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Summary of Contents for TOSOH BIOSCIENCE TSKgel HPLC Column
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Page 1: Table Of Contents
ANALYSIS TSKgel HPLC Column ® Instruction Manual Important! Do not throw away! Thank you for purchasing a TSKgel liquid chromatography column! Table of Contents What to do first Installation Column protection Quality control Operating conditions Troubleshooting Return policies Additional help... -
Page 2: What To Do First
30 days after receiving the column. Returns can only be accepted if the column is shipped back in the Tosoh Bioscience column box and is accompanied by the original data sheet and the Tosoh Bioscience column tag (see „Return Policy“... - Page 3 Alternatively, you can use 10·32 (1/16“) fingertight fittings that are available from chromatography supply houses. In either case, make sure to insert the capillary tubing fully into the column end fitting before setting the ferrule by tightening the nut. Repeat to make the connec- tions at the other end of the 1/16“...
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Page 4: Column Protection
If the column inlet fitting appears dry, we recommend that you first disconnect the bottom cap and hook up the column exit to the injector. Then slowly start the flow in this reversed flow direction until a few drops of mobile phase exit from the column. - Page 5 Ferritin Sigma-Aldrich F4503 y-Globulin Sigma-Aldrich G5009 Lysozyme Sigma-Aldrich L6876 d-Mannitol Sigma-Aldrich M4125 Ovalbumin Sigma-Aldrich A2512 Polyethylene oxide (kit) Tosoh Bioscience 05773 Polystyrene (kit) Tosoh Bioscience 06476 Ribonuclease A Sigma-Aldrich R5503 Thyroglobulin Sigma-Aldrich T1001 Trypsin Sigma-Aldrich T7409 Trypsin inhibitor Sigma-Aldrich T9003...
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Page 6: Operating Conditions
Efficiency (N) N = 5.54 (V = elution volume at the peak maximum ½ h = width of peak at half height Asymmetry factor (AF) AF = b/a AF is measured at 10% of the peak height. Resolution (R = elution volume of first = 2(V and second... - Page 7 For example, a low pH salt solution will remove basic proteins. Organics will remove hydrophobic proteins. Chaotropic agents will remove strongly adsorbed materials (e.g., via hydrogen bonding). For columns or column types not listed below, please contact Tosoh Bioscience Technical Service for the appropriate solutions.
- Page 8 Cleaning solutions Generally, 3-5 column volumes (CV) of cleaning solution is sufficient. Rinse well with 3-5 CV of distilled, deionized water between each solution. Only use chaotropic agents when neutral salt or organic has not improved performance characteristics. SW and SW , UP-SW, Super SW 1.
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Page 9: Troubleshooting
As a last resort, buffered solutions of SDS (0.1%), urea (8 M) or guanidine (6 M) can be used to clean the column. We only recommend this method if all other methods fail. Storage Consult the OCS sheet for the recommended storage solvent and storage tem- perature or temperature range for this column. - Page 10 If not, clean and/or replace the end fitting (available from Tosoh Bioscience) as described below. To clean or replace the end fitting, use a vise to carefully remove the old fitting from the top of the column, making sure not to disrupt the gel packing at the col- umn top.
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Page 11: Return Policies
If a product is authorized for return for reasons other than Tosoh Bioscience’s error or because of a product defect, there will be a restocking charge of 25 Euro or 10% of the list price, whichever is greater. - Page 12 Im Leuschnerpark 4, 64347 Griesheim, Germany Phone: +49 6155 70437-00 Fax: +49 6155 83579-00 Order Phone: +49-6155 70437-30 Order Fax: +49-6155 83579-01 info.tbg@tosoh.com, www.tosohbioscience.de M19L12A...
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