YMC HT Original Manual
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YMC HT Original Manual

High temperature chromatography column
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YMC HT
High temperature chromatography column
In order to avoid personal injuries and damage to equipment, please read this manual carefully
and act on the recommendations given. Keep this manual accessible for the user at all times.
27.05.2024
Version 1.1

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  • Page 1 Original- Manual YMC HT High temperature chromatography column In order to avoid personal injuries and damage to equipment, please read this manual carefully and act on the recommendations given. Keep this manual accessible for the user at all times. 27.05.2024...
  • Page 2 YMC Europe GmbH YMC Europe GmbH Schöttmannshof 19 46539 Dinslaken, Germany +49 02064 427-0 info@ymc.eu www.ymc.eu YMC HT High temperature chromatography column 2 / 43...

  • Page 3: Table Of Contents

    YMC Europe GmbH Information on manual Contents Information on manual ................5 General....................5 Explanation of warnings ................ 5 Structure of instructions ................ 6 Guidelines ..................... 6 Figures ....................6 Safety ......................7 Intended use ..................7 General safety instructions ..............7 Personnel requirements ................
  • Page 4 YMC Europe GmbH Information on manual Storage ....................39 Transport ..................... 39 Disposal ....................40 Appendix ....................41 11.1 Spare parts glass body ............... 41 11.2 Spare parts end pieces ............... 42 11.3 Spare parts coupling unit ..............42 11.4 Index....................

  • Page 5: Information On Manual

    YMC Europe GmbH Information on manual Information on manual General This manual includes important messages and information for the intended use of this glass column. The manual must be kept accessible for the user at all times. In order to avoid injuries and damages, this manual should be read carefully prior to use and the recommendations given should be acted upon.

  • Page 6: Structure Of Instructions

    YMC Europe GmbH Information on manual Structure of instructions The instructions relate directly to an activity. They are structured in a taks oriented manner. Always perform the individual steps in the order in which they are given. Instructions are structure as follows and are marked by the corresponding levels: ...

  • Page 7: Safety

    YMC Europe GmbH Safety Safety This section gives an overview of all the important safety aspects for the protection of personnel and for the safe and trouble-free handling of the glass column. Other task-specific safety requirements are contained in the sections relating to the respective operation.

  • Page 8: Personal Protective Equipment

    YMC Europe GmbH Safety Personal protective equipment In order to reduce the risks during working on the column, always use the recommended personal protective equipment. The following protective equipment is required: Symbol Phase of life Action • Column packing Protective gloves Operation •...

  • Page 9: Specifications And Overview

    Specifications and overview Fields of application The YMC HT glass column is especially designed for high temperature applications. The column is compatible with common BioLC separation modes (IEX, SEC, Affinity, IMAC, HIC, MMC) as well as NP and RP mode in case the chemical resistance is given.
  • Page 10 YMC Europe GmbH Specifications and overview PED* Fluidgroup: Group 1 referred to EU Guideline 2014/68/EU PED* safety requirement: Article 4.3 of EU Guideline 2014/68/EU Glass body: Borosilicate glass 3.3 Frits: Glass Seals: FFKM ¼‘‘-28G thread Connection: FEP tubing 10-15 mm ID: 0.8 x 1.6 mm 25-50 mm ID: 1.6 x 3.2 mm...

  • Page 11: Chemical Resistance

    These do not exempt the users of our products from their own testing. If desired solvent is not listed, YMC will be pleased to advise you. ATTENTION When using solvent mixtures, the resistance may differ significantly from those of the pure solvent.

  • Page 12: Overview

    All parts listed for the column body and the end pieces are in the appendix. The YMC HT column consists of a glass body equipped with a tempering jacket and two variable end pieces. The end pieces can be removed from the column body.

  • Page 13: Variable End Piece

    YMC Europe GmbH Spezifikationen und Übersicht 3.4.1 Variable end piece 1 Frit 6 Spring washer 2 O-ring 7 Snap ring 3 Outer spindle 8 Counter nut 4 Inner spindle 9 Screw nut 5 Counter screw The variable end piece consists of the counter screw connection and the piston. The piston consists of the inner (4) and outer spindle (3).

  • Page 14: Delivery

    Delivery Delivery The YMC HT glass columns are shipped as pre-assembled units. A quicklguide, acces- sory bag and Certificate of Quality Control is delivered with every glass columns. Using the serial number, the column can be identified clearly. The product code includes in- formation of inner diameter, length and frit porosity.

  • Page 15: Accessory Bag

    Delivery Accessory bag YMC HT glass columns are shipped with an accessory bag containing different fittings for connecting the column to the system. The tubing is connected to the piston outlet using a ferrule and a nut. For connecting the tubing to the system, 10-32 or M6 fittings are included depending on the column inner diameter.
  • Page 16 YMC Europe GmbH Delivery Accessory bag 10-15 mm ID Part number Description Quantity 1/16‘‘ FEP tubing (0.8 x 1.6 mm) PT0,8FE1,6 Nut, 1/4‘‘-28G for 1/16‘‘ tubing JR-55050 Ferrule, callapsible, for 1/16‘‘ tubing JR-CFL-CB1PK-S 10-32 nut (one-piece-fitting) for 1/16‘‘ tubing JR-58000 M6 nut for 1/16‘‘...

  • Page 17: Requirements For Operation

    YMC Europe GmbH Requirements for operation Requirements for operation Safety instructions for use WARNING Use correctly matched protective clothing when working with the glass column. WARNING Do not put the glass column under gas pressure. CAREFUL Operate the column only with pressures within the stated specifications.

  • Page 18: Glass Column Set Up

    YMC Europe GmbH Requirements for operation Glass column set up For columns with 25 mm and 50 mm inner diameter, column stands are availabe to set up the column.  The glass column is set up as follows: 1. Make sure that the glass column is fixed in the stand / holder.
  • Page 19 YMC Europe GmbH Requirements for operation Standard procedure pressure test  The pressure test should be performed as follows: 1. Assemble the system according to the schematic overview of a pressure test. The 3-way valve must not be set to the back pressure regulator.

  • Page 20: Operation

    YMC Europe GmbH Operation Operation WARNING Risk of explosion Severe injuries possible due to glass splinters. ► Never put the glass column under gas pressure! In order to guarantee safe operation, the glass column must be used for the intended use only ( Chapter 2.1, p.

  • Page 21: Piston Adjustment

    YMC Europe GmbH Operation Piston adjustment  The piston is adjusted as follows: 1. O-ring seals by turning the screw nut clockwise. ✓ O-ring is sealed when a constant black line appears in the glass body. 2. Hold the closed counter screw connection.

  • Page 22: Glass Column Packing

    YMC Europe GmbH Operation Glass column packing CAREFUL Packing material Eye and skin injuries possible trough contact with packing material. ► Wear the prescribed personal protective equipment. ATTENTION Adapt the packing method to the manufacturer´s packing material information. Improper packing conditions can cause damage or completely destroy the material.

  • Page 23: Slurry Preparation

    YMC Europe GmbH Operation 6.5.1 Slurry preparation CAREFUL Formation of dust with dry packing material Lung irritation possible if packing material is inhaled. ► Read the material safety data sheet from the packing material manufacturer. ► Wear a dust mask.

  • Page 24: Slurry Column Packing Under Flow

    YMC Europe GmbH Operation 6.5.2 Slurry column packing under flow  The column is packed with the slurry method under flow as follows: 1. Flush the system for packing with the packing solvent. 2. Check the column parts with regards to cleanness and function.
  • Page 25 YMC Europe GmbH Operation 23. Remove the air from the tubing. 24. Connect the tubing to the upper piston. 25. Remove the plug at the lower piston. 26. Connect the tubing to the lower piston. 27. Increase the flow rate stepwise until you reach the required flow rate and packing pressure.

  • Page 26: Slurry Column Packing With Sedimentation

    YMC Europe GmbH Operation 50. Lower the upper piston until you reach the column bed. ✓ Take care that the upper piston is completely filled with liquid and solvent comes out. 51. Remove the air from the tubing of the system.
  • Page 27 ✓ Only in this way can optimum sealing of the O-ring be guaranteed. 18. Do not move the glass column until packing is completed. ✓ YMC recommends covering the open column body during sedimentation with some plastic film. 19. Wait until the packing material has settled (sedimentation).

  • Page 28: Column Test And Release Criteria

    The column test and the release criteria are selected with regards to the later application requirements. The preparation of the required specifications for the final use of a column or for the purification of a target molecule is determined individually. YMC will gladly support you.

  • Page 29: Packing Adapter

    YMC Europe GmbH Operation Packing adapter Due to the increased maximum column volume, the slurry concentration can be reduced. This way, the packing process is simplified. The packing adapter consists of a coupling unit and an additional glass body.  The packing adapter is assembled as follows: 1.

  • Page 30: Glass Column Unpacking

    YMC Europe GmbH Operation Glass column unpacking WARNING Glass column under high pressure Eye or skin injuries possible due to remaining pressure in the tubing or in the glass column. ► Stop the pump during working on the glass column.

  • Page 31: Cleaning And Maintenance

    YMC Europe GmbH Cleaning and maintenance Cleaning and maintenance WARNING Incorrect maintenance Severe injuries when used after incorrect maintenance are possible, if the column or the column parts do not withstand the operating pressure. ► Only authorised and qualified personnel should carry out the maintenance.

  • Page 32: Notes For Cleaning-In-Place (Cip)

    YMC Europe GmbH Cleaning and maintenance Notes for Cleaning-In-Place (CIP) CAREFUL Packing material Eye and skin injuries possible through contact with packing material. ► Wear the prescribed personal protective equipment. ATTENTION Cleaning with inappropriate solvents Material damage possible due to corrosion, leaching or deformation.

  • Page 33: Cleaning And Maintenance Plan

    YMC Europe GmbH Cleaning and maintenance Cleaning methods for CIP Treatment Type of contamination Sterilisation Depyrogenation Proteolysis of adsorbed Pepsin, pH 1.5 - 2 proteins Non-ionic detergents Removal of hydrophobic (Triton X-100, Tween proteins and lipids Cationic detergents Removal of hydrophobic...

  • Page 34: Cleaning

    YMC Europe GmbH Cleaning and maintenance Cleaning WARNING Glass column under high pressure Eye or skin injuries possible due to the remaining pressure in the tubing or in the glass column. ► Stop the pump while working on the glass column.

  • Page 35: Replacement Of Spare Parts

    YMC Europe GmbH Cleaning and maintenance Replacement of spare parts 7.4.1 Frit replacement ATTENTION Faulty assembly Damage to the pistons possible due to faulty assembly. ► Make sure that the frit fits into the opening of the inner spindle exactly. Otherwise, the pistons or the frits may be damaged.

  • Page 36: O-Ring Replacement

    YMC Europe GmbH Cleaning and maintenance 7.4.2 O-ring replacement ATTENTION Screw nut loosened completely Damage to the inner spindle possible when the screw nut is loosened completely. ► Make sure that the inner spindle cannot slip free from the outer spindle when the screw nut is loosened completely.

  • Page 37: Troubleshooting

    YMC Europe GmbH Troubleshooting Troubleshooting WARNING Incorrect maintenance Severe injuries during operation after incorrect maintenance possible if the column or column parts do not withstand the operating pressure. ► Only authorised and qualified personnel should carry out the maintenance. WARNING Incorrect replacement of spare parts Severe injuries due to breakage possible, if authorised spare parts are not used.
  • Page 38 Remove the O-ring and move it by O-ring not in the right hand. Assemble the O-ring again. position If faults occur which are not listed here, please contact YMC for support. YMC HT High temperature chromatography column 38 / 43...

  • Page 39: Storage, Packaging And Transport

    YMC Europe GmbH Storage, packaging and transport Storage, packaging and transport WARNING Solvents in the glass column Dangers possible due to liquids leaking from the column during transportation. ► Unpack and clean the column before transport. Storage For storage of packed columns, the chemical resistance of the wetted parts and of the packed stationary phase (please contact the manufacturer) should be considered.

  • Page 40: Disposal

    YMC Europe GmbH Disposal Disposal For the disposal of the glass column it has to be disassembled and disposed according to the national and other applicable environmental regulations. YMC HT High temperature chromatography column 40 / 43...

  • Page 41: Appendix

    YMC Europe GmbH Appendix Appendix 11.1 Spare parts glass body Pos. Max. bed length Part No. Description Material [mm] [mm] HT10/120/K Glass body Borosilicate glass 3.3 HT10/200/K Glass body Borosilicate glass 3.3 HT10/300/K Glass body Borosilicate glass 3.3 HT10/450/K Glass body Borosilicate glass 3.3...

  • Page 42: Spare Parts End Pieces

    YMC Europe GmbH Appendix 11.2 Spare parts end pieces Pos. ID [mm] Part No. Description Material O-rings ECO1011K/P2 FFKM Pack of 2 O-rings ECO1511K/P2 FFKM Pack of 2 O-rings ECO2511K/P2 FFKM Pack of 2 O-rings ECO5011K/P2 FFKM Pack of 2 Frits, 10-16 µm...

  • Page 43: Index

    YMC Europe GmbH Appendix 11.4 Index Cleaning Cleaning-In-Place Delivery contents Disposal Frit replacement Glass body replacement Packaging Packing Packing adapter Personal protective equipment Pressure test Protective equipment Resistance, chemical Signal word Signal word field Slurry Slurry preparation Slurry method sedimentation...

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