Agilent Technologies G1978A Setup Manual
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Agilent Technologies G1978A Setup Manual

Agilent Technologies G1978A Setup Manual

Multimode for 6210/6220 tof
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Agilent G1978A
Multimode for
6210/6220 TOF
Set-Up Guide
Agilent Technologies

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  • Page 1 Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide Agilent Technologies...
  • Page 2 Notices Warranty Safety Notices © Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in The material contained in this docu- any form or by any means (including elec- ment is provided “as is,” and is sub-...
  • Page 3 Multimode for 6210/6220 TOF. Installation This chapter contains information to prepare the TOF instrument for the G1978A multimode source and to replace the existing source with the multimode source. TOF Software and TOF Firmware Upgrade for Multimode This chapter contains information to update your software and firmware for the multimode source.
  • Page 4 Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 5: Table Of Contents

    Contents Installation Installation Step 1. Prepare to install Step 2. Remove the TOF E-Tub Electronics Step 3. Change the firmware chips on the Analyzer and Power Distribution Boards Ω Step 4. Install the three 10M cables Step 5. Install the HV control PCA and cables Step 6.
  • Page 6 Contents Step 9. Run each of the methods created Step 10. Calculate the response of Multimode Demo Step 11. Fill out Multimode Report for calculation of peak heights Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 7: Installation

    To convert between the ESI, APCI or APPI and the multimode ion source This chapter contains instructions to install the G1978A Multimode Source. You need to make changes to the instrument as part of the installation. The multimode source is supported with the following software: •...
  • Page 8 Installation APCI Corona HV cable +/- 2kv APCI High voltage Vaporizer Heater Cable Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 9: Installation

    Installation Installation Step 1. Prepare to install 1 Check that you have these parts: • Bundled LC/MSD Multimode ESI/APCI Source (p/n G1978A) • LC/MSD Multimode ESI/APCI Source (p/n G1978-65239) • LCMSD-TOF MM ESI/APCI Enablement Kit (p/n G1978-60251) • TOF Software A.02.02 or higher •...

  • Page 10: Step 2. Remove The Tof E-Tub Electronics

    Installation 8 In the MS TOF tab, click Parameters and select Negative ion polarity. Click Save As to save the negative tune parameters as d:\Program Files\Agilent\ TOF Software\Tune\NegativeESI.prm. 9 Right-click on the TOF icon in the Status pane and click Vent. 10 After the TOF system has completed the vent process, turn off the front switch and the main breaker.

  • Page 11: Step 3. Change The Firmware Chips On The Analyzer And Power Distribution Boards

    Installation Step 3. Change the firmware chips on the Analyzer and Power Distribution Boards The Main PLCC Firmware chip U129 must be changed on the Analyzer 3 PCA for source identification (p/n G1978-80067). Two chips on the power distribution board U6 (p/n G1978-80100) Programmed ROM MM LON and U18 Chip EPROM (p/n G1978-80200) will also be changed.
  • Page 12 Installation Figure 2 Carefully remove the chip from the socket 5 In the Firmware Upgrade Kit, find the chip with the part number G1978-80067. 6 Find the correct orientation of the chip so that it fits into the socket. Press the chip into the socket.
  • Page 13 Installation U6 Socket U18 Socket Figure 3 Remove the U6 and U18 chips from their sockets 9 Locate the chip with the part number G1978-80200. Find the correct orientation of the chip so that it fits into the socket. Press the G1978-80200 chip into the U18 socket.

  • Page 14: Ω Cables

    Installation Step 4. Install the three 10MΩ cables 1 While the instrument is vented and the analyzer board is out, install the cables in series with the output cable for the Vcap/Vchamber and APCI power supplies. The cables can be installed with the analyzer board installed.
  • Page 15 Installation 3 Connect the other M-F 10MΩ ballast MIDGI cable (p/n G1978-60806) in series with the VChamber power supply cable and plug it back into to VChamber power supply output. 4 Connect the M-F 10MΩ ballast SCID cable (p/n G1978-60805) in series with the Vcap cable then plug it back into to VCap output of the power supply.

  • Page 16: Step 5. Install The Hv Control Pca And Cables

    Installation Step 5. Install the HV control PCA and cables 1 Attach the attach the RS-232 cable to the HV and control PCA power supply RS 232 connector. Figure 7 Attaching RS-232 Cable 2 Remove the cover from the HV module (p/n G1978-60050) Figure 8 Removing HV Module Cover 3 Attach the HV and control PCA to the tube.
  • Page 17 Installation Plastic cable clamp Figure 9 Plastic cable clamp Plastic cable clamp removed Figure 10 Cable clamp removed and cable moved down 5 Attach the HV and control PCA power supply to the tube with the self-trapping screw supplied. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 18 Installation Attach the HV and control PCA power supply to the Figure 11 Self tapping screw 6 Clamp the top cover of the HV and control PCA power supply with screws provided to the support bracket. Attach the HV and control PCA power supply to the tub HV and control PCA...
  • Page 19 Installation Serial connector on Smart card Figure 13 RS-232 cable connections 7 Connect the (RS-232) from HV module to the Serial connector on Smart card 4 interface. See Figure 8 Connect the 15V dc power supply to the HV and control PCA. See Figure 14 Figure 9 Connect the other end of the 15V dc power supply into an 110V ac outlet...
  • Page 20 Installation 15V dc connection to HV Module Figure 14 RS 232 cable connections 15V dc connection to HV module 110 VAC Power cord 15V dc power supply Figure 15 Power cord and 15V dc supply Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 21: Step 6. Attach The Multimode Source To The Instrument

    Step 6. Attach the multimode source to the instrument 1 Remove the currently installed spray chamber (if applicable) from the spray chamber mount. Remove the shipping cover from the multimode source spray chamber. Figure 16 Multimode source 2 Install the multimode source on the spray chamber mount. See Figure Spray chamber mount Spray chamber mount...
  • Page 22 Installation 3 Screw the End Cap Assembly into the holder for the end cap. See Figure Note that the field shaping electrodes are in the 9 o'clock and 6 o'clock positions. 4 Loosen the end plate screws on each side to adjust the field shaping electrodes position.
  • Page 23 Installation APCI Heater 15V dc connection ESI Charging Electrode power APCI High Voltage Figure 19 Cable connections Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 24: Step 7. Install The Nebulizer, Corona Needle, And Gas Connections

    Installation Step 7. Install the nebulizer, corona needle, and gas connections 1 Install the nebulizer on the multimode source spray chamber. Never use a nebulizer spacer on a multimode source. 2 Connect the 1/8-inch nebulizing gas tubing from the LC/MS instrument mainframe to the nebulizer gas fitting 3 Connect the LC/MS sample tubing to the nebulizer zero dead volume fitting.
  • Page 25 Installation 6 Switch Calibrant Bottle B to the Multimode Calibrant (p/n G1969-85020) for TOF. Figure 21 Calibrant For TOF (p/n G1969-85020) 7 Re-install all covers. Figure 22 All covers re-installed Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 26: Changing Sources

    Installation Changing Sources To convert between the ESI, APCI or APPI and the multimode ion source Do this step if you want to change the source between ESI, APCI or APPI and the multimode source. To change to the multimode source for the first time, go “Installation”...
  • Page 27 Installation Standard spray shield Capillary cap Figure 23 Standard spray shield 6 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap See Figure Capillary cap Figure 24 Capillary Cap 7 If needed, moisten a clean cloth with isopropyl alcohol and gently wipe the front of the capillary then reinstall capillary cap.
  • Page 28 Installation Capillary Figure 25 Capillary 8 After cleaning the capillary and cap, place the capillary cap back on capillary. Install the new spray shield with field shaping electrodes. Figure 26 End Cap Assembly, Multimode (p/n G1978-60060) 9 Screw the multimode spray shield into the holder for the spray shield. See Figure Note that the field shaping electrodes are in the 9 o'clock and 6 o'clock positions.
  • Page 29 11 Remove any cables such as APCI vaporizer heater cable and APCI high voltage cable and any other cables such as RS 232 cable on APPI source. When converting back to any of the sources other than the G1978A the HV module, C A U T I O N disconnect the +15V DC power from the module.
  • Page 30 Installation Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...

  • Page 31: Tof Software And Tof Firmware Upgrade For Multimode

    Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide TOF Software and TOF Firmware Upgrade for Multimode Step 1. Upgrade the TOF Software Step 2. Upgrade the TOF firmware Step 3. Pump down the LC/MSD TOF and condition the high voltage...

  • Page 32: Step 1. Upgrade The Tof Software

    TOF Software and TOF Firmware Upgrade for Multimode Step 1. Upgrade the TOF Software 1 Back up the following folders in the C:\Program Files\Agilent\TOF Software folder to a safe location. • DA methods • methods • worklists Methods and mass lists will be overwritten when the new software is installed.
  • Page 33 BootP. You can also configure the LC with a fixed IP addresses. Please refer to the CD labeled “Agilent Technologies LAN Card G1369A” (p/n G1369-90000) for more information. 19 Click Next after specifying the location for data like methods and reports to be stored.
  • Page 34 TOF Software and TOF Firmware Upgrade for Multimode 25 After reloading software, configure the logical instrument. The TOF and optionally the LC and ADC are configured into one logical instrument. This must be done before using the TOF Software system. a Click Start >...

  • Page 35: Step 2. Upgrade The Tof Firmware

    TOF Software and TOF Firmware Upgrade for Multimode Step 2. Upgrade the TOF firmware 1 Install the TOF Firmware Update tool. Use File Explorer and open the CD-ROM drive for the Installation CD. 2 Open the folder Support\TOF Firmware. 3 Double-click MSFirmwareUpdate_1.16.exe. The WinZip self-extractor dialog appears.

  • Page 36: Step 3. Pump Down The Lc/Msd Tof And Condition The High Voltage Electronics

    TOF Software and TOF Firmware Upgrade for Multimode Step 3. Pump down the LC/MSD TOF and condition the high voltage electronics Before you try tuning the LC/MSD TOF, the high voltage electronics need to be conditioned. 1 Click Start > Programs > Agilent > TOF Software > Tools > TOF Diagnostics.

  • Page 37: Installation Verification

    Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide Installation Verification Step 1. Auto tune Step 2. Set up method names and parameters Step 3. Create MMCHECKTOF_EI_POS.m Step 4. Create MMCHECKTOF_EI_NEG.m Step 5. Create MMCHECKTOF_CI_POS.m Step 6. Create MMCHECKTOF_CI_NEG.m Step 7. Create MMCHECKTOF_MX_EI POS_CI POS.m Step 8.

  • Page 38: Step 1. Auto Tune

    Installation Verification Step 1. Auto tune MassHunter TOF Acquisition for TOF A.02.02 Before the TOF system with multimode source can be used, the installation and verification tasks must be completed. Auto tune is done in mixed mode, with the TOF G1969-85000 ESI-L Low Concnetration Tuning Mix. 1 Click File >...
  • Page 39 Installation Verification Figure 28 Positive Autotune report for multimode source Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 40 Installation Verification 4 Switch the polarity to negative and run autotune. The instrument will generate a negative Autotune report for the multimode source. Figure 29 Negative Autotune report for multimode source Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 41 Installation Verification 5 Load the associated polarity switching method positive. Example: MMIautotunePSPos.m 6 Click the Tune tab and select Autotune. The instrument will generate a polarity switching positive report only for MMIautotunePSPos.m. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 42 Installation Verification 7 Load the associated polarity switching method negative. Example: MMIautotunePSNeg.m. The instrument will generate a polarity switching negative report only for MMIautotunePSNeg.m. The instrument is ready for use with fast polarity switching tunes. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 43 Installation Verification For MassHunter Workstation Software - Acquisition for TOF B.01.03 • Run autotune with the G1969-85000 Tune Mix. There are no tune specific methods. • Tune the 6220 in 2GHz extended dynamic range for both positive and negative. • Tune the 6210 in Standard (3200 m/z) mode 1GHz.

  • Page 44: Step 2. Set Up Method Names And Parameters

    Installation Verification Step 2. Set up method names and parameters 1 Create six methods from Default.m for the multimode ESI + APCI LC Demo Sample (p/n G1978-85000), using these method names: • MMCHECKTOF_EI_POS.m • MMCHECKTOF_EI_NEG.m • MMCHECKTOF_CI_POS.m • MMCHECKTOF_CI_NEG.m • MMCHECKTOF_MX_EI POS_CI POS.m •...
  • Page 45 Installation Verification Step 3. Create MMCHECKTOF_EI_POS.m The graphics in this topic differ slightly for MassHunter B.01.03 or higher. For B.01.03, access these tabs from the Acquisition view. • Set the parameters for MMCHECKTOF_EI_POS.m: Figure 30 Acquisition parameters Figure 31 Chromatogram Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 46 Installation Verification Ionization Mode MM-ES Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...

  • Page 47: Mmchecktof_Ei_Neg.m

    Installation Verification Step 4. Create MMCHECKTOF_EI_NEG.m • Set the parameters for MMCHECKTOF_EI_NEG.m: Figure 32 Acquisition Figure 33 Chromatogram Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 48 Installation Verification Ionization Mode MM-ES Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...

  • Page 49: Mmchecktof_Ci_Pos.m

    Installation Verification Step 5. Create MMCHECKTOF_CI_POS.m • Set the parameters for MMCHECKTOF_CI_POS.m: Figure 34 Acquisition Figure 35 Chromatogram Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 50 Installation Verification Ionization Mode MM-APCI Polarity Positive 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...

  • Page 51: Mmchecktof_Ci_Neg.m

    Step 6. Create MMCHECKTOF_CI_NEG.m • Set the parameters for MMCHECKTOF_CI_NEG.m: Figure 36 Acquisition Figure 37 Chromatogram...
  • Page 52 Installation Verification Ionization Mode MM-APCI Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...

  • Page 53: Mmchecktof_Mx_Ei Pos_Ci Pos.m

    Installation Verification Step 7. Create MMCHECKTOF_MX_EI POS_CI POS.m • Set the parameters for MMCHECKTOF_MX_EI POS_CI POS.m. Figure 38 Acqusition Figure 39 Chromatogram Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 54 Installation Verification Ionization Mode MM-ES+APCI Polarity Positive 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2% acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...

  • Page 55: Mmchecktof_Mx_Ei Neg_Ci Neg.m

    Installation Verification Step 8. Create MMCHECKTOF_MX_EI NEG_CI NEG.m • Set the parameters for MMCHECKTOF_MX_EI NEG_CI NEG.m: Figure 40 Acqusition Figure 41 Chromatogram Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 56 Installation Verification Ionization Mode MM-ES+APCI Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Solvents Solvent A 100.0 % (65%MeOH:35%H O + 0.2% acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar...
  • Page 57 Installation Verification Step 9. Run each of the methods created 1 Run each of the methods that you just created. The real time plot below shows the six runs. ESI +APCI NEG 1-Hexanesulfonic acid & 9-Phenanthrol ESI NEG APCI POS APCI NEG ESI +APCI POS Crystal ESI POS...
  • Page 58 Installation Verification 3 View the data in the data analysis program for MM_ESI_Neg. Extract Ion 165-165.4. Record the peak height Example 97,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 59 Installation Verification 4 View the data in the data analys program for MM_APCI_POS. Extract Ion 168-168.4. Record the peak height. Example 140,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 60 Installation Verification 5 View the data in the data analysis program for MM_APCI_NEG. Extract Ion 193-193.4. Record the peak height. Example 640,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 61 Installation Verification 6 View the data in the data analysis program for MM_ ESI_APCI_POS. Extract Ion 372-372.4. Record the peak height. Example: 57,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 62 Installation Verification 7 View the data in the data analysis program for MM_ ESI_APCI_POS. Extract Ion 168-168.4. Record the peak height. Example: 34,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 63 Installation Verification 8 View the data in the data analysis program for MM_ ESI_APCI_NEG. Extract Ion 165-165.4. Record the peak height. Example: 110,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 64 Installation Verification 9 View the data in the data analysis program for MM_ ESI_APCI_NEG. Extract Ion 193-193.4. Record the peak height. Example: 400,000. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 65 Installation Verification Step 10. Calculate the response of Multimode Demo 1 Manually fill in the values in the Multimode Ion Source report. The values in the example report below have been manually entered from the data collected in the runs from the previous steps. This is an example of how to enter the values from the instrument being installed and verified.
  • Page 66 Installation Verification Step 11. Fill out Multimode Report for calculation of peak heights • Use the graphic below to fill out the multimode report for calculation of peak heights. Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide...
  • Page 68 In This Book This book contains information to install, replace, and verify your Agilent G1978A Multimode for 6210/6220 TOF. © Agilent Technologies, Inc. 2008 Printed in USA First Edition, December 2008 *G1978-90060* G1978-90060 Agilent Technologies...

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