AZENTA Cryo Store Pico Hardware Manual
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AZENTA Cryo Store Pico Hardware Manual

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Cryo Store Pico
Hardware Manual
384478 Revision B

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  • Page 1: Cryo Store Pico Hardware

    Cryo Store Pico Hardware Manual 384478 Revision B...
  • Page 2 © 2023 Azenta, Inc. All rights reserved. The information included in this manual is proprietary information of Azenta, Inc. and is provided for the use of Azenta, Inc. customers only and cannot be used for distribution, reproduction, or sale without the express written permission of Azenta, Inc.
  • Page 3 North America +1-888-2AZENTA (+1-888-229-3682) Europe +44-161-777-2000 www.azenta.com Japan +81-3-6628-2950   For Technical Support: Location Contact Number Website North America +1-800-379-7221 Europe +41-31-770-7373 / +44-161-777-2107 www.azenta.com Japan +81-3-6628-2950 Email the Azenta Help Desk at Service.Products@Azenta.com.   Copyright © 2023, Azenta, Inc.

  • Page 4: Revision History

    Cryo Store Pico Hardware Cryo Store Pico Revision History Part Number: 384478 Rev. B Revision History Part Number: 384478 Cryo Store Pico Hardware Manual Revision Date Revision A 17 FEB 2023 Revision B 12 APR 2023 Copyright © 2023, Azenta, Inc.

  • Page 5: Table Of Contents

    Azenta Life Sciences Cryo Store Pico Hardware Part Number: 384478 Rev. B Revision History   Table of Contents Cryo Store Pico Hardware Revision History 1. Safety Explanation of Hazard Alerts Safety Text Safety Icons Signal Words and Color Alert Example...
  • Page 6 Cryo Store Pico Hardware Cryo Store Pico Revision History Part Number: 384478 Rev. B LN2 Usage Temperature Measurement Battery Backup 6. Backup Freezer ControllerOverview Home Screen Main Settings Screen Setpoint Settings Screen Default Settings 7. Preventative Maintenance Preventative Maintenance Schedule...

  • Page 7: Safety

    1. Safety This chapter describes the safety guidelines for the Azenta Life Sciences Cryo Store Pico. All personnel involved in the operation or maintenance of the product should be familiar with the safety precautions outlined in this chapter.                  Read the Safety Chapter Failure to review the Safety chapter and follow the safety warnings can result in death or serious injury.

  • Page 8: Explanation Of Hazard Alerts

      State how to avoid the hazard. Safety Icons   Hazard alerts contain safety icons that graphically identify the hazard.   The safety icons in this manual conform to ISO 3864 and ANSI Z535 standards. Copyright © 2023, Azenta, Inc.

  • Page 9: Signal Words And Color

    Indicates a situation or unsafe practice which, if not avoided, may result in equipment damage.   Notice signal word is white on blue background with no icon.   Copyright © 2023, Azenta, Inc.

  • Page 10: Alert Example

    Table 1-1: Warning Sample Description Number Description 1.   How to Avoid the Hazard 2.   Source of Hazard and Severity 3.   General Alert Icon 4.   Signal Word 5.   Type of Hazard 6.   Hazard Symbol(s) Copyright © 2023, Azenta, Inc.

  • Page 11: General Safety Guidelines

    Only Azenta parts are authorized to be used when configuring or repairing this system.                  Proper Operation  Improper use of the Cryo Store Pico may cause personal injury or equipment damage.   Do not operate the unit before it is properly installed.  ...
  • Page 12 The use of this product in an earthquake prone environment may cause equipment damage or personal injury. The user is responsible for determining whether the product is used in an earthquake prone environment and installing the appropriate seismic restraints in accordance with local regulations.   Copyright © 2023, Azenta, Inc.

  • Page 13: Safety And Identification Labels

    Part Number: 384478 Rev. B Safety and Identification Labels Safety and Identification Labels Safety labels are placed on the Cryo Store Pico to identify hazards. Identification labels provide information about the product. Safety Labels provide instructions on how to avoid the hazard.  ...
  • Page 14 How to avoid the hazards: Do not remove the computer stand while powered. (Hazard) Label Type or Name: Read the Technical Manual p/n 248647 Qty: 2 Location: Rear door, tower How to avoid the hazards: Read the user manual before operating unit. Copyright © 2023, Azenta, Inc.

  • Page 15: Location Of Labels

    Crush/Moving Part:  on the base plate of the theta, on the base plate of the Y-axis near the bearing, on 3.   the tower. 4.   Extreme Cold: on the sample counter 5.   Shock Hazard: on the electrical box 6.   Arc Flash Hazard: on the electrical box Copyright © 2023, Azenta, Inc.

  • Page 16: Interlocks

    Safety interlocks protect personal safety. Operational interlocks protect the equipment's safety. Additional interlocks may be added by the user. The system comes with interlocks on the front and rear doors. If you open either of these doors, automation shuts down.   Copyright © 2023, Azenta, Inc.

  • Page 17: Mechanical Hazards

    Do not operate the product without the guards and protective covers in place and all safety interlocks enabled.                  Cutting or Severing This product contains sharp metal edges. Improper interaction may result in cuts.   Don appropriate PPE when interacting with the system.   Copyright © 2023, Azenta, Inc.
  • Page 18 Rotating elements in the T-latch may result in bruises.   Be aware of the potential movement area of the product whenever power is applied.   Do not operate the system without the guards and protective covers in place and all safety interlocks enabled. Copyright © 2023, Azenta, Inc.

  • Page 19: Electrical Hazards

    Refer the units specifications for the actual system power requirements and use appropriate precautions.                    Arc Flash Hazard An arc flash can cause death or serious personal injury.   To avoid arc flashes, disconnect the power before troubleshooting.   Don the appropriate PPE. Copyright © 2023, Azenta, Inc.

  • Page 20: Thermal Hazards

    This product contains heaters at the neck to prevent ice build-up. Contact with extreme heat can cause burns.   Don the appropriate PPE when operating the system.   Do not touch components without the use of insulated gloves or appropriate personal protective equipment. Copyright © 2023, Azenta, Inc.

  • Page 21: Environmental Hazards

    Boiling point at 1 atm -195.8°C | 77.4 K | -320.4°F Density, liquid at boiling point 1.78 lb/L | 50.4 lb/scf | 808 g/L | 808 kg/m3 Heat of vaporization, liquid 199.1 kJ/kg | 85.60 Btu/lb   Copyright © 2023, Azenta, Inc.

  • Page 22: Frostbite Hazard

    Long exposure to this noise may cause hearing damage. Nitrogen has a liquid to gas expansion ratio of 1 to 694. Overpressurization can occur if LN is trapped in a closed container and can result in death or serious injury. Copyright © 2023, Azenta, Inc.

  • Page 23: Thermal Load

    The freezer itself is a stainless steel, vacuum insulated vessel with an aluminum inner turn tray. Once freezer has been decontaminated and any prohibited components removed, the freezer may be disposed of or recycled according to local regulatory guidelines. Copyright © 2023, Azenta, Inc.

  • Page 24: Regulatory Compliance

    Regulatory Compliance Declaration of Conformity, Machinery Directive The Cryo Store Pico meets the requirements of the Machinery Directive 2006/42/EC, Electromagnetic Compatibility Directive 2014/30/EU, and 2011/65/EU Directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
  • Page 25 Azenta Life Sciences 1. Safety Part Number: 384478 Rev. B Regulatory Compliance     Copyright © 2023, Azenta, Inc.

  • Page 26: Declaration Of Conformity, Supply Of Machinery (Safety) Regulations 2008

    Part Number: 384478 Rev. B UK Declaration of Conformity, Supply of Machinery (Safety) Regulations 2008 The Cryo Store Pico meets the requirements of the relevant provisions of Supply of Machinery (Safety) Regulations 2008, Electromagntic Compatibility Regulations 2016, and the Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equipment Regulations 2012.
  • Page 27 Azenta Life Sciences 1. Safety Part Number: 384478 Rev. B Regulatory Compliance     Copyright © 2023, Azenta, Inc.

  • Page 28: Introduction

    2. Introduction This manual details the operation and maintenance of the Cryo Store Pico hardware. Read before using this equipment. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired Cryo Store Picos are designed for safe and efficient storage of biological samples in dry liquid nitrogen ) vapor at -190°C (-310°F).

  • Page 29: Terminology

    System Controller Software is not running on the system controller. Nest Slot in the Cryo Store Pico freezer in which the racks fit. Tall storage unit that holds the cryoboxes in the Cryo Store Pico freezer Rack nests. General term for tubes, vials, and similar containers that can fit in Sample standard cryoboxes.

  • Page 30: Access Doors And Ports

    Controls manual measurement port accessibility access port Bottom rear door Allows service to access freezer controller and UPS Allows service to access freezer for emergency maintenance, system con- Upper front door troller, and emergency tools Copyright © 2023, Azenta, Inc.

  • Page 31: Product Information

    3. Product Information Store Specifications Parameter Specification Width 42.7 inches (108.5 cm) Depth 56.8 inches (144.3 cm) Height 95.5 inches (242.6 cm) Weight (fully loaded) 1433 lbs (650 kg) Rack capacity capacity 93 L Hold time 10 days Part Number: 384478 Rev. B...

  • Page 32: Control System

    Dry Alarm Contacts NO/NC 2 x Pt-100 RTD | -200°C to 70°C | Temperature sensors EL-0133 ±1.5°C Level sensors Differential Pressure | ±0.5 in (1.3 cm) EL-0018 Battery backup 2 x 12VDC | 7AH | Lead-acid EL-0126 Copyright © 2023, Azenta, Inc.

  • Page 33: Plumbing System

    Part Number: 384478 Rev. B Plumbing System Plumbing System The plumbing system for the Cryo Store Pico safely and efficiently transfers LN from the supply system to the freezer and transmits the LN differential pressure level signal to the controller. It is installed at the rear of the freezer behind the controller enclosure.

  • Page 34: Facility Requirements

    Relative humidity Less than 50%, non-condensing Seismic restraints may be required in earthquake prone areas. Please follow local stand- Seismic restraint ards and regulations. Contact Azenta Life Sciences for more information. Maximum altitude 2000 m above sea level Maximum pollution...

  • Page 35: Installation

    4. Installation The installation of the Cryo Store Pico is completed by certified Azenta Life Sciences service personnel. Please contact Azenta service regarding installation information. Part Number: 384478 Rev. B...

  • Page 36: Operation

    5. Operation Vapor Storage   Cryopreservation is the storage of material below -135°C (-211°F) in order to provide longevity to biospecimens. Around -135°C is referred to as the glass transition (Tg) of polyol’s water solution. It is generally accepted that below Tg, biological activity essentially ceases. At these temperatures, enzymatic activity slows to a stop while frozen water molecules inside cells can no longer move freely and participate in chemical reactions.

  • Page 37: Ln2 Level Measurement

    This can be used for independent third-party level monitoring or troubleshooting, or in the event of a main line blockage. This second annular line is offset from the main lines, located higher up in the freezer.  Copyright © 2023, Azenta, Inc.

  • Page 38: Ln2 Level Control

    Persistent, excessive, or patterned condensation and frost along the body of the freezer could be indicative of an insulation issue. Please contact Azenta Life Sciences service for help. Fill Functionality Fill functionality is intentionally disabled or paused under certain circumstances for user safety and convenience.

  • Page 39: Ln2 Usage

    If main power is lost, the freezer automatically switches to battery power and the Running on Battery Backup alarm is initiated. While running on battery backup, full monitoring, logging, and alarm functionality remain active; however, autofill is disabled to save power. Copyright © 2023, Azenta, Inc.

  • Page 40: Backup Freezer Controlleroverview

    The Home screen displays the current freezer status and storage conditions. This screen displays the freezer temperatures, LN level, and LN usage. The units shown reflect the units selected within the BioStore™ System Controller Software. Figure 6-1: Home screen when Cryo Store Pico software is on Part Number: 384478 Rev. B...

  • Page 41: Main Settings Screen

    To return to the Home screen, press the Home icon in the bottom left corner. To access the Setpoint Settings, press the Setting icon in the bottom right corner of the Main Settings screen. Figure 6-3: Main Settings screen Copyright © 2023, Azenta, Inc.

  • Page 42: Setpoint Settings Screen

    8888). The adjustable settings include fill setpoints, temperature alarm thresholds, max fill and purge timers, and purge temperature cutoff. To return to the Home screen, press the Home icon in the bottom left corner. Figure 6-4: Setpoint Settings screen Copyright © 2023, Azenta, Inc.

  • Page 43: Default Settings

    Low level alarm Low level fill - 0.5 in (1.3 cm) High temperature alarm -150°C Fill time alarm 60 min Purge time 5 min Purge temperature -50°C Temperature | Level units °C | in Password 8888 Copyright © 2023, Azenta, Inc.

  • Page 44: Preventative Maintenance

    The following maintenance schedule is a quick reference for standard user maintenance of the Azenta Life Sciences Cryo Store Pico. If additional procedures are required during the performance of any procedure, they will be provided. Both the page number for procedures included in this manual and the recommended frequency for performing these procedures are provided below.

  • Page 45: Cleaning

    Calibration/verification of the freezer controller annual preventative maintenance.   Cleaning The exterior cabinetry of the Cryo Store Pico can be wiped down with a soft cloth and the following chemicals recommended by Azenta Life Sciences:   Hydrogen peroxide (6% solution)  ...

  • Page 46: Verifying Ln2 Supply, Temperature, And Ln2 Usage

    Initiate a fill on at least one freezer. The supply system should be able to maintain appropriate pressure 10.   throughout the duration of the fill cycle. 11.   If the fill is insufficient, replenish your LN supply. 12.   Check temperature and LN usage on the freezer controller.   Copyright © 2023, Azenta, Inc.

  • Page 47: Removing Frost And Ice

    Inspect the LN piping assembly for any visible damage or hardened ice blocks. NOTE: Frost build-up is normal. If you find hardened ice blocks, contact Azenta Life Sciences and DO 20.   NOT PROCEED WITH NEXT STEPS.

  • Page 48: Plumbing Leak Check

    Once all connections have been tightened or replaced, re-check for leaks.   Z-Brake Override Battery Replacement Step Action 1.   Open the Cryo Store Pico industrial design door. 2.   Replace the (3) 9V batteries in the Z-brake override battery packs. 3.   Ensure the Z-brake override is functional.  ...

  • Page 49: Complete Thaw And Moisture Removal

    NOTE: If moisture is not completely removed from the freezer and annular lines, ice forms when LN is reintroduced into the freezer. Ice blockage in the freezer or annular lines interferes with proper function of the freezer and level sensing system. Copyright © 2023, Azenta, Inc.

  • Page 50: Troubleshooting

    Slow fill   Freezer fill valve(s) connection / faulty Max fill time alarm level fill setpoint.   Plumbing assembly leak If problem persists, contact Azenta   Auto fill temporarily disabled (see "LN2 Life Sciences Technical Support for Level Control" on page 38) assistance.
  • Page 51 8. Troubleshooting Part Number: 384478 Rev. B Problem Possible Causes Corrective Actions Check for temp probe error. Incorrect temp / sensor If problem persists, contact Azenta   Temp probe connection / faulty Life Sciences Technical Support for error assistance.   No level signal  ...

  • Page 52: Emergency Manual Override Procedure

    Emergency Manual Override Procedure Part Number: 384478 Rev. B Emergency Manual Override Procedure Step Action Open the Pico front and side doors.   1.   Turn off the automation power to the system.   2.   Copyright © 2023, Azenta, Inc.
  • Page 53 If the push-out is extended, pull the back of the push-out until you reach the hard stop.   3.   Pull the Z-brake override switch inside the tower until you hear a click.   4.   Copyright © 2023, Azenta, Inc.
  • Page 54 Use a wrench on the flat part of the ball screw to lower the hand brake lever until you can reach it.   5.   Using the handle on the Z-axis, pull the Z-axis down into the freezer.   6.   Copyright © 2023, Azenta, Inc.
  • Page 55 When the rack is fully seated in the freezer, open the access module door and pull the latch handle to the right.   7.   Using the handle on the Z-axis, push the Z-axis up until the gripper is completely out of the freezer and is inside the access module.   8.   Copyright © 2023, Azenta, Inc.
  • Page 56 Cryo Store Pico Emergency Manual Override Procedure Part Number: 384478 Rev. B Step Action Re-secure the ball screw.   9.   Remove the (2) screws from the sample counter, then remove the sample counter.   10.   Copyright © 2023, Azenta, Inc.
  • Page 57 Part Number: 384478 Rev. B Emergency Manual Override Procedure Step Action Close the freezer lid.   11.   Close the access module door.   12.   13.   Contact service using the numbers listed in"Contact Technical Support" on page Copyright © 2023, Azenta, Inc.

  • Page 58: Other Documentation Resources

    Part Number: 384478 Rev. B Other Documentation Resources This manual (Cryo Store Pico Hardware Operator Manual) contains information on the Cryo Store Pico safety, hardware operation, and maintenance. For more information on the software used to operate the Cryo Store Pico (BioStore™ System Controller Software), see the following documentation: Part ...

  • Page 59: Appendices

    9. Appendices This chapter contains the appendices of the manual. Part Number: 384478 Rev. B...

  • Page 60: Appendix A: Weee Statement (European Union)

    Such treatment is the most effective means of protecting the customer’s environment. The waste collection, reuse, recycling, and recovery programs available to Azenta Life Sciences customers vary by customer location. Please contact the responsible body (e.g., your laboratory manager) for information about local requirements.

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