Related Manuals for TOSOH BIOSCIENCE TSKgel NH2-100 3mm
Summary of Contents for TOSOH BIOSCIENCE TSKgel NH2-100 3mm
- Page 1 Rev. CO067000 Packed Columns for Normal Phase Chromatography - 100 3 μm TSKgel NH INSTRUCTION MANUAL...
- Page 2 Safety Precautions To help protect you and/or your property from potential damage, please read this manual thoroughly before using the product. [Notation Conventions] Explanation Notation Indicates a hazard with a medium level of risk which, if WARNING not avoided, could result in death or serious injury. Indicates a hazard with a low level of risk which, if not CAUTION avoided, could result in minor or moderate injury.
- Page 3 Precautions: Shipping Solvents • Move the person to an area with fresh air. First Aid Inhalation Rinse the mouth with plenty of water. • Call for medical attention immediately. • Wash exposed area with plenty of soap and water. Skin exposure •...
- Page 4 Precautions: Packing Material • Move the person to an area with fresh air. Inhalation First Aid Rinse the mouth with plenty of water. • Call for medical attention immediately. • Wash exposed area with plenty of soap and water. Skin exposure •...
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Page 5: Table Of Contents
Table of Contents ……………………………………………………… 1 01. General Information 02. Unpacking …………………………………………………………………… 1 03. Column Parts ……………………………………………………………… 1 04. Column Installation ………………………………………………………… 2 05. Column Maintenance ……………………………………………………… 3 06. Solvent Selection and Preparation ……………………………………… 3 07. Flow Rate …………………………………………………………………… 4 08. Temperature ………………………………………………………………… 5 ………………………………………………………... -
Page 6: General Information
Published, February 2009 1. General Information TSKgel NH -100 3μm packed columns have been optimized for high performance NPC and HILIC. Please read this INSTRUCTION MANUAL carefully and use the column as recommended in order to make effective use of its high performance. 2. -
Page 7: Column Installation
2) Guard column 3.2 mm(I.D.)× 1.5 cm(L) Guard column Cartridge holder (Outlet) Cartridge holder (Inlet) Column-extracting tool 2.0 mm(I.D.)× 1 cm(L) Cartridge holder (Inlet) Guard column Cartridge holder (Outlet) Figure 3 Column Parts (2) 4. Column Installation (1) Confirm the correct product name is listed on the column label. (2) Each column is equipped with a union nut enabling a connection to a 1/16 O.D. -
Page 8: Column Maintenance
(8) Start pumping the solvent at a flow rate less than one-half of the final flow rate. Avoid a sudden pressure surge to the column. (9) The columns are very sensitive to pressure pulsing. A pulseless pumping system should be used. (10) The columns should be equilibrated before use by allowing at least 10 column volumes of solvent to pass through the column. -
Page 9: Flow Rate
solvent should be replaced with an appropriate mobile phase for analysis. When salt-containing buffers are used as a mobile phase, purge the column for at least 1 h with salt solution containing 5 % of a water-soluble organic solvent (e.g., 200 mmol/L ammonium formate/ acetonitrile= 95/5, v/v) to exchange the counter- anion associated with the amino group on the packing material. -
Page 10: Temperature
Table 1 Maximum Pressure Maximum Column Size Pressure Part No. Type mm(I.D.)×cm(L) (MPa) -100 3 μm 0021967 TSKgel NH 2.0×5 0021968 〃 2.0×15 0021969 〃 4.6×5 0021970 〃 4.6×15 8. Temperature The column should be operated in a temperature range of 10 - 50 ℃. 9. -
Page 11: Guard Column
N=5.54(Ve/W where: Ve : Elution time : Width of peak at half-height : Peak height : Number of theoretical plates/column (2) Asymmetry factor (As) The asymmetry factor is calculated according to Figure 4 and the following equation: 1/10h Injection Figure 4 Calculation of Asymmetry Factor As=b/a (3) The N and As should be measured with an instrument with small dead volume. - Page 12 11-1 Effect of Guard Column Installation (1) Contamination of the analytical column can be prevented by the removal of adsorptive or insoluble materials in the sample. (2) Pressure shock, due to pump pulsation, to the analytical column should be avoided. 11-2 Type and Selection of Guard Columns Guard columns specifications are shown in Table 2.
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Page 13: Troubleshooting
If a mixture of water or aqueous acetate solution with water-soluble organic solvents (e.g., acetonitrile) are used as a mobile phase, the guard column should be replaced after approximately 70 h of usage (for 4.6 mm(I.D.) columns) or 40 h of usage (for 2.0 mm(I.D.) columns). -
Page 14: Column Cleaning Solutions
performance according to Section 10. If the guaranteed specifications in Table 4 can not be obtained, contact a local TOSOH representative within 30 days. Note that the column lifetime is not guaranteed. Table 4 Guaranteed Specifications Number of Column Size Asymmetry theoretical plates Part No. - Page 15 Shiba-Koen First Bldg. 3-8-2 Shiba, Minato-ku, Tokyo 105-8623, Japan Phone: +81-3-5427-5180 Fax: +81-3-5427-5220 Web site: http://www.separations.asia.tosohbioscience.com/ HPLC database: www2.tosoh.co.jp/hlc/hlcdb.nsf/StartE?OpenForm TOSOH BIOSCIENCE GmbH TOSOH BIOSCIENCE LLC 3604 Horizon Drive Suite 100, Zettachring 6, 70567 Stuttgart, Germany King of Prussia, PA 19406, USA...
- Page 17 Rev. CO067000A0 Published in February, 2010 Supplemental Information related to TSKgel NH -100 3μm columns INSTRUCTION MANUAL for TSKgel NH -100 DC 3μm (4.6 mm(I.D.), 5 cm(L)) To help protect you and/or your property from potential damage and ensure personal safety, please read the “Precautions”...
- Page 18 4. Column Installation (13) To directly connect the TSKgel NH -100 DC 3μm column to another HPLC column, finger-tighten the connection between the outlet of the TSKgel NH -100 DC 3μm column and the inlet of the second column, and then tighten with appropriate wrenches until you feel some resistance.
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