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Related Manuals for MGI Complete Genomics DNBSEQ-G800RS
Summary of Contents for MGI Complete Genomics DNBSEQ-G800RS
- Page 1 DNBSEQ-G800RS User Manual For Research Use Only. Complete Genomics, Inc.
- Page 2 About this manual This manual is applicable to Genetic Sequencer (DNBSEQ-G800RS). The manual version is 1.0 and the software version is V1. This manual and the information contained herein are proprietary to Complete Genomics, Inc. (hereinafter referred to as CG), and are intended solely for the contractual use of its customers in the use of the products described herein and for no other purpose.
- Page 3 Revision history Date Version Initial release March 15, 2023...
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Page 4: Table Of Contents
Contents Contents Safety Conventions used in this guide General safety Electrical safety Mechanical safety Components safety Biological safety Symbols Packaging Device Label User manual Device overview Intended use Working principle Sequencer overview Structural composition Basic components Control software Laboratory requirements Site requirements Network requirements Device requirements... - Page 5 Contents Manual Workflow Preparing DNBs Preparing the reagent cartridge and flow cell Reagent cartridge and sequencing flow cell Cleaning cartridge and washing flow cell Preparing the device Powering the device on Logging in to the control software Performing pre-run checks Performing an auto wash Loading the flow cell Cleaning the flow cell satge...
- Page 6 Contents Manual Flow cell stage and flow cell maintenance Introduction of flow cell stage and flow cell Cleaning the flow cell stage and flow cell Cleaning sealing gasket Sequencer maintenance Wash Weekly maintenance Monthly maintenance Annual maintenance Maintaining the software FAQs Storage and transportation Disposal of the device...
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Page 8: Safety
Safety This chapter describes basic safety information about the device. Carefully read and understand the information before use to ensure correct operations, best performance, and personnel safety. Keep this guide at hand for reference at any time. -
Page 9: Conventions Used In This Guide
Safety Conventions used in this guide Conventions used in this guide The following table describes conventions that are used in this guide: Item Description Means compliance with a requirement or it is mandatory for shall compliance with this document Means compliance with a requirement but it is not mandatory for should compliance with this document Used to describe possibility or probability... - Page 10 Safety General safety WARNING y Only CG Technical Support or qualified and trained personnel can unpack, install, move, debug and maintain the device. Incorrect operations can cause altered experimental results or damage to the device. y Do not move the device after CG Technical Support have installed and debugged the device.
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Page 11: Electrical Safety
Safety Electrical safety Electrical safety DANGER y Ensure that the device is properly grounded, and the grounding resistance meets the requirements. Failure to do so may result in altered experimental results, electrical leakage, or even electrical shock. If you have concerns about proper device grounding, please contact CG Technical Support. -
Page 12: Biological Safety
Safety Biological safety Biological safety y Reagents and waste chemicals may cause personal injury through skin, eye, or mucosal contact. Follow the safety standards of your laboratory and wear protective equipment (such as a laboratory coat, protective glasses, mask, gloves, and shoe covers) when using the device. -
Page 13: Device
Safety Symbols Symbol Name Description Indicates that stacking of the crated is not allowed Do not stack and no item shall be placed on top during transport or storage Indicates that the crated shall not be rolled or Do not roll turned over. -
Page 14: Label
Safety Symbols Symbol Name Description Indicates the terminal of a protective Protective earth earth (ground) electrode "ON" (power) Indicates the main power supply is on "OFF" (power) Indicates the main power supply is off Fuse specification Indicates the fuse specification T16AH250V USB 3.0 port USB connection... -
Page 15: User Manual
Safety Symbols Symbol Name Description Indicates that waste electrical and electronic equipment must not be disposed of as unsorted muni c ipal waste and must WEEE symbol be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment. -
Page 16: Device Overview
Device overview This chapter describes the intended use, working principle, and structural composition of the device. -
Page 17: Intended Use
Device overview Intended use Intended use This device is a sequencing instrument that measures optical and electronic signals of the reporting molecules, which decode the sequence information of a DNA or RNA fragment. This is accomplished through the use of instrument specific reagents, flow cells, imaging hardware, and data analysis software. -
Page 18: Sequencer Overview
Device overview Sequencer overview The following figure demonstrates how to load DNBs: DNBs Flow cell Decorated sites evenly spread on the flow cell Each site contains a single DNB Figure 2 Loading DNBs The DNBs and sequencing reagents are pumped into the sequencing flow cell through the device’s liquid delivery system. -
Page 19: Basic Components
Device overview Sequencer overview Component Description Connects the flow cell to fluidics lines and controls the Flow cell stage temperature of the flow cell. Provides the gas-liquid support that is required for the Gas-liquid system biochemical reaction. Electric control system Controls the electric system. - Page 20 Device overview Sequencer overview Name Description Displays the current status of the device: y Green: the device is running. y Blue: the device is in standby status. Status indicator y Yellow: a warning appears, but the device keeps running. y Red: an error occurred. Supporting feet Supports the main unit to ensure stability.
- Page 21 Device overview Sequencer overview Back view Figure 4 Back view Name Description Ventilation outlet Ventilates the device. Wheel Used for moving the device. Used to connect to USB devices such as the keyboard, USB 3.0 port mouse, and scanner. UPS port Used to connect to the UPS power supply.
- Page 22 Device overview Sequencer overview Name Description Network port Used to connect to the network. Left view Figure 5 Left view Name Description Holds flow cells and controls the temperature for Flow cell compartment biochemical reactions.
- Page 23 Device overview Sequencer overview Name Description Used to observe the status of the fluidics system and Window negative pressure gauge through the window. Ventilation inlet Exhausts air from the device. Co n n e c t s t h e wa ste l eve l s e n s o r i n t h e wa ste Level sensor port container.
- Page 24 Device overview Sequencer overview Right view Figure 6 Right view Name Description Fuse seat Used to install fuses. P o w e r s u p p l y Connects the device to the power supply. port Power cord hook Prevent power cord from moving. Powers the device on or off.
- Page 25 Device overview Sequencer overview Flow cell compartment Figure 7 Flow cell compartment Name Description Press the button of the flow cell compartment Flow cell compartment door door to open the door. Holds, moves the flow cell B, and controls the Flow cell stage B temperature that is required for biochemical reaction.
- Page 26 Device overview Sequencer overview Name Description Press to activate the vacuum for attachment or Flow cell attachment button A release of the flow cell A. Press to activate the vacuum for attachment or Flow cell attachment button B release of the flow cell B. Reagent compartment Figure 8 Reagent compartment Name...
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Page 27: Control Software
Device overview Sequencer overview Name Description Holds the reagent cartridge at appropriate temperatures: y Reagent cartridge A is placed on the left. It provides the required reaction mixture for Reagent compartment flow cell A. y Reagent cartridge B is placed on the right. It provides the required reaction mixture for flow cell B. - Page 28 Device overview Sequencer overview 1. In the main interface, select , and select Library to check the detailed self- test results that are recorded in the library. 2. Follow the on-screen instructions. 3. Perform the self-test again: , select Maintenance > Self-test. ƒ Select ƒ...
- Page 29 Device overview Sequencer overview The following table describes the function of each area or button in the main interface: Name Description Notification area Indicates warnings, errors, date, and time. Flow cell A Indicates the status of flow cell A and provides wash and operation area sequence options.
- Page 30 Device overview Sequencer overview Item Description Negative pressure is normal. Negative pressure is beyond the normal range. Basecalling is connected. Errors occur in the basecalling connection. The basecall software is processing image data. This icon is dynamic. Select to view more status icons. Fluidics pressure.
- Page 31 Device overview Sequencer overview Item Description Indicates the temperature of device. Real-time temperature appears on the left of the icon. Indicates the temperature of reagent cartridge. Real-time temperature appears on the left of the icon. Indicates the humidity of device. Real-time humidility appears on the left of the icon.
- Page 32 Device overview Sequencer overview The following table describes the function of controls in the interface: Item Description Select to exit the system settings interface and return to the previous Back interface. Select to change the language of the software. Restart the device to Language apply the changes.
- Page 33 Device overview Sequencer overview Item Description y Select a data type and export the data to the specified directory of the external storage device. Export data y Select Uploading data and upload the data to the specified server. Select to export scripts to the external storage device Script setting or import scripts from the external storage device.
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Page 34: Laboratory Requirements
Laboratory requirements This chapter describes the requirements for the laboratory, the network, the power supply and so on. -
Page 35: Site Requirements
Laboratory requirements Site requirements Site requirements DANGER y Ensure that the laboratory floor is level and with a gradient of less than 1/200. y Ensure that the laboratory is free of dust, corrosive and flammable gas, and heat and wind sour. -
Page 36: Network Requirements
Laboratory requirements Network requirements Network requirements WARNING y If necessary, contact the technical support to obtain or change the user name and password of the computer and the device. y The control software provides two different types of user accounts. For detailed access authorization, contact the technical support. -
Page 37: Device Requirements
Laboratory requirements Device requirements Item Description We have pre-installed the antivirus software. Software security If you need to upgrade the antivirus software, contact the technical support in advance. y Network ports: connect to the network. y USB type A ports: connect to the scanner. Data and device port y USB ports: connect to external USB devices, such as the keyboard, mouse and scanner,... -
Page 38: Power Supply Requirements
Laboratory requirements Power supply requirements The following table describes indicator status on the label: Label Indicator status Description Indicates that the device is intact and no Remains strong collision occurs during transportation, unaffected or the intensity does not exceed the limit. Anti-shock label Indicates that the device might not be intact and that a strong collision occurs during... -
Page 39: Peripheral Device Requirements
Laboratory requirements Peripheral device requirements Item Description 1200 VA, the available current should be not less Rated power than 16 A. Transient over-voltage category Grounding resistance < 4 Ω Peripheral device requirements Before using the device, prepare the following peripheral items: Recommended Device Remarks... -
Page 40: Sequencing
Sequencing This chapter describes the sequencing workflow, sequencing and analysis, and post-sequencing procedures by using the flow cell A operation area as an example. Read and follow the instructions to ensure correct operations.
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