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Tucsen Dhyana 400BSI V3 User Manual

Scmos scientific grade camera
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Dhyana 400BSI V3
User Manual
V1.0.1
Copyright 2011-2024 Tucsen Photonics Co., Ltd.
All rights reserved.
Technical Support Email: service@tucsen.com
TEL: +86-591-28055080-818
Fax: +86-591-28055080-826

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  • Page 1 Dhyana 400BSI V3 User Manual V1.0.1 Copyright 2011-2024 Tucsen Photonics Co., Ltd. All rights reserved. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 2: Table Of Contents

    3.15. Incident Photon Calculation .......................... 27 3.16. Acquisition Mode ............................28 3.16.1. Live mode .............................. 28 3.16.2. Software Trigger Mode .......................... 29 3.16.3. Hardware Trigger Mode ......................... 29 3.16.4. Rolling Shutter Control Mode ........................ 36 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 3 7.1. Why is the brightness of the captured image inconsistent with the preview window? ........59 7.2. Stripe like flicker appears in the camera preview image ................. 59 8. After-sales Support ............................60 Appendix 1 : Dimensions ............................61 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 4 Appendix 2 : Specifications ...........................62 Appendix 3 : Third party software applications ....................64 Appendix 4 : Third party certification ........................65 Appendix 5 : Dew point table ..........................67 Appendix 6 : Update log ............................68 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 5: Introduction

    In any case, TUCSEN shall not be liable for any losses or damages arising from or related to the unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or other reasons, whether caused by infringement or other reasons.
  • Page 6 3) This document may contain technical or printing errors. Under no circumstances shall TUCSEN be liable for any loss or damage caused by unauthorized use of the content of this document, whether direct, indirect, special, incidental, consequential or otherwise.

  • Page 7: Safety And Warning Information

    ⚫ Please ensure that there is at least 10 cm of space around the device ventilation opening to ensure airflow flow. Do not block the ventilation openings of the equipment during use, otherwise it may cause internal temperature to be too high and damage the equipment. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 8 ⚫ If the voltage applied to the camera is greater than or less than the nominal voltage of the camera, the camera may be damaged or malfunction. Caution ⚫ Please refer to the specification table for the nominal voltage of the camera. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 9: Product Specifications

    2. Product specifications 2.1. Packaging List Standard Item Name Specification Quantity Image sCMOS scientific grade camera Dhyana 400BSI V3 Power adapter 12V/8A Power adapter power cord Three core 250V/2.5A USB3.0 data cable USB flash drive Software and Drivers Water pipes...

  • Page 10: Quantum Efficiency Curve

    Optional Item Name Specification Quantity Image CameraLink frame grabber Firebird Frame grabber cable Level conversion box Standard 2.2. Quantum efficiency curve Figure 2-1 Quantum efficiency curve of Dhyana 400BSI V3 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 11: Window Curve

    2.3. Window curve Figure 2-2 Transmission curve of a standard glass window 2.4. Quartz window curve Figure 2-3 Transmission curve of a quartz window Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 12: Camera Power And Signal Connection

    If the software has been shut down but the camera light remains orange, it indicates that the camera has not been fully released and needs to be completely exited from the camera thread or be restarted before the indicator light will return to red. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 13: Features And Functions

    3. Features and Functions 3.1. Camera Introduction The Dhyana 400BSI V3 is a new generation sCMOS camera with a 6.5-micron pixel size that perfectly matches high numerical aperture microscopes, balancing the demands for high spatial sampling rates and high sensitivity, adaptable to various application scenarios. This camera supports USB and CameraLink interface.

  • Page 14: Shutter Mode

    USB, Ethernet, etc. 3.3. Shutter Mode The 400BSI V3 camera uses a rolling shutter readout mode. In this mode, the camera reads out Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 15: Front-Illuminated And Back-Illuminated Scmos Technology

    The 400BSI V3 camera adopts a back-illuminated chip, achieving a peak QE of around 95%. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 16: Readout Noise

    Since there are usually a few pixels with exceptionally high readout noise on the sCMOS camera chip—having less impact on the median than on the RMS—median values are generally lower than RMS values. Figure 3-5 Readout noise distribution of an typical sCMOS camera Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 17: Defective Pixel Correction (Dpc)

    It is not recommended to use the DPC function for these applications or to use only the weakest correction level. The Dhyana 400BSI V3 adopts dynamic defective pixel correction, correcting using a 3x3 matrix of pixels. Currently, four correction levels are available, each corresponding to different thresholds, thereby controlling the intensity of defective pixel correction.

  • Page 18: Photo Response Non-Uniformity (Prnu)

    Section 3.7. In bright environments, the influence of gain also needs to be considered. Differences in gain and offset changes are measured by Photo Response Non- Uniformity (PRNU), which is the ratio of detected electrons to displayed DN. Given that the Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 19: Gain Mode

    Table 3-1 Typical gain mode parameter table* High Sensitivity Mode High Speed（11bit） （16bit） （12bit） System gain (DN/e-) Full-Well Capacity (e-) 45000 1500 2000 1.6(Median) 1.1(Median) 1.9(Median) Readout noise(e-) 1.7(RMS) 1.2(RMS) 2.0(RMS) Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 20: High Dynamic Range

    CMS mode image. This mode is configured with higher gain and is suitable for applications with weak signals. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 21: High Speed

    It first resets the entire pixel array of the chip simultaneously, then exposes all rows simultaneously. After exposure, data is read out row by row, with the exposure time increasing row by row. In ideal applications, the light source should be turned off immediately after the Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 22: Region Of Interest Readout

    4, and the column window must be a multiple of 8. Typical ROI frame rates for different interfaces of the camera can be found in Table 3-2, Table 3- Table 3-2 Typical ROI area measured frame rate (fps) table for Dhyana 400BSI V3 camera under USB 3.0 Column (Pixel)

  • Page 23: Binning Readout

    2048 Note: 1) The minimum supported ROI for Dhyana 400BSI V3 on Mosaic V3 is 48 (columns) × 8 (rows). 2) Frame rates are affected by computer system configuration, and it is recommended to use a computer with an i5 processor or above and a 64-bit system.
  • Page 24 The FPGA Binning seclection on Mosaic V3 is shown below: Figure 3-14 The 2048 x 2048 (Enhance) resolution uses Tucsen's own signal enhancement algorithm, which differs from 2 x 2 Binning in that it accumulates neighboring four pixels into one output pixel, effectively improving the signal-to-noise ratio without sacrificing resolution.

  • Page 25: Timestamp

    .csv format files. Figure 3-15 Timestamp export schematic Note: Applications requiring timestamp functionality generally have high time precision requirements, and it is recommended to use the To RAM image storage mode. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 26: Frame Rate Calculation

    11.2 μs 2048 43.5 1024 86.9 173.0 Global Reset 2048 343.4 1/{(Vn+4)*T line 676.4 1313.0 7440.5 2048 100.0 1024 200.0 400.0 High Speed 7.2 μs 2048 800.0 ） 1/(Vn/2048/Y 1600.0 3200.0 25600.0 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 27 Sensitivity/High Speed modes with T > T , frame rate = 1/T (s); readout 3) The maximum bandwidth of USB3.0 is about 360MB/s, and the maximum bandwidth of CameraLink is about 800MB/s. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 28: Frame Rate Adjustment

    Where:P represents the incident number of photons.DN is the grayscale value of the light signal. K is the system gain (refer to Table 3-1) in (DN/e-). Q(λ) corresponds to the quantum efficiency at the wavelength.Offset s the camera's background value in DN. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 29: Acquisition Mode

    Capture to obtain the image. Figure 3-17 Users can set the exposure time, gain mode and other camera parameters, and preview them in real time through the preview window to get a suitable image. Figure 3-18 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 30: Software Trigger Mode

    Figure 3-20 3.16.3. Hardware Trigger Mode Hardware trigger mode waits for an external level trigger signal command to capture an image. It includes three modes: Standard Overlap, Standard Non-Overlap, and Synchronization. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 31 This function filters out spikes in the level signal. The filtering time range can be set to [0, 1000000] μs, with the default being 0. The following figure takes the filtering time set to 1 ms as an example: Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 32 , within the range of one line time. If the Trigger Filter function is used, a Trigger idelay logic Filter Delay Time T is added to this. filter Figure 3-24 Schematic of trigger delay under Rolling Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 33 Standard (Overlap) Trigger Mode: In this mode, when the camera is in live mode, it responds to the trigger signal immediately after the first row of exposure ends. Figure 3-26 Standard (Overlap) Trigger timing Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 34 Level trigger mode is not continuous shooting; it is commonly used to capture stationary or slowly moving objects. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 35 (i.e., the reciprocal of the frame rate) to ensure that a frame of image is complete and error-free. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 36 (refer to Figure 3-21), for example, by setting the number of 2, the triggering timing diagram is as follows: Figure 3-30 Single-trigger multi-frame acquisition timing diagram Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 37: Rolling Shutter Control Mode

    3.16.4. Rolling Shutter Control Mode Rolling shutter control is supported in Dhyana 400BSI V3 camera. This mode utilizes the characteristic of rolling shutter readout, allowing seamless synchronization with the movement of LightSheet imaging systems. This mode significantly improves the signal-to-noise ratio of Technical Support Email: service@tucsen.com...
  • Page 38 The rolling shutter starts at the top of the sensor and scans down to the bottom of the last line. Each subsequent frame acquisition starts with the first line at the top. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 39 The image acquired in this mode is oriented in the same direction as the downward scan direction. Figure 3-36 Schematic diagram of Down-up cycle scanning Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 40: Trigger Output

    Each output signal can be independently configured on the three output ports and can simultaneously output to different devices. Figure 3-38 Trigger output timing diagram ⚫ High: always output high level. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 41 (valid when the exposure time is larger than the readout time), the default pulse width is 5ms, which is customizable. And the output is in the TRIG.OUT2 interface by default. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 42: Cooling

    The Dhyana 400BSI V3 camera supports both air cooling and water cooling modes, allowing users to choose the appropriate cooling method based on the actual usage environment.

  • Page 43: Installation

    4.2. Camera installation Figure 4-1 Dhyana 400BSI V3 camera connection diagram ① Dhyana 400BSI V3.0 camera ② Power adapter ③ USB 3.0 data cable ④...

  • Page 44: Water Cooling Pipe Installation

    (1) Place the camera on a stable workbench; (2) Connect the water cooling pipe to the water pipe joint on the camera and make sure it is inserted in place, as shown in Figure 4-2 below; Figure 4-2 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 45: Software Fan Status

    (3) Press the adapter valve sleeve and remove the water hose from the recirculating water chiller to drain the internal water; (4) Press the water connector and remove the camera's water hose from the hose connector. First, Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 46 Warning: 1) Do not turn off the recirculating water chiller during camera operation, as this may cause the chip to run at a high temperature continuously, leading to damage; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 47: Frame Grabber Installation

    1) When installing and disassembling the Frame grabber, be sure to operate with power off; 2) When connecting the CamraLink cable, please operate with the camera power off and do not hot plug or unplug. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 48: Driver Installation And Uninstallation

    (4) Selecting the contents of the installation, by default check the box to install Microsoft runtime libraries vcredist_2008 and vcredist_2013, unchecking the box may result in the software or third- party plug-ins not working; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 49 Figure 4-8 (5) Waiting for the driver installation to be completed; Figure 4-9 (6) Click "Finish" to complete the driver installation; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 50: Cameralink Driver Installation

    CameraLink2 interfaces on the computer host through two CameraLink cables, and the interface sequence needs to be one-to-one corresponding; (2) Open the matching USB flash drive. Find the installation package for the acquisition card Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 51: Cameralink Driver Uninstallation

    Taking the Windows 11 system as an example, in the computer settings, click on Applications>Installed Applications, find Active Silicon FireBird CL, click on the three points on the right to uninstall, and follow the prompts to complete the uninstallation. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 52: Software Installation And Uninstallation

    (1) Open the supporting USB drive, double-click to run Mosaic V3 software; (2) Select the installation path, default to C drive, users can customize the installation path according to your needs; Figure 4-15 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 53 Figure 4-16 (4) Configure installation parameters and select whether to generate desktop shortcuts; Figure 4-17 (5) After confirming all installation parameters, click "Install" to start executing the installation action; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 54: Uninstallation

    There are three ways to uninstall Mosaic software: (1) By uninstalling through the installation package, the existing version on the computer will be uninstalled when the installation package runs, and the default C drive path will take effect; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 55 (2) Under the installation path, find unins000.exe to uninstall, double-click uninstall; Figure 4-21 (3) Uninstall from the computer program management interface; Note: After uninstalling and reinstalling the software, all software configuration information and calibration table data will be deleted. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 56: Maintenance

    1) Priority is given to the use of air blowing, the use of ear washing ball or air blowing off the general dust; together with the brush can remove most of the dust; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 57 2) If the dirt still exists after following the above steps, try wiping again by following the above steps. If this does not solve the problem, consider that the chip is internally dirty. Please be sure to contact us at this time. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 58: Troubleshooting

    4) If water-cooling is used, make sure that the water-cooling cycle is functioning properly. 6.4. The frame rate cannot reach the nominal level 1) Confirm whether the exposure time affects the frame rate, and set the minimum exposure time to confirm the frame rate; Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 59 6) For users who require high-speed image storage, if the frame rate cannot reach the nominal value using the CameraLink interface, it is necessary to check whether the bandwidth of the acquisition card is limited/whether the recommended acquisition card interface is used. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 60: Faqs

    If it is an ambient light source, switching off the illumination source is sufficient. If from an irradiated sample light source, a regulated light source is required for illumination. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 61: After-Sales Support

    3) Contact professionals for technical support: ⚫ TEL: +86-591-28055080-818 ⚫ Email: service@tucsen.com ⚫ Visiting the official website to leave a message: http://www.tucsen.com 4) Please prepare the following information in advance: ⚫ Camera model and S/N (product serial number); ⚫ Software version number and computer system information;...

  • Page 62: Appendix 1 : Dimensions

    Appendix 1 : Dimensions Unit: mm, Diameter: ø. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 63: Appendix 2 : Specifications

    Appendix 2 : Specifications Camera Model Dhyana 400BSI V3 Sensor Type BSI sCMOS Sensor Model Gpixel GSENSE2020BSI Peak QE 95% @ 600 nm Color / Mono Mono Array Diagonal 18.8 mm Effective Area 13.3 mm x 13.3 mm Resolution 2048 (H) x 2048 (V) Pixel Size 6.5 μm x 6.5 μm...
  • Page 64 PCIe 2x4, supports 2GB/s transfer from motherboard to computer Transmission Speed 1.7 GB/s Format Half length PCI Express Note: The parameters in this table are typical values and are subject to change without notice. Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 65: Appendix 3 : Third Party Software Applications

    Appendix 3 : Third party software applications Provide plugins for calling third-party software (LabVIEW, Matlab, Micro-Manager, etc.), please Tucsen camera software download - Tucsen click on the link to download the configuration: Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 66: Appendix 4 : Third Party Certification

    Appendix 4 : Third party certification Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...
  • Page 67 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 68: Appendix 5 : Dew Point Table

    20.3 22.3 10.5 13.9 16.7 19.1 21.3 23.2 11.4 14.8 17.6 20.1 22.3 24.2 12.3 15.7 18.6 21.1 23.3 25.2 13.2 16.6 19.5 22.0 24.2 26.2 14.0 17.5 20.4 23.0 25.2 27.2 Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

  • Page 69: Appendix 6 : Update Log

    Appendix 6 : Update log Version Date Modified Content V1.0.0 2023-9-28 Create this document. V1.0.1 2024-2-26 Adjustments have been made based on the new firmware version of the camera Technical Support Email: service@tucsen.com TEL: +86-591-28055080-818 Fax: +86-591-28055080-826...

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