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Summary of Contents for MICROLIT NERO
- Page 1 ® MICROLIT NERO High Precision Micropipette Single and Multichannel (Variable & Fixed Volume)
- Page 2 TABLE OF CONTENTS: 1. Product Description 2. Product Features 3. Raw Material 4. Micropipette Operation 5. Tip Ejection 6. Pipetting Technique 7. Calibration 8. Micropipette Specifications 9. Error Calculation 10. Maintenance 11. Short Term Service 12. Long Term Service 13. Disassembly & Re-assembly of Micropipette 14.
- Page 3 Easy tip ejection: Product Description These micropipettes are designed with An in-built tip-ejector is designed ergonomic and intuitive handling, ensuring high to eject tips with optimal force. precision with its µAir® technology. They are available in Single Channel Fixed and Variable Volume ranges.
- Page 4 Pipetting Technique Reverse Technique: 1. To aspirate the liquid in the tip, press the General Instructions plunger to the second stop and immerse the 1. Press and release the plunger slowly, at all pipette tip vertically in the liquid. times, particularly when working with high viscosity reagents / solutions.
- Page 5 2. Immerse the tip into the reagent/solution and press the push button till the first stop. Make sure the tip is well below the surface. 3. Release the push button slowly to let it retract to the ready position. This will fill the tip. Keep the tip in the solution.
- Page 6 Short Term Service Multichannel Micropipettes The Multichannel Micropipette is available in 6 The micropipette must be checked at the unique volume ranges from 0.5 µl to 300 µl. beginning of each day for dust and dirt on the outer surface. Particular attention must Volume be paid to the Tip Cone.
- Page 7 Step 5: Grease the cleaned parts with a lubricant, preferably silicon grease. A2. Reassembly: (For single channel variable volume and fixed volume 5 ml and 10 mll Step 1: Place the o-ring in the tip cone and screw the o-ring seat Step 3: Rotate the tip cone in the counter-clockwise direction to separate it from the piston.
- Page 8 Step 2: Screw the piston and tip cone assembly C1. Disassembly: together at the piston holder. (For multichannel micropipettes) Step1: Hold the pipette and pull out the ejector bottom Step 3: Push the ejector bottom into the main The Tip cone area will be exposed assembly.
- Page 9 Performance Optimizatio Autoclaving Activity Action Pipette with a consistent Consistent Technique autoclaving them at 121°C (252°F) at 15 psi rhythm, pressure and speed. for a minimum of 20 minutes. Tip Size and Fit Use proper size tip, firmly placed on Tip Cone. Pre-rinse Tips Pre-rinse pipette tips for improved precision.
- Page 10 Dispense Liquid Against the Wall of the Don't Pull Out the Plunger by Force Avoid rapid pipetting movements that can Receiving Vessel When dispensing liquid, touch the pipette tip to create air bubbles and lead to inaccurate the inside wall of the receiving vessel to volume measurements.
- Page 11 Troubleshooting Calibration Tool The table below lists possible problems and their solutions. Problems Possible Reason Pipette tip does Use the appropriate not fit properly tip for exact tip onto the tip cone fitment. Tip Sample Liquid being Shorten the amount of time the liquid is pipetted is hot or Liquid is leaking...
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