Ecom ECP2010 User Manual
  1. Manuals
  2. Brands
  3. Ecom Manuals
  4. Water Pump
  5. ECP2010
  6. User manual

Ecom ECP2010 User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
ECP2010 / ECP2010H
User's Guide
ECP2010/ECP2010H
A
HPLC P
NALYTICAL
UMP
ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic
tel: +420 221 511 310, fax: +420 242 498 212, info@ecomsro.cz, http://www.ecomsro.com/
IČO: 411 92 192, DIČ: CZ411 92 192

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the ECP2010 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Ecom ECP2010

Summary of Contents for Ecom ECP2010

  • Page 1 ECP2010 / ECP2010H User’s Guide ECP2010/ECP2010H HPLC P NALYTICAL ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic tel: +420 221 511 310, fax: +420 242 498 212, info@ecomsro.cz, http://www.ecomsro.com/ IČO: 411 92 192, DIČ: CZ411 92 192...
  • Page 2 ECP2010 / ECP2010H Vlastnosti dokumentu File: Manual-ECP2010_ECP2010H-en-07.doc Number of pages: File size: 7065 kB Last save: 11/15/2021 1:46:00 PM Dominika Pavlová Ver. Date Author Approved by Description 17.12.2013 Jakub Hodaň Jaroslav Formánek First issue. 07.06.2018 Kateřina Kněžourková Jaroslav Formánek Second issue.

  • Page 3: Table Of Contents

    ECP2010 / ECP2010H ONTENT 1. G ....................7 ENERAL NFORMATION 1.1. Product Function ..........................7 1.2. Produced Versions ..........................7 1.3. Accessories ............................7 2. G ................9 ENERAL PERATING NSTRUCTIONS 2.1. Safety Symbols on the Unit ....................... 9 3. D .........................
  • Page 4 ECP2010 / ECP2010H 6.2.2.1. Purge Flow ............................25 6.2.2.2. Pressure Limit ........................... 25 6.2.2.3. Purge Time ............................25 6.2.3. Gradient ............................26 6.2.3.1. Gradient Cycles ..........................26 6.2.3.2. Valves Used ............................26 6.2.3.3. Auto Valves Closing .......................... 26 6.2.4. Compensation ..........................26 6.2.4.1.
  • Page 5 ECP2010 / ECP2010H 6.4.3.4. Digital Output Switch ......................... 44 6.5. Info ..............................45 6.5.1. Device ............................45 6.5.2. Boards ............................45 6.5.2.1. Main Board ............................45 6.5.2.2. Display Board ............................ 45 6.5.2.3. XPORT Module ..........................45 6.5.3. Ethernet ............................45 6.5.4.
  • Page 6 ECP2010 / ECP2010H 7. W ......................58 ORKING ODES 8. RS232 .......................... 58 9. R ..................58 EPLACEMENT OF 10. T ...................... 59 ROUBLESHOOTING 10.1. Diagnostics from PC Record ......................61 Correct Pressure ............................61 Released Bubble ............................62 Damaged Check Valves ..........................62 Damaged Seals ..............................

  • Page 7: Eneral Nformation

    Together with box, supplied separately, works also as gradient pump as well as an isocratic pump. If the box is disconnected, the unit informs about its disconnection when entering menu items designated for gradient mode (e.g. Menu/Parameters/Concentration). 1.2. Produced Versions ECP2010 Analytical HPLC pump 40Mpa Cat.: ACA0000X ECP2010H Analytical HPLC pump 60MPa Cat.: ACF0000X...
  • Page 8 Syringe LUER 10 ml, plastic ACA99012 Injector holder Series 2000 (combined holder for injecting valves of Rheodyne and Vici type) ACA99700 Back washing set for ECP2010 ACA99000 Waste set ECP2010 ACA99020 Tool for 1/8" removal (for replacing seals in heads)

  • Page 9: General Operating Instructions

    ECP2010 / ECP2010H 2. G ENERAL PERATING NSTRUCTIONS Caution: If the unit is used in a manner not specified by the manufacturer, the protection provided by the unit may be impaired! Caution: The unit may not be used, if it is leaking.

  • Page 10: Description

    ECP2010 / ECP2010H 3. D ESCRIPTION RONT Description Front switch. Keyboard. Connection for input tubing (pump inlet) – working block with pumping and washing head (behind the pumping head). Connection for output capillary (pump outlet). Washing/degassing (bypass) valve – compensating block with pumping and washing head.
  • Page 11 ECP2010 / ECP2010H EYBOARD UTTONS AND THEIR UNCTION Symbol Description MENU Entering unit menu, fast menu leaving. PUMP Pump start/stop and other functions when working with gradient program (viz. 6.3.2). FLOW Direct setting of pump flowrate. PURGE Entering purge mode.

  • Page 12: Installation And Basic Control From Keyboard

    ECP2010 / ECP2010H 4. I NSTALLATION AND ASIC ONTROL FROM EYBOARD Place the unit in a suitable location that satisfies the following conditions: • Horizontal place. • Keep at least 10 cm space behind the rear of the unit. •...

  • Page 13: Injector Holder And Column Holder Mounting

    ECP2010 / ECP2010H Connect outlet capillary. Tighten capillary nut with side wrench 1/4 ". Connect the other end of capillary to injector valve. 4.3. Injector Holder and Column Holder Mounting Use Torx screwdriver TX20 to unscrew two screws in front part of the right panel of the unit.

  • Page 14: Piston Backwash

    ECP2010 / ECP2010H Handle of injection valve is mounted with two imbus worms, using imbus key 2 mm. The cable is connected to connector START on unit rear panel. Injection valve Vici is mounted to the holder with two screws...

  • Page 15: Simple Manual Use

    ECP2010 / ECP2010H ET FOR ISTON ACKWASH 1. Inlet part; 1a. Luer cone for connecting to washing head; 1b. Flowrate regulator; 1c. Lid GL45 with plug; 1d. Luer cone for connecting syringe; 2. Connection of washing heads; 3. Outlet into waste system;...

  • Page 16: Assemblage Of Waste System

    ECP2010 / ECP2010H 4.6. Assemblage of Waste System The pump is equipped with waste channel, leading the liquid off the pumping block. In its proximity is leakage sensor. The function of the sensor is closely described in chapter 6.2.7, in chapter 6.4.3.4 is described the setting of IO INTERFACE Digital Out Switch –...
  • Page 17 ECP2010 / ECP2010H Shorter tubing of part 2 (picture above) should be connected between the pump and the backstop on holder of injection valve. This tubing serves as waste tubing from washing head, put through window in valve holder. The opening of part 2 should be connected with connector L, forming a 90°...
  • Page 18 ECP2010 / ECP2010H Another possible setting is complete system HPLC (box, pump, column oven, detector). Each unit from the HPLC system has among its accessories all necessary parts for interconnection of entire waste system. On picture below is shown complete waste system and detailed picture of the pump.

  • Page 19: Connections On Unit Rear Panel

    ECP2010 / ECP2010H 4.7. Connections on Unit Rear Panel Plug the power supply cord into the instrument socket on unit rear panel. The cord is among unit accessories. Plug the interconnecting cable between pump and box into connector ECB2000. Cable is among box accessories.

  • Page 20: Start Of Unit

    (for example using a multiple socket-outlet with a switch). 4.9. Basic Control by Keyboard After unit start (Mode START) initial screen displays unit type ECP2010 / ECP2010H. Then the unit turns to mode IDD, pump motor is in idle status.

  • Page 21: Description Of Symbols On The Screen

    ECP2010 / ECP2010H Suck in the mobile phase with the plastic syringe as long as bubbles come out from the pump. It is possible to switch the valves during washing, by pressing keys with arrows, this allows the liquid to penetrate to all necessary channels Press key PUMP again to stop the pump washing.

  • Page 22: Door Installation

    ECP2010 / ECP2010H Value/Symbol Meaning Abbreviation of actual pump mode. Actual piston position is displayed after first finished pump rotation. During flowrate higher than ~3 ml/min actual position cannot be displayed and this dummy symbol is shown. Pulsation compensation status: OFF –...

  • Page 23: Control From Pc

    ECP2010 / ECP2010H 5. C ONTROL FROM During unit control from PC communication proceeds through serial line RS232, USB or through LAN. Communication protocol can be supplied upon request. Unit may be controlled with SW Clarity or ECOMAC. When using program Clarity for pump control, use last upgraded program version, including drivers for this unit.

  • Page 24: Flow Rate

    6.1.2.1. High Pressure Limit High pressure limit can be set in the range for ECP2010 / ECP2010H 1.0 - 40.0/60.0 MPa (150 - 5800/8700 psi). Minimál value of high pressure limit is 1 MPa (150 psi) higher than value of low pressure limit.

  • Page 25: General

    ECP2010 / ECP2010H 6.2.1. General In these sub menus can be set pressure units, display brightness, sounds and pump mode. 6.2.1.1. Pressure Units Pressure units can be set to MPa (megapascal), PSI (pound-force per sq. inch) and BAR (bar). 1 MPa =10 bar = 145 psi. Default pressure unit is MPa.

  • Page 26: Gradient

    ECP2010 / ECP2010H 6.2.3. Gradient Here can ge set possibilities of gradient functions. 6.2.3.1. Gradient Cycles Here can be set number of cam cycles of the pump, necessary for one gradient mixing cycle. Default setting is AUTOMATIC, when can be used one cycle with flowrate up to 2.5 ml/min, two cycles with flowrates from 2.5 up to 5.0 ml/min and 4 cycles for flowrates over 5.0 ml/min.

  • Page 27: Pressure Pulsation Limit

    ECP2010 / ECP2010H – FW 5. ULSATION OMPENSATION STARTING FROM Setting Description Compensation is turned off. Motor runs steadily independently of pressure. Warnings about exceeding pulsation limit are not active. Default value. LEARNED 0 Compensation works on learned pump run. Learned process is temporary. By turning the pump off and on the learned process is canceled.

  • Page 28: Leakage

    ECP2010 / ECP2010H 6.2.7. Leakage Leakage sensor contains reference and measuring sensor. If measuring sensor is immersed in liquid, sensor generates a signal. Processing of this signal depends on actual setting. The pump also processes the leakage sensor from connected box. Behavior setting is then common for both sensors, but descriptions of warnings and errors are different.

  • Page 29: Gradient Program

    Note: Gradient program functions is available starting from firmware (FW) verzion 4.x. Both types of pumps ECP2010 / ECP2010H enable setting of 12 gradient programs directly in the unit. Programming offers a tool for gradient concentration course control and controlling mobile phase flowrate in time.

  • Page 30: Steps From Program Creating To Leaving Program Mode

    ECP2010 / ECP2010H IARAM FOR TEMS ONTROL A ROGRAM ETUP Steps from Program Creating to Leaving Program Mode. a) Creating program in EDIT PROGRAM (see 6.3.4.3). X. …program sequence number, S…Step, xx…total number of steps, EMPTY…empty. b) Saving program. Then the program is available in VIEW PROGRAM (see 6.3.4.2) and LOAD PROGRAM (see 6.3.4.1).
  • Page 31 ECP2010 / ECP2010H When continuing work with the same program proceed the same way from paragraph d) in case of ending by END STEP – RUN, STOP or when ending during running program (see paragraph f). Proceeding from paragraph e) follows in case of END STEP – REPEAT or ending during running program by evoking menu item Menu/Control/Reinit program.
  • Page 32 ECP2010 / ECP2010H Action Description INIT PROGRAM In item Menu/Control/Init program, press ENTER. I►, Parameter setting of initialization step 0. On main screen displays total time does not count up and flashes, pump runs. On display is shown change from I► to ►, total time and step time Program start starts.

  • Page 33: Accelerated Option Of Program Initialization By Pressing Pump

    ECP2010 / ECP2010H Action Description Program termination RUN – program tops, pump runs, ► changes to , total time is counting up and step time changes to End. Pump keeps setting from last program step. STOP – program terminates, pump stops, ► changes to...

  • Page 34: Program Setup

    ECP2010 / ECP2010H PTIONS OF Setting Description INIT PROGRAM Program initialization. Displays in menu after loading program from menu item LOAD PROGRAM (see 6.3.4.1) and after program termination. STOP PROGRAM Program stopping. Displays in menu only during initialization phase of a program.

  • Page 35: View Parameters

    ECP2010 / ECP2010H View Parameters Setting Description STEPS Total number of program steps. TIME Total program time. PLMIN Low pressure limit. PLMAX High pressure limit. VALVE Used valves A, B, C, D. CYCLE Gradient cycles. FLOWC Flow control. ENBL = enabled or DISA = disabled.
  • Page 36 ECP2010 / ECP2010H ETTABLE ARAMETERS Setting Description TIME TABLE Setting parameters for program steps. Use arrows left/right to select parameter, press ENTER to enter the editing window: STP = step number 0-49. • 0…initialization phase. • Adding step by using arrow down from last step.
  • Page 37 ECP2010 / ECP2010H Setting Description COPY Copying program to following positions. Press key ENTER to enter an item. Use cursor to set number of position Y. EMPTY. Press ENTER again to start copying and this screen is automatically left back to edited program X. S(xx).

  • Page 38: Modifications Of Existing Gradient Programs

    ECP2010 / ECP2010H Action Description Change displays on main screen as follows: Creating a step change of concentration in already created TIME TABLE, where Example: new step should be listed in prior to time of already created step, is achieved by creating new step with start time 1 s lower then next step.
  • Page 39 ECP2010 / ECP2010H Only the flowrate can be modified directly by pressing key FLOW in initialization phase and other steps. When setting FLOW CONTROL – ENABLE alteration made is applies only to given step and is not saved into the program. The following step runs according to program setting.

  • Page 40: Graphic Presentation Of Modifications In Running Step

    ECP2010 / ECP2010H Graphic Presentation of Modifications in Running Step. In TIME TABLE is stated default program setting. During running program will be done two modifications. First modification is change of gradient concentration, proceeded during step 0 from %A = 70 to %A´ = 90. Second is modification of start time of step 3, done during step 2 from t3 = 5 min to t3´= 4 min.

  • Page 41: Open/Close Gradient Valves

    ECP2010 / ECP2010H 6.3.5. Open/Close Gradient Valves This item enables to open valves (A, B, C, D) manually when the pump is stopped, respectively it enables to close open valves when the pump is not running. It is used during pump service, when it is necessary to disconnect the flow system, where mobile phase flows by gravity.

  • Page 42: Dhcp/Static Ip

    ECP2010 / ECP2010H Current setting can be found in Menu/Info/Ethernet. 6.4.1.1. DHCP/STATIC IP The choice of DHCP enables automatic setting of connecting parameters (IP address, Subnet masc, Defaul gateway) in network, where this function is supported. Allocation of address can take several seconds. Actual connecting parameters can be found in Menu/Info/Ethernet.
  • Page 43 ECP2010 / ECP2010H IO I NTERFACE PECIFICATION Abb. Name Description Auxiliary power supply +5 V DC max. 40 mA. AIN− ANALOG INPUT Analog input ground. Internally connected with GND and chassis. AIN+ Analog input signal. 0 - 10 V DC. Overvoltage protection up to 12 V.

  • Page 44: Analog Input

    ECP2010 / ECP2010H 6.4.3.1. Analog Input ANALOG INPUT can be used as follows: ETTING ODES OF NALOG NPUT Setting Description Analog input does not have any assigned function. Default setting. FLOW CONTROL Analog input is used for setting flow rate. 0 V = 0 ml/min and 10 V = 10 ml/min.

  • Page 45: Info

    ECP2010 / ECP2010H Setting Description COPY DIN2 When digital input 2 is on level L, output is connected. Even in case of setting digital input on OFF. 6.5. Info In each submenu is displayed information about the unit and its parts.

  • Page 46: Seals

    ECP2010 / ECP2010H 6.5.6.1. Seals L.PUMPING - left R.PUMPING - right working head seal, auxiliary head seal, L.WASHING - left washing head seal, R.WASHING-right washing head seal. 6.5.6.2. Check Valves INLET - inlet check valve, OUTLET - outlet check valve.

  • Page 47: Power

    26 – 30 V. 6.6.4. Cooling Display shows COOL. Shows rotation speed of FAN1 and FAN2 (rpm) and their voltage (V). In pump version ECP2010 / ECP2010H is only one fan mounted, therefore for one fan is shown NOT USED. 6.6.5. Interface On screen is displayed IFACE.

  • Page 48: Pump

    ECP2010 / ECP2010H 6.6.7. Pump Displays status of pump mechanism. Units for pressure are stated in upper row. TATUS OF ECHANISM Item Description PACT Actual pressure. PAVG Middle pressure during one cycle. Pressure pulsation (bandwidth PMAX – PMIN) per one cycle.

  • Page 49: Service

    ECP2010 / ECP2010H RADIENT ALVE TATUS Item Description FUNCT Box connection: ON = connected, OFF = disconnected. VALVE Valve used. IDRES Measured value of identification resistor. No. of box type. GVHV Gradient Valve Hold Voltage 26 - 30 V (H … hight current).

  • Page 50: Seals

    ECP2010 / ECP2010H Other replaceable parts are needle and O-ring in degassing/purging valve. This exchange is not recorded by the device. Caution: Before and after parts replacement, print and file Info sheet from Ecomac or from Clarity. Caution: New parts should be rinsed in isopropanol or methanol before replacement.
  • Page 51 ECP2010 / ECP2010H To exchange the seal in (left) washing head, pull the head off directly from piston. Details of (left) washing head. Unscrew three screws using TX6 screwdriver and dismount the seal cover. Remove the seal using tool from unit accessories and insert new in the same manner as in working head.

  • Page 52: Check Valves

    ECP2010 / ECP2010H Caution: Watch washing heads and all connections during run-in for any leakage. Correct every untightness! In menu item Menu/Service/Parts replacement/Seals tick the seal that has been replaced. L PUMPING – left working head seal L WASHING – left washing head seal R PUMPING –...

  • Page 53: Pistons

    ECP2010 / ECP2010H Loosen capillary on the holder of outlet check valve using a side wrench 1/4" and slightly bend it. Eventually dismount entire connecting capillary. For exchange of outlet check valve, unscrew the holder of outlet check valve using a side wrench 5/16"...

  • Page 54: O-Ring In Degassing/Purging Valve

    ECP2010 / ECP2010H 6.7.1.5. O-ring in Degassing/Purging Valve Disconnect the unit from power source!!! Unscrew two screws using screwdriver TX20 from unit accessories. Take out the degassing/purging valve. Under head, there is white or transparent seal. Unscrew needle, taka out metal ring a O-ring.

  • Page 55: Selection Of Volumetric Flask Volume

    YSTEM FOR LOWRATE ALIBRATION 1.Container with liquid; 2. Pump (ECP2010 / ECP2010H); 3. Column (caution some are not intended for pure water); 4. Pressure control valve (may be used instead of column); 5. End of capillary, 6. Volumetric flask. Note: We recommend to use freshly degassed mobile phase.
  • Page 56 ECP2010 / ECP2010H 2/6) Parameters measured by the unit are displayed – time, volume, pressure. Let the unit to stabilize for approximately 1 minute. Press ENTER to continue. TIME --- ( --- ml); PRES xx (yy) MPa (xx…actual pressure, yy…pulsation) 3/6) Unit is prepared for start by pressing ENTER.

  • Page 57: Checking Flowrate

    ECP2010 / ECP2010H Checking Flowrate After check valve or seals replacement or after significant change of working conditions, check real flowrate first without using menu item FLOW AUTO. Collect the mobile phase into volumetric flask and measure time with stopwatch.

  • Page 58: Working Modes

    ECP2010 / ECP2010H 7. W ORKING ODES The unit may be in different working modes regarding to requested actions. It passes to some modes after command of staff or PC, others are evoked automatically in order to execute a sequence of operations.

  • Page 59: Troubleshooting

    ECP2010 / ECP2010H Replace the old line fuse. In the holder insert new fuse from unit accessories, its value is stated on unit lable. Insert the fuse holder back. Push the fuse holder all the way inside. 10. T ROUBLESHOOTING...
  • Page 60 ECP2010 / ECP2010H Problem Caused by / Conditions Solution Damaged seal – esp. when Try to replace seals using solvents as Acetonitrile. (See 6.7.1.1). It may seem like that bubbles are formed inside the pumping system, it is convenient to exclude other source of bubbles using degasser.

  • Page 61: Diagnostics From Pc Record

    ECP2010 / ECP2010H Problem Caused by / Conditions Solution W5, E30 – BOX LEAKAGE! Reported according to setting in 6.2.7. REMOVE ALL LEAKS. DRY Operating error. UP SENSOR AND WAIT Eliminate source of leaking FOR 60 s TO UPDATE. and dry up surroundings of sensor (see 6.2.7)

  • Page 62: Released Bubble

    ECP2010 / ECP2010H flowrate, the difference between high and small peak reduces. They may merge in one peak. The record course is also influenced by mobile phase and sample rate. – C RESSURE ECORD IN COMAC ORRECT OURSE 0.65 0.70 0.75...

  • Page 63: Damaged Seals

    ECP2010 / ECP2010H auxiliary block to the peak recording the work of working block. It is indicated damage of inlet check valve. – D RESSURE ECORD IN COMAC AMAGED HECK ALVE 20.3 20.2 20.1 20.0 19.9 19.8 19.7 Time [min] Flowrate 1 ml/min, 50% isopropanol s 50 % distilled water, compensation OFF In case of confusion which check valve is damaged.

  • Page 64: Cleaning And Decontamination

    ECP2010 / ECP2010H Piston replacement can be done approximately once per 5 years when conditions are hard, or when they are damaged after inappropriate replacement of other parts. When the unit is used under common conditions, replacement is done approximately once per 10 years.
  • Page 65 ECP2010 / ECP2010H Picture Description ACA70000 Outlet check valve for ECP2010 / ECP2010H (conic root of thread for connecting capillary; ceramic seat, without frit and spring) (further possibilities – ACA70100 sapphire seat without frit and spring, and other – contact sales department)

  • Page 66: Warranty And Post -Warranty Repairs

    EPAIRS Warranty and post-warranty repairs are provided by the manufacturer or dealer organization authorized by company ECOM to do this. Repair of products in the warranty period carried out by other person than that authorized by the service organization is a reason for warranty nullification.

  • Page 67: Technical Parameters

    ECP2010 / ECP2010H 14. T ECHNICAL ARAMETERS Parameter ECP2010 ECP2010H Flowrate 0.02 – 10.00 ml/min Pumping system Two plungers dia. 1/8“ connected in series Stroke volume ~62 µl main piston; ~32 µl auxiliary piston Maximum operating pressure 40 MPa (5800 psi, 400 bar)

  • Page 68: Appendix 1 - Declaration Of Conformity

    Application of the product: Equipment is used in the lab for solvent delivery by chromatographic analysis. Manufacturer: ECOM spol. s r.o., Trebonicka 239, CZ-252 19, Co. ID No.: 41192192 The following technical standards were applied when evaluating conformity: EN 61010-1 ed.2:2011 + A1:2019 (idt IEC 61010-1:2010+A1:2016+COR1:2019), EN 61000-6-3 ed.2:2007+A1:2011 + Cor.1:2013 (idt: EN 61000-6-3:2007/A1:2011/AC:2012),...

This manual is also suitable for:

Ecp2010h

Table of Contents