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bNovate Technologies BactoSense Multi Reference Handbook

Rapid bacterial monitoring system
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Document number: 40203-01-EN
Version: 1
Valid from: SW 2.1
REFERENCE HANDBOOK
BactoSense Multi
Rapid bacterial monitoring system

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  • Page 1 Document number: 40203-01-EN Version: 1 Valid from: SW 2.1 REFERENCE HANDBOOK BactoSense Multi Rapid bacterial monitoring system...
  • Page 2 © bNovate Technologies SA, subject to technical changes without notice 4/2023 This document has been written by bNovate Technologies SA. Copying or modifying the content or giving this document to third parties is permitted only with the expressed consent of bNovate Technologies SA.

  • Page 3: Table Of Contents

    Reference Handbook BactoSense Table of Contents 1 General user information ........1 1.1 Purpose of the reference manual .

  • Page 4: General User Information

    The reference manual is intended for all persons who are familiar with the contents of the instruction manual and require detailed information about subjects such as design, configuration and repairs. The intented use of the BactoSense Multi is described in 40202 Instruction Manual BSM.

  • Page 5: Pictograms

    General user information Reference Handbook BactoSense Injury or hazards to health with long-term effects. Ignoring this warning may lead to injuries with possible long-term effects. WARNING! Material damage. Ignoring this notice may cause material damage to the instrument and its peripherals. CAUTION! Pictograms pictograms...

  • Page 6: Measurements

    Reference Handbook BactoSense Measurements Measurements Measurement result This page shows the result of the latest measurement. From here, the user can delete a measurement, look up older results (and export them), or export this result. Expert users can repeat the cell counting with new gates. Some actions are bound to specific accounts (Basic, Advanced or Admin).

  • Page 7: Measurement Parameters

    Measurements Reference Handbook BactoSense Measurement parameters 2.2.1 TCC cartridge With a TCC cartridge, the following parameters are displayed: PARAMETER UNIT NAME DESCRIPTION 1/ml Total Cell Total number of bacteria detected inside the Count TCC gate. It is an addition of HNAC and LNAC: HNAP High Nucleic...
  • Page 8 Reference Handbook BactoSense Measurements Gate: a line or polygon delimited on the dotplots. • • Set of Gates: a collection of two or more gates, used for the gating strategy. The types of gates are fixed, but users can change the limits of each gate. These are different for TCC and ICC measurements.
  • Page 9 Measurements Reference Handbook BactoSense The BactoSense knows four types of gate sets: • TCC, used with the TCC cartridge. • ICC, used with the ICC cartridge. • FIT, used only for beads validation protocols, with any cartridge. gate sets contain two gates: TCC: a polygon defined on the FL1-FL2 plane.

  • Page 10: Tcc / Icc Gates

    Reference Handbook BactoSense Measurements 2.3.1 TCC / ICC Gates BactoSense, when using a TCC (respectively ICC) cartridge, defines two gates to count cells. The first gate, TCC for Total Cell Count (respectively ICC for Intact Cell Count), is a polygon defined on the FL1 vs FL2 dotplot.

  • Page 11: Define The Gate

    Measurements Reference Handbook BactoSense The following procedure describes how to adjust the gates: WORK STEP ADDITIONAL INFO / IMAGE Press the Home button. Press the Back button as many times as needed for the Home button to appear. Press the Gate settings button.
  • Page 12 Reference Handbook BactoSense Measurements WORK STEP ADDITIONAL INFO / IMAGE Prepare your sample and start a Water Analysis protocol. On the Result details page, the FL1 vs FL2 dotplot shows: 1) Bacteria LNA and HNA in the water 2) Electrical noise from the optical detectors 3) Debris/background noise (These may include damaged cells, stating aggregates or particles)

  • Page 13: Comparison Of Measurement Scales

    Measurements Reference Handbook BactoSense WORK STEP ADDITIONAL INFO / IMAGE Locate the separation between noise and data: open the histogram and locate the first trough after the noise peak, i.e., the first minimum after the peak at the extreme left (arrow in the picture).
  • Page 14 Reference Handbook BactoSense Measurements WORK STEP ADDITIONAL INFO / IMAGE It is recommended to use the Validation Kit Read the instructions of the which contains ready to use beads solutions. Validation Kit for more information. Install the manual sampling device according to the Instruction Manual.
  • Page 15 Measurements Reference Handbook BactoSense WORK STEP ADDITIONAL INFO / IMAGE On the FL1 vs counts histogram, estimate the log value of each peak’s center on the FL1 axis. In this example, we obtain approximately (in log): peak = 4.05 peak = 4.3 peak = 5.35 peak = 6.2 Measure the same beads with the other flow...

  • Page 16: Export Data

    Reference Handbook BactoSense Measurements Export data 2.6.1 Export single measurement The export function saves data to an USB flash drive or hard drive. It creates a folder for the selected measurement, which contain its FCS file and plots in PNG format. Additionally, CSV and Excel files are generated, containing a summary of this measurement result (TCC, ICC, HNAC, LNAC, HNAP).

  • Page 17: Export Measurement Series

    Measurements Reference Handbook BactoSense 2.6.2 Export measurement series The export function saves data to a USB flash drive or hard drive. It creates one folder per measurement, which contain the FCS file and plots in PNG format. Additionally, CSV and Excel files are generated, containing a chronological list of all measurement results (TCC, HNAC, LNAC, HNAP, ICC).

  • Page 18: Export Selected Measurements

    Reference Handbook BactoSense Measurements 2.6.3 Export selected measurements The export function saves data to an USB flash drive or hard drive. It creates a folder for the selected measurement, which contain its FCS file and plots in PNG format. Additionally, CSV and Excel files are generated, containing a summary of this measurement result (TCC, ICC, HNAC, LNAC, HNAP).

  • Page 19: Export All Data Or Diagnostic Data

    Measurements Reference Handbook BactoSense 2.6.4 Export all data or diagnostic data The “Special import/export” function allows users to export either all measurement data from the instrument, or to export only diagnostics data which can be interpreted by service technicians. WORK STEP ADDITIONAL INFO / IMAGE Press the Home...

  • Page 20: Manage Services With Ftp

    Reference Handbook BactoSense Measurements 2.6.5 Manage services with FTP Data can be remotely accessed using the FTP protocol (RFC 3659). Authenticate with the same users and passwords as the authentication on the instrument itself or on the web user interface.  ...

  • Page 21: Settings

    Settings Reference Handbook BactoSense Settings Demo mode Demo mode can be used when demonstrating the device or when learning how to use the menus. When it is activated, the instrument reboots into a special mode that displays demonstration data (the owner’s measurement results are hidden). All the instrument’s functions are replaced by simulations that never move the hardware or modify the results database.

  • Page 22: Network Configuration

    Reference Handbook BactoSense Settings The following procedure describes how to perform a factory reset: WORK STEP ADDITIONAL INFO / IMAGE Press the Home button. Press the Back button as many times as needed for the Home button to appear. Enter the System settings then Factory...

  • Page 23: Set Ntp Servers

    Settings Reference Handbook BactoSense Set NTP Servers NTP is a computer network protocol which is used to synchronise time on computers across a network. By default, these are set to synchronise to the Network Time Foundation servers (ntp.org). You have the possibility to set custom NTP servers in the Time menu. WORK STEP ADDITIONAL INFO / IMAGE Press the...

  • Page 24: Service Information

    Reference Handbook BactoSense Settings Service information Figure 3-1 Intervention info screen WORK STEP ADDITIONAL INFO / IMAGE Press the Home button. Press the Back button as many times as needed for the Home button to appear. Press the Maintenance button. Press the Intervention info button.

  • Page 25: Service Interventions Log

    Settings Reference Handbook BactoSense Service interventions log The following procedure describes how to view details of past service interventions. WORK STEP ADDITIONAL INFO / IMAGE Press the Home button. Press the Back button as many times as needed for the Home button to appear.
  • Page 26 Reference Handbook BactoSense Settings WORK STEP ADDITIONAL INFO / IMAGE Press Import settings Select the following settings you want to import. Measurement settings include gate sets, IO settings, alarms, and date and time settings. Network settings comprise IP, DNS, and NTP addresses Press Import Settings and wait until the device reboots...

  • Page 27: Web User Interface

    Web user interface Reference Handbook BactoSense Web user interface General information The web interface allows users to browse and export results from a remote computer,  as well as monitor the state of the instrument (cartridge level, errors, etc.) The BactoSense needs to be on the same network as the computer that accesses the ...

  • Page 28: Start Page In Manual Mode

    Reference Handbook BactoSense Web user interface Start page in Manual mode Figure 4-1 Start page on web user interface   Menu State of the device Language: Drop-down menu for   Latest results changing the language. Progress of the current ...

  • Page 29: Download A Zip File With Multiple Results

    Web user interface Reference Handbook BactoSense Download a zip file with multiple results The results from multiple measurements can be downloaded as a zip file. This zip includes the FCS file and summary plots of each measurement. WORK STEP ADDITIONAL INFO / IMAGES Go to the page Results.

  • Page 30: Animate The Evolution Of Dot Plots

    Reference Handbook BactoSense Web user interface Animate the evolution of dot plots The web interface can animate dot plots from the Auto mode, to help visualize the progression of the measurements. This feature shows static images in rapid succession; therefore the animation can only be exported using screen recording software. WORK STEP ADDITIONAL INFO / IMAGES Go to the page Dotplots.

  • Page 31: Take Screenshots Of The Touch Gui

    Web user interface Reference Handbook BactoSense Take screenshots of the touch GUI Take screenshots of the touch GUI from the web interface: WORK STEP ADDITIONAL INFO / IMAGES Press the System info button. Press Screenshot. The screenshot is displayed below. 4.10 Power off and reboot from web GUI The device can be switched off or rebooted from the web interface according to the followed procedure:...

  • Page 32: Error Messages And Troubleshooting

    Reference Handbook BactoSense Error messages and troubleshooting Error messages and troubleshooting Warnings Warnings appear when unusual behavior is detected during an otherwise successful measurement. They can indicate reduced accuracy of the measurement results or indicate impending errors. Unlike errors, warnings do not prevent the instrument from functioning, but operators should pay attention to them as they can indicate sources of inaccuracies.
  • Page 33 Error messages and troubleshooting Reference Handbook BactoSense ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE Air bubbles The sample appears to • Check sampling device is detected contain air bubbles. installed properly. • Check the sample for bubbles. • If using online sampling device, check water is supplied at correct...

  • Page 34: Non-Critical Error Messages

    Reference Handbook BactoSense Error messages and troubleshooting Non-critical error messages Non-critical errors prevent a measurement from terminating successfully, but do not prevent the instrument from running another measurement afterwards. These errors do not require human intervention. Some non-critical errors are promoted to critical errors if they repeat three times.
  • Page 35 Error messages and troubleshooting Reference Handbook BactoSense ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE Mixer inflation Underpressure detected • Missing sample. error during mix or dispense. • Air in the input. • Leak in the system. • Please execute Clean Optics protocol.
  • Page 36 Reference Handbook BactoSense Error messages and troubleshooting ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE GPIO Timeout The processing board is • Non-critical error. error waiting for another Repeat the module to respond. measurement. If the problem persists, contact a service technician.

  • Page 37: Critical Errors

    Error messages and troubleshooting Reference Handbook BactoSense Critical Errors CRITICAL ERRORS If a critical error occurs during operation, it has the following effects: • The protocol immediately stops. • The instrument goes into critical error state, and manual intervention is needed before any new protocol can be launched.
  • Page 38 Reference Handbook BactoSense Error messages and troubleshooting ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE FPGA Failed to initialize the • Reboot the initialization signal processing chip. instrument. error Laser end of life Laser has reached end of • Service required.
  • Page 39 Error messages and troubleshooting Reference Handbook BactoSense ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE Empty dye Dye tube connected to • The tube is supply cartridge is empty. disconnected. • Cartridge is defective. • Repeat the cartridge change procedure, making sure that all tubes are properly connected.
  • Page 40 Reference Handbook BactoSense Error messages and troubleshooting ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE No Tray No vial detected • Check that the tray Detected Error and vials are installed in the autosampler. • Place a tray in the autosampler, then clear the error and retry.

  • Page 41: Low Temperature Operation And Standby Heating

    Error messages and troubleshooting Reference Handbook BactoSense ERROR NAME DESCRIPTION CAUSES / WHAT TO DO CODE External Ambient temperature is • Increase ambient temperature too outside of the temperature. instrument • Trying to operate or specifications. store the instrument below 5 °C can cause damage to the device.

  • Page 42: Table Of Acronyms

    Reference Handbook BactoSense Table of acronyms Table of acronyms Common acronyms in flow cytometry are listed below Table 6-1 Acronyms NAME OF DESCRIPTION ACRONYM Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments. The FCS specification has traditionally been developed and maintained by the International Society for Advancement of Cytometry (ISAC).

  • Page 43: Index

    Index Instruction Manual BactoSense Index Export 13 Interval 27 Adjust gates 4 Parameters 4 Alarm configuration Results 3 Gate 18 Measurement parameters ICC 4 Configuration TCC 4 Network 19 Critical errors 34 Network configuration 19 CSV 26 Non-critical errors 31 NTP servers 20 Demo mode 18 Operation...
  • Page 44 Technologies SA Tel. +41 (0)21 552 14 21 Ch. Dent d’Oche 1A info@bnovate.com CH-1024 Ecublens www.bnovate.com Switzerland...

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