Related Manuals for Omega Bio-Tek Mag-Bind RXNPure Plus
Summary of Contents for Omega Bio-Tek Mag-Bind RXNPure Plus
- Page 1 Mag-Bind® RXNPure Plus M1386-01 50 mL Manual Date: February 2023 Revision Number: v4.0...
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Page 3: Table Of Contents
Mag-Bind® RXNPure Plus Table of Contents Introduction ..................2 Illustrated Protocol................3 Kit Contents/Preparations/ Storage and Stability....4 Mag-Bind® RXNPure Plus 96-well Plate Protocol....5 Mag-Bind® RXNPure Plus 384-well Plate Protocol....8 Troubleshooting Guide.............11 Manual Date: February 2023 Revision Number: v4.0... -
Page 4: Introduction
Introduction Omega Bio-tek’s Mag-Bind® RXNPure Plus Kit allows rapid and reliable isolation of PCR products with high recovery rates. The system combines Omega Bio-tek’s proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively binds PCR amplicons 100 bp and larger and eliminates excess nucleotides, primers, and small, non-targeted amplification products, such as primer dimers. -
Page 5: Illustrated Protocol
Illustrated Protocol Measure the PCR Reaction Add Mag-Bind® RXNPure Plus and Mix Magnetize and Remove Supernatant Wash Twice with 70% Ethanol Elute DNA... -
Page 6: Kit Contents/Preparations/ Storage And Stability
Kit Contents Product Number M1386-01 Mag-Bind® RXNPure Plus 50 mL User Manual Preparations PCR Reaction Volume M1386-01 96 well format 50 mL 10 μL 2,777 preps 25 μL 1,111 preps 50 μL 555 preps 100 μL 277 preps PCR Reaction Volume M1386-01 384 well format 50 mL... -
Page 7: Mag-Bind® Rxnpure Plus 96-Well Plate Protocol
Mag-Bind® RXNPure Plus 96-well Plate Protocol Important: If automating this procedure on a liquid handler or a magnetic processor, please contact your Omega Bio-tek representative for instrument-specific instructions. Materials and Equipment to be Supplied by User: • Vortexer • Magnetic Separation Device (Recommended Cat# AlpAqua A001322) •... - Page 8 Mag-Bind® RXNPure Plus Add 1.8 volumes Mag-Bind® RXNPure Plus. PCR Reaction Volume (μL) Mag-Bind® RXNPure Plus (μL) Pipet up and down 5-10 times or vortex for 30 seconds. Let sit for 5 minutes at room temperature. Place the plate on a magnetic separation device to magnetize the Mag-Bind® RXNPure Plus.
- Page 9 Mag-Bind® RXNPure Plus 13. Leave the plate on the magnetic separation device for 10-15 minutes to air dry the Mag-Bind® RXNPure Plus. Remove any residual liquid with a pipettor. Note: It is important to dry the Mag-Bind® RXNPure Plus before elution. Residual ethanol may interfere with downstream applications.
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Page 10: Mag-Bind® Rxnpure Plus 384-Well Plate Protocol
Mag-Bind® RXNPure Plus 384-well Plate Protocol Important: If automating this procedure on a liquid handler or a magnetic processor, please contact your Omega Bio-tek representative for instrument-specific instructions. Materials and Equipment to be Supplied by User: • Vortexer • Magnetic separation device for 384-well PCR plates •... - Page 11 Mag-Bind® RXNPure Plus Pipet up and down 5-10 times or vortex for 30 seconds. Let sit for 1 minute at room temperature. Place the plate on a magnetic separation device to magnetize the Mag-Bind® RXNPure Plus. Let sit at room temperature until the Mag-Bind® RXNPure Plus is completely cleared from solution.
- Page 12 Mag-Bind® RXNPure Plus 16. Pipet up and down 20 times or vortex for 30 seconds. 17. Let sit for 2-3 minutes at room temperature. 18. Place the plate on a magnetic separation device to magnetize the Mag-Bind® RXNPure Plus. Let sit at room temperature until the Mag-Bind® RXNPure Plus is completely cleared from solution.
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Page 13: Troubleshooting Guide
Troubleshooting Guide Please use this guide to troubleshoot any problems that may arise. For further assistance, please contact the technical support staff, toll free, at 800-832-8896. Possible Problems and Suggestions Problem Cause Solution Increase the number amplification cycles Low PCR product yield for PCR. - Page 14 Notes:...
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