9-37 Mobile Phase Characteristics................. 9-40...
Page 144: Installation
• air currents from heating or air conditioning equipment are not directed on the instrument. • sunlight does not shine directly on the instrument. • there is no vibration. • humidity stays within 20 - 85%. • place without condensation CTO-20A/20AC...
Page 145: Required Installation Space
Required Installation Space CAUTION • The weight of CTO-20A is approximately 20kg, and CTO-20AC approximately 23kg. During installation, consider the entire weight combined with other LC components. The lab table on which this instrument is installed should be strong enough to support the total weight of the LC system.
Page 146: Installation
Stacking bracket available. Fasten the instrument firmly in place by attaching stacking brackets to both the right and left sides. For more details, contact your Shimadzu representative. Safety bracket An example of stacking bracket placement is shown in "Fig. 9.3".
Page 147: Power Supply Connection
9.1 Installation 9.1.3 Power Supply Connection The following table shows the electrical voltage, power consumption, and frequency. Part No. Power supply voltage Consumption Frequency (indicated on the instrument) CTO-20A CTO-20AC ∼ AC100V (100V 228-45009-31 228-45010-31 500VA 50/60Hz ∼ AC120V (120V...
Page 148: Connection To Power Outlet
Connect to Power outlet Fig. 9.6 Grounding WARNING The three-line type power cable provided as an accessory includes the grounding wire. Be sure to ground through this cable in order to prevent electrical shock and to ensure stable operation of the instrument. CTO-20A/20AC...
Page 149: Prior To Plumbing
Cut up the tubing so that the cut surface is at a right angle. Fig. 9.7 Holding the tubing at equal distances from the cutting up line, bend it up and down and from side to side to cut off. File the cut surface to make smooth and straight. Fig. 9.8 CTO-20A/20AC...
Page 150: Connecting Tubings
Ferrule Install stainless steel male nuts and ferrules on SUS tubing, and resin nuts and ferrules on resin tubing. If resin male nuts are mounted on SUS tubing, the nuts will be damaged and leakage may occur. Fig. 9.10 CTO-20A/20AC...
Page 151 • For a resin male nut: Tighten and loosen the nut by hand. Coupling Fig. 9.12 Loosen and move the male nut slightly to verify that the ferrule is secured on the tubing. Moves Secured Fig. 9.13 CTO-20A/20AC...
Page 152: Protective Plugs
Keep the plugs, and replace them when the instrument will be left not in use for a long time. NOTE • For stop plugs: Use the wrench provided to unscrew and screw in the plugs. • For resin plugs: Remove and replace the plugs by hand. 9-10 CTO-20A/20AC...
Page 153 P.9-19 P.9-20 oven Connecting Leakage Drain Tubing Pump P.9-13 Reservoir bottle Manual injector installation P.9-15 Manual injector plumbing Waste container P.9-17 preparation P.9-12 For plumbing between the pump and reservoir bottle, see the pump instruction manual. Fig. 9.14 CTO-20A/20AC 9-11...
Page 154: Plumbing
Tubing and outer caps and the thermal insulation disc. Thermal Insulation disc Plumbing Replace the inner and outer caps and the thermal inlets/outlets Plumbing insulation disc into the plumbing inlet/outlet. inlet/outlet cap(outer) Plumbing inlet/ outlet cap(inner) Fig. 9.16 9-12 CTO-20A/20AC...
Page 155: Connecting Leakage Drain Tubing
ERR LEAK DETECT the bottle initially, the leak sensor could erroneously CE to Clear signal a leak. Post-plumbing check Pour water into the drain pan, and verify that the water drains to the waste bottle, without any backflow. CTO-20A/20AC 9-13...
Page 156: Column Bracket Installation
• The bracket can be moved to position required to accommodate various devices and accessories. Fig. 9.18 Installing Latches Press on the latch until it clicks. Latch The latch is now locked in place. Press Locked Fig. 9.19 9-14 CTO-20A/20AC...
Page 157: Manual Injector Installation
Type 9725i controller or Chromatopac. The manual injector can be installed in either of two manual injector positions in the left door. Manual injector position Plumbing position Manual injector installation area Vial holder position Left door Fig. 9.20 CTO-20A/20AC 9-15...
Page 158 (For details on handling the manual injector, see the instruction manual for the manual injector.) NOTE If the injector has a position sensing switch, route the switch signal cable through the tubing outlet and connect Manual it to the switch. injector Fig. 9.23 9-16 CTO-20A/20AC...
Page 159: Flow Line Plumbing
Install a male nut (with long bushing) and ferrule to one end of each of the two waste liquid tubing Waste liquid sections. Then attach the tubing and ferrules into tubing ports 5 and 6 of the manual injector. Tighten the nuts. Waste liquid tubing Fig. 9.25 CTO-20A/20AC 9-17...
Page 160 Waste liquid perpendicular to the left door. If the tubing curves tubing outward, it could lodge against the side of the unit and Lodging prevent closing of the left door. against Manual side injector Perpendicular Left door Fig. 9.28 9-18 CTO-20A/20AC...
Page 161: Plumbing Between Pump Unit And Manual Injector
2 of the manual injector, and tighten the male nuts. NOTE The SUS tubing should have some extra length. Otherwise, it will not bend easily, and may prevent the front door from closing. Fig. 9.29 CTO-20A/20AC 9-19...
Page 162: Plumbing Between Manual Injector And Column
From pump outlet Column NOTE inlet If the SUS tubing has no extra slack, unscrew and remove the male nut from the column inlet before opening the left door. SUS tubing Fig. 9.31 9-20 CTO-20A/20AC...
Page 163: Plumbing Between Column And Detector
Arrows indicate the direction of flow. Male nut PEEK Cell outlet Detector Blue cover Male Nut PEEK Cell inlet Male nut PEEK Stop plug Column outlet Waste container Unscrew and remove stop plug from Autosampler/Manual Injector Fig. 9.32 CTO-20A/20AC 9-21...
Page 164: Installation And Plumbing Of Mixer (Option)
Attach caps to ports which are not used, to prevent dirt or dust entering through them. 0.5mL Plumbing 1.7mL Plumbing 2.6mL Plumbing Attach caps Attach cap to Autosampler/ Manual Injector to Autosampler/ Manual Injector to Autosampler/ Manual Injector Attach cap Fig. 9.34 9-22 CTO-20A/20AC...
Page 165: Mounting The Mixer On The Instrument
(In this position, the mixer is installed upsidedown, as shown in the figure on the right.) NOTE If a manual injector has been installed on the left door, install the mixer on the right. This facilitates both maintenance and door usage. Fig. 9.37 CTO-20A/20AC 9-23...
Page 166: Mixer Plumbing (In Low-Pressure Gradient System)
SUS tubing. Inlet B Attach the ends of one tubing section and ferrule into the pump outlet and premixer inlet B, and screw in the male nuts. Fig. 9.40 9-24 CTO-20A/20AC...
Page 167: Mixer Plumbing (In High-Pressure Gradient System)
Connect the pump outlet to the premixer inlets A Fig. 9.42 and B with the SUS tubing. NOTE For 3 mobile phases, also unscrew and remove the stop plug from inlet C, and connect inlet C to the pump 3 outlet with more SUS tubing. CTO-20A/20AC 9-25...
Page 168 2 of the manual injector, and screw in the male nuts. NOTE If an autosampler is used, see its instruction manual for procedures. to Port 2 from Mixer outlet Fig. 9.44 9-26 CTO-20A/20AC...
Page 169: Wiring
[REC] connector For connection of the (column oven) temperature sensor's output signal (100°C/mV). NOTE [REC] connector is used for making adjustments and is not normally required. Power supply connector For connection of the power supply cable. Fig. 9.45 CTO-20A/20AC 9-27...
Page 170: Connecting The Optical Cable
• Do not bend the cable where it joins the plug. Failure to follow these above precautions could result in damage to the plug or a broken wire in the cable. Fig. 9.48 9-28 CTO-20A/20AC...
Page 171: Connection To System Controller
Check the [LINK ADRS] and [LOCAL] parameters. "5.2.5 System Settings Group" P. 5-17 LINK ADRS • [LINK ADRS]: Enter the system controller Input 1 - 12 REMOTE channel number. • [LOCAL]: Enter [0] (Remote mode). LOCAL 0:Remote 1:Local CTO-20A/20AC 9-29...
Page 172: Installation Of Ambient Temperature Sensor Accessory
Use the clamp to secure the ambient temperature sensor cable at a location near the instrument with minimal temperature fluctuation. NOTE If the tip of the ambient temperature sensor touches the unit's rear cover, the sensor cannot correctly sense the temperature. 9-30 CTO-20A/20AC...
Page 173: Installation Of Auto Column Switching Valve
WARNING Installation of these valves is dangerous (due to electrical hazards). Do not install the valves yourself. Have them installed by your Shimadzu service representative. The installation positions and flow paths of the valves are shown below for your reference.
Page 174: Connection To Chromatopac
Signal cable (accessory for Black (-) Chromatopac) Signal cable White (+) To Chromatopac Fig. 9.53 Connect the signal cable (provided) to the column oven [REC] connector. Then make the connections to the Chromatopac as shown in the figure above. 9-32 CTO-20A/20AC...
Page 175: Column Management Device (Cmd)
Attach the CMD to the column. Column Fig. 9.54 Set the column with the CMD attached. Fig. 9.55 Insert the CMD cable (Part No. 228-39991-01) in the CMD. CMD cable Fig. 9.56 CTO-20A/20AC 9-33...
Page 176 CMD cable from the oven during analysis. Doing so may result in the loss of data. • CMD Do not detach the CMD from a column when removing the column from the CTO-20A/20AC after finishing analysis. Store the column with the CMD attached. When the column's service life expires, however, the CMD can be detached and used with other columns after performing initialization.
Page 177: Specifications
W 260mm × D 420mm × H 415mm Weight 20kg 23kg AC 100V 500VA 228-45009-31 228-45010-31 Power AC 120V 600VA 228-45009-32 228-45010-32 supply AC 220-240V 600VA 228-45009-38 228-45010-38 ∗ 1 Room temperature means the ambient temperature at the CTO-20A/AC. CTO-20A/20AC 9-35...
Page 178: Maintenance Parts
9. Technical Information Maintenance Parts 9.3.1 Replacement Parts Part Part No. Remark PCB ASSY, CTO-20A (with ROM) 228-41880-91 For 20A, with ROM PCB ASSY, CTO-20AC (with ROM) 228-41880-92 For 20AC, with ROM PCB ASSY, CTO-X 228-41860-92 without ROM CTO PW switch ASSY...
Page 179: Introduction To Hplc System
9.4 Introduction to HPLC System Introduction to HPLC System The Prominence LC (LC-20A) series components are for use with Shimadzu high performance liquid chromatography (HPLC) systems, which are designed to provide high accuracy and high sensitivity analyses. Example system configurations are provided below, along with descriptions of the operations of the various components.
Page 180: Example Of Autosampler System (1)
In the column, the components are separated by means of the mutual interactions of the mobile phase and the column packing (stationary phase). The detector detects the components separated in the column, and sends the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. 9-38 CTO-20A/20AC...
Page 181 The detector detects the components eluted from the column, and send the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. The CBM-20A system controller can control a maximum of 8 LC components (12 LC components as an option) including a maximum of 4 pump units. CTO-20A/20AC 9-39...
Page 182 26 Ethyl iodide 1.510 0.57 27 n-Octanol 1.427 10.3 28 Fluorobenzene 1.46 0.55 29 Benzylether 1.538 30 Methylene chloride(**) 1.421 0.41 0.42 0.17 31 Anisole 1.514 32 i-Pentanol 1.405 0.61 14.7 33 1,2-Dichloroethane 1.442 0.78 0.44 0.16 10.4 9-40 CTO-20A/20AC...
Page 183 0.80 36.7 64 Dimethylsulfoxide 1.477 2.00 0.62 miscible 65 N-methyl-2-pyrolidone 1.468 1.67 Hexamethyl phosphoric 1.457 0.65 acid triamide 67 Methanol(*) 1.326 0.54 miscible 32.7 68 Nitromethane 1.380 0.61 69 m-Cresol 1.540 11.8 10.0 70 N-methylformamide 1.447 1.65 miscible CTO-20A/20AC 9-41...
Page 184 (7) Water solubility (%W) at 20°C of solvent used in liquid-to -solid adsorption. (8) Value at 20°C. (9) Function consisting of P' (proportional to solvent strength) plus the dielectric constant, in ion chromatography. Source: A.Krstulovic and P.R.Brown, Reversed-Phase High-Performance Liquid Chromatography, Wiley Interscience, 1982. 9-42 CTO-20A/20AC...
Page 185 DATE ............5-28 LEAK SENSOR ..........5-30 Deleting Steps ..........5-39 Leak Sensor Test .......... 7-15 Display Panel ...........2-7 LEAK THR ............ 5-32 Linear Temperature Regulation ....5-45 LINK ADRS ........... 5-17 List of Auxiliary Functions ......5-10 CTO-20A/20AC Index...
Page 186 Plumbing between Pump Unit and Manual VP Functions ..........5-22 Injector ............9-19 VP Function Screens ........5-6 Power Supply Connection ....... 9-5 Prior to Plumbing ..........9-7 PROG TIME ..........5-15 WAIT TIME ............ 5-20 Wiring ............9-27 CTO-20A/20AC Index...

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