Page 1
Operating Manual BiO.Li.X! Storage Range Version: 1.0...
Page 2
No license is granted by acquisition of the product for any patent or patent rights of LiCONiC. If the user does not follow the instructions given in this manual, LiCONiC does not take any responsibility for injuries or damages caused by the LiCONiC product.
Page 3
History Page Document Change History Date Version Changes Author 23.11.2022 Initial Realease of updated Manual Ralf Kindle BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Table of Contents Contents About the Product and its Manual ................7 Intended Use ......................7 Unintended Use ......................7 Product Identification....................7 Scope of Delivery ......................7 General ........................8 Definitions ........................8 Target User Group ...................... 8 Importance of the Safety Instructions ................8 Labels .........................
Page 5
Kiwi Store Gassing and Backup Options ..............26 7.1.1 Safety Information......................26 7.1.2 Installation Safety Recommendations ................29 7.1.3 Backup Refrigeration installation ..................32 7.1.4 Specific Advice for LN2 Liquid Nitrogen................33 7.1.5 Refill LN2 Liquid Nitrogen cylinder ..................35 Alert System ......................36 FAB Alerts .........................
The LiCONiC BiOLiX! Store is intended for storage, identification and transfer of Microplates and Tubes. It is to be applied exclusively in the research field. The LiCONiC BiOLiX! Store is intended to work as a stand-alone unit or an element in an automated system.
General The LiCONiC BiOLiX! Store is a complete product for end users. Setup of equipment is conducted by LiCONiC Engineers or by LiCONiC trained and certified personnel. Definitions Operator: Any person who uses the equipment for its intended purpose. System Integrator:...
Labels Throughout this publication, Warning Notices are to be interpreted as follows: WARNING! Indicates the possibility of severe personal injury, loss of life or equipment damage if instructions are not followed. CAUTION! Indicates the possibility of severe equipment damage if instructions are not followed.
1.11 General Safety Regulations 1.11.1 Legal Requirements Please consult the Manufacturer's Declaration, delivered with each unit by LiCONiC AG, listing all applied directives and standards. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Only an authorized Engineer shall carry out inspection, maintenance and repair tasks. 1.11.3 Spare Parts to be used Only original LiCONiC Spare Parts may be used. If other parts are used during the normal warranty period, the manufacturer's guarantee may be invalidated. 1.11.4 Modifications Modifications shall only be carried out by an authorized Engineer.
General Biolix FAB Stores FAB Store 2.1.1 Store The Biolix FAB Store is the most compact storage with integrated cassette handling that covers fully-automated plate storage needs at –20°C and –80°C. The Biolix FAB Series offers dry climate storage. The modular mechanical design in combination with extremely simple commands eases the use of these systems in any environment.
2.1.2 Features All Biolix FAB Stores have compact dimensions. It has a user front door which allows comfortable and easy access to the ante chamber. The ante chamber is the operation space for the transfer of racks and plates from any additional equipment or transport box into the actual interface unit.
Page 14
Dryer Service Area Dryer / Cooler Ante Chamber Cooling Unit Cooling Units Ante Chamber BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 15
Service Access Automated Interface Manual Interface BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
The Systems are structured by their capacity in Microtiter plates (MTP) and their climate. The Biolix FAB Storages are Stand Alone System for fully-automated operation. For detailed systems dimensions please contact LiCONiC AG. These Systems are built to specification and specific system dimensions may vary according to system configuration.
Options Climate Overview Name Temperature Humidity DF Deep Freezer -20..00°C 02..45%RH ULT Ultra Low Temperature -80°C n.a. The DF Stores are designated for the use as compound storage, whereas the ULT version is mainly for Biobanking use. Cooling Within all units the air is actively dried by forcing the air through an evaporator. This allows cooling and actively drying the air.
Humidity All units are equipped with an RH-Sensor. This allows displaying the humidity inside the storage chamber. A very high-quality sensor is used for this purpose. Quality and accuracy of the sensor is maintained by the Swiss Federal Office of Metrology and the Swiss Accreditation Service.
The Kiwi units are distinguished through the capability to hold a tub handling unit under climatized conditions. The tube Handling is able to handle most tube types. The tube handling is integrated into the Liconic System and allows it to work under control climate conditions.
SBS 138 SBS 48 SBS 60 The use of HD formats on existing devices is possible but should be confirmed with a Liconic representative. Software configuration changes and teaching is required in any case. 4.2.2 Revolving Head Option This option allows the tube handling to hold two endeffectors for different tube formats.
4.2.5 Custom Tube Handlings For specific application custom solutions may be offered. These custom solutions will build on the mentioned features or a combination of them. Please contact Liconic for further information. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Identification Barcode reader A Bar Code Reader is installed on the Lift Assembly. It allows the reading of Bar Codes on plates stored in the cassettes. Bar Codes have to be printed on the shorter side of the plates and have to face outwards to the Lift Assembly. Cassettes Lift Assembly...
Falcon Eye FE2D-138 scanner A 2D code whole rack scanner can be integrated into the systems. It allows the reading of 2D Codes of a whole rack at once under controlled climate condition. To read the 2D codes, the rack needs moved as a whole to the reader. 5.2.1 Supported 2D code Types The optional 2D code reader recognizes the following 2D code types.
Series. The System Capacity depends on the specific configuration. The Kiwi Store can handle almost every plate or tube racks in SBS format currently available on the market. For optimum performance LiCONiC offers a variety of cassettes. BiO.Li.X! FAB V1.0...
Page 25
Store Cassette Example BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Installations Gassing Installation Kiwi Store Gassing and Backup Options The Kiwi Store series supports a wide selection of gassing and backup options. Typical properties of some commonly used options are described next. There are other gassing options available. Note that each gassing and backup option is linked to specific risks and hazards.
Page 27
WARNING ! ➢ In their liquid state inert gases are extremely cold fluids which can cause serious burns when in contact with the skin ➢ A cryogenic spill often gives a low-lying cloud of vapor that creeps along the ground When a cryogenic liquid is released into the atmosphere, it evaporates and forms a dense cloud.
Page 28
General Safety Instructions When working with inert gases prevent contact of liquid gas, cold vapors, or "snow" with exposed skin. Prevent entrapment of liquid in closed systems. Use only in well ventilated areas. Compressed gas cylinders contain gaseous and liquid gas at extremely high pressure and should be handled with care.
7.1.2 Installation Safety Recommendations Preventive Measures The very first preventive measure is to inform all persons who handle or who use inert gases: • Of the hazard represented by the refraction of oxygen in atmosphere • Of the preventive means to be employed in handling the gases •...
Page 30
Testing of Oxygen or Gas Content At present, various types of oxygen analyzers are available, which are often simple and reliable to operate. Such a monitor should at least have • A readout of the actual gas concentration • A settable alarm threshold value •...
Page 31
Personnel Protection Depending on the type of work to be performed, and the layout of the premises, the decision to provide additional protection for personnel may be taken on the initiative of the person in charge. Such additional protection may involve: •...
7.1.3 Backup Refrigeration installation On installations preferably choose pressurized liquid gas storage cylinders rather than wall outlets. The use of cylinders will limit the amount of gas being spilled in case of fatal failure. Whenever a wall outlet installation is chosen, make sure that there is a remote shut off valve installed outside the room where the liquid is used.
7.1.4 Specific Advice for LN2 Liquid Nitrogen Properties of Nitrogen Nitrogen is colorless, odorless and tasteless, as gas or liquid. It is nontoxic and non-flammable at atmospheric temperatures and pressures. A specific gravity of 0.9669 makes nitrogen slightly lighter than air. Nitrogen is mainly found in the atmosphere, where it accounts for 78.1% by volume of the air we breathe.
Page 34
In case of an accident evacuate all personnel from the affected area. Persons suffering from lack of oxygen should be removed to fresh air. If victim is not breathing, use trained personnel to administer a supplemental oxygen supply and artificial respiration to support vital functions. If breathing is difficult, administer oxygen.
7.1.5 Refill LN2 Liquid Nitrogen cylinder Whenever working with liquid Nitrogen use of personal safety equipment is required. Thermal gloves and protective glasses have to be worn at least. Refill interval of LN2 cylinder is depending on the required minimum backup time and the available LN2 cylinder volume.
Manual Operation Alert System The Biolix FAB Hybrid has a combined acoustic / visual alert system. An alert occurs when one or more abnormal statuses occur or when interaction is required. When the system is operating properly there is no alert. The various alerts are distinguished by the alert signal duration and the pause between the signals.
Barcode Reader 1D Reader for racks and plates The Barcode reader for plate/rack identification is mounted onto the handling mechanism. The Barcodes must be placed on the shorter side of the plates and must face towards the open side of the cassette. The barcodes should be parallel to the plate’s lower edge.
Cassettes 10.1 Interface Cassettes Cassettes allow simple and comfortable loading and unloading of storage samples and can be prepared outside the climate chamber. Once prepared, the samples can be quickly transferred into the climate chamber of the Kiwi unit. There is a wide variety of cassette types available. Make sure that you work with the proper cassette size that matches the type of plates you are using.
Page 39
The carousel must be kept clean as particles may cause the cassette to tilt. Do not place cassettes on the floor as they may pick-up dust etc. Always store cassettes inside the climate chamber or on clean surfaces. Use the cassette handle for transport of the cassettes. When lifting the cassette by its handle it will tilt backward slightly in order to prevent plates from shifting to the front.
Page 40
Cassette Handle Cassette Cassette Back Carousel The cassettes are secured by alignment rails to the carousel. The cassettes may be tilted slightly backwards (towards the front opening) for removal. In order to load a cassette align the back of the stacker base plate to the cassette alignment rail. Push the cassette forward until it stands vertically.
Page 41
CAUTION ! ➢ Make sure that ALL plates are pushed to the back of the cassette. Plates jutting out the front side of the cassette may cause collision with the handler shovel. ➢ Lift cassettes by their cassette handles only ➢...
10.3 FAB Store Cassettes Customized FAB cassettes allow simple and comfortable loading and unloading of storage samples. Once prepared, the samples can be quickly transferred into the climate chamber of the FAB unit by means of the cassette lifts installed. There is a wide variety of cassette types available.
Page 43
Cassettes must be treated with great care. They are aligned within 1mm when leaving the factory. Misaligned cassettes must be returned for re-alignment. Misaligned, tilted or bent cassettes may cause handling failures. Special care must be taken when cassettes are fully loaded with plates. Never apply force to the cassettes.
Page 44
CAUTION ! ➢ Make sure that ALL plates are pushed to the back of the cassette. Plates jutting out the front side of the cassette may cause collision with the cassette lift. ➢ Lift cassettes by their cassette handles only ➢...
Power-On Interface The instruments power is activated by the main switch. The instrument is turned on by pressing the power switch located at the front of the instrument. After power- on the climate portion of the instrument is activated automatically. An alarm will sound.
Power-On FAB Store The instruments power is activated by the main switch. The instrument is turned on by pressing the power switch located at the front of the instrument. The climate portion of the instrument is activated Independently. An alarm will sound. If the green light of the power switch does not turn on, check the fuse in the mains supply.
Page 47
To switch on the cooling unit the main power switch needs to be turned into the position stating ON. The key switch has to be in automatic position (see Picture 1). This has to be performed on both cooling units main and auxiliary. Status LED Key switch Main power switch...
Pressure CAUTION ! ➢ Do not disconnect any cables, except a LiCONiC engineer did advise to do ➢ All cooling units have to be permanently switched on. ➢ The key switch has to stay in automatic position, for normal operation.
CAUTION ! ➢ Ensure normal conditions being restored to operate the main cooling ➢ Ask for assistance from a LiCONiC engineer in case of insecurity about normal conditions and performing the recovery procedure. ➢ Observe cooling after recovery. Contact immediately a LiCONiC engineer in case of repeated stop of cooling after recovery.
Climate Settings 14.1 Temperature Settings System Temperature is pre-set. Temperature should only be changed by a qualified system administrator or LiCONiC engineer. 14.2 Humidity Settings Humidity is kept as low as possible and is not adjustable. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Routine Safety Checks Carefully read all safety instructions before operating the instrument. Be sure that you fully understand their content. Although the KIWI STORE is intended to be operated as a remote-controlled system, there still are some manual interactions required for operation. Apart from these operations there are also some very important safety checks the operator should be familiar with.
In an integrated environment such as a connection to a LIMS system the pick orders are sent through a direct connection to the Liconic System. These files are only used in a stand alone configuration.
Import – Export Interface 16.2 The Liconic Import and Export interface is used to create and execute operations with the result to load samples to the system or unload them from the system. In stand-alone configuration this can be used to introduce samples to the system, start pick orders and unload samples from the system.
Page 55
Picture 6: Import View with Rack details Selecting the import job, provides a detailed view of additional information in the “Properties” view (See Picture 6: Import View with Rack details). Information such as barcode, lab ware type and target storage partition are displayed. By right clicking on the import job it is possible to delete a created job.
Page 56
Picture 8: Detail Information on Task 1 By selecting an individual task of an import job additional information and properties are shown. The shown information depends on the current state each task is in. Picture 9: Detail Information on Task 1 In the “History”...
Page 57
Picture 10: Tools Menu Import Picture 11: Import Plate Dialogue The import dialogue will require to enter a barcode of the plate going to be loaded to the system. This is required for the system to recognize the plate and associate the given information to it.
Page 58
Chose the check box “Scan 2D” to include a 2D scanning step in the import. Should there be content provided for the plate, the scan step will compare the provided content with the physical present content. In case of a discrepancy the plate will be stored in a suitable safe area.
Page 59
Trigger Import To trigger an import either the door icon or the transfer station icon has to be pressed. The door icon will show a dialogue and guide through the steps of loading samples through the user door into the buffer cassettes. Picture 12: Icons to trigger Import The dialogue will tell when you need to open the user door.
16.2.2 Export The export view provides an overview of all registered jobs for collecting individual samples and plates to be unloaded. One export job can consist of several tasks. The application allows to create export jobs. Create Export Selecting in the Tools menu the entry “Pick Job” will open up the Windows file dialogue.
Page 61
Picture 15: Export Task Detail 1 If the actual pick task is selected the detail view varies according the state the pick task is in. In the created state only the list of tubes is shown. During the running state tubes vanish according them to be moved within the progress of the pick task. In the done state all tubes are listed with their target position and the status.
Page 62
Picture 17: Pick Task Management By right clicking onto the pick task the context menu is provided. This menu is active in the states created, running and error. The “Retry” and the “Continue” functions are solely used in the error status. Is the error state caused by a hardware error this needs to be resolved in order to continue operating the system.
Picture 19: Unload plate from system The second possibility requires to select in the context menu the item “Export Rack to Buffer” the plate will be moved into the buffer area inside the interface unit. To remove the rack the door icon in the interface has to be clicked. Follow the dialogues.
The plate is transferred directly to the transfer station if “Export” is selected. 16.2.4 Connections In the connection menu the connection to the scheduler can be reestablished. Also the “Update content” can manually be triggered. This function is used if an expected entry in the Import or Export view is not present.
16.3.1 General Functionalities In this chapter the functionality accessible to the administrator and the normal user are described. There is only one difference between user and administrator. Whilst the user has only access to the assigned area, the administrator has access to the full store.
16.3.2 Layout of the PlateExplorer The main window of the PlateExplorer is subdivided into the Menu bar, the contents tree on the left and the detailed information view on the right (see Picture 25). Picture 25: Main screen of the PlateExplorer BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 67
The Menu Bar The menu bar of the PlateExplorer covers four main entries. The “File”-menu item offers the user to “Exit” the program. Picture 26: File Menu Entry The “View” options enable the user to define the way to look at the items being displayed in the PlateExplorer’s Detail View on the right.
Page 68
The “Service” allows access to the Service functions. Currently it contains only the entry to reestablish the connection to the server. Picture 28: Service Menu Entry (to be checked) The “About” window will show information on the running PlateExplorer software. Picture 29: About Menu Entry BiO.Li.X! FAB V1.0...
Page 69
Tree View The tree view is located on the left side of the PlateExplorer window. All accessible positions plates are held are represented in the tree view. Transfer positions such as internal upper and bottom position, 2D Reader, Tube handling source/target and cassette positions can be accessed.
Page 70
Picture 31: Tree View Overview The Detail Information Screen The detail information screen on the right of the PlateExplorer shows the user all properties of the item selected within the tree view on the left. Its functionality can mostly be accessed with the help of the right-click action menus. The Partition management will be described in the corresponding chapter of this manual.
Page 71
Picture 32: Plate Explorer Detail View BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 72
Alternative to the detail view a location view can be selected. It is only available on plate level. A visualization of the plate with its physical positions is presented. The positions are represented as grey if they are empty or as green if a tube is present. A red position with a question mark indicates a tube present with an unreadable 2D code.
16.3.3 User Management and Alarm Notification The Administrator mode allows the creation, modification and deletion of users and their rights. In the picture below (Picture 34) the “User Manager” is shown. All users accessing to the system have to be created with this tool, even users accessing only through the Web-Access.
Page 74
To edit a user the desired user is selected from the list on the right and the “Edit” button pressed. The fields on the left side will get editable. Change the information and press the “Save” button to store the changes made. When finished, press “Close”...
16.3.4 Partitions Tool Depending on the use of the system different partitions can be created. Partitions are virtual subdivisions and can be used to split the storage capacity for different uses, user groups or experiments. To launch the partition tool mark the system unit with a left click, then right click and select “Partition”...
Page 76
Picture 37: Right Click Menu Graphic Picture 38: Right Click Menu Table Existing partitions can easily be manipulated. Just right click into the graphic or the table and select the option. The “Add Partition” in the graphic is only available if unassigned cassettes (represented in grey) are available.
Page 78
Unassigned cassettes can be used to create a new partition (Picture 41) or assign them to an existing partition (Picture 42). Adding cassettes to existing partition will be represented by the same coloring. In the table the cassettes will be displayed in order only the partition name will show their association.
Page 79
Picture 43: Dialogue Rename If cassettes are “scattered” meaning a partition consists of cassettes not being next to each other the partition segments have to be deleted individually (Picture 44). Picture 44: Deleted Partition A BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 80
Also partitions may be resized (Picture 45). The partitions may be made larger if unassigned cassettes are available or made smaller if they consist of more than one cassette. Resizing is not available if the minimal partition size of one cassette is present and no unassigned cassettes next to the partition available (Picture 46).
16.3.5 Right-Click Action Menus in the Tree View The right-click action menus are the most powerful tool within the PlateExplorer. They cover most of the commands and their content is depending on where they get activated. Right-Click a “Unit” When right-clicking a unit, a sub menu appears as shown in Picture 47 below. Picture 47: Right Click Menu Unit “Initialize”...
Page 82
Picture 48: Unit properties Right-Click a “Partition” When right-clicking a partition, a sub menu with several options appears. Picture 49: Right Click Menu Partition “Inventory” starts a 1D barcode inventory on the selected partition. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 83
“Search” lets the user search within the partition for key words such a plate barcode, tube code or fragments of them. Additional search parameters are available. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 84
Right-Click a “Cassette” When right-clicking a cassette, there are two options to choose from: Picture 50: Right Click Menu Cassette “Properties” will show the usage graph of the clicked cassette and a check box to block the complete cassette for administrative reasons. BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
Page 85
Right-Click a “Level” When right-clicking a level, several options will appear: Picture 51: Right Click Menu Level (to be checked) “Cut” and “Paste” will enable the user to move plates within the system as described in 1.3.1. under “Using Cut&Paste”. “Load”...
Page 86
The “Properties” of a rack in a level allows under the tab “Plate” to set the PlateID, add some note, see location information of the chosen Level. Furthermore, the plate type can be removed or the plate type changed. Changes of the plate type are only possible with certain limitations.
Page 87
Under the tab “Tubes” it is possible to set the tube type if not yet set and load content from an XML file. Furthermore, the content of the plate can be saved as a file. Some general information about the tube configuration and the content of the plate is shown such as the tube parameters, number of tubes and free locations.
16.3.6 History The “History” tab in the level and plate properties allows to access the history data for the respective. The history contains the information which user did conduct, the respective Event at which date and what Barcode and User ID was involved. Picture 54: Properties History Level If no plate is present when selecting a level only the history of the respective level is available.
Page 89
Is a plate present on the selected level, the history of this plate is available. Similar to the level history the User manipulating the plate, the date, the performed Event and the location of the plate are displayed. Picture 55: Properties History Plate BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
16.3.7 Right-Click Menu on Tubes at Tube Handling The right-click menu on the tube handling unit covers the following items: “Cut” and “Paste” enable the user to manually move tubes from plate to plate on the device. “Check Presence” will work only depending on the model of the tube handling. If integrated, the function checks if a tube exists on the clicked location within the rack.
16.3.8 Right-Click Action Menu on 2D Rack Reader The right-click menu on the 2D Rack Reader allows to select the following items: “Cut” and “Paste” enable the user to manually move plates and racks from and to the reader. “Check Presence” will check if a plate or rack is present on the reader. “Property”...
Page 93
Selecting the “Scan” option will trigger a scanning process when a plate is present on the scanner. An additional Window is opened showing the result of the scan. This window allows to re-scan or safe the scan results into the database. It has two display options one shows the locations of the tubes in a visual representation and the other one as a list.
16.4 Job Manager The JobManager Web based interface allows to manipulate jobs. The different coloring of the jobs are indicating their status and manipulation can be performed upon them such as Cancel, Continue, Retry, Delete. The available functions are provided by each job/task. If a job/task is in error it will offer the options Continue, Retry and Cancel if they are possible.
Page 95
Picture 62: JobManager Main Page Jobs in the different states allow for different manipulations. To delete a job from the queue it has to be first cancelled. The cancelation of a job can be easily performed by pressing the “X” within the circle at the right of each job. Only after the cancelation the delete icon in the shape of a trash bin becomes available.
16.5 Climate Reporting The climate reporting interface is used to extract climate data from the database. The historic and current climate values can be accessed, displayed and saved for further use. To access the Climate Reporting user interface, it is required to log in. The access to this may be restricted, depending on the user access rights Picture 65: Climate Reporting Log In The main page after the log in will show an overview of all device which have...
Page 98
To access the detail view for a device, the device is selected and clicked. Afterward a graph view is presented. Picture 67: Climate Reporting Device Selection The graph view allows to view current and historic data. The displayed data can be selected by device.
Page 99
From the graph view it is possible to extract the climate data. The graphs can be saved as picture or directly printed. The climate data can also be exported as a CSV file for further use. Picture 69: Climate Reporting Save BiO.Li.X! FAB V1.0 RKi, 23.11.2022...
This terminal should only be used by trained personnel or under the supervision of a Liconic engineer. If there are any uncertainty in the use of the terminal please contact your local engineer or the Liconic service organization.
Technical Data Technical Data 18.1 Mechanics • Cassettes System Configuration Dependant • Levels Depending on used lab ware • Position drives Stepper Motor, bipolar, micro-stepping mode • Shovel drive DC-Motor • Noise <82dBA / 1m • RAL Color 9010 18.2 Electronics 18.2.1 Robotics...
Page 102
For detailed installation requirements refer to the section installation. 18.6 Overall Dimensions System dimensions do depend on the configuration. Dimension for specific systems may be retrieved from LiCONiC according to the needs and room constraints. 18.7 Weights System weights do depend on the configuration. Weights for specific systems may be retrieved from LiCONiC according to the needs and room constraints.
Need help?
Do you have a question about the BiO.Li.X! FAB and is the answer not in the manual?
Questions and answers