Hygiena foodproof 2300 92 Manual

Listeria monocytogenes detection lyokit

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foodproof
®
Listeria
monocytogenes
Detection LyoKit
MANUAL
Documentation for the qualitative detection of
Listeria monocytogenes
Order No. KIT 2300 92 / KIT 2300 93 / KIT 2300 94

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Summary of Contents for Hygiena foodproof 2300 92

  • Page 1 foodproof ® Listeria monocytogenes Detection LyoKit MANUAL Documentation for the qualitative detection of Listeria monocytogenes Order No. KIT 2300 92 / KIT 2300 93 / KIT 2300 94...
  • Page 2 foodproof ® Listeria monocytogenes Detection LyoKit Order No. LP: KIT 2300 92 RP: KIT 2300 93 DP: KIT 2300 94 Kit for 96 reactions (lyophilized) for a maximum of 94 samples Store kit at 2 °C to 8 °C For testing of food and environmental samples Approvals: LICENSE NUMBER...
  • Page 3: Table Of Contents

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit TABLE OF CONTENTS 1. OVERVIEW ..................4 1.1 General Information ................4 1.2 Applicability ....................4 1.3 Kit Contents ..................... 5 2. INSTRUCTIONS ................6 2.1 Required Material ..................6 2.2 Precautions and Preparations ..............
  • Page 4: Overview

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit OVERVIEW 1. OVERVIEW 1.1 General Information Number of Reactions The kit is designed for 96 reactions with a final reaction volume of 25 µl each. Up to 94 samples plus positive and negative control can be analyzed per run.
  • Page 5: Kit Contents

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit OVERVIEW 1.3 Kit Contents A schematic representation of the foodproof Listeria monocytogenes Detection LyoKit ® with all its components. LP: KIT 2300 92 RP: KIT 2300 93 DP: KIT 2300 94 Component Details 12 x 8-tube strips, prefilled with lyophilized ready-to-use PCR mix.
  • Page 6: Instructions

    INSTRUCTIONS 2. INSTRUCTIONS 2.1 Required Material Most of the required equipment and reagents are available through Hygiena Please contact us for further information. Use a real-time PCR cycler suitable for detection of respective probes as well as for using low or regular profile strip tubes.
  • Page 7: Precautions And Preparations

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit INSTRUCTIONS 2.2 Precautions and Preparations The kit provides all reagents required for the PCR. However, in order to achieve reliable results, the entire assay procedure must be performed under nuclease-free conditions. Follow the instructions below to avoid nuclease, carry-over, or cross-contamination: …...
  • Page 8: Enrichment And Dna Extraction

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit INSTRUCTIONS 2.3 Enrichment and DNA extraction The foodproof Listeria monocytogenes Detection LyoKit is intended for the rapid ® detection of Listeria monocytogenes isolated by foodproof DNA extraction methods ®...
  • Page 9: Procedure

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit INSTRUCTIONS 2.4 Procedure PREPARATION OF THE PCR MIX This protocol describes how to perform the analysis of DNA extracts by real-time PCR. Take appropriate precautions to prevent contamination, e.g. by using fi lter tips and wearing gloves. 2.4.1 Workflow 1.
  • Page 10: Program Setup

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit INSTRUCTIONS 2.4.2 Program Setup Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels: u FAM (L. monocytogenes), and VIC (Internal Control). As an alternative to VIC, HEX can be used. For the PikoReal 24, Yakima Yellow has to be ®...
  • Page 11: Troubleshooting

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit INSTRUCTIONS 2.5 Troubleshooting Problem Possible Cause Recommendation Squashed or Wrong tube format. Choose the correct tube format for crooked tubes, your cycler. Tube profile and instrument or open / compatibility chart is available online: www.
  • Page 12: Support

    DNA/RNA extraction. positive. Repeat the complete experiment with fresh batches of all reagents. Always handle samples, kit components www.hygiena.com/technical-support-request and consumables in accordance with commonly accepted practices to prevent carry-over contamination. Add positive controls after sample and negative control reaction vessels have been Our aim is to provide you with a solution as quickly and effectively as possible.
  • Page 13: Additional Information

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit ADDITIONAL INFORMATION 3. ADDITIONAL INFORMATION 3.1 Testing Principle The foodproof kit provides all necessary reagents and a control template for reliable ® interpretations of results. To ensure maximum reliability of the kit and to prevent misinterpretation of negative results due to inhibition of the amplifi cation, an Internal Control (IC) is included.
  • Page 14: Trademarks

    ® ® of BIOTECON Diagnostics GmbH. Hygiena is a registered trademark of Hygiena. Other brand or product names are trademarks of their respective holders. 3.3 Reference Number The reference number and original BIOTECON Diagnostics article numbers: R 602 23 -1, R 602 23 -2, and R 602 23 -3.
  • Page 15: Annex 1

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit ANNEX ANNEX 1 AOAC-RI #070401 Extension Table for the foodproof ® Listeria monocytogenes Detection LyoKit The following table shows the recommended enrichment time for different food matrices with enrichment in Half Fraser broth in combination with different foodproof DNA extraction ®...
  • Page 16: Annex 2

    KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit ANNEX ANNEX 2 NordVal #025 Extension Table for the foodproof ® Listeria monocytogenes Detection LyoKit The following table shows the recommended enrichment time for the tested food categories and environmental samples in Actero Listeria Enrichment Media (ALEM) and Half Fraser broth in combination with different foodproof DNA extraction procedures that have...
  • Page 17 KIT 2300 92 / 93 / 94 - Listeria monocytogenes Detection LyoKit Sample size: 25 g + 150 ml Actero Listeria Enrichment Media or 225 ml Half Fraser broth 1 swab + 10 ml Actero Listeria Enrichment Media or 10 ml Half Fraser broth 1 sponge + 100 ml Actero Listeria Enrichment Media or 100 ml Half Fraser broth 1 wipe + 225 ml Actero Listeria Enrichment Media or 225 ml Half Fraser broth For sampling after cleaning process, premoisten:...
  • Page 18 Hygiena LLC CA 93012 Camarillo www.hygiena.com/technical-support-request Manufactured by BIOTECON Diagnostics GmbH Hermannswerder 17 14473 Potsdam Germany bcd@bc-diagnostics.com www.bc-diagnostics.com...

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Foodproof 2300 93Foodproof 2300 94

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