Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for TOSOH BIOSCIENCE Phenyl-5PW
Summary of Contents for TOSOH BIOSCIENCE Phenyl-5PW
- Page 1 HPLC HIC Column TSKgel Phenyl-5PW, Ether-5PW INSTRUCTION MANUAL...
- Page 2 Safety Precautions To help protect your property from potential damage and ensure personal safety, please read this manual thoroughly before using the product. ［Notational Conventions］ Notation Explanation WARNING Alerts the user to the potential for serious injury or death. CAUTION AIerts the user to the potential for damage to hardware or bodily harm. WARNING ■ Keep away from fire. Take proper precautions when using flammable solvents. There is the potential for fire, explosion, or poisoning. CAUTION ■ Use only in well ventilated areas. In case of insufficient ventilation, flammable and toxic solvents can cause fire, explosion, or poisoning. ■ Do not spill solvents. Spillage and leakage can cause fire, electric shock, poisoning, injury, and corrosion. When cleaning up a spill, wear appropriate protective gear. ■ Wear eye protection and protective gloves. Organic solvents and acids should not come in direct contact with the skin. ■ Handle package with care. Inappropriate handling may cause rupturing and splattering. ■ Only use this product as intended. This product is for separation and purification, do not use for any other purpose. ■ Confirm compounds are safe. Check that obtained compounds and solutions after separation and purification are safe. ■ Proper disposal. Dispose of in accordance with local laws and regulations. NOTE ■ Keep this manual for future reference. ...
-
Page 3: Table Of Contents
Table of Contents 1 . Introduction …………………………………………………………… 1 2 . Unpacking …………………………………………………………… 1 3 . Installation …………………………………………………………… 1 4 . Column Storage ……………………………………………………… 3 5 . Sample and Eluent Preparation ……………………………………… 3 6 . Flow Rates …………………………………………………………… 4 7 . Temperature …………………………………………………………… 5 8 . Guard Column ………………………………………………………… 5 9 . Column Efficiency …………………………………………………… 6 10. Troubleshooting ……………………………………………………… 7 11. Quality Specifications and Warranty ………………………………… 7... -
Page 4: Introduction
1. Introduction TSK-GEL HIC colummns are prepacked columns for the separation of sample components according to surface hydrophobicity. The separation mechanism is similar to that of reversed phase. These columns were designed for analytical and preparative separation of proteins. We have both steel column and glass column. Especially glass column is biocompatible type which consists of a high precise glass tube with lastic end fittings. This Instruction Manual contains crucial information on how to care for and use these columns in the proper manner, so as to make most effective use of their high performance capabikities. - Page 5 Operating the column with the flow in the reverse diraction for a long time will cause degradation of column performance. Outlet Inlet FLOW End fitting TY PE End Plug C. No. Name Plate Fig. 2 Steel Column Parts Inlet Outlet End fitting End Plug Fig. 3 Glass Column Parts 3-3 Prevention of Bubbles Be careful not to admit any bubbles into the column during its installation or removal from the equipment. Always remove all bubbles from all pipings before installing the column. Admitting bubbles into the column will cause degradation of its performance through the occurrence of channeling, etc. 3-4 Prevention of Pulsatory Flow This type of column is easily affected by pulsatory flow of the solvent. Preferably, a pump with no fluctuation should be used. If a pump with pulsation must be used, connect a pulse damper (accumulator) to the outlet side of the pump in order to compensate for the pulsation. The damper must be highly resistant to corrosion. − ２ −...
-
Page 6: Column Storage
4. Column Storage Store the columns at constant temperature in the range of 4 ℃ to 30 ℃. Don t store these columns below 4 ℃ not to freeze the solvent in the column. Avoid exposing the columns to direct sunlight, and store the columns in a place safe from corrosive gases. If the column is being used daily, you need not remove the column from the equipment and the buffer may be left in the column overnight, as long as the buffer salt has good solubility. When the columns are to be stored for a few days, remove the column from the instrument and seal both ends with the end plugs. For long-term storage, the column must be protected from growth of microorganisms. -
Page 7: Flow Rates
0.4-0.8 mL／ min. The flow rates and pressure drops for these columns are suggested below : Table 1 Recommended flow rates Recomended Max. Pres- Column Max. Cat. Flow Rate sure Drop Types Dimensions Flow Rate (mmID×cm) (mL／min) (mL／min) (MPa) 〈Steel Column〉 07573 phenyl-5PW 7.5× 7.5 0.5−1.0 07656 phenyl-5PW 21.5×15.0 4.0−6.0 〈Glass Column〉 13063 phenyl-5PW Glass 5.0× 5.0 0.5−0.8 08804 phenyl-5PW Glass 8.0× 7.5 0.5−1.0 14018 phenyl-5PW Glass 20.0×15.0 4.0−6.0... -
Page 8: Temperature
In such case the original column efficiency can be maintained by connecting a guard column before the column and replacing it if efficiency becomes degraded by the adsorption of material to the guard column. For mazimum insurance against such problems, a guard column should be used as much as possible. We prepared the Guard column kit as the guard columns for TSKgel Phenyl-5PW and Ether-5PW. Please refer to INSTRUCTION MANUAL for TSKguardgel for HPLC . Table 2 Guard Columns Types Applied Columns Cat. No. -
Page 9: Column Efficiency
9. Column Efficiency The number of theoretical plates of a column (N), the asymmetry factor (As) and their chromatographic conditions are shown in the Inspection Data. 9-1 Method of Calculating the Number of Theoretical Plates 1/2h Injection Fig. 4 Method of Calculating the Number of Theoretical Plates The number of theoretical plates of a column (N) is calculated by the half peak width method shown in Fig.4 and the following equation. N ＝ 5.54(Ve ／ W 1／2 : Elution volume : Half width value of peak 1／2 : Peak height 9-2 Method of Calculating the Asymmetry Factor 1/10h Injection Fig. 5 Method of Calculating the Asymmetry Factor The asymmetry factor of column (As) is calculated by the 1 ／10 h method. As ＝ b ／ a 9-3 Dead Volume If the dead volume of the equipment or the injection volume of a sample solution becomes too large, the number of theoretical plates may decrease. -
Page 10: Troubleshooting
10. Troubleshooting When using TSKgel columns, some problems can be avoided by following these instructions. But the problems (such as those due to columnn life, adsorptive materials, production of air bubbles, dried gel, or frozen solvent) can not be corrected once they occur, so care should be taken in handling these columns. 10-1 Clogging of the End Fitting In case the pressure-drop increases or the flow rate decreases, the end fitting should be cleaned by reversing the flow through the column. If the clog cannot be removed, prepare a new end fitting and replace the old end fitting ... - Page 11 Glass Column 8.0× 7.5 Glass Column 20.0×15.0 ＊ Detector : UV (280 nm) 11-2 Quality Specifications Column No. of Theo- Asymmetry Cat. Dimensions retical Plates Factor Types (mmID×cmL) (N／Column) (As) 〈Steel Column〉 07573 TSKgel Phenyl-5PW 7.5× 7.5 ≧1,000 1.0−2.0 07656 TSKgel Phenyl-5PW 21.5×15.0 ≧3,000 0.8−1.6 08641 TSKgel Ether-5PW 7.5× 7.5 ≧1,000 1.0−2.0 08642 TSKgel Ether-5PW 21.5×15.0 ≧3,000 0.8−1.6 〈Glass Column〉...
- Page 12 Shiba-Koen First Bldg. 3-8-2 Shiba, Minato-ku, Tokyo 105-8623, Japan Phone: +81-3-5427-5180 Fax: +81-3-5427-5220 Web site: http://www.separations.asia.tosohbioscience.com/ HPLC database: www2.tosoh.co.jp/hlc/hlcdb.nsf/StartE?OpenForm TOSOH BIOSCIENCE GmbH TOSOH BIOSCIENCE LLC 3604 Horizon Drive Suite 100, Zettachring 6, 70567 Stuttgart, Germany King of Prussia, PA 19406, USA...
Need help?
Do you have a question about the Phenyl-5PW and is the answer not in the manual?
Questions and answers