MP Biomedicals FastPrep-24 Instruction Manual
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MP Biomedicals FastPrep-24 Instruction Manual

Tissue and cell homogenizer
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Instruction Manual
Instruction Manual
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www.mpbio.com
MP Biomedicals • 29525 Fountain Parkway • Solon, OH 44139 • tel: 1.800.854.0530 • fax: 1.800.334.6999

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Summary of Contents for MP Biomedicals FastPrep-24

  • Page 1 Instruction Manual Instruction Manual One Call One Source A World of Biotechnology Reagents www.mpbio.com MP Biomedicals • 29525 Fountain Parkway • Solon, OH 44139 • tel: 1.800.854.0530 • fax: 1.800.334.6999...
  • Page 2 Precautionary Instructions The precautionary instructions found in this section and throughout this manual are indicated by specific symbols. Understand these symbols and their definitions before operating this equipment. The definition of these symbols are as follows; Text with a "CAUTION" indicator will explain =CAUTION possible Safety infractions that could have the potential to cause minor to moderate injury or...
  • Page 3 Model #6004-500 www.mpbio.com...

  • Page 4: Table Of Contents

    Table of Contents Section I Introduction ..................5 Section II Installation ...................6 2.1 Unpacking....................6 2.2 Inspection....................6 2.3 Set-up..................... 7 2.4 Connecting the Power................7 Section III Operation....................8 3.0 Controls & Functions ................8 3.1 Loading & Securing the Samples.............. 9 ®...

  • Page 5: Section I Introduction

    I. INTRODUCTION ® The FastPrep -24 Instrument is a high-speed benchtop homogenizer offering the ultimate in speed and performance for the lysis of biological samples. Indeed, simultaneous homogenization of 24 samples in 2ml tubes,12 samples in 15ml tubes ,48 samples in 2ml tubes or 2 samples in 50ml tubes takes place within 40 seconds.

  • Page 6: Section Ii Installation

    2.2 Inspection Inspect the unit for any damage that may have occurred during shipment. Should there be any damage, report it to the carrier and contact MP Biomedicals immediately. Save the packaging material in the event a return is necessary.

  • Page 7: Connecting The Power

    2.4 Connecting the Power ® The FastPrep 24 can work on 110 VAC/60 Hz or 220 VAC/50 Hz. Make sure the rocker switch located on the rear panel is OFF when connecting the power. Connect the power cord to the Instrument (power fixture is at the back side) and plug it in to a compatible outlet.

  • Page 8: Loading & Securing The Samples

    3.1 LOADING & SECURING THE SAMPLES 1. Loosen the securing knob (Ratchet Nut) by rotating counter-clockwise until it come out (Ref. Fig. 3) 2. Remove the securing knob (ratchet Nut). Securing Knob (Ratchet Nut) Three Step Spoke Plate Fig. 3 3.
  • Page 9 4. Sightly uplift the spoke plate and rotate clockwise so that retention spokes move away from holes to leave them open for loading (Ref. Fig. 5). Sample Holder Retention Spoke Plate Fig. 5 5. Load sample tubes into the cavities of the sample holder, so that they fit snugly in the holes.
  • Page 10 7. Now uplift and rotate the spoke plate counter-clockwise so that the retention spokes take the position above each sample tube cap. Place the securing knob (ratchet nut) on this assembly to tighten it completely (Ref. Fig. 7). Retention Spoke Plate Sample Tube Fig.

  • Page 11: Preparing The Fastprep 24 For Operation

    ® PREPARING THE FastPrep 24 FOR OPERATION 1. Turn the main Rocker Switch ON ( Make sure Emergency Switch is pulled out). ® 2. Factory-set default values automatically program FastPrep -24 to operate at speed 4.0 m/sec, Tube holder MP:24*2 and run time 20 seconds (to override these values.
  • Page 12 In case Default sample tube holder is to be changed, then press SET key, LCD will show 4.0 M/S , now use key to reach upto the message MP:24*2. Press SET key when this is displayed, LCD will start Flashing, to indicate the sample tube holder can be changed using key, Once tube holder is selected, press SET key again ,Flashing of LCD will stop to indicate that tube holder has been selected.

  • Page 13: Save Programs

    Press RUN Key to stop the instrument at any time during a run LCD will show Stop the program will go back to main menu with last selection . Turn OFF the instrument by pressing rocker switch. SAVE PROGRAMED COMBINATIONS During Program 1 to 5, Tube holder selection will be displayed as PX TH MP - MP:24*2 - QuickPrep Adapter,( Supplied with instrument)
  • Page 14 EMERGENCY NOTE: In case any difficulties Push the Emergency Switch to stop the instrument at any time (on the front panel of the instrument) Ref. Fig. 1 In this, the instrument will be completely isolated from AC mains. Pull the emergency switch again to make the instrument ready to Restart.

  • Page 15: Section Iv Specifications

    IV SPECIFICATIONS Controls: Programmable run time, tube holder and speed; LCD readout Time: Range: 1-60 Sec Programmable in 1 sec increments Speed : Range: 4.0-6.5m/sec Programmable in 0.5 m/sec increments Acceleration: <2 seconds to maximum speed Deceleration: <2 seconds to stop Weight: 17.9 Kg Power...

  • Page 16: Section V Warranty And Liability

    V WARRANTY & LIABILITY WARRANTY This product warranty extends to the original consumer/ purchaser of the product. WARRANTY DURATION This product is warranted to the original consumer for a period of one (1) year from the original purchase date. Extended warranty duration on request. WARRANTY COVERAGE This product is warranted against defective materials.
  • Page 17 LEGAL REMEDIES This warranty gives you specific legal rights, and you may also have other rights that vary from state to state. WARRANTY PERFORMANCE During the above one-year warranty period, a product with a defect will be repaired or replaced with a reconditioned comparable unit at distributors option when the product is returned to the distributor.

  • Page 18: Section Vi Appendices

    VI APPENDICES APPENDIX I MAINTENANCE & CLEANING ® Maintenance: The FastPrep -24 Instrument requires no scheduled maintenance. Clean surfaces immediately after contact with sample solutions or reagents. Fig. 8 (Spring Replacement) Spring Replacement Instructions: For the long running life of the instrument 8 extra springs are provided.
  • Page 19 Fuse Replacement: Rocker Switch AC Receptacle Fuse Receptacle Fig. 9 (Fuse Replacement) CAUTION: Disconnect input power before replacing fuse. For continued fire protection replace only with specified type & rated fuse. Fuse rating is 10 Amp. 2 extra fuses are provided with accessories. If a fuse blows, follow the steps below for replacement (Ref.
  • Page 20 Bowl Sample Tubes Fig. 10 (Cleaning) ® Cleaning: The FastPrep -24 Instrument should be cleaned if reagents or sample solutions spill on or inside the unit. If a sample tube leaks during a run, the solution will be sprayed on the bowl. Always clean up any spray or spills immediately using a damp paper towel.

  • Page 21: Appendix 2 An Explanation Of Fastprep -24 Instrument Speed Settings

    APPENDIX 2 ® AN EXPLANATION OF FastPrep -24 INSTRUMENT SPEED SETTINGS ® The cell disruption process during a FastPrep -24 Instrument run is caused by ® the collision of matrix and sample within the FastPrep -24 Instrument sample tube. The rate of collision and energy of impact. (both of which determine the ®...

  • Page 22: Appendix 3 Fastprep -24 Product Line

    APPENDIX 3 ® FastPrep -24 PRODUCT LINE ® The FastPrep -24 Instrument is part of complete PURIFICATION SYSTEM for the researcher. This system is composed of three families of products: ® FastPrep -24 Instrument for lysis of cells prior to DNA, RNA and protein purification Lysing Matrix Tubes that contain small particles (the lysing matrix) ®...
  • Page 23 ® Lysing Matrices are critical components of the FastPrep 24 sample Preparation system. MP Biomedicals receives matrix particles from proven vendor and ensures the unprocessed material meets high qualitative standards. Matrix particles are then prepared and dispensed under a rigorous set of proprietary conditions that offer customers a premium product that can be immediately used with confidence.
  • Page 24 Lysing Matrix E Each impact-resistant 2.0 ml tube contains 1.4 mm ceramic spheres, 0.1 mm silica spheres, and one 4 mm glass bead. Lysing Matrix E tubes have ® purple caps and are found in the FastDNA SPIN Kit for Soil for isolation of any type of DNA found in soil or other environmental samples.
  • Page 25 Lysing Matrix J Each impact-resistant 2.0 ml tube contains 2 mm yellow zirconia beads and white granules. Lysing Matrix J tubes have pink caps. Lysing Matrix K Each impact-resistant 2.0 ml tube contains 0.8 mm zircon beads. Lysing Matrix K tubes have brown caps and are found in the GENECLEAN for ®...
  • Page 26 ® FastPrep Kits: Ready-to-Use Protocols ® The Fastprep -24 System includes a selection of kits for nearly every application. These kits are summarized in the table below. Sample Matrix Most important factors 1. Unsheared DNA ® FastDNA Kit 2. High quantity ®...
  • Page 27 ® ® FastDNA and FastDNA SPIN Kits ® ® The FastDNA and FastDNA SPIN Kits quickly and efficiently isolate genomic ® DNA from a wide variety of sources., Designed for use with the FastPrep instruments, plants, animal tissues, yeast, bacteria, algae, fungi and many other samples are easily lysed within 40 seconds.
  • Page 28 ® FastDNA SPIN Kit for Soil ® The FastDNA -24 SPIN Kit for Soil quickly and efficiently isolates PCR-ready genomic DNA directly from soil samples in less than 30 minutes. Designed for ® use with the Fastprep instrument, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds.
  • Page 29 ® FastRNA Pro Isolation Kits ® The FastPrep -24 System is also ideal for use in RNA isolation. Several kits are available for researchers requiring RNA isolation. These are : ® • FastRNA Pro Blue Kit (Bacteria) ® • FastRNA Pro Red Kit (Yeast) ®...

  • Page 30: Appendix 4 Accessories And Spare Parts For Fastprep -24

    Accessories and Spare Parts for FastPrep -24 Package Description Cat. No. Size 6004503A Driver PCB 6004503B Display PCB 6003502 SMPS 48V 6003504 A Motor 6002505 Bowl Warning: This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service www.mpbio.com...
  • Page 31 Polycarbonate-Dome with cap and Stopper pack of 2 6002507 Dome Rubber Gasket 6002508 Dome Clip 6002509 Lock pins for Dome Locking pack of 2 6002510 Rachet nut Warning: This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service www.mpbio.com...
  • Page 32 6002513 3 STEP-Assembly with studded bearings 6002514 Spring assembly set with hooks 6002515 pack of 4 Rubber Shoe pack of 4 6002516 Fuse 10 Amp-UL Warning: This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service www.mpbio.com...
  • Page 33 Cat. No. Size 6002518 European AC Cord 6002519 3Pin flat Ac Cord-for USA (UL) 6002520 AC Receptacle with fuse 6002521 Rocker Switch-UL 6003524 Emergency Switch Warning: This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service www.mpbio.com...
  • Page 34 IMPORTANT : As the design and manufacture of our product is subject to constant improvement, the product supplied may differ in some details from the specification and...
  • Page 35 Instruction Manual Instruction Manual Ver. 4.0 Worldwide Ordering and Technical Support www.mpbio.com MP Biomedicals • 29525 Fountain Parkway • Solon, OH 44139 • tel: 1.800.854.0530 • fax: 1.800.334.6999...

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