Ecom ECP2050 User Manual
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Ecom ECP2050 User Manual

Preparative hplc pump
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ECP2050
User´s Guide
ECP2050
P
HPLC
REPARATIVE
PUMP
ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic
tel: +420 221 511 310, fax: +420 242 498 212, info@ecomsro.cz, http://www.ecomsro.com/
IČO: 411 92 192, DIČ: CZ411 92 192

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  • Page 1 ECP2050 User´s Guide ECP2050 HPLC REPARATIVE PUMP ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic tel: +420 221 511 310, fax: +420 242 498 212, info@ecomsro.cz, http://www.ecomsro.com/ IČO: 411 92 192, DIČ: CZ411 92 192...
  • Page 2 ECP2050 Document properties File: Manual-ECP2050-en-03.doc Number of pages: File size: 6448 kB 8/5/2021 8:48:00 AM Kateřina Kněžourková Last save: Ver. Date Author Approved by Description Kateřina Kněžourková Jaroslav Formánek 26.06.2019 First issue. Dominika Pavlová Jaroslav Formánek 05.11.2019 Address change in the footage of the first page, the in chapters 13 and 15.

  • Page 3: Table Of Contents

    ECP2050 ONTENT 1. G ....................6 ENERAL NFORMATION 1.1. Product Function ..........................6 1.2. Product Version ..........................6 1.3. Accessories ............................6 2. G ................8 ENERAL PERATING NSTRUCTIONS 2.1. Safety Symbols on the Unit ....................... 8 3. D ........................9 ESCRIPTION 4.
  • Page 4 ECP2050 6.3. Control ............................... 25 6.3.1. Start Compensation Learning ..................... 26 6.3.2. Gradient Program ........................27 6.3.3. Init/Stop/Reinit Program ......................32 6.3.4. Program Setup ........................... 32 6.3.5. Open/Close Gradient Valves ...................... 39 6.4. IO Config............................39 6.4.1. Ethernet ............................39 6.4.2.
  • Page 5 ECP2050 11.3. Cleaning Check Valves ........................60 11.4. Storage and Transport ........................60 12. S ......................61 PARE ARTS 13. W ..............62 ARRANTY AND ARRANTY EPAIRS 14. T ..................... 63 ECHNICAL ARAMETERS 1 – D 15. A ............... 64...

  • Page 6: Eneral Nformation

    (e.g. Menu/Parameters/Concentration) and pump is isocratic mode, unit informs too. 1.2. Product Version Cat. No.: ACC0000X ECP2050 Preparative HPLC pump 1.3. Accessories ASIC CCESSORIES Qty. Picture Description EKAB-011 Mains cable 10A-250V 2 m 00343122 Tube fuse T –...
  • Page 7 ECP2050 Qty. Picture Description ACA99012 Injector holder Series 2000 ACC99400 Capillary injector-column 1mm with fittings (OD=1/16“ ; ID=1mm ; l=300mm ; volume=236µL ; thread 10-32 UNF) ACC99300 Outlet capillary to injector 1mm with fittings (OD=1/16“ ; ID=1mm ; l=300mm ; volume=236µL ; thread 10-32 UNF) ACC16001 FEP tubing 1/8“...

  • Page 8: General Operating Instructions

    ECP2050 2. G ENERAL PERATING NSTRUCTIONS Caution: If the unit is used in a manner not specified by the manufacturer, the protection provided by the unit may be impaired! Caution: The unit may not be used, if it is leaking.

  • Page 9: Description

    ECP2050 3. D ESCRIPTION RONT Description Front switch. Keyboard. Connection for input tubing (pump inlet) – working block with pumping and washing head (behind the pumping head). Connection for output capillary (pump outlet). Washing/degassing (bypass) valve – compensating block with pumping and washing head.
  • Page 10 ECP2050 EYBOARD UTTONS AND THEIR UNCTION Symbol Description MENU Entering unit menu, fast menu leaving. PUMP Pump start/stop and other functions when working with gradient program (see 6.3.2). FLOW Direct setting of pump flowrate. PURGE Entering purge mode. ENTER Confirms selected MENU item, confirms newly set parameter value, evokes QUICK DIAGNOSTICS screen.

  • Page 11: Installation And First Start

    ECP2050 4. I NSTALLATION AND IRST TART Place the unit in a suitable location that satisfies the following conditions: • Horizontal place. • Keep at least 10 cm space behind the rear of the unit. • Keep away from equipment generating strong magnetic field.

  • Page 12: Injector Holder And Column Holder Mounting

    ECP2050 Connect outlet capillary. Tighten capillary nut with side wrench 1/4" Connect the other end of capillary to injector valve. 4.3. Injector Holder and Column Holder Mounting Use Torx screwdriver TX20 to unscrew two screws in front part of the right panel of the unit.

  • Page 13: Piston Backwash

    M4 x 10 mm, using imbus key 3 mm. Screws and imbus key are among the injection valve accessories if it is ordered in Ecom spol. s r.o. Handle of injection valve is mounted by snapping into the valve body.

  • Page 14: Simple Manual Use

    ECP2050 ISTON ACKWASH 1. Inlet part; 1a. Luer cone for connecting to washing head; 1b. Flowrate regulator; 1c. Lid GL45 with plug; 1d. Luer cone for connecting syringe; 2. Connection of washing heads; 3. Outlet into waste system; 4. Syringe 50ml 4.5.1.

  • Page 15: Assemblage Of Waste System

    ECP2050 4.6. Assemblage of Waste System he pump is equipped with waste channel, leading the liquid off the pumping block. In its proximity is leakage sensor. The function of the sensor is closely described in chapter 6.2.7, in chapter 6.4.3.4 is described the setting of IO INTERFACE Digital Out Switch – LEAKAGE signalizing the leakage of pump to another unit.

  • Page 16: Connection On Unit Rear Panel

    ECP2050 Shorter tubing of part 2 (picture above) should be connected between the pump and the backstop on holder of injection valve. This tubing serves as waste tubing from washing head, put through window in valve holder. The opening of part 2 should be connected with connector L, forming a 90°...

  • Page 17: Start Of Unit

    ECP2050 Plug injection valve cable into START connector. Plug it all the way in until it snaps in. In order to unplug the connector, press the safety pin (see arrow on right picture) and pull out. Insert terminal block into connector INTERFACE, it is among the pump accessories.

  • Page 18: Basic Control By Keyboard

    ECP2050 4.9. Basic Control by Keyboard After unit start (mode START) display shows unit type. Then the unit turns to mode IDD, pump motor is in idle status. Main screen – isocratic pump mode. Main screen – gradient pump mode.

  • Page 19: Description Of Symbol On The Screen

    ECP2050 the pump. For fast setting of one of the valves to 100%, it is possible to use arrow with the letter of the appropriate valve. this switching is functional in gradient mode during modes IDD, PRG a PMP (see 7).

  • Page 20: Door Installation

    ECP2050 Value/Symbol Meaning Indication of leakage, at the same time may be displayed an error or warning (according to setting). One IO INTERFACE inputs are used for pump control. An error has occurred „ERROR“ (number of errors). By using key ENTER...

  • Page 21: Working With Menu

    ECP2050 6. W ORKING WITH Menu enables setting all parameters and functions of the unit, its diagnostics and service. MAIN MENU is entered by pushing key MENU. In the menu you can move using arrows, ENTER and ESC. Any part of the menu can be abandoned by pressing key MENU. For better orientation in menu is in next chapter shown menu flow diagram and description of menu parts.

  • Page 22: Concentration

    ECP2050 6.1.2.1. High Pressure Limit High pressure limit can be set in the range 1.0-30.0 MPa (150-4350 psi). Minimal value of high pressure limit is 1 MPa (150psi) higher than value of low pressure limits. Default value is 24 MPa (3480 psi).

  • Page 23: Purge

    ECP2050 6.2.1.2. Display Brightness Display brightness can be set in 8 level where 8 is maximum brightness. Default value is 3. 6.2.1.3. Sound Sound signals of the unit can be set as follows: OUND ETTING Setting Description KEYBOARD At each touch of button will be generated short beep when...

  • Page 24: Compensation

    ECP2050 6.2.3.3. Auto valves closing Here can be set possibilities of auto gradient valves closing after unit start and during every pump stopping. Possibilities of setting are ENABLE (auto valves closing is enabled) or DISABLE (auto valves closing is disabled). Default setting is ENABLE, after pump start is one of the valves automatically opened according to setting of used valves (see 6.2.3.2) and their concentration...

  • Page 25: Solvents

    ECP2050 6.2.5. Solvents In each sub menu are set possibilities to watch residual volume of mobile phase. 6.2.5.1. Solvents Limit Limit of mobile phase residual volume can be set in range 1-10000 ml. Default value is 100 ml. 6.2.5.2. Solvents Limit Use In this menu item can be set desired mode of warning regarding insufficiency of mobile.

  • Page 26: Start Compensation Learning

    ECP2050 6.3.1. Start Compensation Learning By entering this menu item, a pulsation compensation learning process starts. Before initiation of pulsation compensation learning, pump connects to column and starts pumping. It is ideal to carry out the learning process under working conditions, including used tubing, column etc.

  • Page 27: Gradient Program

    ECP2050 Warnings Description Reason / Solution Errors LIMIT WITHIN THE TIME! DATA WERE Check the unit according to ANNULLED. instructions in chapter 10. LEARNING WAS INTERRUPTED BECAUSE Operating error. Pumping stops. OF WORSENING! DATA WERE Check the unit according to ANNULLED.
  • Page 28 ECP2050 Steps from Program Creating to Leaving Program Mode a) Creating program in EDIT PROGRAM (see 6.3.4.3). X. …program sequence number, S…Step, xx…total number of steps, EMPTY…empty. b) Saving program. Then the program is available in VIEW PROGRAM (see 6.3.4.2) and LOAD PROGRAM (see 6.3.4.1).
  • Page 29 ECP2050 Value/Symbol Meaning Program sequence number zz. ►, II, I►, Program status and its influence on other unit functions are described below. Step x from y steps. When setting program termination STOP, displays y Stps (Stps…Steps). Total time Time status (min:s) from program start is placed in right top corner of screen.
  • Page 30 ECP2050 Action Description a) Pressing PUMP Intercepting of On display is shown change from ► to II, total time stops and flashes, running program step time stops, pump stops. Mobile phase concentration change stops. After pressing key PUMP or D again, program restarts.
  • Page 31 ECP2050 Action Description RUN – program tops, pump runs, ► changes to Program termination , total time is counting up and step time changes to End. Pump keeps setting from last program step. STOP – program terminates, pump stops, ► changes to...

  • Page 32: Init/Stop/Reinit Program

    ECP2050 6.3.3. Init/Stop/Reinit Program For controlling initialization /stop/reinitialization of program. PTIONS OF Setting Description INIT PROGRAM program initialization. Displays in menu after loading program from menu item LOAD PROGRAM (see 6.3.4.1) after program termination. STOP PROGRAM Program stopping. Displays in menu only during initialization phase of a program.
  • Page 33 ECP2050 View parameters Setting Description STEPS Total number of program steps. TIME Total program time. PLMIN Low pressure limit. PLMAX High pressure limit. VALVE Used valves A, B, C, D. CYCLE Gradient cycles. FLOWC Flow control. ENBL = enabled or DISA = disabled.
  • Page 34 ECP2050 ETTABLE ARAMETERS Setting Description TIME TABLE Setting parameters for program steps. Use arrows left/right to select parameter, press ENTER to enter the editing window: STP = step number 0-49. • 0…initialization phase. • Adding step by using arrow down from last step.
  • Page 35 ECP2050 Setting Description COPY Copying program to following positions. Press key ENTER to enter an item. Use cursor to set number of position Y. EMPTY. Press ENTER again to start copying and this screen is automatically left back to edited program X. S(xx).
  • Page 36 ECP2050 Action Description Change displays on main screen as follows: Creating a step change of concentration in already created TIME TABLE, where Example: new step should be listed in prior to time of already created step, is achieved by creating new step with start time 1 s lower then next step. Set desired concentration and flowrate.
  • Page 37 ECP2050 Only the flowrate can be modified directly by pressing key FLOW in initialization phase and other steps. When setting FLOW CONTROL – ENABLE alteration made is applies only to given step and is not saved into the program. The following step runs according to program setting.
  • Page 38 ECP2050 Graphic Presentation of Modification is Running Step In TIME TABLE is stated default program setting. During running program will be done two modifications. First modification is change of gradient concentration, proceeded during step 0 from %A = 70 to %A´ = 90. Second is modification of start time of step 3, done during step 2 from t3 = 5 min to t3´= 4 min.

  • Page 39: Open/Close Gradient Valves

    ECP2050 6.3.5. Open/Close Gradient Valves This item enables to open valves (A, B, C, D) manually when the pump is stopped, respectively it enables to close open valves when the pump is not running. It is used during pump service, when it is necessary to disconnect the flow system, where mobile phase flows by gravity.

  • Page 40: Remote Keylock

    ECP2050 Socket for connecting Ethernet is on rear panel of the unit and has two signalization diodes: Location Description LED1 Meaning: OFF = no connection; orange = 10Mbps; green = 100 Mbps. LED2 Meaning: OFF = no activity; orange = half-duplex mode; green = fully duplex mode.

  • Page 41: Interface

    ECP2050 EYLOCK ETTING Setting Description LOCKED Keypad is fully locked. ALLOW PUMP, PURGE Keypad is locked except keys PUMP and PURGE. ALLOW PUMP, PURGE, FLOW Keypad is locked except keys PUMP, PURGE and FLOW. UNLOCKED Keypad is fully functional. Default value.
  • Page 42 ECP2050 Diagnostics of actual IO INTERFACE status can be done in Menu/Diagnostics/Interface. For digital inputs value OPEN (H) means opened input, high level and CLOSE (L) means closed input, low level. For digital output is displayed OFF (for open switch) and ON (for closed switch).

  • Page 43: Info

    ECP2050 6.4.3.3. Digital Input 2 The same possibilities and setting as for Digital input 1. 6.4.3.4. Digital Output Switch Digital output (DIGITAL OUTPUT SWITCH) can be used as follows: ETTING CTION ON IGITAL UTPUT Setting Description The output is not controlled by any action. Default setting.

  • Page 44: Ethernet

    ECP2050 6.5.3. Ethernet Displays information about actual setting of ethernet. IP-IP address of the unit, MASK – subnet mask, GATE – default gateway, MAC – unique hardware address. 6.5.4. User Text User information about the unit. This information can be set only through PC. It serves for user description of the unit, its placement etc.

  • Page 45: Warnings

    ECP2050 more then one error. When entering this detailed screen, an actual status is displayed and it is not updated. For updating this information, it is necessary to re-enter this screen. For quick access to error and warning descriptions, the QUICK DIAGNOSTICS screen is displayed, which can be called from the main screen by pressing ENTER.

  • Page 46: Pump

    ECP2050 Item Description VRPL Voltage of pump reference sensor L. VRPH Voltage of pump reference sensor H. VSBL Voltage of box measuring sensor L. VSBH Voltage of box measuring sensor H. VRBL Voltage of box reference sensor L. VRBH Voltage of box reference sensor H.

  • Page 47: Gradient State

    ECP2050 Item Description Learning time – time lapsed from learning start. LTIM Learning flow – flowrate during learning (constant). LFLO PPOS Actual piston position. Note: After start of compensation learning (see 6.3.1) for items PUAN, PUBT displayed ---. Values of pulsation are displayed after n rotations. At this moment is learning process started.

  • Page 48: Parts Replacement

    ECP2050 Caution: Items marked with are not inaccessible for users. Only authorized person can enter this part of menu. Caution: The following operations may be done only by a qualified person. Before starting the below described procedures, disconnect the unit from power source by disconnecting main power cord.
  • Page 49 ECP2050 Pull the head straightly out of the piston. Leaning the head to sides may cause piston damage! Detail of working head. Remove the seal using a suitable tool. Wash and clean the piston and interior of the head with isopropanol.
  • Page 50 ECP2050 pump output. Put the inlet tubing and output capillary into same reservoir with solvent isopropanol: water 1:1. Set flow rate to 50 ml/min, start pumping and set pressure at 10MPa using reduction valve. Let the pumping for at least 30 min (optimal 2 h). Keep the flow rate at 50 ml/min and set pressure at 20MPa.
  • Page 51 ECP2050 Remove the holder with inlet valve. Take out inlet valve from the holder. Replace the inlet valve with a new one. Loosen capillary on outlet valve holder using side wrench 1/4" and bend it slightly. Or you can dismount the connecting capillary completely.

  • Page 52: Calibrations

    ECP2050 6.7.1.4. Needle of Degassing/Purging Valve Disconnect the unit from power source!!! Unscrew needle. Screw new needle. 6.7.1.5. O-ring in Degassing/Purging Valve Disconnect the unit from power source!!! Unscrew two screws using screwdriver TX20 from unit accessories. Take out the degassing/purging valve.
  • Page 53 ECP2050 6.7.2.1. Pressure Sensor In this menu item is calibrated zero of pressure sensor. For user is available only item OFFSET AUTO, where is pressure sensor zero set automatically. Calibration is proceeded on stopped pump. At first loosen purge/degassing valve and then press ENTER for proceeding automatical calibration.
  • Page 54 ECP2050 Example: For flowrate 1 ml/min resp. 5 ml/min resp. 10 ml/min is selected volumetric flask with volume 10 ml resp. 50 ml resp. 100 ml. Press ENTER to enter menu item FLOW AUTO. At the screen heading is displayed 1/6 with action instructions.

  • Page 55: Factory Settings

    ECP2050 6/6) Unit calculates new deviation based on measured and submitted parameters and corrects VCNT compared to VREF and records new deviation. Checking Flowrate After check valve or seals replacement or after significant change of working conditions, check real flowrate first without using menu item FLOW AUTO. Collect the mobile phase into volumetric flask and measure time with stopwatch.

  • Page 56: Special

    ECP2050 6.7.3.3. Restore Factory Settings This menu item enables restoring factory configuration of pressure sensor calibration, flowrate and gradient. After entering menu items by pressing ENTER – PRESSURE SENSOR, FLOW (flowrate), GRADIENT – is displayed menu for confirming the restoration.

  • Page 57: Replacement Of Line Fuse

    ECP2050 9. R EPLACEMENT OF Disconnect the unit from power source!!! Using a flat-tip screwdriver release the fuse holder from its bottom. Take out the fuse holder. Replace the old line fuse. In the holder insert new fuse from unit accessories, its value is stated on unit label.
  • Page 58 ECP2050 Problem Caused by / Conditions Solution Bubbles. Check all connections on capillaries and tubing before, in and after the pump. Try to purge the pump again and watch tubing after connected mobile phase filter, for bubble formation. If any bubbles are formed there, exchange/rinse this filter.
  • Page 59 ECP2050 Problem Caused by / Conditions Solution E28 – PRESSURE UNDER Operating error. Locate and LOW LIMIT! eliminate reason for pressure suppression – leakage from the system (leaking purge/degas valve and other, above mentioned). W4, E29 – PUMP LEAKAGE! Reported according to setting REMOVE ALL LEAKS.

  • Page 60: Maintenance

    ECP2050 Problem Caused by / Conditions Solution W13, 14, 21, 24, 25, 30; E44, Pulsation compensation 45, 51. learning, see 6.3.1. Other Contact service center. Save log and info list from Ecomac or Clarity. 11. M AINTENANCE 11.1. Periods Minimal period for check valve and seal exchange is one year. This period may be shorter if there are used buffers, high pressure/flow rate, aggressive solvents, everyday use or other aggravating conditions.

  • Page 61: Spare Parts

    ECP2050 12. S PARE ARTS Picture Description ACC16100 PE Filter 20u No-Met with reduction UNF1/4" 99043600 Seal 3/8" U-R325 HB (CC)-204-GFP-HST (head seal; Teflon filled with graphite; high chemical resistance; lower wear resistance) 99043700 Seal 3/8" U-325 MB-204-T-316 (PTFE seal of washing head) 99043000 Seal 3/8"...

  • Page 62: Warranty And Post -Warranty Repairs

    EPAIRS Warranty and post-warranty repairs are provided by the manufacturer or dealer organization authorized by company ECOM to do this. Repair of products in the warranty period carried out by other person than that authorized by the service organization is a reason for warranty nullification.

  • Page 63: Technical Parameters

    ECP2050 14. T ECHNICAL ARAMETERS Parameter Value 0.1 – 50.0 ml/min. Flowrate two pistons dia. 3/8” connected in parallel Pumping system Maximum operating pressure 30 MPa (4350 psi, 300 bar) ± 2 % Accuracy of flowrate (10 ml/min. 12MPa H2O) ±...

  • Page 64: Appendix 1 - Declaration Of Comformity

    Application of the product: Equipment is used in the lab for solvent delivery by chromatographic analysis. Manufacturer: ECOM spol. s r.o., Trebonicka 239, CZ-252 19, Co. ID No.: 41192192 The following technical standards were applied when evaluating conformity: EN 61010-1 ed.2:2011 + A1:2019 (idt IEC 61010-1:2010+A1:2016+COR1:2019), EN 61000-6-3 ed.2:2007+A1:2011 + Cor.1:2013 (idt: EN 61000-6-3:2007/A1:2011/AC:2012),...

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