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USER MANUAL
For Research Use Only
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FastPrep-24™ Classic
The FastPrep-24™ Classic Instrument is a high-speed benchtop homogenizer offering
the ultimate in speed and performance for the lysis of biological samples.
Simultaneous homogenization of up to 24 samples takes place within 40 seconds.
Cat. No.
1 16004500
Revision Date:
#1 16004500-042523
www.mpbio.com
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Related Manuals for MP Biomedicals FastPrep-24 Classic
Summary of Contents for MP Biomedicals FastPrep-24 Classic
- Page 1 USER MANUAL For Research Use Only Circle frame for application images (optional) FastPrep-24™ Classic The FastPrep-24™ Classic Instrument is a high-speed benchtop homogenizer offering the ultimate in speed and performance for the lysis of biological samples. Simultaneous homogenization of up to 24 samples takes place within 40 seconds. Cat.
- Page 2 USER MANUAL: FastPrep-24™ Classic TABLE OF CONTENTS Section Title Here add tabs before and after section titles ... . # Subsection Title Here. Add forced line break + tab before text in second line for cleaner look .....# 1.1.1 Sub-Subsection Title Here. Add forced line break + tab before text in second line for cleaner look .
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Page 3: Precautionary Instructions
1. PRECAUTIONARY INSTRUCTIONS The precautionary instructions found in this section and throughout the manual are indicated by specific symbols. Understand these symbols and their definitions before operating this equipment. The definition of these symbols are as follows: Text with a “CAUTION” indicator will explain possible Safety infractions that could have the potential to cause minor to moderate injury or = CAUTION... - Page 4 Matrix beads on the sample material. Developed for difficult and resistant samples, the FastPrep-24 Classic instrument thoroughly and quickly lyses any tissues and cells. It allows easy and reproducible isolation of stable RNA, active proteins and full- length genomic DNA. Samples and buffers are added to a Lysing Matrix tube containing specialized Lysing Matrix beads.
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Page 5: Installation
Securing Knob (ratchet) 1 pack Compare the packing list to the box contents. If there is a discrepancy, please notify MP Biomedicals. CAUTION: DO NOT LIFT DEVICE BY COVER 3.2 Inspection Inspect the unit for any damage that may have occurred during shipment. -
Page 6: Connecting The Power
USER MANUAL: FastPrep-24™ Classic 3.4 Ventilation Allow 1-2 inches (3-5 cm) of space around the FastPrep-24 instrument for proper ventilation. This unit is FOR INDOOR USE ONLY. Avoid operating in areas of excessive humidity or temperature extremes. 3.5 Connecting the Power The FastPrep-24 can work on 110 VAC/60 Hz or 220 VAC/50 Hz. - Page 7 4.2 Loading and Securing the Samples 1. Loosen the Securing Knob (Ratchet Nut) by rotating counter- clockwise until it comes out (Ref. Fig. 3). 2. Remove the Securing Knob (Ratchet Nut). Securing Knob (Ratchet Nut) Three Step Spoke Plate Fig. 3 3.
- Page 8 USER MANUAL: FastPrep-24™ Classic Slightly lift the Spoke Plate and rotate clockwise so that the retention spokes move away from holes to leave them open for loading (Ref. Fig. 5). Sample Holder Spoke Plate Sample Hole Fig. 5 Load sample tubes into the cavities of the Sample Holder so that they fit snugly in the holes.
- Page 9 Uplift and rotate the Spoke Plate counter-clockwise so that the retention spokes take the position above each sample tube cap. Place the Securing Knob (Ratchet Nut) on the assembly to tighten it completely (Ref. Fig. 7). The Securing Knob should be ratcheted several times (not just once). It is good practice to push down on the nut while twisting to secure the part, make sure you hear several clicking sounds.
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Page 10: Preparing The Fastprep-24 For Operation
USER MANUAL: FastPrep-24™ Classic 4.3 Preparing the FastPrep-24 for Operation FastPrep is delivered with an Emergency Switch. Remove the Emergency Switch by pulling and then turn the main Rocker Switch ON (backside of the machine). It is not necessary to pull out the Emergency Switch each time the instrument is used - simply use the Rocker Switch. - Page 11 To change the default Tube Holder setting, press SET key. LCD will show 4.0 m/s Use key until MP:24x2 is displayed, then press SET key. LCD will start flashing to indicate the sample tube holder can be changed using or key.
- Page 12 USER MANUAL: FastPrep-24™ Classic NOTE: When Speed, Tube Holder or Run Time is flashing (in selectable mode), the user can still start the unit directly by pressing RUN key twice. These values will be temporary and they will not be saved unless SET key is pressed and LCD stops flashing.
- Page 13 Once Speed has been set, press SET key to save in program 1. Now the LCD will show P1 TH MP . Letter “MP” will be flashing. Use or key to set the desired Tube Holder. Once Tube Holder has been set, press SET key to save in Program 1. Now the LCD will show P1 T 20 .
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Page 14: Specifications
USER MANUAL: FastPrep-24™ Classic 5. SPECIFICATIONS Programmable run time, tube holder and speed; Controls LCD readout Time Range: 1-60 sec Speed Range: 4.0-6.5 m/s Acceleration <2 seconds to maximum speed Deceleration < 2 seconds to stop Weight 17.9 kg Power Requirement 110 VAC/60 Hz or 220 VAC/50 Hz, 500 W Operating Temperature 5°C to 35°C... -
Page 15: Warranty And Liability
WARRANTY & LIABILITY WARRANTY The product warranty extends to the original consumer/purchaser of the product. WARRANTY DURATION This product is warranted to the original consumer for a period of one (1) year from the original purchase date. Extended warranty duration is available upon request. - Page 16 During the above one-year warranty period, a product with a defect will be repaired or replaced with a reconditioned comparable unit at MP Biomedicals option when the product is returned to MP Biomedicals nearest facility or distributor. The repaired or replacement product will be in warranty for the balance of the one-year warranty period and an additional one-month period.
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Page 17: Appendix 1: Maintenance & Cleaning
APPENDICES 7.1 APPENDIX 1: Maintenance & Cleaning Maintenance: The FastPrep-24 instrument requires no scheduled maintenance. Clean surfaces immediately after contact with sample solutions or reagents. Fig. 8 (Spring Replacement) Spring Replacement Instructions: For the long running life of the instrument, 8 extra springs are provided. If a spring breaks, follow the steps below (Ref. - Page 18 USER MANUAL: FastPrep-24™ Classic Fuse Replacement: Rocker Switch AC Receptacle Fuse Receptacle Fig. 9 (Fuse Replacement) CAUTION: Disconnect input power before replacing fuse. For continued fire protection, replace only with specified type and rated fuse. Fuse rating is 10 AMP. 2 extra fuses are provided with accessories. If a fuse blows, follow the steps below for replacement (Ref.
- Page 19 Sample Tubes Bowl Fig. 10 (Cleaning) Cleaning: The FastPrep-24 instrument should be cleaned if reagents or sample solutions spill on or inside the unit. If a sample tube leaks during a run, the solution will be sprayed on the bowl. Always clean any spray or spills immediately using a damp paper towel.
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Page 20: Appendix 2: An Explanation Of Fastprep-24 Instrument Speed Settings
The FastPrep-24 instrument has been specifically designed to allow operation within an ideal range of parameters for disrupting membranes from a wide variety of cell types. When used with cell-specific protocols and kits (MP Biomedicals), cell membrane disruption and nucleic acid yield is maximized. -
Page 21: Appendix 3: Fastprep-24 Product Line
7.3 APPENDIX 3: FastPrep-24 Product Line The FastPrep-24 instrument is part of a complete purification system for the researcher. This system is composed of three families of products: The FastPrep-24 instrument: for lysis of cells prior to DNA, RNA and protein purification. - Page 22 USER MANUAL: FastPrep-24™ Classic Lysing Matrices are critical components of the FastPrep-24 sample preparation system. MP Biomedicals receives matrix particles from proven vendors and ensures the unprocessed material meets high qualitative standards. Matrix particles are then prepared and dispensed under a rigorous set of proprietary conditions that offer customers a premium product that can be immediately used with confidence.
- Page 23 Lysing Matrix G Lysing Matrix J Each impact-resistant 2.0 mL Each impact-resistant 2.0 mL tube contains silicon carbide and tube contains 2 mm yellow 2 mm glass beads. Lysing Matrix zirconia beads and white G tubes have brown caps. granules. Lysing Matrix J tubes have pink caps.
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Page 24: Appendix 4: Accessories & Spare Parts
7.4 APPENDIX 4: Accessories & Spare Parts Description Cat. No. Driver PCB 116004503C Display PCB 116004503B SMPS 48V 116003502 Motor BLDC W/ 116004504K Shaft Bowl 116002505 WARNING: This part must be replaced by a qualified technician or MP Biomedicals technical service... - Page 25 Description Cat. No. Polycarboate-Dome 116002506 with cap and stoper Dome Rubber 116002507 Gasket Dome Clip 116002508 Lock Pins for 116002509 dome locking Ratchet Nut 116004510...
- Page 26 USER MANUAL: FastPrep-24™ Classic Description Cat. No. Ratchet Bolt 116002511 Sample Holder Set 116002512 3 STEP Assem- bly with studded 116002513 bearings Spring assembly set 116002514 with hooks Rubber Shoe 116002515...
- Page 27 Description Cat. No. Fuse 10 Amp-UL 116002516 European AC Cord 116002518 3Pin flat AC Cord 116002519 USA (UL) AC Receptacle with 116002520 fuse Rocker Switch-UL 116002521...
- Page 28 USER MANUAL: FastPrep-24™ Classic Description Cat. No. Emergency Switch 116003524 Micro Switch 116002534 Gasket 116004535 Inner Ring 116004541 Outer Ring 116004542 WARNING: This part must be replaced by a qualified technician or MP Biomedicals technical service...
- Page 29 Notes:...
- Page 30 MP BIOMEDICALS www.mpbio.com AMERICAS: 800.854.0530 custserv.na@mpbio.com EUROPE: 00800.7777.9999 custserv.eur@mpbio.com APAC: +65 6775.0008 custserv.ap@mpbio.com...
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