Related Manuals for Nahita GET-S
Summary of Contents for Nahita GET-S
- Page 1 Operation manual of Thermal Cycler GET-S Code ZFD019 Operation manual Thermal Cycler GET-S Code ZFD019 Please read this manual carefully before operating Thermal Cycler ! Document Version: June 26, 2023, Version 1.2...
-
Page 2: Table Of Contents
Operation manual of Thermal Cycler GET-S Code ZFD019 CONTENTS CONTENTS ............................. II Chapter 1 Safety Guidelines ......................1 Chapter 2 Installation ........................2 2.1 Package Contents Verification .................... 2 2.2 Normal Operating Conditions ..................... 2 2.3 Transportation and Storage Requirements ................2 2.4 Power Requirements ...................... -
Page 3: Chapter 1 Safety Guidelines
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 1 Safety Guidelines 1. Instrument Safety Warning Labels Icon Meaning High temperature warning: To avoid burning, please don’t touch directly all areas labeled with safety warning and the hot areas described in manual. -
Page 4: Chapter 2 Installation
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 2 Installation 2.1 Package Contents Verification Please open box and check the following list when you receive the GE9612T-S PCR instrument. Name Quantity Instrument Power Line (single-phase 3-wire) Spare fuse（8A ，250V）... -
Page 5: Chapter 3 Instrument Characteristics
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 3 Instrument Characteristics 3.1 Instrument Construction A. Host machine Status LED Reaction module bay LCD display Air vents USB A port Figure 1. Frontal view of the thermal cycler. ● Reaction module bay —... - Page 6 Operation manual of Thermal Cycler GET-S Code ZFD019 B. Reaction module Unlock pin Lid pressure adjustment wheel Lid pull Connector Cooling fins Figure 3. The lid and cooling fins of a 96-well reaction module. ● — Lid pressure adjustment wheel adjust the lid pressure ●...
- Page 7 Operation manual of Thermal Cycler GET-S Code ZFD019 C. High Performance Smart Lid To achieve optimum pressure on the tubes GE9612T-S is equipped with a height adjustable heated lid. Close the lid: After the samples have been placed in the block close the lid. Turn the wheel clockwise until you hear a clicking noise.
- Page 8 Operation manual of Thermal Cycler GET-S Code ZFD019 Unlock pin Lid pressure adjustment wheel Release lid in upper position: 1) press pin 2) carefully turn wheel while holding the pin down CLOCKWISE, until you feel normal resistance (no more clicking noise, clutch is released). Release pin and turn lid down, until the clutch mechanism is activated (clicking noise, optimum pressure applied).
-
Page 9: Specialty
Operation manual of Thermal Cycler GET-S Code ZFD019 3.2 Specialty 1. Each thermal block has 3 independent temperature control sensors and 6 peltier heating units to ensure accurate and uniform temperature across block surface, and provide users for replicating previous condition set-up. -
Page 10: Performance
Operation manual of Thermal Cycler GET-S Code ZFD019 3.3 Performance Model GE9612T-S Capacity 96×0.2ml Temperature Range 0-105℃ Max. Heating Rate 4.5℃/s Max. Cooling Rate 4℃/s Uniformity ≤±0.2℃ Accuracy ≤±0.1℃ Display Resolution 0.1℃ Temperature Control Block\Tube Ramping Rate Adjustable 0.1-4.5℃ Gradient Temp. Range 30-105℃... -
Page 11: Chapter 4 Operation Instruction
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 4 Operation instruction 4.1 Power on Connect power cord to the back of instrument then plug into power source and switch to "-". . When the instrument is on, the buzzer should be heard and the LCD screen lights up to show the Boot screen (Figure5). -
Page 12: Menu Structure
Operation manual of Thermal Cycler GET-S Code ZFD019 4.2 Menu structure Main menu New File Low temperature Home High temperature Advanced Gradient Clear all Increment Save as Extend Ramp Rate Flie Library Home New directory Directory option File option Edit... -
Page 13: File Instructions
Operation manual of Thermal Cycler GET-S Code ZFD019 4.3 File instructions File is composed by Temperature Step and Cycle Step. Each File can hold up to 30 Steps, Temperature Step contains Temperature, Time, Gradient, Ramp Rate, Temperature Increment and Time Extend. The maximum number of Cycles is 199 times. - Page 14 Operation manual of Thermal Cycler GET-S Code ZFD019 In the New File screen, touch “+Temp” button to add a Temperature Step, touch “+Cycle” button to add Cycle Step (GOTO step), use the pop-up numeric keypad to enter a new value for the target temperature or hold time.
- Page 15 Operation manual of Thermal Cycler GET-S Code ZFD019 After entering Gradient values the gradient distribution table (Figure 11) will be shown at bottom of the screen. Touch “OK” to save changes and return Edit File screen (Figure 12). Figure 11. Set the Gradient Figure 12.
- Page 16 Operation manual of Thermal Cycler GET-S Code ZFD019 button, the symbol ∞ (infinite) will come out. Gradient range Lower: The lower temperature in the Instructs the thermal cycler to gradient. Enter a number between 30.0 ramp to the target temperature and 105.0℃...
- Page 17 Operation manual of Thermal Cycler GET-S Code ZFD019 3). Insert a Step Insert a step if a new temperature, Cycle (GOTO), or gradient step is needed. Follow these instructions to insert a step to the right of a preexisting step.
-
Page 18: Delete File
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 13. Name file and select the path Figure 14. New file in the library. 4.3.2 Delete file and the In the File Library screen (Figure8), touch Options button to select the file to delete, query dialog window will pop up when touching “Delete”... -
Page 19: Copy File
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 15. Delete a file. 4.3.3 Copy file In the File Library screen (Figure8), touch Options button to select the file to copy and touch copy button. Then enter the save path and touch Paste button to finish copy. - Page 20 Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 16. Run file setup. In the Run File Setup screen (Figure16), select the Block which needs to run, enter the not necessary to be in block temperature control mode volume ( ).
- Page 21 Operation manual of Thermal Cycler GET-S Code ZFD019 running. 3). Stop Touch “Stop” button to stop a running file. 4). Skip Touch” Skip” button to jump to next step. 5). Go to other screen Touch Home button to back the Home screen, or touch Circle in the middle of the screen(Fig.18) to enter the running file screen (Fig.17).
-
Page 22: Setting
Operation manual of Thermal Cycler GET-S Code ZFD019 4.4 Setting In the Home screen, touch Setting button to enter the Setting screen (Figure19). Figure 19. Setting menu 4.4. 1 Run Setting In the Setting screen (Figure19), select the Run Setting to enter the Run Setting screen (Figure20). -
Page 23: System Setting
Operation manual of Thermal Cycler GET-S Code ZFD019 The block The temperature control mode contains block control mode and tube control mode. control mode is suitable for performing normal PCR and the tube control mode is for experiments that require higher environment conditions. -
Page 24: Glp Report
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 21. E-mail s etting 1). Status of email alert After program is over it will automatically send email if chose Enable. 2). Test email Test email function is to test whether email can send or not. -
Page 25: Software Update
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 23. Local information. 4.5.6 Software update Copy the new software to the root directory; insert the USB flash disk into the USB port, then touch “Software update”, return to Android desktop when updating, and immediately reopen the updated App . -
Page 26: Tm Calculator
Operation manual of Thermal Cycler GET-S Code ZFD019 and gradient range (Fig.23). Figure 23. Gradient calculator. 4.5.2 Tm Calculator Touch the fields to enter concentrations and the primer sequences,then touch Calculate Tm (Fig.24) to calculate the melting temperatures,Maximum input for each primer sequence is 30 . -
Page 27: Log In
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 25. Incubate wizard. In the Incubate wizard screen (Fig.25), select the desired block and enter the block temperature and the hold time. Then touch Start button to enter Incubate Running screen (Fig.26). -
Page 28: New User
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 27. User management. 4.7.1 New User In the User screen (Fig.27), touch New user button to enter the New User screen (Fig.28).Enter user name and password, then touch OK button to enter the User screen. -
Page 29: Change Password And Delete User
Operation manual of Thermal Cycler GET-S Code ZFD019 Figure 29. Login 4.7.2 Change Password and Delete User Only after login can passwords be changed and users be deleted, can other users be deleted from the login administrator account. -
Page 30: Chapter 5 Maintenance And Troubleshooting
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 5 Maintenance and Troubleshooting 5.1 Machine Maintenance 1). Regular cleaning A. Clean the holes on reaction block with neutral soap solution (Do not use any solvent containing strong alkali, strong alcohol and organic solution) B.Spaces near the left, right air vents and under the machine should be always kept free. -
Page 31: Notes
Operation manual of Thermal Cycler GET-S Code ZFD019 A. Incorrect or insufficient reactants. B. Denaturation temperature is too high or too low. It is suggested to set the temp. within range of 90-95℃ for 40 seconds. The duration can be adjusted in accordance with reaction volume. -
Page 32: Error Messages And Correspond Solutions
Operation manual of Thermal Cycler GET-S Code ZFD019 3). Note about cleaning Avoid any liquid getting inside the machine while cleaning the base of machine. Due to the possible usage of radioactive substance during experiment, please carefully handle cleaning.. It is not suitable to use the machine in moisture or hot environment. -
Page 33: Cause Of The Unnormal Phenomenon And Correspond Solution
Operation manual of Thermal Cycler GET-S Code ZFD019 Cause unnormal phenomenon correspond solution Description of problem Cause and correspond solution No any display after opening the Check whether plug inserted correctly and machine power output has electricity , pull out the plug... -
Page 34: Chapter 6 After-Sales Service
Operation manual of Thermal Cycler GET-S Code ZFD019 Chapter 6 After-sales Service 1. This product is warranted under normal usage to the original purchaser for two years from the date of purchase. 2. Lifetime service guarantee. The local dealer, maintenance station and regional offices will provide you excellent after-sales service. -
Page 35: Appendix 1 Warranty
Operation manual of Thermal Cycler GET-S Code ZFD019 Appendix 1 Warranty Origin Model User Name Telephone Address Postcode Name Postcode Address Telephone Vendor Vendor Seal Sale Date Invoice number Carry-in Repair Fault Cause of Disposition Produce for Repairer date tickets...
Need help?
Do you have a question about the GET-S and is the answer not in the manual?
Questions and answers