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Summary of Contents for YMC Pilot
- Page 1 Original- Manual YMC Pilot Low pressure chromatography column In order to avoid personal injuries and damage to equipment, please read this manual carefully and act on the recommendations given. Keep this manual accessible for the user at all times. 03.05.2021...
- Page 2 YMC Europe GmbH YMC Europe GmbH Schöttmannshof 19 46539 Dinslaken, Deutschland +49 02064 427-0 Info@ymc.de www.ymc.de YMC Pilot Low pressure chromatography column 2 / 49...
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Page 3: Table Of Contents
YMC Europe GmbH Contents Contents Information on manual ................5 General....................5 Explanation of warnings ................ 5 Structure of instructions ................ 6 Guidelines ..................... 6 Figures ....................6 Safety ......................7 Intended use ..................7 General safety instructions ..............7 Personnel requirements ................ - Page 4 YMC Europe GmbH Information on manual Replacement of spare parts ..............40 7.4.1 Frit replacement ................40 7.4.2 O-ring plunger replacement ............40 7.4.3 Replacing the glass body ............... 41 Troubleshooting ..................42 Storage, packaging and transport ............44 Storage ....................44 Transport .....................
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Page 5: Information On Manual
YMC Europe GmbH Information on manual Information on manual General This manual includes important messages and information for the intended use of this glass column. The manual must be kept accessible for the user at all times. In order to avoid injuries and damages, this manual should be read carefully prior to use and the recommendations given should be acted upon. -
Page 6: Structure Of Instructions
YMC Europe GmbH Information on manual Structure of instructions The instructions relate directly to an activity. They are structured in a task oriented manner. Always perform the individual steps in the order in which they are given. Instructions are structured as follows and are marked by the corresponding levels: ... -
Page 7: Safety
YMC Europe GmbH Safety Safety This section gives an overview of all the important safety aspects for the protection of personnel and for the safe and trouble-free handling of the glass column. Other task-specific safety requirements are contained in the sections relating to the respective operation. -
Page 8: Personal Protective Equipment
YMC Europe GmbH Safety Personal protective equipment In order to reduce the risks during working on the column, always use the recommended personal protective equipment. The following protective equipment is required: Symbol Phase of life Action • Column packing Protective gloves Operation •... -
Page 9: Specifications And Overview
Specifications and overview Fields of application The YMC Pilot glass column is especially designed for pilot scale use. The column is compatible with common BioLC separation modes (IEX, SEC, Affinity, IMAC, HIC, MMC) as well as NP and RP mode in case the chemical resistance is given. -
Page 10: Specifications - Ab Version
YMC Europe GmbH Specifications and overview Specifications – AB version 3.2.1 Length glass body [mm] Inner diameter [mm] Bed length [mm] 70-420 70-410 90-430 20-390 420-770 420-760 440-780 330-740 2.9 – 1.4 – 6.5 – 13.9 – 22.8 – Column volume 0.6 -... -
Page 11: Chemical Resistance
These do not exempt the users of our products from their own testing. If desired solvent is not listed, YMC will be pleased to advise you. ATTENTION When using solvent mixtures, the resistance may differ significantly from those of the pure solvent. -
Page 12: Chemical Resistance - Ab Version
0.5 M Na Good Good Good Good Good Good Good YMC recommends avoiding the following chemicals: • Strong oxidizing solvents • Halogenated compounds • Chlorinated solvents • Aromatic compounds (e.g. toluene) • High concentrations of strong acids • THF YMC Pilot... -
Page 13: Chemical Resistance - Sr Version
Good Good 0.5 M Na Good Good Limited Good YMC recommends avoiding the following chemicals: • Strong oxidizing solvents • Halogenated compounds • Chlorinated solvents • High concentrations of strong acids YMC Pilot Low pressure chromatography column 13 / 49... -
Page 14: Overview
YMC Europe GmbH Specifications and overview Overview All parts listed for the column body and the end pieces are in the appendix. The glass column consists of the adjustable end piece (1), the column body (2), the fixed end piece (3) and the column stand. The column stand is fixed to the column body. -
Page 15: Adjustable End Piece
YMC Europe GmbH Specifications and overview 3.4.1 Adjustable end piece The adjustable end piece consists of a closing disc and the piston. The piston consists of the pressure piece, the internal and external spindle, the plunger, the frit and the O- ring. - Page 16 YMC Europe GmbH Specifications and overview 3.4.1.1 Adjustable end piece 100 mm, 140 mm, 200 mm ID 1 Tubing 15 O-ring plunger 2 Connection nut inner spindle 16 Countersunk screw 3 Set screw 17 Frit 4 Tension nut 18 O-ring frit...
- Page 17 YMC Europe GmbH Specifications and overview 3.4.1.2 Adjustable end piece 300 mm ID 1 Frit 18 Internal spindle (AEP) 2 Plunger 19 Tubing 3 Spring 20 External spindle (AEP) 4 Threaded insert 21 Hub 5 Cylinder screw 22 Sliding disc small...
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Page 18: Piston Front
YMC Europe GmbH Specifications and overview 3.4.2 Piston front 1 Pressure piece 5 Distributor plate 2 O-ring plunger 6 Frit 3 Plunger 7 Countersunk screw 4 O-ring frit The piston front design is the same for the adjustable and the fixed end pieces. The piston front consists of the plunger (3) and the pressure piece (1) which is located on springs on the plunger. -
Page 19: Fixed End Piece
YMC Europe GmbH Specifications and overview 3.4.3 Fixed end piece The fixed end piece consists of a closing disc and the piston. The piston consists of the pressure piece, the internal and external spindle, the plunger, the frit and the O- ring. - Page 20 YMC Europe GmbH Specifications and overview 3.4.3.2 Fixed end piece 300 mm ID 1 Handle bar 13 Frit 2 Closing disc (FEP) 14 Cylinder screw 3 Tension nut 15 Threaded insert 4 Connection nut inner spindle 16 Coupler plunger 5 Set screw...
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Page 21: Column Body
YMC Europe GmbH Specifications and overview 3.4.4 Column body 1 Column disc (AEP) 4 Column disc (FEP) 2 Rod 5 Main pillar 3 Glass body 6 Castor The glass body is fixed between the column disc (AEP) and the column disc (FEP) by the rods. -
Page 22: Delivery
Delivery Delivery The YMC Pilot glass columns are shipped as pre-assembled units. A documentation folder is delivered with every glass column. Based on the label on the column it is always possible to determine the column version. Using the serial number, the column can be identified clearly. -
Page 23: Requirements For Operation
YMC Europe GmbH Requirements for operation Requirements for operation Safety instructions for use WARNING Use correctly matched protective clothing when working with the glass column. WARNING Do not put the glass column under gas pressure. CAREFUL Operate the column only with pressures within the stated specifications. -
Page 24: Glass Column Set Up
YMC Europe GmbH Requirements for operation Glass column set up Devices required: • Collection vessel The glass column is set up as follows: 1. Make sure that the glass column is standing on fixed ground. 2. Make sure that the glass column is standing vertical in the final operating place. - Page 25 YMC Europe GmbH Requirements for operation Standard procedure pressure test 1 Glass column 3 Gate valve 2 Gate valve 4 Pressure gauge The pressure test should be performed as follows: 1. Assemble the system according to the schematic overview of a pressure test.
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Page 26: Operation
YMC Europe GmbH Operation Operation WARNING Risk of explosion Severe injuries possible due to glass splinters. ► Never put the glass column under gas pressure! In order to guarantee safe operation, the glass column must be used for the intended use only ( Chapter 2.1, p. -
Page 27: Removal Of End Pieces
YMC Europe GmbH Operation Removal of end pieces Devices required: • Clean and soft surface • hex-wrench WARNING Weight of the adjustable and fixed end piece Severe injuries and damage of the glass body possible. ► Wear the prescribed personal protective equipment. -
Page 28: Glass Column Packing
YMC Europe GmbH Operation Glass column packing CAREFUL Packing material Eye and skin injuries possible through contact with packing material. ► Wear the prescribed personal protective equipment. ATTENTION Adapt the packing method to the manufacturer’s packing material information. Improper packing conditions can cause damage or completely destroy the material. -
Page 29: Slurry Preparation
YMC Europe GmbH Operation 6.4.1 Slurry preparation CAREFUL Formation of dust with dry packing material Lung irritation possible if packing material is inhaled. ► Read the material safety data sheet from the packing material manufacturer. ► Wear a dusk mask. -
Page 30: Slurry Column Packing Under Flow
YMC Europe GmbH Operation 6.4.2 Slurry column packing under flow The column is packed with the slurry method under flow as follows: 1. Remove the adjustable end piece Chapter 6.3.1, p. 27. 2. Tighten the O-ring of the fixed end piece Chapter 6.1, p. 26. -
Page 31: Slurry Column Packing With Sedimentation
By removing particles from the inner wall of the column body, a correct seal with the O-ring is guaranteed. 9. Do not move the glass column until packing is completed. YMC recommends covering the open column body during sedimentation with some plastic film. 10. Wait until the packing material has settled (sedimentation). -
Page 32: Column Test And Release Criteria
29. If a dead volume occurs, stop the pump and repeat step 23 to 26. 30. If no dead volume occurs, the pilot column is released Chapter 6.5, p. 32. 31. Equilibrate the glass column with the desired eluent and test the column performance. -
Page 33: Packing Adapter
YMC Europe GmbH Operation Packing adapter Due to the increased maximum column volume, the slurry concentration can be reduced. This way, the packing process is simplified. 1 Cap nut 8 Glass body 2 Washer 9 Cylinder screw 3 Set screw... -
Page 34: Glass Column Unpacking
YMC Europe GmbH Operation Glass column unpacking WARNING Glass column under high pressure Eye or skin injuries possible due to remaining pressure in the tubing or in the glass column. ► Stop the pump during working on the glass column. -
Page 35: Cleaning And Maintenance
Severe injuries due to breakage possible if unauthorised spare parts are used. ► Use only the manufacturer authorised spare parts. WARNING Faulty maintenance of the pilot column Severe injuries due to deterioration possible. ► Meet maintenance intervals. ► Perform the maintenance as described. -
Page 36: Notes For Cleaning-In-Place (Cip)
YMC Europe GmbH Cleaning and maintenance Notes for Cleaning-In-Place (CIP) CAREFUL Packing material Eye and skin injuries possible through contact with packing material. ► Wear the prescribed personal protective equipment. ATTENTION Cleaning with inappropriate solvents Material damage possible due to corrosion, leaching or deformation. -
Page 37: Cleaning And Maintenance Plan
YMC Europe GmbH Cleaning and maintenance Cleaning methods for CIP Treatment Type of contamination Sterilisation Depyrogenation Proteolysis of adsorbed Pepsin, pH 1.5 - 2 proteins Non-ionic detergents Removal of hydrophobic (Triton X-100, Tween 80) proteins and lipids Cationic detergents Removal of hydrophobic... -
Page 38: Cleaning
YMC Europe GmbH Cleaning and maintenance Cleaning WARNING Glass column under high pressure Eye or skin injuries possible due to the remaining pressure in the tubing or in the glass column. ► Stop the pump while working on the glass column. - Page 39 YMC Europe GmbH Cleaning and maintenance The glass column is cleaned as follows: 1. Remove the fixed end piece Chapter 6.3.2, p. 27. 2. Clean the fixed end piece with an appropriate solvent and / or flush the fixed end piece with 1 M sodium hydroxide.
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Page 40: Replacement Of Spare Parts
YMC Europe GmbH Cleaning and maintenance Replacement of spare parts ATTENTION Faulty assembly Damage to the glass body possible due to faulty assembly. ► Take care during the assembly of the glass body that the maximum torque is not exceeded: 2.2 Nm for columns with 100 mm ID, 4.2 Nm for columns with 140 and 200 mm ID and 7.0 Nm for columns with 300 mm ID. -
Page 41: Replacing The Glass Body
YMC Europe GmbH Cleaning and maintenance 7.4.3 Replacing the glass body ATTENTION Faulty assembly Damage to the glass body possible due to faulty assembly. ► Take care during the assembly of the glass body that the maximum torque is not exceeded: 2.2 Nm for columns with 100 mm ID, 4.2 Nm for columns with 140 and... -
Page 42: Troubleshooting
YMC Europe GmbH Troubleshooting Troubleshooting WARNING Incorrect maintenance Severe injuries during operation after incorrect maintenance possible if the column or column parts do not withstand the operating pressure. ► Only authorised and qualified personnel should carry out the maintenance. WARNING Incorrect replacement of spare parts Severe injuries due to breakage possible, if authorised spare parts are not used. - Page 43 Tubing connection leaks follows: 1. Tighten the fixing screws. 2. Check the tightness of tubing connection. 3. New column conditioning. If faults occur which are not listed here, please contact YMC for support. YMC Pilot Low pressure chromatography column 43 / 49...
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Page 44: Storage, Packaging And Transport
YMC Europe GmbH Storage, packaging and transport Storage, packaging and transport WARNING Solvents in the glass column Dangers possible due to liquids leaking from the column during transportation. ► Unpack and clean the column before transport. Storage For storage of packed columns, the chemical resistance of the wetted parts and of the packed stationary phase (please contact the manufacturer) should be considered. -
Page 45: Disposal
YMC Europe GmbH Disposal Disposal For the disposal of the glass column it has to be disassembled and disposed according to the national and other applicable environmental regulations. YMC Pilot Low pressure chromatography column 45 / 49... -
Page 46: Appendix
YMC Europe GmbH Appendix Appendix 11.1 Spare parts column body Pos. ID [mm] Part No. Description Material Borosilicate glass PI100/500 Glass body Borosilicate glass PI100/850 Glass body Borosilicate glass PI140/500 Glass body 3.33 Borosilicate glass PI140/850 Glass body Borosilicate glass... -
Page 47: Spare Parts End Pieces, Ab Version
YMC Europe GmbH Appendix 11.2 Spare parts end pieces, AB version Pos. ID [mm] Part No. Description Material 100, 140, 200, PT4,35FE6,35 Tubing PI100AB100E-1 O-ring plunger EPDM 100, 140, 200, Stainless steel PI001-1.4404 Spring 1.4404 100, 140, 200, M3x12 mm... -
Page 48: Spare Parts End Pieces, Sr Version
YMC Europe GmbH Appendix 11.3 Spare parts end pieces, SR Version Pos. ID [mm] Part No. Description Material 100, 140, 200, Stainless steel SS4,35NA6,35 Capillary 1.4404 PI100SR100K O-ring plunger Kalrez® 100, 140, 200, Stainless steel PI001-1.4404 Spring 1.4404 100, 140, 200, Stainless steel M4x8 –... -
Page 49: Index
YMC Europe GmbH Appendix 11.4 Index Cleaning Cleaning-In-Place Delivery contents Disposal Frit replacement Glass body replacement Packaging Packing Packing adapter Personal protective equipment Pressure test Protective equipment Resistance, chemical Signal word Signal word field Slurry Slurry preparation Slurry method sedimentation...
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