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July. 2010 INSTRUCTION MANUAL Vacuum Blotter Cat # INSB8100 Cat. # INSB8101 MP Biomedicals Europe MP Biomedicals North America Tel : 00800 7777 9999 Tel : 800 854 05 30 Fax : 00800 6666 8888 Fax : 800 934 69 99 custserv.eur@mpbio.com...
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Vacuum Blotter Description The Vacuum Blotter consists of (see illustration on the back cover): • Compact buffer collection reservoir with one seal and four catches for adjusting the sealing frame • Porous plastic divider • Built-in regulated vacuum pump • Command panel with (from left to right) manometer, vacuum control knob, spirit level and On/Off power switch •...
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Installation Set the instrument on a horizontal work bench with the brainsick in front of you. Make sure the Vacuum Blotter is horizontal using the spirit level and the adjustable feet. Connect the apparatus to the power supply (220V, 50 Hz or 110V, 60 Hz depending on the model delivered).
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• A Whatman (Type 3MM) paper sheet to protect the membrane • The transfer membrane (such as MP Biomedicals Positive, Neutral or HCD) • The sealing mask taking care that it is correctly positioned on the seal (no folds) • The gel containing the material to be transferred Note: If the gel splits in the course of the experiment, put the gel pieces together and glue them together by pouring some molten agarose on the splits.
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9) Cleaning. After use, discard the Whatmann paper sheet, remove the sealing mask and the porous plate and rinse them in running water. They can be re-used. Draining and maintenance Pull the apparatus forward until its front foot is no longer resting on the bench. In this position, the reservoir is tipped forward.
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0 to -100 mbar Accuracy of the vacuometer: Class 1.5 ORDERING INFORMATION Cat. # Description INSB8100 Vacuum Blotter 220V - 50 Hz Delivered with 2 plastic masks INSB8101 Vacuum Blotter 110V - 60 Hz Delivered with 2 plastic masks INSB8302...
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