Related Manuals for Columbus Instruments Rotamex-5
Summary of Contents for Columbus Instruments Rotamex-5
- Page 1 Rotamex-5 0254-0001 v1.11 August 14, 2023 950 North Hague Ave. Phone: +1.614.276.0861 Columbus, Ohio 43204-2121 USA Fax: +1.614.276.0529 www.colinst.com Toll Free: +1.800.669.5011...
- Page 2 Rotamex-5 0254-0001 v1.11...
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Page 3: Table Of Contents
Rotamex-5 0254-0001 v1.11 Table of Contents 1 – Introduction ........................................4 1.1System Overview ......................................4 1.2 System Specifications ....................................4 2 – Hardware Installation .....................................5 2.1 Changing Rod Assembly ...................................5 2.2 Mouse Rod Assembly ....................................6 2.3 Rat Rod Assembly ......................................6 2.4 Sensor Adjustment ....................................7 2.5 Detector and Sensor Cable Connection Guide ...........................8... -
Page 4: Introduction
Rotarod's current speed at the time of the subjects fall are recorded. All experiment setup parameters are also recorded. Rotamex-5 can be used as a stand-alone device or it can be interfaced through an RS232 communication port to Windows compatible computer for storing data for later review or importation into a third party statistical analysis program. -
Page 5: Hardware Installation
2 – Hardware Installation The Rotamex-5 comes pre-assembled from the factory with either a standard mouse or rat rod assembly. There is no need for additional assembly. The instrument should be placed on a flat study surface. If the optional lid assembly was purchased ensure that there is enough room for the lid assembly to open to allow easy placement of animals on the units rod assembly. -
Page 6: Mouse Rod Assembly
2.2 Mouse Rod Assembly The Rotamex-5 can be configured with a smaller rod assembly that has a 3.0 cm rod diameter. This is the preferred rod when running smaller animals such as mice. The width of the running surface is approximately 9.5 cm. The rod surface is constructed from grey PVC with a knurled finished. -
Page 7: Sensor Adjustment
2.4 Sensor Adjustment The Rotamex-5 incorporates a 32-beam optical sensor mounter slightly over the rotarod assembly. This approach allows for more precise detection of when the animal leaves or falls from the rod assembly. This method also allows for detection of the animal gripping the rod assembly and rotating along with the rod as it spins. -
Page 8: Detector And Sensor Cable Connection Guide
Rotamex-5 0254-0001 v1.11 2.5 Detector and Sensor Cable Connection Guide The following is a guide for connecting the RJ45 cables to the emitters and detectors to the front controller. Controller and Sensors The front detector (figure 1, item A) is located on the left side, and its associated emitter (figure 1, item B) is located directly across from it on the rear. - Page 9 Rotamex-5 0254-0001 v1.11 Sensor Cable Installation Both plug ends on each cable have an assigned number that matches the number assigned to a particular jack on the detectors, emitters, and controller. Cable installation is made simple by matching the numbers on the plugs with the numbers on the jacks.
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Page 10: Modified Sensor Bracket Setting Guide
The modified sensor brackets have additional settings for adjusting the height of the sensors for both mouse and rat configurations. As shown in the Rotamex-5 Instruction manual on page 8, the mouse position set the detection beam 1/4 inches above the rod. The bracket shown is the original bracket with five mounting holes. The holes are numbered from one through five with number one at the bottom hole. - Page 11 Rotamex-5 0254-0001 v1.11 Proper Mounting of the sensors The emitters and detectors must be installed with their window ports (figure 2, item A) in the upper position and the ID label facing up (figure 4). The backsides of the emitters and detectors have four threaded 6-32 mounting holes (figure 3) that are used to secure the sensors to the brackets.
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- Page 13 Adjust the sensors using the following procedure: Select the Diagnostics Menu on the controller (see page 15 of Rotamex-5 Instruction Manual). At the Diagnostics Menu shown on the LCD of the controller, the sensor portion of the display should show the numeric value of "00"...
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Page 14: Controller
3 – Controller The Rotamex-5 was designed to operate in a standalone mode where a host computer is not needed for normal operation. The main controller is located on the front of the unit. It contains four buttons which are used to enable each lane as well as... -
Page 15: Controller Hardware Connections
3.1 Controller Hardware Connections The Rotamex-5 Controller controls monitors the animals presence on the rod through two 16 beam optical sensors. It also controls the motor used to turn the rota-rod assembly. These hardware components are connected to the left side of the controller unit. -
Page 16: Controller Keypad
3.2 Controller Keypad The Rotamex-5 was designed to operate in a standalone mode where a host computer is not needed for normal operation. The main controller is located on the front of the unit. It contains four buttons which are used to enable each lane as well as an "UP", "DOWN", "ENTER"... -
Page 17: Startup Menu
Rotamex-5 0254-0001 v1.11 3.3 Startup Menu The Startup Menu of the Rotamex-5 is displayed immediately after power up. On this menu the firmware version number can be found. By pressing any key, the Main Menu will be displayed. 3.4 Main Menu The Main Menu enables access to functions to edit experiment variables, run experiments, and diagnostics functions. -
Page 18: Experiment Setup Menu
Rotamex-5 0254-0001 v1.11 Experiment Setup Menu. This menu is used to setup rod Start Speed, End Speed, Acceleration Speed and Acceleration period. Run Experiment Menu. This menu selections displays the experiment screen. Diagnostics Menu. This menu selection allows the user to monitor in real time the optical sensors as well as control of the Rotamex motor. -
Page 19: Run Experiment
Rotamex-5 0254-0001 v1.11 3.6 Run Experiment The Run Experiment Menu allows experiments to be run. From this menu the user can enable channels and start / stop experiments. When the experiment is running the current speed of the rota-rod assembly is displayed, channels running as well as the amount of elapsed time since the experiment has started. -
Page 20: Diagnostics
Rotamex-5 0254-0001 v1.11 3.7 Diagnostics The Diagnostics allows the user to monitor the optical sensors as well control the rota-rod's motor. The motor speed (RPM) can be increased and decreased by pressing the "UP" and "Down" button. In the sensor portion of the diagnostics each beam position that is broken is displayed as its binary beam position. -
Page 21: Software
The interface uses a 9 pin D-sub connector for the computer RS-232 plug (figure 1), and a dual RJ-45 jack for connecting the Rotamex-5 controller (figure 2). A RS-232 9 pin to 9 pin D-sub cable (figure 3) is needed to connect the... -
Page 22: Running Software
RJ-45 "Computer" jacks on the Rotamex-5 controller. 4.3 Running Software The Rotamex-5 Interface software can be run clicking on the Rotamex-5 Interface folder located in the Programs Menu. The Rotamex-5 Interface splash screen will be shown. -
Page 23: Communication Port Setup
If no devices are found on the communication ports the user will have to manually select a device to search the communication ports for. If the system is configured with one Rotamex-5, then the stating address of 128 is used. If there are additional Rotamex-5 present with the system, then their addresses will follow 128. -
Page 24: Menus
Rotamex-5 0254-0001 v1.11 4.4 Menus The Rotamex-5 software contains many utilities to run experiments, as well as test and troubleshoot the hardware devices configured to the system. The Utilities can be access from the main menu. Experiment Menu. The Experiment Menu contains utilities to create new experiments and run protocols from previously performed experiments. - Page 25 In the top of the Experiment menu the Device number and device address is displayed. You will have one experiment menu for each Rotamex-5 controller. The device number and device address is used to determine which Rotamex-5 that menu is controlling.
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Page 26: Diagnostic Menu
Rotamex-5 0254-0001 v1.11 Wheel Size. These buttons are used to determine which wheel the Rotamex is configured with. This setting must be set correctly when operating in Linear speed mode(cm/s). Wheel Rotation Direction. This button is used to select which direction the Rod assembly will rotate. -
Page 27: Running Experiment
Rotamex-5 0254-0001 v1.11 Speed Units: These buttons allows the user to switch between RPM and cm/s speed mode. When the instrument is in CM/S speed mode the rod type must also be defined. Rod Type: This button allows the user to define which type of ROD is present. - Page 28 Rotamex-5 0254-0001 v1.11 Rotamex Controller: The Rotamex Controller is used to Enable each channel as the animal is loaded. Once all the animals are loaded press the Enter button to start the experiment.
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Page 29: Experiment Data
Rotamex-5 0254-0001 v1.11 4.6 Experiment Data The Experiment Data is displayed as the animal has fallen from the rota rod. As each animals falls the start time, channel number, subject id, current rod speed and amount of time the animal stayed on the rod without leaving the beams. -
Page 30: Passive Rotation Mode
Rotamex-5 0254-0001 v1.11 4.6.1 Passive Rotation Mode On units that have firmware later then version 2.11 Additional data is provided along with the normal session data. A passive rotation event is defined as the amount of time the animal remained on the rod after it has left the beam for a brief period of time. The absence from the beam could be from the animal's rotation around the rod or a partial fall where the animal only holds unto the rod via his front legs and then crawls back onto the rod. -
Page 31: Data File Format
The Experiment files are saved as a common CSV file format. CSV stands for common separated values. This is easy imported into any type of spread sheet type application. The raw file format is displayed below in notepad form. Columbus Instruments Rotamex-5 1.3 Rotamex-5 number 1 at address 128 Started On:,09/22/05 08:20:41 Experiment Filename:,G:\Rotamex-5\Data\demo experiment.csv [Experiment Setup Info] Start Speed:,0.0...
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