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Summary of Contents for Omega Bio-Tek Mag-Bind SeqDTR M1300-05
- Page 1 Mag-Bind® SeqDTR™ M1300-05 5 mL M1300-08 50 mL M1300-50 500 mL January 2013...
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Page 3: Table Of Contents
Mag-Bind® SeqDTR™ Table of Contents Introduction and Overview............2 Illustrated Protocol................3 Kit Contents and Storage............4 Mag-Bind® SeqDTR™ 96-well Plate Protocol......5 Mag-Bind® SeqDTR™ 384-well Plate Protocol.....7 Troubleshooting Guide...............9 Ordering Information..............10 Manual Revision: January 2013... -
Page 4: Introduction And Overview
For optimal sequencing results, remaining labeled dideoxynucleotides should be removed prior to electrophoresis. Omega Bio-tek’s Mag-Bind® SeqDTR™ is designed to effectively and reliably remove unincorporated terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads to eliminate excess nucleotides, primers, and small, non-targeted amplification products such as primer dimers. -
Page 5: Illustrated Protocol
Illustrated Protocol Add Mag-Bind® SeqDTR™ and 85% Ethanol, Mix Magnetize and remove cleared supernatant Wash 2 x with 85% Ethanol Elute and transfer DNA to a new plate... -
Page 6: Kit Contents And Storage
Kit Contents and Storage Product No. M1300-05 M1300-08 M1300-50 Mag-Bind® SeqDTR™ 5 ml 50ml 500 ml Preparations 500* / 1,000** 5,000* / 10,000** 50,000 */ 100,000** User Manual * Based on a typical 10 μL reaction volume in a 96-well format ** Based on a typical 5 μL reaction volume in a 384-well format Storage and Stability Mag-Bind®... -
Page 7: Mag-Bind® Seqdtr™ 96-Well Plate Protocol
Mag-Bind® SeqDTR™ - 96-well Plate Protocol Mag-Bind® SeqDTR™ 96 Plate Format Protocol Materials and Equipment to be Supplied by User: • 85% ethanol (do not use denatured ethanol) • Magnetic separation device compatible with 96-well PCR plates • Multichannel pipet •... - Page 8 Mag-Bind® SeqDTR™ - 96-well Plate Protocol Add 100 μL 85% ethanol to each well. It is not necessary to resuspend the Mag-Bind® SeqDTR™. Let sit at room temperature until the Mag-Bind® SeqDTR™ is completely cleared from solution. Aspirate and discard the supernatant. Do not disturb the Mag-Bind® SeqDTR™. Repeat Steps 6-8 for a second 85% ethanol wash step.
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Page 9: Mag-Bind® Seqdtr™ 384-Well Plate Protocol
Mag-Bind® SeqDTR™ - 384-well Plate Protocol Mag-Bind® SeqDTR™ - 384-well Plate Protocol Additional Materials Supplied by User: • 85% ethanol (do not use denatured ethanol) • Magnetic separation device compatible with 384-well microplates • Multichannel pipet • Reservoirs • 384-well plate capable of being used in sequencers •... - Page 10 Mag-Bind® SeqDTR™ - 384-well Plate Protocol Let sit at room temperature until the Mag-Bind® SeqDTR™ is completely cleared from solution. Aspirate and discard the supernatant. Do not disturb the Mag-Bind® SeqDTR™. Repeat Steps 6-8 for a second 85% ethanol wash step. 10.
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Page 11: Troubleshooting Guide
Troubleshooting Guide Please use this guide to troubleshoot any problems that may arise. For further assistance, please contact the technical support staff , toll free, at 800-832-8896. Possible Problems and Suggestions Problem Cause Solution Supernatant is not Make sure to remove any liquid drops removed completely from each well of the plate. -
Page 12: Ordering Information
Mag-Bind® E-Z Pure (500 mL) M1380-02 Mag-Bind® and SeqDTR™ are trademarks of Omega Bio-tek, Inc. BigDye is a registered trademarks of Applied Biosystems or its subsidiaries in the U.S PCR is a patented process of Hoffman-La Roche. Use of the PCR process requires a license. - Page 13 Notes:...
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