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ARA G-FL User Manual

Laboratory fermenter system
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User manual
ARA G-FL
Laboratory Fermenter System

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Summary of Contents for ARA G-FL

  • Page 1 User manual ARA G-FL Laboratory Fermenter System...
  • Page 2 USER MANUAL │ ARA G-FL Product Code ARA G-FL Manufacturer Hanil Scientific Inc. 16 Arayuk-ro, Gimpo-si, Gyeonggi-do, 10136, KOREA www.ihanil.com Package 1 set of Main Controller, Vessel Assembly, Motor, and Accessories The contents of the user manual are subject to change without notice.

  • Page 3: Table Of Contents

    USER MANUAL │ ARA G-FL CONTENTS 1 Safety Precautions ------------------------------------------------- 5 1.1 General Safety Instructions --------------------------------- 5 1.2 Precautions for use ----------------------------------------- 6 2. Check the packaging and components -------------------------- 7 2.1 Check the packaging ---------------------------------------- 7 2.2 Check the components ------------------------------------- 7 2.3 Component List --------------------------------------------- 7...
  • Page 4 USER MANUAL │ ARA G-FL 6.2 Touch monitor-------------------------------------------------- 21 7. Control Screen Configuration ---------------------------------------- 21 7.1 Main screen ---------------------------------------------------- 21 7.2 Product Information screen ------------------------------------23 7.3 Control Screen ------------------------------------------------- 23 7.4 Data Log -------------------------------------------------------- 29 7.5 Calibration ------------------------------------------------------ 30 1) pH Calibration --------------------------------------------- 30...

  • Page 5: Safety Precautions

    USER MANUAL │ ARA G-FL 1. Safety Precautions 1.1 General safety instructions Before supplying power to use the bioreactor, remove all packaging materials used to prevent damage during transport. Check if any of the necessary parts and devices are missing Main Controller and Vessel should be installed flat, so they do not shake.

  • Page 6: Precautions For Use

    USER MANUAL │ ARA G-FL 1.2 Precautions for use Since the bioreactor is used in combination with several components, it is necessary to know the precautions for the use of each component. Please check the following precautions one more time before using.

  • Page 7: Check The Packaging And Components

    USER MANUAL │ ARA G-FL 2. Check the packaging and components 2.1 Check the packaging Upon receipt of the product, carefully check the condition of the box to make sure that the outside of the box has not been damaged during transportation.

  • Page 8: Product Overview

    USER MANUAL │ ARA G-FL 3. Product Overview 3.1 Summary • Easy-to-operate 8-inch touch screen • Compact structure that enables culture experiments in a small laboratory space • Vessel size of various culture volumes from 1.5 to 14 L •...

  • Page 9: Product Composition

    USER MANUAL │ ARA G-FL 3.2 Product Composition 1) Main Controller Front View Side View Top View Touchscreen Pump Sensor Port Rotameter Power Socket & Switch (with Fuse) Power Switch Micro SD Slot Analog Output Analog Input Heating Plate Socket...
  • Page 10 USER MANUAL │ ARA G-FL 2) Vessel Assembly Condenser Motor Top Plate (4ea) 고정너트 PH,/ DO Sensor Sampling Pipe Feeding Pipe Sparger Nipple Cold Finger Temp Sensor Inoculum Handle (Option) Inoculum 손잡이 Foam Breaker 4P Feeding Agitator Shaft Port Impeller...

  • Page 11: Specification

    USER MANUAL │ ARA G-FL 3.3 Specification 1) Main Controller Main Controller 8” Touch Screen IPS LCD capacitive Display Digital display, Graph, incensement 0.1 ℃ Temperature Range 8 ~ 70℃ (±0.5℃) Sensor PT100 Drive Direct drive / Capacity select (60W: ∼ 7 L, 150w: 10 L or more)
  • Page 12 USER MANUAL │ ARA G-FL 2) Vessel Assembly 2. Single Round 3. Double Vessel 1. Single Vessel 4. Bowl Vessel Vessel (3,5,7L) (1.5L, 3L, 5L 7L, 10L) (10, 14L) (3L, 5L) Working Vol.(L) Diameter(mm) Height(mm) Single Top Plate Dia. (mm) pH/DO Sensor Length (mm) Temp.
  • Page 13 USER MANUAL │ ARA G-FL 3) Impeller and Foam Breaker Type Diagram Description Dia. (mm) Rushton Rushton turbine impeller is a flat bladed turbine type that has low resistance and can rotate at high speed, which is why it is generally 75, 90, 110 used the most.

  • Page 14: Product Assembly And Installation

    USER MANUAL │ ARA G-FL 4. Product assembly and installation Before installation The surface which the product will be installed must be flat and hard. When installing the product on a workbench, it must be able to support the weight of the product.
  • Page 15 USER MANUAL │ ARA G-FL 3) Installing the 4P Feeding Port ① Insert 4 Feeding Pipes (Φ4) into each of the 4 holes in the rubber stopper. ② Insert the rubber stopper with the feeding pipe into the 4P Feeding Port of the Top Plate and tighten the rubber cap nut.

  • Page 16: Heating Plate Assembly And Water Line Connection

    USER MANUAL │ ARA G-FL 8) Top plate assembly and sensor installation ① Insert the gasket on the upper part of the vessel. ② After installing the Top Plate on the Vessel, tighten the Top Plate fixing nut. Tighten the Top Plate retaining nuts diagonally.

  • Page 17: Installing The Motor

    USER MANUAL │ ARA G-FL Installing the Motor ① Insert the motor by aligning the coupling groove in the center of the TOP Plate. ② Tighten the motor fixing bolt by turning it clockwise. Silicone Tube and Cable Connection ① Connect the silicone tubes (Acid, Base, Feed and Anti-foam tube) to the Peristaltic Pump as shown in the figure below.

  • Page 18: Air Connection

    USER MANUAL │ ARA G-FL Air Connection ① Connect the air outlet of the air pump to the air in port on the back of the main controller with an air hose. ② Connect the Air Out Port on the back of the Main Controller and the Sparger Nipple on the TOP Plate with an air hose.

  • Page 19: Gas Mixer Connect (Option)

    USER MANUAL │ ARA G-FL Gas Mixer Connection (Option) 4.7.1 Gas Mixer composition Front View Top View Side View Front view Back view 4.7.2 Installing Gas Mixer (2 gas) Connect the gas (N2, O2, CO2, Air, etc.) supply port, Gas1, and Gas2 port to the back of the gas mixer with an air hose (Φ4).

  • Page 20: Disassembly And Cleaning

    USER MANUAL │ ARA G-FL 5. Disassembly and cleaning Disconnecting the motor Disconnect the motor power cable from the Main Controller. Loosen the motor fixing bolt by turning it counterclockwise, then lift the motor in the vertical direction to remove.

  • Page 21: Control Screen Overview

    6 Control screen overview Feature introduction ARA G-FL product is a multifunctional product that can be used for culturing various substances such as microorganisms, plant cells, and animal cells, and can be fermented in various ways such as batch, fed-batch, and continuous. This control system controls pH, temperature, agitation speed, and DO.
  • Page 22 USER MANUAL │ ARA G-FL Sensor: Displays the current value and set value of the sensor. (The graph displays the current value and the value at the bottom of the graph displays the setting value. You can change the setting value by touching the setting area.) Motor: Displays the motor's current RPM and set RPM.

  • Page 23: Product Information Screen

    7.2. Product Information screen • Wi-Fi Setting Touch ARA CI at the bottom of the product information screen to move to the Wi-Fi setting screen. After setting Wi-Fi, touch the Back button [◁] at the bottom to return to the previous screen 7.3.
  • Page 24 USER MANUAL │ ARA G-FL pH Stat If you select the [pH Stat] menu on the [Fed Batch Setup] screen, the following is displayed. [Set Point]: The pH setting value. When touched, a pop-up window to set the pH is displayed.
  • Page 25 USER MANUAL │ ARA G-FL DO Stat If you select the [DO Stat] menu on the [Fed Batch Setup] screen, the following is displayed. [Set Point]: When touched, a pop-up window to set DO. is displayed. [Active Level]: If the current DO. value is higher than the set value, the feed pump operates.
  • Page 26 USER MANUAL │ ARA G-FL User Feeding If you select the [User Feeding] menu on the [Fed Batch Setup] screen, the following is displayed. [Current Step (state)]: Displays the currently executing step number. [Continue Cycle]: If this item is checked, it repeats the operation within the set section.
  • Page 27 USER MANUAL │ ARA G-FL Not Use If [Not Use] is selected on the [Fed-Batch Setup] screen, the feed pump does not operate. This mode applies to batch culture. DO Step Control Setup If you touch the [DO Step Control Setup] menu on the [Control] menu screen, the DO Sensor value screen will be displayed allowing step-by-step control of the stirrer speed.
  • Page 28 USER MANUAL │ ARA G-FL Pump Mode Setup A screen to change the metering pump operation mode is displayed. [ACID Pump Mode]: Select ACID pump operation. [BASE Pump Mode]: Select the BASE pump operation. [FEED Pump Mode]: Select the FEED pump operation.

  • Page 29: Data Log

    USER MANUAL │ ARA G-FL 7.4. Data Log This is a menu that allows you to designate data log items and change the settings of the graph that displays the sensor current value. If you touch the [Data Log] menu on the main screen, the [Data Log Setup] and [Graph Trend] menu is displayed.

  • Page 30: Calibration

    USER MANUAL │ ARA G-FL Graph Trend If you select the [Graph Trend] menu, it is displayed as shown below. [Interval Time]: Set the time interval for displaying each sensor value on the Graph View screen. [OK]: Complete the setting.
  • Page 31 USER MANUAL │ ARA G-FL If you touch the [pH Calibration] key on the [pH Calibration] menu screen, the following is displayed. pH calibration feed button After calibration View area [pH][Temp][mV]: Displays the current measured value. It displays the measured pH value, temperature value, and volt value at the same time.
  • Page 32 USER MANUAL │ ARA G-FL ③ The system automatically calibrates, and the progress bar displays the progress of the calibration. ④ When calibration is complete, remove the sensor from the solution, rinse with distilled water, and dry the sensor outer wall with absorbent paper.

  • Page 33: Do Calibration

    USER MANUAL │ ARA G-FL DO Calibration If you touch the [DO Calibration] key on the [Calibration] menu screen, you can select [DO Calibration] and [Cal Status]. Touching the [DO Calibration] key on the [DO Calibration] menu screen displays the following display.
  • Page 34 USER MANUAL │ ARA G-FL ① Before starting DO calibration, stop the operation of the fermenter and set the stirring speed to the value when fermenting. (You can set it by touching the motor image on the main screen.) Then, prepare nitrogen gas for zero calibration and perform Sat calibration.

  • Page 35: Temp Calibration

    USER MANUAL │ ARA G-FL Temp Calibration If you touch the [Temp Calibration] key on the [Calibration] menu screen, the following is displayed. [Present Value]: Displays the current temperature measurement. [Set Temperature Input]: Set the temperature value to be corrected. (Enter the value measured by the standard sensor.)

  • Page 36: Pump Calibration

    USER MANUAL │ ARA G-FL Pump Calibration If you touch the [Pump Calibration] key on the [Calibration] menu screen, you can select [ACID Pump], [BASE Pump], [FEED Pump], and [FOAM Pump]. (Each pump calibration method is all same.) [Active Time Input]: The time the pump operates during calibration.

  • Page 37: Orp Calibration

    USER MANUAL │ ARA G-FL ⑥ The pump stops automatically after operating for the entered time. ⑦ Enter the amount of liquid in the measuring cylinder in the [Volume] field. ⑧ The system automatically calculates the flow rate of the pump and displays it on the screen.

  • Page 38: Graph

    USER MANUAL │ ARA G-FL 7.6. Graph It is a screen that displays the current value of each sensor as a graph. (After attaching the ORP sensor, if you set the ORP sensor to use in the “12.1.1. Sensor Configuration” menu, the ORP value will also be shown in a graph.)

  • Page 39: Setting

    USER MANUAL │ ARA G-FL 7.7. Setting A login is required to change various settings, and it proceeds in the following pop-up window. If you have not added a user, enter an available user ID/PW initially and enter the setting menu.

  • Page 40: System Configuration

    USER MANUAL │ ARA G-FL System Configuration Touch the [System Configuration] key on the [Settings] menu screen to display the [Sensor Configuration], [Gas Configuration], and [Alarm Configuration] menu. Sensor Configuration If you touch the [Sensor Configuration] menu in the [System Configuration] menu, it is displayed as shown below.
  • Page 41 USER MANUAL │ ARA G-FL Gas Configuration If you touch the [Gas Configuration] menu in the [System Configuration] menu, the following is displayed. [Use Gas-Mixer]: Select when using Gas-Mixer. (It is set to “Disabled” by default.) • Oxygen Type: When using a Gas-Mixer, select one of O2 / Air for the first pump.

  • Page 42: Advanced Configuration

    USER MANUAL │ ARA G-FL Advanced Configuration If you touch the [Advanced Configuration] key on the [Settings] menu screen, [pH Configuration], [DO Configuration], [Temp Configuration] menu can be selected. pH Configuration You can connect by selecting the [pH Configuration] menu from the [Advanced Configuration] menu.
  • Page 43 USER MANUAL │ ARA G-FL • General Mode: Always keep the same pump operation for pH control. • Special Mode: This is a mode for detailed pH control by making the pump operation for pH control longer in the section beyond the Exact Range and shorter in the Exact Range.
  • Page 44 USER MANUAL │ ARA G-FL Temp Configuration If you select the [Temp Configuration] menu from the [Advanced Configuration] menu, it is displayed as shown below. [Method]: It sets which product (Plate or Chiller) is used for temperature control. [Proportional Const]: Set the proportional value for temperature control. Adjust the temperature control speed.

  • Page 45: User Setting

    USER MANUAL │ ARA G-FL 7.8. User Setting It is a menu to add and delete users and to set user-related functions. When entering the menu, see “6.7. Login is required in the same way as entering the “Settings” menu. (For login, refer to “6.7 Settings”.)

  • Page 46: Display On/Off

    [CANCEL]: Cancels the setting.  If you change Remote Control to “Enable”, the IP address of G-FL is displayed on the right side of the Version display area at the top of the screen. (The IP address is used by the G-FL remote control PC program.) ...

  • Page 47: Use Of Gas Mixer (Option)

    O2 (or AIR) / N2/ CO2 gas in addition to the pump (ACID / BASE / FOAM / FEED) connected to G-FL. To connect and use a Gas-Mixer to G-FL, you need to change to Use Gas-Mixer in the “Settings -> System Configuration -> Gas Configuration” menu.

  • Page 48: Control

    USER MANUAL │ ARA G-FL 2) Control When using the Gas-Mixer, touch the [Control] menu on the main screen to display the [Gas-Mixer Setup] menu. [Use O2 Gas]: Displays whether O2 Gas is used. (When changing to “Use”, select Always On / Auto / Always Off to set the operation of the O2 gas pump.)

  • Page 49: Main

    USER MANUAL │ ARA G-FL 3) Main When using the Gas-Mixer, the Main screen is displayed as follows 1) Gas-Mixer Status • Gas-Mixer status: You can set the gas operation status and input amount ON/OFF operation time. HANIL Scientific Inc. │ ihanil.com │ Service : +82-2-3452-8966 / techsupport@ihanil.com | 49...

  • Page 50: Mfc Usage (Option)

    MFC can be used as an option when you want to adjust the connected sensor using O2 (or AIR) / N2/ CO2 Gas in addition to the pump (ACID / BASE / FOAM / FEED) connected to G-FL. To connect and use MFC to G-FL, change MFC and gas to be used in “Settings -> System Configuration ->...

  • Page 51: Mfc Setup

    USER MANUAL │ ARA G-FL MFC Setup Set MFC dosage and operation mode. When the Control -> MFC Setup menu is selected, it is displayed as shown below. [Type]: Select the MFC Type to set. [Flow Range]: Shows the Flow Range value set in the MFC Configuration menu.
  • Page 52 im-ara.com...

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