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Dhyana 401D
User Manual
V1.0.0
Copyright 2011-2024 Tucsen Photonics Co., Ltd.
All rights reserved.
Technical Support Email: service@tucsen.com
TEL: +86-591-28055080-818
Fax: +86-591-28055080-826

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Summary of Contents for Tucsen Dhyana 401D

  • Page 1 Dhyana 401D User Manual V1.0.0 Copyright 2011-2024 Tucsen Photonics Co., Ltd. All rights reserved. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 2: Table Of Contents

    3.15.2. Software Trigger Mode .......................... 24 3.15.3. Hardware Trigger Mode ......................... 24 3.16. Trigger Output ............................... 29 3.16.1. Hardware Trigger output circuit ......................29 3.16.2. Trigger Output Timing Diagram ......................29 4. Installation ...............................31 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 3 8. After-sales Support ............................43 Appendix 1 : Dimensions ............................44 Appendix 2 : Specifications ...........................45 Appendix 3 : Third party software applications ....................47 Appendix 4 : Third party certification ........................48 Appendix 5 : Update log ............................50 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 4: Introduction

    In any case, TUCSEN shall not be liable for any losses or damages arising from or related to the unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or other reasons, whether caused by infringement or other reasons.
  • Page 5 3) This document may contain technical or printing errors. Under no circumstances shall TUCSEN be liable for any loss or damage caused by unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or otherwise.
  • Page 6: Safety And Warning Information

     Do not use solvents or diluents to clean the surface of the equipment, as this will damage the surface of the casing. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 7  If the voltage applied to the camera is greater than or less than the nominal voltage of the camera, the camera may be damaged or malfunction. Caution  Please refer to the specification table for the nominal voltage of the camera. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 8: Product Specifications

    Specification Quantity Image sCMOS scientific grade camera Dhyana 401D USB3.0 data cable USB flash drive Software and Drivers Optional Item Name Specification Quantity Image Hirose trigger cable HR10A-7R-6PB(73) Level conversion box Standard Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 9: Quantum Efficiency Curve

    2.2. Quantum efficiency curve Figure 2-1 Quantum efficiency curve of Dhyana 401D 2.3. Window curve Figure 2-2 Transmission curve of a standard glass window Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 10: Camera Power And Signal Connection

    If the software has been shut down but the camera light remains orange, it indicates that the camera has not been fully released and needs to be completely exited from the camera thread or be restarted before the indicator light will return to red. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 11: Features And Functions

    3. Features and Functions 3.1. Camera Introduction The Dhyana 401D camera features a compact design that is well-suited for integration into instrument systems, achieving efficient coordination between hardware and software. With a readout noise as low as 2 electrons, it ensures image signal-to-noise and accuracy. The pixel size is perfectly matched with high numerical aperture microscope objectives, making it an ideal choice for both scientific research and industrial inspection.
  • Page 12: Shutter Mode

    3.3. Shutter Mode The 401D camera uses a rolling shutter readout mode. In this mode, the camera reads out rows sequentially, with consistent exposure time for each row, but different starting exposure time Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 13: Readout Noise

    Accurate measurement of readout noise is usually achieved by capturing multiple dark field images under no light and the shortest exposure conditions, then calculating the temporal standard deviation for each pixel. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 14: Defective Pixel Correction (Dpc)

    It is not recommended to use the DPC function for these applications or to use only the weakest correction level. The Dhyana 401D adopts dynamic defective pixel correction, correcting using a 3x3 matrix of pixels. Currently, four correction levels are available, each corresponding to different thresholds, thereby controlling the intensity of defective pixel correction.
  • Page 15: Photo Response Non-Uniformity (Prnu)

    In dark field or low-light conditions, bias variations can be measured by the DSNU mentioned in Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 16: Gain Mode

    Selecting the appropriate mode based on the actual scene is essential to achieve high-quality imaging results. Table 3-1 Typical gain mode parameter table* Mode System gain (DN/e-) 2.15 0.08 Full-Well Capacity (e-) 40000 1700 43000 2.2(Median) 2.0(Median) 21(Median) Readout noise(e-) 2.7(RMS) 2.8(RMS) 22(RMS) Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 17: High Dynamic Range

    The High Gain (HG) mode has lower readout noise and is suitable for imaging scenarios with weaker signals. 3.8.3. Low Gain The Low Gain (LG) mode has a higher full well capacity, making it suitable for imaging scenarios with strong signals. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 18: Region Of Interest Readout

    9363 Note: 1) The minimum supported ROI for Dhyana 401D on Mosaic V3 is 48 (columns) × 8 (rows). 2) Frame rates are affected by computer system configuration, and it is recommended to use a computer with an i5 processor or above and a 64-bit system.
  • Page 19: Binning Readout

    4 to obtain the average grayscale value. The software Binning seclection on Mosaic V3 is shown below: Figure 3-9 The FPGA Binning seclection on Mosaic V3 is shown below: Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 20: Timestamp

    .csv format files. Figure 3-11 Timestamp export schematic Note: Applications requiring timestamp functionality generally have high time precision requirements, and it is recommended to use the To RAM image storage mode. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 21: Frame Rate Calculation

    Table 3-4 Frame rate calculation for 12-bit and 8-bit bit depth Gain mode Line time Calculation Horizontal Vertiacal Frame formula (Hn) (Vn) rate (fps) line 2048 45.68 1024 91.26 182.17 HG/LG(12Bit) 10.68μs 1/((Vn+2)*T 2048 line HDR/HG/LG(8bit) 362.92 720.28 1418.7 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 22: Frame Rate Adjustment

    45 fps adds 3.1 ms of blanking time during image output. Similarly, if the exposure time > readout time, considering an exposure time of 100 ms, setting the frame rate to 5 fps adds 100 ms of blanking time during image output. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 23: Incident Photon Calculation

    After successfully installing the Mosaic V3 software and drivers, the hardware trigger mode defaults to "internal" (live mode), the user can click Preview/Stop to control the opening and closing of the camera live mode, and click Capture to obtain the image. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 24 Figure 3-14 In the acquisition module, users can set the save path, file name, total number of frames and other information, and the image can be taken after the setting is completed. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 25: Software Trigger Mode

    It includes two modes: Standard Overlap, Standard Non-Overlap. The Mosaic V3 software provides configurations such as mode, frames/signal, exposure, edge, exposure delay, etc., to control the number of triggered images captured continuously according Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 26 Figure 3-18 Hardware trigger input circuit Note: 1) The external trigger circuit of the Dhyana 401D uses an optocoupler isolation circuit and requires an external pull-up resistor to enable the trigger function, as shown in the diagram: 3.3V voltage and 1kΩ resistor;...
  • Page 27 Figure 3-20 Trigger Delay Diagram : exposure time; T : hardware circuit delay; T : trigger jitter; T : total delay time; T : trigger filter logic idelay filter delay time;1H: one T line Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 28 Figure 3-22 Standard (Non-Overlap) Trigger timing Both of these modes support both level and edge triggering. In Standard (Non-Overlap) Trigger Mode, for example, in level trigger mode(i.e., the exposure type is selected as Width in the Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 29 Figure 3-24 Edge Trigger timing diagram 401D camera supports single-trigger multi-frame acquisition function, you can modify the number of triggering out of the picture by setting the frame/signal in the standard triggering Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 30: Trigger Output

    The camera has three external trigger output interfaces, each independently capable of outputting six timing signals. Each output signal can be independently configured on the three output ports and can simultaneously output to different devices. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 31  Global: output from the beginning of the last line of exposure to the end of the first line of exposure end (valid when the exposure time is larger than the readout time). Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 32: Installation

    Memory 8GB and above 4.2. Camera installation Figure 4-1 Dhyana 401D camera connection diagram ① Dhyana 401D camera ② USB 3.0 data cable Connect one end of the USB 3.0 data cable to the PC, ensuring that the USB is plugged into the USB 3.0 port on the back of the host.
  • Page 33: Driver Installation And Uninstallation

    (4) Selecting the contents of the installation, by default check the box to install Microsoft runtime libraries vcredist_2008 and vcredist_2013, unchecking the box may result in the software or third- party plug-ins not working; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 34 Figure 4-3 (5) Waiting for the driver installation to be completed; Figure 4-4 (6) Click "Finish" to complete the driver installation; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 35: Software Installation And Uninstallation

    If a yellow symbol appears, it indicates that the driver needs to be reinstalled. Figure 4-6 Device Manager Display 4.4. Software installation and uninstallation 4.4.1. Installation Operation steps: (1) Open the supporting USB drive, double-click to run Mosaic V3 software; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 36 (3) Select the installation content. By default, select the option to install drivers and Microsoft runtime library vc-redist_2015, canceling the installation can result in the camera not being recognized by the software; Figure 4-8 (4) Configure installation parameters and select whether to generate desktop shortcuts; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 37 Figure 4-9 (5) After confirming all installation parameters, click "Install" to start executing the installation action; Figure 4-10 (6) Waiting for installation to complete; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 38: Uninstallation

    C drive path will take effect; Figure 4-12 Uninstalling through installation package (2) Under the installation path, find unins000.exe to uninstall, double-click uninstall; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 39 Figure 4-13 (3) Uninstall from the computer program management interface; Note: After uninstalling and reinstalling the software, all software configuration information and calibration table data will be deleted. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 40: Maintenance

    1) If you cannot guarantee to complete the cleaning steps independently or do not have the required items, please be sure to contact us; 2) If the dirt still exists after following the above steps, try wiping again by following the above Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 41 If this does not solve the problem, consider that the chip is internally dirty. Please be sure to contact us at this time. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 42: Troubleshooting

    3.0 interface is used, the frame rate may not reach the nominal frame rate. 4) Even if you are using a USB 3.0 interface, using a HUB, extension, or other adapters can also prevent achieving the nominal frame rate. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 43: Faqs

    If it is an ambient light source, switching off the illumination source is sufficient. If from an irradiated sample light source, a regulated light source is required for illumination. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 44: After-Sales Support

    3) Contact professionals for technical support:  TEL: +86-591-28055080-818  Email: service@tucsen.com  Visiting the official website to leave a message: http://www.tucsen.com 4) Please prepare the following information in advance:  Camera model and S/N (product serial number);  Software version number and computer system information;...
  • Page 45: Appendix 1 : Dimensions

    Appendix 1 : Dimensions Unit: mm, Diameter: ø. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 46: Appendix 2 : Specifications

    Hardware , Software Output Trigger Signals Exposure start, Global, Readout end, High level, Low level , Trigger ready Trigger Interface Hirose-6-pin Data Interface USB 3.0 Bit Depth 8 bit, 12 bit, 16 bit Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 47 Mosaic V3, SamplePro, LabVIEW, MATLAB, Micro-Manager2.0 C, C++, C#, Python Operating System Windows, Linux Operating Temperature 0~40℃, Humidity 10~85% Environment Note: The parameters in this table are typical values and are subject to change without notice. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 48: Appendix 3 : Third Party Software Applications

    Appendix 3 : Third party software applications Provide plugins for calling third-party software (LabVIEW, Matlab, Micro-Manager2.0, etc.), please click on the link to download the configuration: Tucsen camera software download - Tucsen Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 49: Appendix 4 : Third Party Certification

    Appendix 4 : Third party certification Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 50 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 51: Appendix 5 : Update Log

    Appendix 5 : Update log Version Date Modified Content V1.0.0 2024-8-14 Create this document. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

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