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Summary of Contents for Ecom ECP201L
- Page 1 ECP201L / ECP201LG User Guide ECP201L / ECP201LG HPLC P REPARATIVE ECOM spol. s r.o., Trebonicka 239, CZ252 19 Chrastany u Prahy, Czech Republic tel: +420 221 511 310, info@ecomsro.cz, www.ecomsro.com VAT: CZ411 92 192...
- Page 2 ECP201L / ECP201LG Document properties File: Manual-ECP201L_ECP201LG-en-04.doc Number of pages: File size: 11391 kB 8/20/2024 11:44:00 AM Kateřina Kněžourková Last save: Ver. Date Author Approved by Description Kateřina Kněžourková Jakub Hodaň 03.10.2023 The new declaration of conformity. Kateřina Kněžourková Jakub Hodaň...
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Page 3: Table Of Contents
ECP201L / ECP201LG ONTENT 1. G ....................7 ENERAL NFORMATION 1.1. Product Function ..........................7 1.2. Produced Versions ..........................7 1.3. Accessories ............................7 2. G ................9 ENERAL PERATING NSTRUCTIONS 2.1. Safety Symbols on Unit ........................9 3. D ......................... - Page 4 ECP201L / ECP201LG 6.2.3.2. Valves Used ............................22 6.2.3.3. Auto Valves Closing .......................... 22 6.2.4. Compensation ..........................22 6.2.4.1. Compensation Selection........................22 6.2.4.2. Pressure Pulsation Limit ........................23 6.2.5. Solvents ............................23 6.2.5.1. Solvents Limit ............................ 23 6.2.5.2. Solvents Limit Use ..........................23 6.2.6.
- Page 5 ECP201L / ECP201LG 6.5.2.1. Main Board ............................43 6.5.2.2. Display Board ............................ 43 6.5.2.3. Communication Module ........................43 6.5.3. Ethernet ............................43 6.5.4. User Text ............................ 43 6.5.5. Pump ............................43 6.5.6. Parts ............................43 6.5.6.1. Seals ..............................44 6.5.6.2. Check Valves ............................ 44 6.5.6.3.
- Page 6 ECP201L / ECP201LG 9. RS232 .......................... 58 10. R ..................58 EPLACEMENT OF 11. T ...................... 59 ROUBLESHOOTING 12. M ......................61 AINTENANCE 12.1. Periods............................. 61 12.2. Cleaning and Decontamination ..................... 62 12.3. Cleaning Check Valves ........................62 12.4. Storage and Transport ........................62 13.
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Page 7: General Information
(ACMG000X), works also as a gradient pump. If the box is disconnected, the unit informs about the disconnection when entering menu items designated for gradient mode (e. g. Menu/Parameters/Concentration). 1.2. Produced Versions Cat. No.: ACM0000X ECP201L Preparative HPLC Pump Cat. No.: ACMG000X ECP201LG Gradient Preparative HPLC Pump 1.3. Accessories ASIC CCESSORIES Qty. - Page 8 ECP201L / ECP201LG Qty. Picture Description 990395 Open End Wrench 3/8" (for metal through bolts on capillaries) 99038900 Open End Wrench OK 11-12 (for mounting/dismounting of check valves) SCK43001 FEP inlet tubing 3/8” 2000 mm (FEP tubing,OD=3/8”, ID=1/4”, volume=63.3 ml only tubing, 1x SS nut 3/8”, 1x PTFE ferrule 3/8", thread UNF 5/8”-18)
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Page 9: General Operating Instructions
ECP201L / ECP201LG 2. G ENERAL PERATING NSTRUCTIONS Caution: If the unit is used in a manner not specified by the manufacturer, the protection provided by the manufacturer may be impaired. Caution: The unit must not be used, if it is leaking. -
Page 10: Description
ECP201L / ECP201LG 3. D ESCRIPTION RONT Description Front switch. Keyboard. Status LED lights: PUMP (green) indicates status of pump motor (PUMP and PURGE). RMT (yellow) indicates status of unit remote control. It lights when the unit is communicating through interface ETHERNET or RS232. - Page 11 ECP201L / ECP201LG EYBOARD UTTONS AND THEIR UNCTION Symbol Description MENU Entering unit menu, fast menu leaving. Pump start/stop and other functions when working with gradient program PUMP (viz. 6.3.2). FLOW Direct setting of pump flow rate. PURGE Entering purge mode.
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Page 12: Installation And First Start
ECP201L / ECP201LG 4. I NSTALLATION AND IRST TART Place the unit in a suitable location that meets the following conditions: • Horizontal place. • Keep at least 10 cm space behind the rear side of the unit. • Keep away from equipment generating strong magnetic field. -
Page 13: Outlet Capillary Connection
ECP201L / ECP201LG 4.2. Outlet Capillary Connection To connect the pump to the injection valve or column outlet capillary with stainless steel nut and ferrule (J0062600; 00000160; JR-ZF2S6) is used. Outlet capillary is connected and then the capillary nut is tightened with side wrench 3/8". -
Page 14: Automatic Backwash
ECP201L / ECP201LG 4.3.2. Automatic Backwash Connect both Washing heads in series using tubing and LUER adapters. Insert the tubing with flow rate regulator into the reservoir with HPLC water (or mobile phase dissolving buffers). Loosen the flow rate regulator and suck liquid into the system using plastic syringe connected to the other end of the system (left picture). -
Page 15: Start Of The Unit
ECP201L / ECP201LG Plug injection valve cable into START connector. Plug it all the way until it snaps in. In order to unplug the connector, press the safety pin and pull out. Insert terminal block into connector IO INTERFACE. If there is no signal connected to the terminal block, it is not necessary to connect it. -
Page 16: Basic Control By Keyboard
ECP201L / ECP201LG 4.6. Basic Control by Keyboard After switching the unit on (Mode START) initial screen displays the unit type. Then the unit turns to mode IDD, pump motor is in idle status. Main screen – isocratic pump mode. -
Page 17: Description Of Symbols On The Display
ECP201L / ECP201LG To repeatedly degass/purge the unit, it is possible to mount the waste tubing (PN000080) to the purge/degass valve. In chapter 6.2.2 it is possible to set parameters for rinsing. Now the flow rate can be set by pressing the FLOW key. -
Page 18: Control From Pc
ECP201L / ECP201LG Value/Symbol Meaning Actual piston position is displayed after first finished pump rotation. During flow rate higher than ~3 ml/min actual position cannot be displayed and this symbol is shown. Pulsation compensation status: OFF – turned off. FIXED – according to predefined values. -
Page 19: Working With Menu
ECP201L / ECP201LG 6. W ORKING WITH Menu enables setting all parameters and functions of the unit, its diagnostics and service. MAIN MENU can be entered by pushing MENU key. In the menu it is possible to move using arrows and the ENTER and ESC keys. Any part of the menu can be exited by pressing MENU key again. -
Page 20: Pressure Limits
ECP201L / ECP201LG 6.1.2. Pressure Limits Pressure limits can be set in following submenus. 6.1.2.1. High Pressure Limit High pressure limit can be set in the range of 1.0 – 15.0 MPa (150 – 2180 psi). Minimal value of high pressure limit is 1 MPa (150 psi) higher than value of low pressure limit. Default value is 5.6 MPa (813 psi). -
Page 21: General
ECP201L / ECP201LG 6.2.1. General In this submenu pressure units, display brightness and sound signalization can be set. 6.2.1.1. Pressure Units Pressure units can be set in MPa (megapascal), PSI (pound-force per sq. inch) and BAR (bar). 1 MPa=10 bar=145 psi. Default pressure unit is MPa. -
Page 22: Gradient
ECP201L / ECP201LG 6.2.3. Gradient Gradient options are set in each submenu. This item is only available for the gradient version of the pump. 6.2.3.1. Gradient Cycles Here, the number of cycles (cam cycles) of the pump, necessary for gradient mixing, can be set. -
Page 23: Pressure Pulsation Limit
ECP201L / ECP201LG Setting Description Compensation works on learned pump run. Learned process is temporary. LEARNED 0 TMP By turning the pump off and on the learned process will be canceled.TMP (Temporary). Warnings about exceeding pulsation limit are active. Learning process is... -
Page 24: Control
ECP201L / ECP201LG 6.3. Control In submenu possibilities of pulsation compensation learning function can be set. 6.3.1. Start Compensation Learning By entering this menu item, a pulsation compensation learning process starts. Before initiation of pulsation compensation learning, the pump connects to the column and starts pumping. -
Page 25: Gradient Program
ECP201L / ECP201LG Warnings Description Reason / Solution Errors PUMP IS NOT LEARNED. COMPENSATION Proceed compensation learning IS SWITCHED OFF! process. LEARNING DID NOT MEET PULSATION Operating error. Pumping stops. LIMIT WITHIN THE TIME! DATA WERE Check the unit according to ANNULLED. -
Page 26: Steps From Program Creating To Leaving Program Mode
ECP201L / ECP201LG Steps from Program Creating to Leaving Program Mode. a) Creating program in EDIT PROGRAM (see 6.3.4.3). X. …program sequence number, S…Step, xx…total number of steps, EMPTY…empty. b) Saving the program. Then the program is available in VIEW PROGRAM (see 6.3.4.2) and LOAD PROGRAM (see 6.3.4.1). -
Page 27: Init Program
ECP201L / ECP201LG Value/Symbol Meaning Program sequence number zz. ►, II, I►, Program status and its influence on other unit functions is described below. Xth step from y steps. When setting the termination of the STOP program, y Stps (Stps…Steps) is displayed. - Page 28 ECP201L / ECP201LG Action Description The change from I► to ► will be displayed on the screen, the total time Program start and step time will start. The startup method is described above. a) Pressing the PUMP key Intercepting of On display is shown change from ►...
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Page 29: Accelerated Option Of Program Initialization By Pressing The Pump Key
ECP201L / ECP201LG Action Description RUN – the program stops, the pump runs, ► changes to Program termination , total time is counting up and step time changes to End. The pump retains the setting from the last program step. -
Page 30: Status After Unit Turning Off And On
ECP201L / ECP201LG Status after Unit Turning Off and On If the unit is turned off during a running program (e.g. due to a power failure), the program is set to the pause state of the running program when the device is turned on (see table above) (►... -
Page 31: View Time Table
ECP201L / ECP201LG View Time Table Sequence number of step, time (min:s) of the step start, flow rate (ml/min), mobile phase concentration A, B, C, D in % of volume. Use up and down arrow to move between the table pages. - Page 32 ECP201L / ECP201LG It is recommended to set at first menu item VALVE USED and other functions Note: before setting TIME TABLE. Note: It is recommended to save the program after setting all steps. Saving of the program is proceeded after leaving the program by pressing the ESC key – a window menu with question save YES by pressing the ENTER key or NO by pressing the ESC key.
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Page 33: Time Table
ECP201L / ECP201LG Setting Description valve settings. No valve selection – on the screen displays: In TIME TABLE, c is displayed in all steps and in columns %A, %B, %C, %D. The running program works with the current valve settings and their concentrations (see 6.2.3.2, 6.1.3). - Page 34 ECP201L / ECP201LG Action Description 2) Adjust the start time of step X to the desired start – ENTER – step remains on the same position in the list. 3) Exit TIME TABLE – ESC. 4) Controlling the change made – enter TIME TABLE. Step X. is moved to its position in timetable of the step beginnings.
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Page 35: Modifications Of Existing Gradient Programs
ECP201L / ECP201LG The creation of a step change of the concentration in the already created TIME Example: TABLE, when the new step should be listed before the start time of the already created step, is performed by creating a new step with a start time 1 s lower than the next step. -
Page 36: Graphic Presentation Of Modifications In Running Step
ECP201L / ECP201LG program. Program concludes according to new program. Program concludes according to setting. Program does not have to be reloaded. original setting. By reloading the program in menu item LOAD PROGRAM, the changes are applied. Note: We do not recommend to combine saving changes with/without the running program modifications. -
Page 37: Exit Program Mode
ECP201L / ECP201LG In time 1:00, the gradient concentration is changed, which is shown by a change of the curve direction from the point of change. By the end of step 0, the desired concentration is set. In time 3:53, the start time of step 3 t3´ is shifted, which is shown by a change in the direction of the curve of the linear concentration gradient course starting from the point of change and with the concentration setting until the end of the step 2 according to the table. -
Page 38: Io Config
Current setting can be found in Menu/Info/Ethernet. Special application EccmConf can be used to search and manage device IP setting. The application can be downloaded from ECOM website. Search icon can be used to find all adequate units connected to the network. If no unit was found, check the unit power supply and ethernet connection (LEDs on connector should shine). - Page 39 ECP201L / ECP201LG ONNECTION STATUS Note state Description Online Connection is OK and the unit is ready for communication. Used Connection is OK, but communication cannot be opened. The unit is used already. Connection is invalid. The unit IP address doesn’t correspond to a local...
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Page 40: Dhcp/Static Ip
ECP201L / ECP201LG 6.4.1.1. DHCP/STATIC IP The choice of DHCP enables automatic setting of connecting parameters (IP address, Subnet Mask, Defaul Gateway) in network, where this function is supported. Allocation of address can take several seconds. Actual connecting parameters can be found in Menu/Info/Ethernet. - Page 41 ECP201L / ECP201LG IO I NTERFACE PECIFICATION Abb. Name Description Auxiliary power supply +5 V DC max. 40 mA. AIN− ANALOG INPUT Analog input ground. Internally connected with GND and chassis. AIN+ Analog input signal. 0 - 10 V DC. Overvoltage protection up to 12 V.
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Page 42: Analog Input
ECP201L / ECP201LG 6.4.3.1. Analog Input ANALOG INPUT can be used as follows: ETTING ODES OF NALOG NPUT Setting Description Analog input does not have any assigned function. Default setting. FLOW CONTROL Analog input is used for setting flow rate. 0 V = 0 ml/min and 10 V = 1000 ml/min. -
Page 43: Info
ECP201L / ECP201LG Setting Description digital input on OFF. COPY DIN2 If the digital input 2 is on level L, output is connected. Even in case of setting digital input on OFF. 6.5. Info In each submenu information about the unit and its parts is displayed. -
Page 44: Seals
ECP201L / ECP201LG 6.5.6.1. Seals L. PUMPING - left working head seal, R. PUMPING - right auxiliary head seal, L. WASHING - left washing head seal, R. WASHING-right washing head seal. 6.5.6.2. Check Valves L INLET – left inlet check valve, R INLET – right inlet check valve, L OUTLET – left outlet check valve, R OUTLET –... -
Page 45: Warnings
ECP201L / ECP201LG 6.6.2. Warnings WARN is displayed on the screen. If a deviation from standard unit behavior occurs, symbol W with a number, is displayed on the basic sreen, meaning number of warnings. Access to description and further behavior similar to errors, see previous the chapter. -
Page 46: Pump
ECP201L / ECP201LG 6.6.7. Pump In this menu item status of pump mechanism is displayed. Units for pressure are stated in upper row. TATUS OF ECHANISM Item Description PACT Actual pressure. PAVG Middle (integral) pressure during one cycle. Pressure pulsation (bandwidth PMAX – PMIN) per one cycle. -
Page 47: Gradient State
ECP201L / ECP201LG 6.6.10. Gradient State In this menu item values for controlling gradient valve are displayed. TATUS OF GRADIENT VALVE Item Description FUNCT Connection with the box: ON =connected, OFF = disconnected. VALVE Used valve. IDRES Measured value of the identification resistor. -
Page 48: Seals
ECP201L / ECP201LG Pistons). The unit maintenance and terms for the replacement of individual unit components are stated in chapter 12.1. Other replaceable parts are needle and O-ring in degassing/purging valve. This exchange is not recorded by the device. Caution: Before and after parts replacement, print and file Info sheet from ECOMAC or from Clarity. - Page 49 ECP201L / ECP201LG Pull the head straightly out of the piston. Leaning the head to sides may cause a piston damage! Detail of working head. Remove the seal using a suitable tool. Wash and clean the piston and interior of the head with isopropanol.
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Page 50: Check Valves
ECP201L / ECP201LG set pressure at 10 MPa. Let the pumping for at least 30 min (optimally 2h). Keep the flow rate at 1000 ml/min and set pressure at 14 MPa. Let the pumping for at least 10 min (optimally 30 min.). - Page 51 ECP201L / ECP201LG To exchange the inlet valve, loosen its holder using side wrench 12 mm and remove the holder with the inlet valve. Take the inlet valve from the holder and replace it with a new one. Loosen the capillary on the outlet valve holder using side wrench 3/8"...
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Page 52: Pistons
ECP201L / ECP201LG 6.7.1.3. Pistons Replacement of a piston can be performed only by a qualified person. 6.7.1.4. Needle of Degassing/Purging Valve Disconnect the unit from power source!!! Unscrew the old needle and replace it with a new one. 6.7.1.5. O-ring in Degassing/Purging Valve... -
Page 53: Pressure Sensor
ECP201L / ECP201LG ALIBRATIONS HART 6.7.2.1. Pressure Sensor In this menu item the zero of pressure sensor is calibrated. For user is available only item OFFSET AUTO, where pressure sensor zero is set automatically. Calibration is proceeded on stopped pump. At first loosen purge/degassing valve and then press the ENTER key for proceeding automatical calibration. -
Page 54: Selection Of Volumetric Flask Volume
ECP201L / ECP201LG HART OF YSTEM FOR LOW RATE ALIBRATION 1.Container with liquid; 2. Pump; 3. Column (CAUTION: some are not intended for pure water); 4. Pressure control valve (may be used instead of the column); 5. End of a capillary, 6. Volumetric flask. - Page 55 ECP201L / ECP201LG 3/6) The unit is prepared for start by pressing the ENTER key. At the same time start capturing mobile phase in the volumetric flask. 4/6) Time and volume start to countdown. After filling volumetric flask, press the ENTER key.
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Page 56: Checking Flow Rate
ECP201L / ECP201LG Checking Flow rate After check valve or seals replacement or after significant change of working conditions, check real flow rate first without using menu item FLOW AUTO. The mobile phase is collected into volumetric flask and time is measured with a stopwatch. -
Page 57: Fan Mode
2 meters for connection. The driver ECOM Instrument VCP needs to be installed on the PC. It creates virtual serial port, which should be available for the device (see Windows Device Manager). The driver is part of ECOMAC software or it can be downloaded from our website. -
Page 58: Rs232
ECP201L / ECP201LG The unit communicates based on communication protocol which is available upon request. 9. RS232 The unit enables communication via line RS232. On the unit is connector Canon DB9 male and on the cable must be Cannon DB9 female connector. For connecting must be used crossed serial cable, containing Canon DB9 female connectors on both sides. -
Page 59: Troubleshooting
ECP201L / ECP201LG Insert the fuse holder back. Push the fuse holder all the way inside. 11. T ROUBLESHOOTING Situation Caused by / Conditions Solution Liquid leaking from washing Damaged seal in a pumping Replace the seal heads. head. immediately. The pump must not be used with damaged seal. - Page 60 ECP201L / ECP201LG Situation Caused by / Conditions Solution Flow rate is lower than set Pressure stability. Problems with lower flow rate value. occur and usually relate to problems with pressure stability – see solution above. Solution agent. Solution agents with very high or very low viscosity may cause these problems.
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Page 61: Aintenance
ECP201L / ECP201LG Situation Caused by / Conditions Solution W 22; E 42 - SOLVENT In isocratic mode. UNDER LIMIT! Reported according to setting in 6.2.5.2. Operating error. Add mobile phase into reservoir and set new volume in menu item Menu/Parameters/Solvents volume. -
Page 62: Cleaning And Decontamination
ARTS Picture Description 58606000 GL45 Media/Storage Bottle 1000mL (storage bottle for piston back washing set, for ECP201L and ECP201LG) 00519 Piston seal 20 mm UHMWPE (working head seal; polyethylen with high molecular weight; lower chemical resistance; high abrasion resistance, for... - Page 63 Strain gauge pressure sensor (1/4-16 BSPP, 10 mV/V, 600 bar, for ECP201L and ECP201LG) ACM23000M Motor with a pinion (when ordering, note unit serial number and pump type, for ECP201L and ECP201LG) 990673 Timing belt HTD8 60 teeth, 20 mm wide...
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Page 64: Warranty And Post -W
Timing belt HTD5 105 teeth, 9 mm wide (for ECP201L and ECP201LG) ACM70000 Dita + Ervin + Grace-P+ Paul Boards (when ordering, note unit serial number and pump type, for ECP201L and ECP201LG) MV101P1A Oliver24 Board (Display Board, for ECP201L and ECP201LG) -
Page 65: Technical Parameters
ECP201L / ECP201LG 15. T ECHNICAL ARAMETERS Parameter ECP201L 2 – 1000 ml/min Flow rate Pumping system two plungers dia. 20 mm connected in parallel Maximum operating pressure 15 MPa (2180 psi*, 150 bar) up to 1000 ml/min Flow rate setting... -
Page 66: Declaration Of Conformity
Application of the product: The equipment is used in the lab for the injection of sample, switching mobile phase delivery from different pumps, sample collecting during chromatographic analysis Manufacturer: ECOM spol. s r.o., Trebonicka 239, CZ-252 19, Co. ID No.: 41192192 The following technical standards were applied when evaluating conformity: EN 61010-1 ed.2:2011 + A1:2019 (idt IEC 61010-1:2010+A1:2016+COR1:2019),... -
Page 67: Appendix 2 - Ecp201Lg G
ECP201L / ECP201LG 2 – ECP201LG G 17. A PPENDIX RADIENT REPARATIVE Gradient preparative pump with a high pressure of 15 MPa at a flow rate up to 1000 ml/min. 17.1. Accessories ASIC CCESSORIES Qty. Picture Description SCK43001 FEP inlet tubing 3/8” 2000 mm (FEP tubing,OD=3/8”, ID=1/4”, volume=63.3 ml only tubing, 1x... - Page 68 ECP201L / ECP201LG Description Status LED lights: PUMP (green) indicates status of pump motor (PUMP and PURGE). RMT (yellow) indicates status of unit remote control. It lights when the unit is communicating through interface ETHERNET or RS232. ERR (red) indicates error status of the unit. It lights, if an error is indicated and blinks, if a fatal error is indicated, when unit status has been changed.
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Page 69: Inlet Tubing Connection
ECP201L / ECP201LG Description Combined power socket with main switch and main fuse. Connector ECB2000 with cable (gradient box connection). Connector RS232. Connector Ethernet/LAN. Connector USB. Connector for external start. Connector IO Interface. 17.3. Inlet Tubing Connection At first dismount plug on the gradient box and pump inlet. -
Page 70: Technical Parameters
ECP201L / ECP201LG 17.4. Technical Parameters Parameter ECP201LG Preparative Pump 2 – 1000 ml/min Flow rate Pumping system two plungers dia. 20 mm connected in parallel Maximum operating pressure 15 MPa (2180 psi*, 150 bar) up to 1000 ml/min Flow rate setting... - Page 71 ECP201L / ECP201LG Parameter ECP201LG Operational environment Indoor use only. conditions Altitude: up to 2000 m Temperature: 5-40°C Humidity: maximum relative humidity 80 % for temperatures up to 31°C decreasing linearly to 50 % rel. humidity at 40°C. Voltage fluctuations: up to ±10 % of nominal voltage.
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