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YMC Pilot PLUS Manual

Low pressure chromatography column
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Original-
Manual
PLUS
YMC Pilot
Low pressure chromatography column
In order to avoid personal injuries and damage to equipment, please read this manual carefully and
act on the recommendations given. Keep this manual accessible for the user at all times.
26.03.2018
Version 1.3

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Summary of Contents for YMC Pilot PLUS

  • Page 1 Original- Manual PLUS YMC Pilot Low pressure chromatography column In order to avoid personal injuries and damage to equipment, please read this manual carefully and act on the recommendations given. Keep this manual accessible for the user at all times.
  • Page 2 YMC Europe GmbH YMC Europe GmbH Schöttmannshof 19 46539 Dinslaken, Germany +49 02064 427-0 Info@ymc.de www.ymc.de PLUS YMC Pilot low pressure chromatography column 2 / 49...

  • Page 3: Table Of Contents

    YMC Europe GmbH Contents Contents Information on manual ................... 5 General ........................ 5 Explanation of warnings ..................5 Structure of instructions ..................6 Guidelines ......................6 Figures ......................... 6 Safety ........................7 Intended use ......................7 General safety instructions .................. 7 Personnel requirements ..................
  • Page 4 YMC Europe GmbH Contents Disposal ......................... 45 Appendix ........................ 46 11.1 Spare parts column stand .................. 46 11.2 Spare parts adjustable end piece ..............46 Spare parts column body 70 – 100 mm ID ............47 11.3 Spare parts column body 140 – 200 mm ID ............48 11.4...

  • Page 5: Information On Manual

    YMC Europe GmbH Information on manual Information on manual General This manual includes important massages and information for the intended use of this pilot column. The manual must be kept accessible for the user at all times. In order to avoid injuries and damages, read this manual carefully prior to use and act on the recommendations given.

  • Page 6: Structure Of Instructions

    YMC Europe GmbH Information on manual Structure of instructions The instructions relate directly to an activity. They are structured in a task oriented manner. Always perform the individual steps in the order in which they are given. Instructions are structured as follows and are marked by the corresponding levels: ...

  • Page 7: Safety

    This section gives an overview of all the important safety aspects for the protection of PLUS personnel and for the safe and trouble-free handling of the YMC Pilot column. Other task-specific safety requirements are contained in the sections relating to the respective operation.

  • Page 8: Personnel Protective Equipment

    YMC Europe GmbH Safety Personnel protective equipment In order to reduce the risks during working on the column, always use the recommended personnel protective equipment. The following protective equipment is required: Symbol Phase of life Action • Column packing Protective gloves Operation •...

  • Page 9: Specifications And Overview

    YMC Europe GmbH Specifications and overview Specifications and overview Fields of application PLUS The YMC Pilot pilot column is especially designed for pilot scale use. The column is compatible with common BioLC separation modes (IEX, SEC, Affinity, IMAC, HIC, MMC) and aqueous conditions. Due to the metal-free and the minimal dead volumes, the column is perfectly suitable for the separation of biomolecules (e.g.

  • Page 10: Chemical Resistance

    YMC Europe GmbH Specifications and overview Chemical Resistance The chemical resistance depends on the wetted materials used Chapter 3.2, S. 9. If the desired solvent is not listed, YMC will be pleased to advise you. Material Borosilicate PTFE PEEK glass 3.3 Fluoro- Lösungsmittel...

  • Page 11: Overview

    YMC Europe GmbH Specifications and overview Overview A parts list with all wetted parts and non-wetted parts is in the appendix. Adjustable end piece Column body Column stand PLUS The YMC Pilot column consists of the adjustable end piece, the column body and the column stand.

  • Page 12: Adjustable End Piece

    YMC Europe GmbH Specifications and overview 3.4.1 Adjustable end piece Tubing Star screw Spindle Closing disc (AEP) Rotary Handle Connecting flange The adjustable end piece consists of a closing disc and the piston. The piston consists of the spindle, the connecting flange, the plunger, the frit and the profile gasket. A tubing goes through the spindle to the connecting flange.

  • Page 13: Column Body

    YMC Europe GmbH Specifications and overview 3.4.2 Column body Cylinder screw Connector Column disc Glass body Connector drainage tubing Drainage tubing Holding bar Closing disc (CB) The glass body is fixed between the column disc and the closing disc by the rods. The glass body has a visible scale.

  • Page 14: Column Stand

    YMC Europe GmbH Specifications and overview Frit column body Connector without cap nut Frit (CB) Plunger (CB) Ring gasket A Ring gasket B 3.4.3 Column stand Levelling feet Frame disc Main pillar Locking bolts With the locking bolts in the frame disc the column body is attached to the column stand.

  • Page 15: Delivery

    – YMC Quality Control / Leakage Tests – Certificate of conformity FDA/BSE/TSE/USP Class VI Delivery inspection YMC recommends retaining and storing the original packaging and the foam cushion so that the column can always be shipped safely. Delivery inspection  Delivery inspection is performed as follows: 1.

  • Page 16: Requirements For Operation

    YMC Europe GmbH Requirements for operation Requirements for Operation Pilot column set up Required devices: • Drainage tubing • Collecting vessel  The pilot column is set up as follows: 1. Make sure that the pilot column is standing on fixed ground.
  • Page 17 YMC Europe GmbH Requirements for operation Standard procedure pressure test 1 Pilot column 4 Pressure gauge 2 Back pressure regulator 5 Gate valve 3 Gate valve 6 Pump  The pressure test should be performed as follows: 1. Assemble the system according to the schematic overview of a pressure test.

  • Page 18: Operation

    YMC Europe GmbH Operation Operation WARNING Risk of explosion Severe injuries possible due to glass splinters. ► Never put the glass column under gas pressure! In order to guarantee safe operation, the pilot column must be used for the intended use only.

  • Page 19: Pilot Column Packing

    YMC Europe GmbH Operation Pilot column packing CAREFUL Packing material Eye and skin injuries possible through contact with packing material. ► Wear the prescribed personnel protective equipment. Two different procedures are common for slurry packing the column: • under flow, •...

  • Page 20: Slurry Column Packing Under Flow

    YMC Europe GmbH Operation 6.2.2 Slurry column packing under flow  The column is packed with the slurry method under flow as follows: 1. Remove the adjustable end piece Chapter 6.1, S. 18. 2. Check the drainage tubing for correct connection.
  • Page 21 YMC Europe GmbH Operation 15. Turn the closing disc of the adjustable end piece half turn in the DOWN direction.  The adjustable end piece is ready for insertion into the column body. 16. Put the piston of the adjustable end piece in a smooth angle opposite the drain function on the column body.
  • Page 22 YMC Europe GmbH Operation 18. Align the marks on the closing disc (AEP) and column disc. 19. Screw the 4 star screws continuously in a diagonal pattern to each other. 20. Open the valve in the tubing on the adjustable end piece.
  • Page 23 33. Remove liquid above the connecting flange through the two holes in the adjustable end piece.  YMC recommends flushing this area if high salt concentrations are used. 34. Equilibrate the pilot column with the desired eluent and test the column performance.

  • Page 24: Slurry Column Packing With Sedimentation

     By removing particles from the inner wall of the glass body, a correct seal with the profile gasket is guaranteed. 14. Do not move the pilot column anymore.  YMC recommends covering the open column body during sedimentation with some plastic film. 15. Wait until the packing material has settled (sedimentation).
  • Page 25 YMC Europe GmbH Operation 18. Turn the closing disc of the adjustable end piece half turn in the DOWN direction.  The adjustable end piece is ready for insertion into the column body. 19. Put the piston of the adjustable end piece in a smooth angle opposite the drain function on the column body.
  • Page 26 YMC Europe GmbH Operation 21. Align the marks on the closing disc (AEP) and column disc. 22. Screw the 4 star screws continuously in a diagonal pattern to each other. 23. Open the valve in the tubing on the adjustable end piece.

  • Page 27: Column Test And Release Criteria

    The column test and the release criteria are selected with regards to the later application field. The preparation of the required specifications for the final usage of a method or for the purification of a target molecule is determined individually. YMC will gladly support you.

  • Page 28: Pilot Column Unpacking

    YMC Europe GmbH Operation Pilot column unpacking WARNING Pilot column under high pressure Eye or skin injuries possible due to remaining pressure in the tubing or in the pilot column. ► Stop the pump during working on the pilot column.
  • Page 29 YMC Europe GmbH Operation 8. Loosen the locking bolts and lock them by turning slightly. 9. Lift the column body vertical from the column stand using two holding bars which are vertically above each other. 10. Pour any unpacked material and/or solvent into a collecting vessel.

  • Page 30: Cleaning And Maintenance

    YMC Europe GmbH Cleaning and maintenance Cleaning and maintenance WARNING Incorrect maintenance Severe injuries when used after incorrect maintenance is possible, if the column or column parts do not withstand the operating pressure. ► Only authorised and qualified personnel should carry out the maintenance.

  • Page 31: Notes For Cleaning-In-Place (Cip)

    YMC Europe GmbH Cleaning and maintencance Notes for Cleaning-In-Place (CIP) CAREFUL Packing material Eye and skin injuries possible through contact with packing material. ► Wear the prescribed personnel protective equipment. ATTENTION Cleaning with inappropriate solvents Material damage possible due to corrosion, leaching or deformation.

  • Page 32: Cleaning And Maintenance Plan

    YMC Europe GmbH Cleaning and maintencance Cleaning methods for CIP Treatment Type of Sterilisation Depyrogenation contamination Pepsin, pH 1.5 - 2 Proteolysis of adsorbed proteins Non-ionic detergents (Triton X- Removal of hydrophobic 100; Tween 80) proteins and lipids Cationic detergents, pH 9 - 11...

  • Page 33: Cleaning

    YMC Europe GmbH Cleaning and maintencance Cleaning WARNING Pilot column under high pressure Eye or skin injuries possible due to remaining pressure in the tubing or in the pilot column. ► Stop the pump while working on the pilot column.
  • Page 34 YMC Europe GmbH Cleaning and maintencance  The pilot column is cleaned as follows: 1. Remove the adjustable end piece Chapter 6.1, S. 18. 2. Clean the outer wall of the column body. 3. Clean the column body with an authorised cleaning agent and a wetted, lint- free rag.
  • Page 35 YMC Europe GmbH Cleaning and maintencance 9. Connect the pump to the tubing in the column body. 10. Start the pump. 11. Flush the column body with an appropriate solvent and/or 1 M sodium hydroxide. 12. Stop the pump. 13. Disconnect the pump from the tubing in the column body.

  • Page 36: Replacement Of Spare Parts

    YMC Europe GmbH Cleaning and maintencance Replacement of spare parts ATTENTION Faulty assembly Damage to the glass body possible due to faulty assembly. ► Take care during the assembly of the glass body that the maximum torque of 5 Nm for columns with 70 and 100 mm ID respectively 10 Nm for column with 140 mm and 200 mm ID are not exceeded.

  • Page 37: Replacing The Glass Body

    YMC Europe GmbH Cleaning and maintencance 7.4.2 Replacing the glass body ATTENTION Faulty assembly Damage to the glass body possible due to faulty assembly. ► Take care during assembly of the glass body that the maximum torque of 5 Nm for columns with 70 and 100 mm ID respectively 10 Nm for column with 140 mm and 200 mm ID are not exceeded.
  • Page 38 YMC Europe GmbH Cleaning and maintencance 5. Remove flat gasket. 6. Remove glass body carefully. 7. Position the new glass body on the ring gasket A. 8. Check that the glass body is concentric with the gaskets. 9. Put the flat gasket corresponding to the structure on the top edge of the glass body.
  • Page 39 YMC Europe GmbH Cleaning and maintencance 10. Adjust the column disc over the rods and lower it onto the glass body. 11. Check that the flat gasket is in the groove in the column disc. 12. Check that the glass body is concentric with the column disc.

  • Page 40: Frit Replacement Column Body

    YMC Europe GmbH Cleaning and maintencance 7.4.3 Frit replacement column body ATTENTION Faulty assembly Damage to the glass body is possible due to faulty assembly. ► Take care during assembly of the glass body that the maximum torque of 3 Nm is not exceeded.
  • Page 41 YMC Europe GmbH Cleaning and maintencance 5. Remove the ring gasket A. 6. Remove the frit. 7. Insert the frit. The distribution profile faces the plunger. 8. Reattach the ring gasket A and press firmly in position. 9. Replace the fixed end piece into the closing disc (CB).

  • Page 42: Troubleshooting

    YMC Europe GmbH Troubleshooting Troubleshooting WARNING Incorrect maintenance Severe injuries during operation after incorrect maintenance possible if the column or column parts do not withstand the operating pressure. ► Only authorised and qualified personnel should carry out the maintenance. WARNING Incorrect replacement of spare parts Severe injuries due to breakage possible, if not authorised spare parts are used.
  • Page 43 Tubing connection leaks proceed as follows: Tighten the fixing screw. Check tightness of tubing connection. New column conditioning. If faults occur which are not listed here, please contact YMC for support. PLUS YMC Pilot low pressure chromatography column 43 / 49...

  • Page 44: Storage, Packaging And Transport

    YMC Europe GmbH Storage, packaging and transport Storage, packaging and transport WARNING Solvents in the pilot column Dangers possible due to liquids leaking from the column during transportation. ► Unpack and clean the column before transport. Storage For storage of packed columns, the chemical resistance of the wetted parts and of the packed stationary phase (please contact the manufacturer) should be considered.

  • Page 45: Disposal

    YMC Europe GmbH Disposal Disposal For the disposal of the pilot column it has to be disassembled and disposed according to the national and other applicable environmental regulations. PLUS YMC Pilot low pressure chromatography column 45 / 49...

  • Page 46: Appendix

    YMC Europe GmbH Appendix Appendix 11.1 Spare parts column stand Pos. ID [mm] Part-No. Description Material 70 - 100 00128 Sliding disc Teflon (PTFE) 140 - 200 000203 Sliding disc Teflon (PTFE) 11.2 Spare parts adjustable end piece Pos. ID [mm] Part-No.

  • Page 47: Spare Parts Column Body 70 - 100 Mm Id

    YMC Europe GmbH Appendix Spare parts column body 70 – 100 mm ID 11.3 Pos. ID [mm] Length glass Part-No. Description Material body [mm] EPDM white 000006 Flat gasket (0082E) Borosilicate 000007 Glass body glass 3.3 SI15,0LM21,0 Drainage tubing Silicone...

  • Page 48: Spare Parts Column Body 140 - 200 Mm Id

    YMC Europe GmbH Appendix Spare parts column body 140 – 200 mm ID 11.4 Pos. ID [mm] Length glass Part-No. Description Material body [mm] EPDM white 000141 Flat gasket (0082E) Borosilicate 000147 Glass body glass 3.3 75 Fluoroprene 000140 Ring gasket A...

  • Page 49: Index

    YMC Europe GmbH Index Index Cleaning Cleaning-In-Place Delivery contents Disposal Frit replacement adjustable end piece column body Glass body replacement Packaging Packing Presonnel protective equipment Pressure test Protective equipment Resistance, chemical Signal word Signal word field Slurry Slurry preparation Slurry method sedimentation...

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