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DUTSCHER FlexA-200 Operation Manual

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Operation Manual
Version 1.0
FlexA-200
Microplate Reader
I

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Summary of Contents for DUTSCHER FlexA-200

  • Page 1 Operation Manual Version 1.0 FlexA-200 Microplate Reader...

  • Page 2: Opening Check

    Foreword Thank you for purchasing our Microplate Reader. This user manual describes how the instrument works and the operation guide, please read carefully before operation and keep for future reference. Opening check Please check the instruments as well as all accessories with packing list when you first open it.

  • Page 3: Safety Warning And Guidelines

    Safety Warning and Guidelines 1. Important information for safe use Users should have a clear main idea on how to use this instrument before operate, do read this user manual carefully. Any improper operation without reading manual is forbidden, otherwise there will be risks in cause accidental injury or electrical shock.
  • Page 4 The operator should not open or repair the Instrument by himself, which will result in losing the qualification of repair guarantee or occur accident. If there is some wrong with the Instrument, please contact manufacturer for repair. Before power on, guarantee the voltage used should be accordant to the voltage needed, and the rated load of electrical outlet should not lower than the demand.

  • Page 5: Table Of Contents

    Contents Foreword..............................II Opening check............................II Safety Warning and Guidelines........................ III 1. Important information for safe use....................III 2. Safety Tips............................. III Chapter 1 Brief Introduction........................1 Chapter 2 Features..........................2 Chapter 3 Instrument Structure......................3 Chapter 4 Installation..........................4 1. Opening check..........................4 2.

  • Page 6: Chapter 1 Brief Introduction

    Chapter 1 Brief Introduction Chapter 1 Brief Introduction This automatic microplate reader FlexA-200 is professional instrument for ELISA , measuring concentration, absorbance, positive or negative of the antibody and antigen in the sample by testing the color of the Enzyme - Linked Immunosorbent Assay (ELISA).

  • Page 7: Chapter 2 Features

    FlexA-200 Microplate Reader Operation Manual Chapter 2 Features Chapter 2 Features Working conditions： Ambient temperature: 10C~40C The relative humidity: 30%~80%(No condensation) Power: AC100-240V 50-60Hz 2A Parameters: Model FlexA-200 Parameter Light source Xenon flash lamp / >10 flashes Wavelength 200～1000nm ≤ 2.5nm...

  • Page 8: Chapter 3 Instrument Structure

    FlexA-200 Microplate Reader Operation Manual Chapter 3 Instrument Structure Chapter 3 Instrument Structure Structure Rear cover Touch screen Roof cover Front door Extension module Plate holder External Button B-type USB Port USB Port LAN Port Air outlet Power Socket Switch ─...

  • Page 9: Chapter 4 Installation

    Chapter 4 Installation Chapter 4 Installation 1. Opening check Each FlexA-200 is thoroughly tested before shipping, but please check again when you receive the instrument and contact your local distributor or manufacturer if: • The outer package is damaged • The outer package has any obvious moisture stains •...
  • Page 10 FlexA-200 Microplate Reader Operation Manual Chapter 4 Installation Note: Attention to the interface of the power adapter, please connect it to the power according to the direction of the above picture. Switch “I/O” button to “I” to turn on the instrument, the front panel will cycle through a start-up and self-test screen.

  • Page 11: Chapter 5 Operation Guide

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Chapter 5 Operation Guide 1. Instrument self-check This chapter introduces default protocol operation, beginning with self-check after power on. Refer to the picture below: Fig 2 User login interface will appear after self-checking, see Fig 3.

  • Page 12: System Settings

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 1 Creation User type Default password Permission Export Method Can not be For all files of Admin, Admin “123456” deleted User and Guest Created by Default is “123456” or All can be...

  • Page 13: Protocol Management

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5 Note: 1. The instrument need to be restarted after date and time settings finished. 2. The function of maintenance is only for manufacturer use, does not open for users.
  • Page 14 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Protocol name can be modified by clicking it directly. The default name of a new protocol is the system time, and it can be modified by manual. A hint will pop-up when a modified protocol not saved.

  • Page 15: Read A Microplate

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide When the protocol list is in unfold state, users can do below operations:  Search: Enter keyword to carry out searching automatically.  Sorting: protocols can be sorted according to “Name”, “Date” and “State”. “demo1”, “demo2”...
  • Page 16 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Meanwhile, the switching bar will turn out to be type select which includes 6 sample types, as well as clear option. Choose the right sample type, the optional bar will change accordingly, then click corresponding wells of the main display area to finish the settings.
  • Page 17 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 9 Note: Elisa plate layout can be carried out before or after testing, for example, users can set all wells as “unknown”, do absorbance test, then layout of plate. The test data and layout are separated, users can modify or analyze the layout of historical data in real time.
  • Page 18 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide functions:  Incubator: this function can make the plate detection chamber up to specified temperature, the temperature displays is the real time temperature.  Wavelength: shows wavelength parameters of the current protocol.
  • Page 19 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 2 Endpoint Kinetic Spectrum ┗ Selected Parameters ┗ Selected Parameters ┗ Selected Parameters ┗ Wavelength ┗ Wavelength ┗ Wavelength ┗Mode ┗Mode ┗Mode ┗Fast ┗Fast ┗Fast ┗Precision ┗Precision ┗Precision ┗ Wavelength ┗...
  • Page 20 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide 4.3 Detect a Elisa plate Click “ ” on screen or “Plate in/out” on the front of the enclosure, place an Elisa plate on the plate holder, attention to the direction please, see Fig 11.

  • Page 21: Result Processing

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide 5. Result processing The interface will stay in “Run” interface after detecting samples in “Run” interface and displays the original absorbance measured under the current protocol. If users want results been analyzed, just switch to “Result”...
  • Page 22  Quadratic polynomial  Cubic polynomial  Quartic polynomial  Point-to-point  Cubic Spline  Logit/Log The absorbance value and concentration value of standard sample can be pretreated by concentration conversion and absorbance conversion, FlexA-200 supports four types as below: ─ ─...
  • Page 23 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide  Linear/Linear (Linear fitting of absorbance value and corresponding concentration)  Linear/Log (Linear fitting of absorbance value and the logarithm of the concentration)  Log/Linear  Log/Log Note: Protocol layout interface must have standard well, different fitting algorithm needs different quantity of standard samples, please check the layout settings when curve fitting failure.
  • Page 24 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 16 Note: protocol layout interface must be set with “Quality” well, otherwise, this button will be not available. 5.2 Kinetics As Fig 17 shows, the main display area is not absorbance values, but kinetic curves.
  • Page 25 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 18 “Calculation” area turns out four types: “Raw Data”, “Blank Subtraction”, “Basic Calculation” and “Kinetic Analysis” as the above Fig 18 shows. For functions and algorithms of “Raw Data”, “Blank Subtraction” and “Basic Calculation”, please see Fig 5.1.
  • Page 26 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 19 5.3 Spectral Analysis See Fig 20, select target wells, click “Graph” button to enlarge the spectral curve, the absorbance of each wavelength shown on the curve. Users can click “Back” button to the graphic main interface.
  • Page 27 FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide  Ration within Spectrum ： set two wavelength values λ1 and λ2, calculate the value of λ1/λ2.  Select Wavelength Range：read measurement values within a set wavelength range.  Select Single Wavelength ：read the measurement value of a single wavelength.

  • Page 28: Report Exporting

    FlexA-200 Microplate Reader Operation Manual Chapter 5 Operation Guide 6. Report exporting Both processed data and raw data can be exported, click “Report” button on the left side to the main interface of reports, see Fig 22. Fig 22 Choose the right format on “File Type” area, four formats are available: ...

  • Page 29: Chapter 6 Maintenance, Storage, Transportation

    FlexA-200 Microplate Reader Operation Manual Chapter 6 Maintenance, storage, transportation Chapter 6 Maintenance, storage, transportation 1. Maintenance  Keep storage environment dry and clean to prevent moisture, corrosion, away from strong electromagnetic interference sources.  Instrument already calibrated before leave factory. User is not allowed to disassembly and make adjustment.

  • Page 30: Chapter 7 Trouble Shooting

    FlexA-200 Microplate Reader Manual Chapter 7 Troubleshooting Operation Chapter 7 Trouble shooting Trouble description Possible reason Solution a. Check the if the instrument The Microplate Reader energized. Power supply failure can not be started b. If the power plug loose c.
  • Page 31 Memo...

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