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Axion MAESTRO EDGE Hardware Manual

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MAESTRO EDGE™
Hardware Guide

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Summary of Contents for Axion MAESTRO EDGE

  • Page 1 MAESTRO EDGE™ Hardware Guide...

  • Page 2: Declaration Of Conformity

    Declaration of Conformity This instrument meets the requirements for ANSI/UL 61010-1 and CAN/CSA-C22.2 No. 61010-1. The following standards were applied: IEC/EN 61326 (EMC) and IEC/EN 61010-1 (Safety). The user is responsible for providing a compatible electromagnetic environment in order for the device to perform as intended. Do not use this device in close proximity to sources of strong electromagnetic radiation, (e.g.
  • Page 3 Thank you Thank you for purchasing the Maestro Edge, Axion’s powerful integrated system for neural and cardiac electrophysiology, and impedance-based cell analysis. Please contact us with any questions regarding system operation. Intended Use: The Maestro is intended for benchtop research experiments only; it is not intended for use in diagnostic procedures.
  • Page 4 Included Equipment & Symbols Used 1 Maestro Edge Warning Symbol in Manual 1 Smart Plate Power On/Off 1 AxIS User Guide 1 Power Cable Ethernet 1 Ethernet Cable 2 Air Filters 1 Door Cleaning Cloth 10 feet of CO2 polyurethane tubing (6mm outer diameter)
  • Page 5 Plate Button Light Functions White then Blue Pulsing Maestro is starting up. Blue Maestro is open/closed without plate. Green Pulsing Maestro is streaming with plate. Green Maestro is closed with plate. Maestro is streaming but could not register a plate Yellow Pulsing barcode.
  • Page 6 Recommended Set-up Environment The Maestro contains very sensitive electronic equipment that may pick up external environmental interference (e.g. table bumps, cell phone signals, power outlet emissions, etc.). The Maestro has many features that assist in mitigating this interference, but proper instrument placement can help.
  • Page 7 System Set-up Instructions The Maestro is designed for quick and easy set-up. 1) Power - Plug the Power Cable into the back of the Maestro and the plug into a wall outlet. WARNING: To avoid the RISK of electric shock, this equipment must only be connected to a SUPPLY MAINS with protective earth.
  • Page 8 Power Button - Turns the system on/off. The system will take a minute to boot up or shut down. Plate Button - Docks and undocks Axion plates. The button color indicates AxIS mode (ready, recording, stimulating, error). Door - Opens automatically when the plate is ejected. To open or close the door while a plate is engaged, gently push handle.
  • Page 9 Door Power Button Plate Button...
  • Page 10 Working with the Plate Chamber Plate A1 Notch - Proper orientation of plate with A1 notch in top left. Pogo Pin Connector - Connects plate to Maestro electronics. Heater Plate - Maintains temperature throughout experiment. Refer to the AxIS Navigator User Guide for details.
  • Page 11 Pogo Pin Plate A1 Notch Connector Heater Plate...
  • Page 12 Working with the Back Panel Mains Power Switch - Use only when unplugging the system. Must be in the "I" position for the system to power up. CO2 Input - Connect 100% CO2 (15 psi; 6 mm OD tubing). Air Filter - Attach supplied air filter to luer lock fitting.
  • Page 13 CO2 Input Air Filter Ethernet Filter Input Ventilation Lumos Power Plug In/Out Mains Power Switch...
  • Page 14 5) For MEA experiments, push the play button in AxIS Navigator to display cellular activity. For detailed instructions on using the Maestro Edge for MEA experiments, please review the AxIS Navigator user guide, which can be found in the AxIS Navigator Help menu.
  • Page 15 The Pogo Pins and external air filter are the only user serviceable parts. Please contact Axion Support at support@axionbio.com or 404-477-2557 for any other maintenance. Storage When the Maestro is not in use, Axion recommends leaving the Smart Plate engaged with the door closed. Instrument Disposal...

  • Page 16: Air Filter Replacement

    Air Filter Replacement The Maestro Edge’s CO2 controller requires clean air to maintain a stable environment for your cells. To replace the air filter, twist luer fitting to remove the tube from the air filter, then twist filter counter-clockwise to remove it from the back of the Maestro. Twist the new filter in place on the Maestro until snug.
  • Page 17 • Ensures critical data accuracy • Trained and qualified factory engineers • Keeps systems at peak performance • Minimum instrument downtime • Maximizes productivity • Peace of mind • Reduces lab expenses For pricing and maintenance plan tiers, please contact: Axion Service Team service@axionbio.com...

  • Page 18: Technical Specifications

    Technical Specifications Power Supply Specifications: System Dimensions: 100-240V AC Voltage Size 280 x 413 x 225 mm (W x D x H) 8-4A AC Current Weight 29.2 lbs 60-50Hz Frequency Operating Environment: Environmental Controls: Recommended CO2 Temperature 20-32oC 10-15 psi (0.7 - 1 bar/0.06 - 0.1 Pressure MPa) Max Relative...
  • Page 19 Technical Specifications Neural and Cardiac Recording: Sampling Rate 12.5kHz (384 channels) System Bandwidth 0.01 Hz - 5 kHz System Gain 100 V/V - 1000V/V MEA Viability Dynamic Range 5 kΩ - 100 kΩ Neural and Cardiac Stimulation: Stimulation Voltage (max) +/-1.2 V Stimulation Current (max) +/- 250 µA...
  • Page 20 Technical Specifications Impedance: Impedance Measurement Signal +/- 5mV at 1, 10, 41.5 kHz Impedance Measurement Repeatability +/- (0.5% + 1 Ω) Impedance Measurement Uniformity +/- (1% + 1.5 Ω) Impedance Sampling Rate 1 Sample/Minute (Automatic, All Wells) Impedance Dynamic Range 50 Ω...

  • Page 21: Further Information

    Further Information Systems Discover the innovation behind the Maestro cell analysis platforms. www.axionbiosystems.com/products Software Customize your Maestro to meet your assay needs with Maestro software modules. www.axionbiosystems.com/software Applications Discover the functionality of your cells with MEA electrical network analysis and impedance-based cell analysis.
  • Page 22 Distributed by STEMCELL Technologies Inc. Have questions about the Maestro Pro? We are here to help. Contact STEMCELL Technologies at techsupport@stemcell.com www.stemcell.com/info-maestro-pro...
  • Page 23 Notes...

This manual is also suitable for:

Maestro z

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