Download Print this page

Applikon my-Control Operator's Manual

For autoclavable bioreactors 2 and 3 l
Hide thumbs Also See for my-Control:

Advertisement

Quick Links

my-Control
for
Autoclavable Bioreactors 2 and 3 L
OPERATOR MANUAL
Software Version mE.2.9.X; Document Version 2.9

Advertisement

loading
Need help?

Need help?

Do you have a question about the my-Control and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Applikon my-Control

  • Page 1 Autoclavable Bioreactors 2 and 3 L OPERATOR MANUAL Software Version mE.2.9.X; Document Version 2.9...
  • Page 2: Table Of Contents

    OPERATOR MANUAL CONTENTS my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 TABLE OF CONTENTS Safety ................................3 Safety Symbols ..........................3 Safety Warnings ..........................4 General................................7 General Introduction ..........................7 The User Interface ..........................7 Graphical Presentation of Buttons ....................10 System Tag / Tab presentation ......................11...
  • Page 3: Safety

    All rights reserved. This set of documents is protected by copyright law. It may not be reproduced, stored or made public in any form by any means without the prior written approval of Applikon Biotechnology B.V. Applikon Biotechnology B.V. Heertjeslaan 2...
  • Page 4: Safety Warnings

    The rear of the my-Control cabinet must be accessible. • The power switch of the my-Control is located at the rear of the cabinet. Make sure that the my-Control is installed in such a way that, in case of emergency, the power switch can be easily reached! WARNING Risk of overpressure in the glass bioreactor.
  • Page 5 In most cases, the bioreactor is equipped with a Stirrer Motor that drives the stirrer shaft and impellers. The my-Control must not be switched on until the motor cables are properly connected to both the controller and the stirrer motor.
  • Page 6 OPERATOR MANUAL SAFETY my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 Disposal instructions • This product must not be disposed of together with domestic waste. • All users are obliged to hand in all electrical or electronic devices, regardless of whether or not they contain toxic substances, at a municipal or commercial collection point so that they can be disposed of in an environmentally acceptable manner.
  • Page 7: General

    Through a network that is connected to the my-Control (TCP/IP communication), different kind of devices can be used as a User Interface (UI). Since the my-Control is addressed by using its IP address, the User Interface is also called the Web UI.
  • Page 8 Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 After switching on the power of the my-Control and invoking the Web UI with the Internet browser, the my- Control displays its Home Screen. Example of the Home Screen in View mode (no user is logged in, control loops are “Idle”): The presented Synoptic View shows a glass stirred tank reactor.
  • Page 9 • For daily operation of the controller (starting and stopping control loops and editing control parameters, the buttons and bars in the right-hand section of the screen (such as ) can be used. Comprehensive information concerning the use of the my-Control in combination with bioprocesses can be found in the corresponding Software Reference Manual!
  • Page 10: Graphical Presentation Of Buttons

    OPERATOR MANUAL GENERAL my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 GRAPHICAL PRESENTATION OF BUTTONS For the buttons in the Web User Interface, the following icons are used: Button Description Button Description Home Screen button...
  • Page 11: System Tag / Tab Presentation

    OPERATOR MANUAL GENERAL my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 SYSTEM TAG / TAB PRESENTATION In the table below, the sensor and actuator symbols (as they are presented in the right side of the Home screen)
  • Page 12: Assembling The Reactor

    The exact head plate layout depends on the reactor type (volume). For details of the available head plates and their auxiliaries, see the my-Control Hardware Manual. Assembling Instructions for the bioreactor: Assemble the bioreactor at a temperature > 15 ºC! •...
  • Page 13: Liter Reactor (Dished Bottom)

    OPERATOR MANUAL ASSEMBLING THE REACTOR my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 3.2.1 2 LITER REACTOR (DISHED BOTTOM) Port Head Plate Auxiliary M30 x 1 (central port) Top Stirrer Assembly G3/4" DO sensor holder M18 x 1.5...
  • Page 14: Liter Reactor (Dished Bottom)

    OPERATOR MANUAL ASSEMBLING THE REACTOR my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 3.2.2 3 LITER REACTOR (DISHED BOTTOM) Port Head Plate Auxiliary M30 x 1 (central port) Top Stirrer Assembly G3/4" dO2 sensor holder M18 x 1.5...
  • Page 15: Sensor

    SENSOR HOLDER FOR THE LUMISENS dO SENSOR The bioreactor systems with a my-Control come with an optical LumiSens sensor to measure the dissolved oxygen concentration in the medium. The sensor tip of the LumiSens sensor is equipped with M12 thread to fit in the sensor ports of the MiniBio reactors.
  • Page 16: Dis)Assembling The Magnetically Coupled Stirrer

    OPERATOR MANUAL ASSEMBLING THE REACTOR my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 (DIS)ASSEMBLING THE MAGNETICALLY COUPLED STIRRER Before mounting the Magnetically Coupled Stirrer Assembly, it needs to be disassembled. Remove the Stirrer Shaft by turning it counter- clockwise.
  • Page 17: Ph And Do Sensor Preparation

    The glass tube is equipped with a threaded magnetically coupling that can be separated from the sensor head. The sensor cable has a USB connector to fit in the USB port of the Applikon Controller.
  • Page 18: Factory Calibration Data For The Lumisens Sensor Tip

    Sensor Calibration Settings Part of the LumiSens Test Data Sheet Make sure to enter the factory calibration data to the LumiSens Custom Sensor Settings at the my-Control. Basically, the LumiSens dO sensor tip is now calibrated for the rest of its lifetime.
  • Page 19: Ph Sensor Calibration Routine

    SENSOR CALIBRATION ROUTINE For more details regarding the pH sensor calibration procedure, refer to the my-Control Software Reference Manual. It is advised to calibrate the sensor with two buffer solutions (pH = 7.00 and pH = 4.00). This means that a 2 point calibration is performed.
  • Page 20 OPERATOR MANUAL pH AND dO SENSOR CALIBRATION my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 Step 5: Enter the pH value of the second buffer solution (e.g. 4.00). Rinse the pH sensor and carefully wipe it dry (do not rub).
  • Page 21: Dose Monitors Initialization

    OPERATOR MANUAL DOSE MONITORS INITIALIZING my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 5 DOSE MONITORS INITIALIZATION GENERAL The actuators that are used for the addition of liquids and/or gasses can be monitored, using the Dose Monitor function.
  • Page 22: Autoclaving

    Filling the bioreactor • Fill the bioreactor with culture medium. Do not exceed the working volume of the reactor. See the my-Control Hardware Manual, Chapter “Reactors”. Be sure to leave enough space for additions after sterilization (e.g. inoculum, separately sterilized nutrients, etc.). Fasten the six mill nuts crosswise by hand.
  • Page 23: Autoclaving Procedure

    2. Place the bioreactor with all accessories in an autoclave, including the bottle rack. The space needed in the autoclave is given in the Hardware Manual, Chapter “Reactors” of the my-Control. 3. Liquid addition bottles with the appropriate solutions (extra medium, acid, base, anti-foam agent) must also be autoclaved.
  • Page 24: Preparing Cultivation

    Wrap the heating blanket around the reactor. Hook up the aeration outlet(s) (sparger / overlay) of the my-Control to the air inlet filter(s) of the reactor. Connect cooling water tubing to the condenser. Mount the stirrer motor on top of the stirrer assembly.
  • Page 25: Preparing For Operation

    Set points: • Press the button of the my-Control to display the Home screen and select the Sensor tab at the right side of the screen. • Press the setpoint value field of a control loop.
  • Page 26: Sensor

    OPERATOR MANUAL PREPARING CULTIVATION my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 CALIBRATING THE dO SENSOR If the optical LumiSens dO2 sensor is used for measuring the dissolved oxygen concentration, this section can be omitted (the sensor sensitivity is not affected by autoclaving).
  • Page 27: Resetting Dose Monitor Values

    OPERATOR MANUAL PREPARING CULTIVATION my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 Step 3: The obtained slope value for the dO sensor is presented (the offset will be set to 0.00). Press the...
  • Page 28: Cultivation

    Control with preset PID parameters • Adaptive (automatic) control For information concerning the controller settings and optimizing the control parameters, see the my-Control PID Control Manual. INOCULATION Inoculation can be performed in several ways. Two commonly used methods are described here.
  • Page 29: Tube Welding

    OPERATOR MANUAL CULTIVATION my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 8.2.1 TUBE WELDING Like other additions, adding the inoculum must be carried out aseptically. An easy and safe way of an aseptical connection of tube ends is the so-called “Tube Welding” technique.
  • Page 30: Sampling

    OPERATOR MANUAL CULTIVATION my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 SAMPLING To sample a fermentation broth, a sample pipe is needed to which a sample system is attached. Sample system Connect a syringe to the tubing that is connected to the "out barb"...
  • Page 31: Cleaning

    (standard dO sensor): Remove the LumiSens sensor head from the sensor tip and leave it near the my-Control. Remove the sensor tip from the reactor head plate and cover the tip with the End Cap (make sure that no moisture can enter the glass tube).
  • Page 32 OPERATOR MANUAL CLEANING my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 Disassembling the reactor Remove the head plate including the stirrer assembly. Remove the auxiliaries. Remove the baffles. Remove the impellers. Disassemble the magnetically coupled top stirrer assembly.
  • Page 33: Cleaning The Porous Sparger

    OPERATOR MANUAL CLEANING my-Control for Autoclavable Bioreactor Systems 2 and 3 L Software Version mE.2.9.X; Document Version 2.9 CLEANING THE POROUS SPARGER Depending on the type of medium that is used (presence of proteins and/or peptides), cleaning the porous sparger may require a special procedure.
Save PDF